Towards the identification of ecological management units: A multidisciplinary approach for the effective management of bottlenose dolphins in the southern Iberian Peninsula          下载免费PDF全文

Joan Giménez  Marie Louis  Enrique Barón  Francisco Ramírez  Philippe Verborgh  Pauline Gauffier  Ruth Esteban  Ethel Eljarrat  Damià Barceló  Manuela G. Forero  Renaud de Stephanis 《水产资源保护:海洋与淡水生态系统》2018,28(1):205-215

Survival and growth of geoduck clam larvae (Panopea generosa) in flow‐through culture tanks under laboratory conditions          下载免费PDF全文

Gabriel Enrique Nava‐Gómez  Zaúl Garcia‐Esquivel  Eugenio Carpizo‐Ituarte  Tatiana N Olivares‐Bañuelos 《Aquaculture Research》2018,49(1):294-300

The geoduck clam, Panopea generosa, is a species from the west coast of Baja California, Mexico, and the optimization of seed production systems is still a limiting factor for its aquaculture. In this study, a flow‐through culture system was designed and tested in P. generosa larvae. Survival and growth was compared in triplicate 45‐L fiberglass tanks using three larval densities (5, 10 and 15 larvae/ml). A head tank kept constant the water inflow, with a daily renewal rate of 1.8× tank volume. The food (Isochrysis spp.) was dosed according to the ingestion rate of larvae and the dilution rate. Survival decreased linearly during the first 10 days and reached asymptotic values of ca. 20% (15 larvae/ml) and 50% (densities of 5 and 10 larvae/ml) afterwards. Mean shell length at the end of the experiment (243 ± 1.8 to 270 ± 0.7 μm) was not statistically different among treatments, even though a trend towards higher gross growth rate was observed in the treatment with the lowest density (9.5 μm/day) relative to the rest of the treatments (8.5 μm/day). It is concluded that P. generosa larvae can be successfully grown in flow‐through systems at maximum densities of 10 larvae/ml without significantly affecting their survival and growth rates. The system design was reliable, kept a constant water flow with reduced maintenance, and may represent an important option in the laboratory for increasing the stocking density of Panopea species during the larval phase.  相似文献   

Use of oral fluralaner for the treatment of Psoroptes cuniculi in 15 naturally infested rabbits          下载免费PDF全文

Galia Sheinberg  Camilo Romero  Rafael Heredia  Miguel Capulin  Enrique Yarto  Juan Carpio 《Veterinary dermatology》2017,28(4):393-e91

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Molecular cloning, expression and first antigenic characterization of human astrovirus VP26 structural protein and a C-terminal deleted form     

Royuela E  Sánchez-Fauquier A 《Comparative immunology, microbiology and infectious diseases》2010,33(1):1-14

The open reading frame 2 (ORF2) of human astrovirus (HAstV) encodes the structural VP26 protein that seems to be the main antigenic viral protein. However, its functional role remains unclear. Bioinformatic predictions revealed that VP29 and VP26 proteins could be involved in virus-cell interaction. In this study, we describe for the first time the cloning and expression in Escherichia coli (E. coli) of a recombinant VP26 (rVP26) protein and a VP26 C-terminal truncated form (VP26 Delta C), followed by purification by NTA-Ni(2+) agarose affinity chromatography. Protein expression and purification were evaluated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot (WB). Then, the purified proteins were evaluated for antigenic properties in enzyme linked immunosorbent assay (ELISA) using a polyclonal antibody (PAb) and a neutralizing monoclonal antibody (nMAb) named PL2, both of them directed to HAstV. The results presented herein indicate that the C-terminal end of the VP26 protein is essential to maintain the neutralizing epitope recognized by nMAb PL2 and that the N-terminus of VP26 protein may contain antigenic lineal-epitopes recognized by PAb. Thus, these recombinant proteins can be ideal tools for further antigenic, biochemical, structural and functional VP26 protein characterization, in order to evaluate its potential role in immunodiagnosis and vaccine studies.  相似文献   

Nutrients return from leaves and litterfall in a mediterranean cork oak (<Emphasis Type="Italic">Quercus suber</Emphasis> L.) forest in southwestern Spain     

Enrique Andivia  Manuel Fernández  Javier Vázquez-Piqué  Aranzazu González-Pérez  Raúl Tapias 《European Journal of Forest Research》2010,129(1):5-12

The knowledge of the cycle of nutrients is fundamental for the correct comprehension of the tree–soil relationship and for an adequate forest management. In order to analyse the nutrients return from leaves and litterfall in a Mediterranean cork oak forest in southwestern Spain, 12 trees were randomly selected and litterfall collected for 2 years. Samples were taken monthly and separated in different fractions (leaves, twigs, catkins, acorns and miscellaneous), then leaves nutrients were analyzed. Simultaneously, we analyzed the nutrient content of living leaves from the same trees in each season during 1 year. The analyzed nutrients were C, N, P, K, Ca, Mg, Fe, Mn, S, Cu, Zn and Mo. Annual patterns of each nutrient in fallen leaves were characterized and compared with seasonal values of these nutrients in living leaves. Leaves fall has two annual maximum, first and most important in spring around April coinciding with renewal of foliar cover and second around October. Main concentration patterns of N, P and K are related with phenological patterns, in consequence minimum concentration in leaves fall were obtained in periods of growing and maximum litterfall. Concentrations of Ca, Fe and Mn increase with the age of the leaves and maximum concentrations were obtained before periods of maximum litterfall while concentrations of Cu, Mo and Mg stay stable. Seasonal analysis of nutrients in living leaves collected from the same trees in four different periods of the year allowed to corroborate the patterns of leaves fall and the probable osmotic function of K.  相似文献   

