Congenital stationary night blindness is associated with the leopard complex in the Miniature Horse     

Sandmeyer LS  Bellone RR  Archer S  Bauer BS  Nelson J  Forsyth G  Grahn BH 《Veterinary ophthalmology》2012,15(1):18-22

Objective To determine if congenital stationary night blindness (CSNB) exists in the miniature horse in association with leopard complex spotting patterns (LP), and to investigate if CSNB in the miniature horse is associated with three single nucleotide polymorphisms (SNPs) in the region of TRPM1 that are highly associated with CSNB and LP in Appaloosas. Animals studied Three groups of miniature horses were studied based on coat patterns suggestive of LP/LP (n = 3), LP/lp (n = 4), and lp/lp genotype (n = 4). Procedures Horses were categorized based on phenotype as well as pedigree analysis as LP/LP, LP/lp, and lp/lp. Neurophthalmic examination, slit‐lamp biomicroscopy, indirect ophthalmoscopy, and scotopic flash electroretinography were performed on all horses. Hair samples were processed for DNA analysis. Three SNPs identified and associated with LP and CSNB in the Appaloosa were investigated for association with LP and CSNB in these Miniature horses. Results All horses in the LP/LP group were affected by CSNB, while none in the LP/lp or lp/lp groups were affected. All three SNPs were completely associated with LP genotype (χ² = 22, P << 0.0005) and CSNB status (χ² = 11, P < 0.0005). Conclusions The Miniature Horse breed is affected by CSNB and it appears to be associated with LP as in the Appaloosa breed. The SNPs tested could be used as a DNA test for CSNB until the causative mutation is determined.  相似文献   

Milled Rice Breakage Due to Environmental Conditions     

T. J. Siebenmorgen  Z. T. Nehus  T. R. Archer 《Cereal Chemistry》1998,75(1):149-152

Milled, long-grain rice was exposed to air at temperatures (T) of 20, 30, and 40°C, and relative humidities (RH) ranging from 25 to 85%. The kernels then were subjected to a breakage test to determine the extent of damage that occurred during each exposure condition. Increasing air T levels produced higher amounts of broken kernels across the RH range. Milled rice at higher moisture content (MC) levels sustained more extensive stress crack damage at low RH conditions and less stress crack damage at high RH conditions relative to milled rice at lower MC levels. Varietal differences were also present, but were overshadowed by MC effects.  相似文献   

Sheep as vectors for branched broomrape (Orobanche ramosa subsp. mutelii [F.W. Schultz] Cout.) seed dispersal          下载免费PDF全文

Emma Ginman  Jane Prider  John Matthews  John Virtue  Jennifer Watling 《Weed Biology and Management》2015,15(2):61-69

Sheep can be vectors for the long‐distance dispersal of weeds when seed becomes attached and retained in wool or survives the gastrointestinal tract. This study aimed to determine an appropriate quarantine period for sheep that minimized the risk of the long‐distance dispersal of the seeds of branched broomrape (Orobanche ramosa L. subsp. mutelii [F.W. Shultz] Cout.), a parasitic weed. Experiments with penned sheep found that the seeds that were placed on the soil surface adhered to the wool on the thigh and belly of the sheep, with most of the seeds attaching to the feet. Most of the seeds that were applied to the belly and thigh wool detached within 2 days, although a small proportion was present after 7 days. The seeds that were introduced to the digestive tract via drenching had a peak voidance 2 days later and no seed was detected on Day 8. It is suggested that a 7 day quarantine period for sheep would be sufficient in order to reduce the risk of the internal transport of seed to acceptable levels, but a small risk of the external transport of seed on the fleece remains.  相似文献   

Role of salicylic acid in phosphite-induced protection against Oomycetes; a Phytophthora cinnamomi - Lupinus augustifolius model system     

Emma Groves  Kay Howard  Giles Hardy  Treena Burgess 《European journal of plant pathology / European Foundation for Plant Pathology》2015,141(3):559-569

