Effect of Daily Food Supplementation with Essential Fatty Acids on Canine Semen Quality     

AA da Rocha  ICN da Cunha  BB Ederli  AP Albernaz  CR Quirino 《Reproduction in domestic animals》2009,44(S2):313-315

Polyunsaturated fatty acids are important membrane components that influence membrane integrity and fluidity. In the present study, the effect of oral supplementation for 60 days with essential fatty acids (omega 3, 6 and 9) and vitamin E on canine semen quality was evaluated. Sixteen dogs were selected for the experiment; eight were used as the control group and eight received the fatty acid supplemented diet for 60 days. Semen samples were taken every 15 days during the entire experimental period and were analyzed for volume (ml), motility (%), vigour (0–5), concentration (×10⁶/ml), morphology of spermatozoa (%), plasma membrane integrity (%; using the hyposmotic swelling test) and thermoresistance (motility and vigour after 4 h at 38°C). We concluded that, daily supplementation with omega 3, omega 6 and omega 9 fatty acids, together with vitamin E, for a period of 60 days, significantly increased the semen volume of the treated group after 15 days of supplementation; the vigour and concentration of spermatozoa were superior after the first month of supplementation, while the percentage of morphologically abnormal spermatozoa decreased and the cells were protected against thermal stress.  相似文献   

Acid–Base Changes in Canine Neonates Following Normal Birth or Dystocia     

CF Lúcio  LCG Silva  JA Rodrigues  GAL Veiga  CI Vannucchi 《Reproduction in domestic animals》2009,44(S2):208-210

There are limited data concerning blood gas parameters in neonatal dogs. Knowledge of the normal physiology may facilitate effective therapeutic intervention and potentially reduce neonatal mortality. This study examined acid–base parameters in pups born at normal parturition (n = 27) compared with those born after obstetrical assistance or caesarean operation (n = 13) and those born following oxytocin (OXY) administration for treatment of uterine inertia (n = 11). Pups were subjected to an objective scoring method of neonatal health adapted from use in humans (the Apgar score) at birth and again at 5 and 60 min after birth. Venous blood samples were collected at 5 and 60 min after birth for evaluation of blood gas parameters. At birth, all pups had low Apgar scores and a mixed acidosis. The base excess was lowest for pups delivered after OXY administration. The Apgar score improved for all pups after 5 min of birth and there was an improvement in carbon dioxide tension, base excess and venous blood pH at 1 h, although in all pups a metabolic acidosis persisted. These data provide an important insight into neonatal physiology and the variability of blood gas parameters in pups born at normal and abnormal parturition and provide the basis for clinical decision making following dystocia.  相似文献   

Systemic alterations of bovine hemostasis due to Rhipicephalus (Boophilus) microplus infestation     

Reck J  Berger M  Terra RM  Marks FS  da Silva Vaz I  Guimarães JA  Termignoni C 《Research in veterinary science》2009,86(1):56-198

The tick Rhipicephalus (Boophilus) microplus is a hematophagous ectoparasite that causes considerable economic losses to cattle breeding. Although R. microplus saliva contains several molecules that interfere with the blood coagulation process, so far the systemic alterations in the host hemostatic system have not been described. This study aims to determine if R. microplus infestation induces any disturbance to the host’s hemostatic system. To address these questions, six calves were experimentally infested with 20,000 R. microplus larvae and their blood was collected before and 7, 14 and 21 days post-infestation. Collagen and ADP-induced platelet aggregation as well as coagulation (activated partial thromboplastin time and prothrombin time) decreased in infested bovines. Platelet blood count and fibrinogen increased during the course of infestation, probably as a compensatory response. These alterations may play a role in host health status, and show that the host cannot fully counteract the tick anti-hemostatic mechanisms.  相似文献   

Distribution of antibodies against porcine circovirus type-2 (PCV2) in single site and multi-site farrow-to-finish farms in Brazil     

Priscilla F. Gerber  Grazielle C.F. Galinari  Marcos X. Silva  Fbia S. Campos  Adrienny C.T. Reis  Zlia I.P. Lobato 《Research in veterinary science》2009,87(3):488-491

A comparative serologic study was performed in seven single site (SS) farrow-to-finish farms and four multi-site (MS) farrow-to-finish farms, with or without post-weaning multisystemic wasting syndrome (PMWS). In each farm, 30 blood samples were collected for each category of the production cycle: sows, farrowing crate, nursery, grower pigs, and finishing pigs. Sera were evaluated for the presence of antibodies to porcine circovirus type-2 (PCV2) via immunoperoxidase monolayer assay. Serologic profiles for PCV2 were different between SS and MS farms. Seroconversion following the decline in maternal antibodies occurred at a later stage on SS farms (grower pigs) than MS farms (nursery pigs). MS farms tended to have lower antibody titers than SS farms in the categories of sow, piglet, and nursery, while higher antibody titers were found in grower pigs. Characterization of serologic profiles for different farms may provide important information for the adoption of vaccination programs.  相似文献   

Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs     

Siqueira AK  Ribeiro MG  Leite Dda S  Tiba MR  Moura Cd  Lopes MD  Prestes NC  Salerno T  Silva AV 《Research in veterinary science》2009,86(2):206-210