First report of Bemisia tabaci Mediterranean (Q biotype) species in Brazil          下载免费PDF全文

Leonardo da Fonseca Barbosa  Valdir Atsushi Yuki  Julio Massaharu Marubayashi  Bruno Rossitto De Marchi  Fernando Luis Perini  Marcelo Agenor Pavan  Danielle Ribeiro de Barros  Murad Ghanim  Enrique Moriones  Jesus Navas‐Castillo  Renate Krause‐Sakate 《Pest management science》2015,71(4):501-504

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Operational interactions between South American sea lions and gillnet fishing in southern Brazil          下载免费PDF全文

Paulo Henrique Ott  Ignacio Benites Moreno  Daniel Danilewicz  Maurício Tavares  Enrique Alberto Crespo  Salvatore Siciliano  Larissa Rosa De Oliveira 《水产资源保护:海洋与淡水生态系统》2016,26(1):108-120

Distribution and frequency of resistance to four herbicide modes of action in Lolium rigidum Gaud. accessions randomly collected in winter cereal fields in Spain     

Iñigo Loureiro  Enrique Rodríguez-García  Concepción Escorial  José María García-Baudín  José Luis González-Andújar  María Cristina Chueca 《Crop Protection》2010

Lolium rigidum is the most prevalent and damaging grass weed of winter cereals in Spain. L. rigidum infestations are frequently treated with herbicides and, consequently, populations have evolved resistance. The objective of this study was to determine the extent and frequency of herbicide resistance in L. rigidum populations in Spain to the selective herbicides chlortoluron, diclofop-methyl and chlorsulfuron, commonly used for its control in-crop, and to glyphosate. The response to these herbicides was evaluated on 123 accessions surveyed randomly across cereal cropping areas of the regions of Castile and León, Catalonia and Andalusia. The fresh weight and the frequency of undamaged plants were calculated for each accession and herbicide. At the regional level, higher frequencies of accessions displaying resistance occurred in Catalonia, an intensively cropped region with a greater herbicide selection pressure. Of concern is that in this region the 60% of the accessions displayed some level of resistance to the ALS-inhibiting herbicide chlorsulfuron. The 6.9% of the accessions found in Castile and León with some resistance to glyphosate could also indicate an incipient problem of resistance to this herbicide. For the other herbicides and regions the majority of the accessions remained susceptible. The possible mechanisms of herbicide resistance development in L. rigidum accessions (target-site versus non-target-site resistance) and their variation among regions was discussed. This study can be used to generate herbicide resistance-management schemes for farmers, based upon the herbicide the site and the potential for resistance development.  相似文献   

The effect of petrocalcic horizons on the content and distribution of organic carbon in a Mediterranean semiarid landscape     

José L. Díaz-Hernández  Enrique Barahona Fernández 《CATENA》2008

Petrocalcic horizons are frequent in soils of semiarid landscapes. A survey of SIC and SOC contents made in Southern Spain in a pilot area with well defined geomorphological surfaces showed that topsoils overlying petrocalcic horizons are almost twice as rich in SOC as soil of similar depth without petrocalcic horizons. This could be due to impedance to root penetration, changes in redox potential and soil water availability caused by the presence of indurated crust. Soil age, on the contrary, seems not be an essential factor, since only a short time is required to reach a steady state in SOC in comparison to the time span available for soil formation on the different geomorphic surfaces.  相似文献   

Modeling the Requirements of Agricultural Limestone in Acid Sulfate Soils of Brazil and Colombia     

Enrique Combatt Caballero  Alfredo Jarma Orozco  Manuel Palencia Luna 《Communications in Soil Science and Plant Analysis》2019,50(8):935-947

The aim of this study was to model the determination of the amount of agricultural limestone necessary to correct acidification in acid sulfate soils (ASS). This study used 14 equations and the chemical variables of the soils including pH, soil organic matter (SOM), potential acidity (H+ Al), sum of bases (BS), potential acidity (T), base saturation (V) and exchangeable Al with KCl. The results indicated that in the studied, the lowest requirements for doses of limestone (DL) were described by the equations DL = 2.906((H+ Al) (45-V)/100)°⁸⁰, DL = 0.08 + 1.22Al_KCl, DL = 0.6T – BS, and DL = 1.5Al_KCl, with mean values of 29.5, 30.5, 31.5 and 37.4 t ha^?1. In addition, the models with the best correlation for determining the limestone requirements to obtain 80% of the maximum production of sorghum (dry matter) were DL = ?1.43 + 0.9237 (SOM (6-pH)) and DL = ?0.022 + 2.7425 ((H+ Al) (45-V)/100).  相似文献