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Cadmium-Containing Carbonic Anhydrase CDCA1 in Marine Diatom Thalassiosira weissflogii     

Vincenzo Alterio  Emma Langella  Giuseppina De Simone  Simona Maria Monti 《Marine drugs》2015,13(4):1688-1697

The Carbon Concentration Mechanism (CCM) allows phytoplakton species to accumulate the dissolved inorganic carbon (DIC) necessary for an efficient photosynthesis even under carbon dioxide limitation. In this mechanism of primary importance for diatoms, a key role is played by carbonic anhydrase (CA) enzymes which catalyze the reversible hydration of CO₂, thus taking part in the acquisition of inorganic carbon for photosynthesis. A novel CA, named CDCA1, has been recently discovered in the marine diatom Thalassiosira weissflogii. CDCA1 is a cambialistic enzyme since it naturally uses Cd²⁺ as catalytic metal ion, but if necessary can spontaneously exchange Cd²⁺ to Zn²⁺. Here, the biochemical and structural features of CDCA1 enzyme will be presented together with its putative biotechnological applications for the detection of metal ions in seawaters.  相似文献   

Stepwise function of natural growth for Scylla serrata in East Africa: a valuable tool for assessing growth of mud crabs in aquaculture          下载免费PDF全文

Per‐Olav Moksnes  David Oersted Mirera  Emma Björkvik  Muumin Iddi Hamad  Humphrey Matalu Mahudi  Daniel Nyqvist  Narriman Jiddawi  Max Troell 《Aquaculture Research》2015,46(12):2938-2953

Predicting growth is critical in aquaculture, but models of growth are largely missing for mud crab species. Here, we present the first model of natural growth in juvenile and adult mud crabs Scylla serrata from East Africa using a stepwise growth function based on data on intermoult periods and growth at moult from field mark‐recapture, pond and laboratory studies. The results showed a sigmoid growth pattern in carapace width and suggest that S. serrata in East Africa will reach 300 g and sexual maturity ~9.9 months after settlement, and a commercial size of 500 g after 12.4 months. Analyses of the literature identified several issues with the common praxis to compare standard growth measures between aquaculture studies with different initial size or growing periods. Using the new growth function to estimate the proportional difference between modelled and obtained growth as an alternative method, we show that growth rates of S. serrata cultured in cage systems, which are dominant in East Africa, was <40% of the estimated natural growth and growth obtained in pond systems. The analysis also indicated that growth rates of S. serrata in Southeast Asia was over 50% higher compared with similar culture systems in East Africa, and that different species of mud crabs had large differences in growth rates. This study shows that growth in the present mud crab aquaculture systems in East Africa is below their expected potential. Further work is needed to identify the factors behind this observation.  相似文献   

Immunohistochemical detection of NOD1 and NOD2 in the healthy murine and canine eye     

Emma Scurrell  Rachael Stanley†  Sandra Schöniger 《Veterinary ophthalmology》2009,12(4):269-275

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The Cardiac Biomarker NT-proBNP Is Increased in Dogs with Azotemia     

E. Raffan  J. Loureiro  J. Dukes-McEwan  S. Fonfara  R. James  S. Swift  N. Bexfield  M.E. Herrtage  J. Archer 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2009,23(6):1184-1189