The aim of this study was to compare the prevalence of virulence genes in 158 Escherichia coli strains isolated from 51 clinical cases of UTIs, 52 of pyometra and from 55 fecal samples from healthy dogs by PCR. papC was found in 12 (23.5%) strains isolated from UTIs, 19 (36.5%) from pyometra and 10 (18.2%) from feces. papGII was observed in 3 (5.8%) strains from pyometra, and papGIII in 10 (19.6%) from UTIs, 15 (28.8%) from pyometra and 9 (16.4%) from feces. sfaS was detected in 22 (43.1%) strains from UTIs, 24 (46.1%) from pyometra and 19 (34.5%) from feces. hlyA was observed in 17 (33.3%) strains from UTIs, 18 (34.6%) from pyometra and 7 (12.7%) from feces, while cnf-1 was detected in 11 (21.6%) from UTIs, 21 (40.4%) from pyometra and 9 (16.4%) from feces. iucD was observed in 12 (23.5%) strains from UTIs, 9 (17.3%) from pyometra and 1 (1.8%) from feces. usp was found 17 (33.3%) isolates from UTIs and 36 (69.9%) from pyometra.  相似文献   

Phenotypic and genotypic characterization of Prototheca zopfii in a dog with enteric signs     

Mrcio Garcia Ribeiro  Marconi Rodrigues de Farias  Uwe Roesler  Katja Roth  Sabrina Mari Rodigheri  Marco Antonio Ostrowsky  Tatiana Salerno  Amanda Keller Siqueira  Marta Catarina Fernandes 《Research in veterinary science》2009,87(3):479-481

This is a case report of enteric protothecosis caused by Prototheca zopfii in an eight-year-old male mixed breed dog with a history of chronic bloody diarrhea, loss of appetite and weight loss. Algae were isolated from rectal scrapings in defibrinated sheep blood agar and dextrose Sabouraud agar. Cytological evaluation showed the presence of globular and cylindrical organisms with a defined capsule and variable number of endospores, characteristic of the genus Prototheca, in the rectum of the animal. Scanning electron microscopy of P. zopfii strains at different development stages confirmed the diagnosis of algal infection. Molecular identification using a conserved 18S rDNA gene sequence determined that the strain belonged to genotype 2. This report describes success on treatment of canine protothecosis, diagnosed based on clinical, cytological, microbiological, scanning electron microscopy and genotypical findings.  相似文献   

The role of arbuscular mycorrhizal fungi and cattle manure in the establishment of Tocoyena selloana Schum. in mined dune areas     

Renata Gomes de Souza  Bruno Tomio Goto  Danielle Karla Alves da Silva  Fábio Sérgio Barbosa da Silva  Everardo V.S.B. Sampaio  Leonor Costa Maia 《European Journal of Soil Biology》2010,46(3-4):237-242

In mined dune areas, revegetation with manured seedlings of native species is a common practice. Establishment of mycorrhized Tocoyena selloana seedlings in the mined coastal sand dunes of Northeast Brazil was tested. In greenhouse, seedlings were grown in substrates with 0, 5, 10, 15 or 20% cattle manure proportions and inoculated with Acaulospora longula, a mixture of native arbuscular mycorrhizal fungi (AMF) or uninoculated. The seedlings responded positively to the inoculation, but growth was limited in the absence of manure, independently of inoculation, and was higher with fertilizing doses ≥10%. The seedlings transplanted to the field were grown in a substrate with 16.5% manure and inoculated with A. longula or Gigaspora albida. After 13 months, 19 AMF species were identified in the rhizosphere and the inoculated plants were more colonized than those uninoculated. Plants associated to G. albida were taller and those associated to A. longula had a tendency of higher biomass than the uninoculated ones. Even though this tendency was not statistically significant, considering the effect on height and the low cost of inoculation it may be a feasible practice to maximize environment restoration.  相似文献   

Higher breeding densities of the threatened little bustard Tetrax tetrax occur in larger grassland fields: Implications for conservation     

João Paulo Silva  Jorge M. Palmeirim 《Biological conservation》2010,143(11):2553-2558

Previous studies have found that densities of little bustard Tetrax tetrax breeding males tend to be higher in areas with smaller agricultural fields, presumably due to increased habitat diversity. However, exceptionally high densities have been found in large grassland fields in Portugal, which suggests that the influence of field size varies geographically, and that the role of this factor is not yet fully understood, despite its importance as a key management issue.We studied how field size, together with vegetation structure, influences the presence and density of breeding little bustards in a region of southern Portugal. Fifty-four grassland fields were sampled in 2007 and another 29 in 2008, with sizes ranging from 23 to 172 ha. A total of 183 breeding males were found in 47 of these fields, reaching densities of up to 37 males/100 ha. A higher probability of occurrence of breeding males was found in larger fields with a vegetation height below 40 cm and field size alone explained 46% of the variability in male density. These results suggest that larger continuous areas of suitable habitat attract many males, most likely as a consequence of their lek mating system. We conclude that conservation efforts, in a landscape context of large farm sizes, should: (1) be channelled to farms with large fields; (2) ensure adequate livestock grazing to create suitable habitat and (3) promote management at a landscape level to ensure the most continuous grassland habitat patches possible.  相似文献   

Evaluation of Total and Faecal Coliforms and Salmonella spp. in Irradiated Mussels from Brazil     

Flaivia Aline Andrade Calixto  Eliana de Faitima Marques de Mesquita  Robson Maia Franco  Cynthia Annes Rubiao  Neila Mello dos Santos Cortez  Mauro Carlos Lopes Souza  Licinio Esmeraldo da Silva 《农业科学与技术》2012,(4):511-519

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Characterization of Pro-embryogenic Calli and Somatic Embryogenesis of Byrsonima intermedia A. Juss.     

Luciano Coutinho Silva  Renato Paiva  Diogo Pedrosa Correa Da Silva  Sandro Barbosa  Rairys CravoHerrera  Lisete Chamma Davide  Patricia Duarte De Oliveira Paiva 《农业科学与技术》2012,(8):962-970

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