Background: Amino‐terminal probrain natriuretic peptide (NT‐proBNP) has been proposed as a useful biomarker for heart disease in dogs. In humans, decreased glomerular filtration rate (GFR) increases NT‐proBNP. Objective: To investigate whether decreased GFR as indicated by plasma creatinine concentration is associated with increased NT‐proBNP in dogs without heart disease. Animals: Four groups of dogs: healthy (n= 39), azotemic (n= 36), heart disease (n= 37), and congestive heart failure (CHF) (n= 7) presented to 2 teaching hospitals. Methods: Prospective observational cohort study. Plasma creatinine concentration and NT‐proBNP were measured in every dog. Nonparametric tests were used to compare the differences among groups. The median and actual results for each group were compared with the manufacturer's recommended and previously published suggestions for cut‐off values for diagnosis of heart disease. Results: Median (range) plasma creatinine concentration was 1.47 (1.06–1.70), 4.36 (1.74–15.6), 1.22 (0.69–1.91), and 1.45 (0.63–1.64) mg/dL and median (range) NT‐proBNP was 118 (2–673), 556 (37–1,819), 929 (212–5,658), and 3,144 (432–5,500) pmol/L for the healthy, azotemic, heart disease, and CHF groups, respectively. Pair‐wise comparison indicated a significant difference among all groups for NT‐proBNP (P≤ .049). Plasma creatinine concentration was significantly higher in the azotemic group compared with other groups (P < .001) but there was no significant among other groups. Application of 3 recommended cut‐off values led to misclassification of dogs with azotemia as having heart disease. Conclusions: Azotemia results in NT‐proBNP being increased to concentrations reported as diagnostic of heart disease or heart failure in dogs. Care should be employed when interpreting the results of NT‐proBNP in patients with known or possible increased plasma creatinine concentration.  相似文献   

Cerebrospinal fluid from a 6-year-old dog with severe neck pain     

Bauer NB  Bassett H  O'Neill EJ  Acke E 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):123-125

A 6-year-old, intact female, Labrador Retriever/Terrier cross was presented to the University Veterinary Hospital, University College Dublin with a 3-week history of therapy-resistant cervical pain and intermittent fever. Physical examination findings included marked cervical pain resulting in neck extension and vocalization. Examination of the CSF revealed mild pleocytosis (total nucleated cells = 0.009 x 10(9)/L, reference interval <0.005 x 10(9)/L). Cytocentrifuged preparations of the CSF were of low cellularity, containing predominantly macrophages and occasional small lymphocytes. Several small- to medium-sized fragments of a slightly granular, amorphous, eosinophilic substance were observed. The majority of mononuclear cells were located within this material, in small groups of 3-13 cells. The amorphous foamy material stained positive with Luxol fast blue, suggestive of myelin-like material. The dog was euthanized and postmortem examination revealed intervertebral disk protrusion between C2 and C3. Hematoxylin- and Luxol fast blue-stained histopathologic sections of brain and spinal cord revealed only mild hemorrhage. The extracellular material in the CSF of this dog may have been caused by myelin degeneration or leakage of phospholipids from damaged cells. Because no histologic evidence of demyelination was observed with the disk extrusion, the myelin-like material in this case was thought to be the product of phospholipid breakdown from damaged cellular membranes. Three cases of dogs with spinal cord disease and myelin-like material in the CSF have been reported previously. The clinical significance of this finding is still unknown.  相似文献   

Phenylalanine ammonia lyase activity in chilli CM-334 infected by Phytophthora capsici and Nacobbus aberrans     

Damaris Godinez-Vidal  Mario Rocha-Sosa  Edgar B. Sepúlveda-García  Joel Lara-Reyna  Reyna Rojas-Martínez  Emma Zavaleta-Mejía 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(3):299-303

We tested the hypothesis that PAL activity in chilli plants CM-334 inoculated with Nacobbus aberrans (Na) alone or in combination with Phytophthora capsici (Pc), is lower than in those inoculated only with Pc. At 21 days after nematode inoculation, inoculated plants showed a significant (P < 0.01) reduction of 48% in PAL activity compared to those non-inoculated in two separate experiments. In two other tests, where plants were inoculated with the oomycete 21 days after inoculation with the nematode, PAL activity at 2, 4, 6, 8 and 24 h after inoculation with Pc was significantly higher (Tukey, P < 0.01) in plants inoculated only with Pc than in plants inoculated only with Na or both pathogens (Na+Pc).  相似文献