Spontaneous Malignant T Cell Lymphoma in a Young Male Common Marmoset (Callithrix jacchus)     

Itaru Yamaguchi  Kensuke Myojo  Hiroko Sanada  Eri Sudo  Sayaka Ootsuka  Hiroshi Okumura  Atsuko Takami  Tomomi Yoneshige  Yui Suzuki  Minami Imaizumi  Chie Takada  Naoya Kimoto  Koji Saeki  Katsumi Takaba 《Journal of toxicologic pathology》2013,26(3):301-307

We histopathologically and immunohistochemically investigated a case of malignant lymphoma that spontaneously developed in a male common marmoset at two years of age. Beginning at two years four months of age, the animal had an enlargement of the submandibular and inguinal lymph nodes, small subcutaneous nodules near the right breast and an approximately fivefold increase in peripheral lymphocyte count compared with the previous examination value. The postmortem findings at two years eight months of age showed lymphadenopathy with enlargement of the thymus and spleen. Small- to intermediate-sized neoplastic lymphocytes had diffusely proliferated in the enlarged nodes. The neoplastic cells were pleomorphic and had irregularly shaped nuclei. The nuclear chromatin staining revealed hyperchromatism in the small-sized cells, and the intermediate-sized cells exhibited vesicular staining. An immunohistochemical examination indicated that the neoplastic lymphocytes were positive for CD3 and negative for CD20, thus suggesting that they had originated from T cells. In addition, the proliferation of high endothelial venules and reactive epithelioid histiocytes was observed. Scattered tingible body-laden macrophages were infrequently detected. Neoplastic lymphocytes were also observed in the thymus, spleen, heart, lungs, liver, kidneys, adrenal glands and femoral and sternal bone marrow. This malignant lymphoma in a young male common marmoset was considered to fit the category of “peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS)” according to the new WHO system of classification.  相似文献   

Development of a screening method for genetically modified soybean by plasmid-based quantitative competitive polymerase chain reaction     

Shimizu E  Kato H  Nakagawa Y  Kodama T  Futo S  Minegishi Y  Watanabe T  Akiyama H  Teshima R  Furui S  Hino A  Kitta K 《Journal of agricultural and food chemistry》2008,56(14):5521-5527

A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-time PCR method used for the quantification of RRS in Japan. A plasmid was constructed as a competitor plasmid for the detection and quantification of genetically modified soy, RRS. The plasmid contained the construct-specific sequence of RRS and the taxon-specific sequence of lectin1 (Le1), and both had 21 bp oligonucleotide insertion in the sequences. The plasmid DNA was used as a reference molecule instead of ground seeds, which enabled us to precisely and stably adjust the copy number of targets. The present study demonstrated that the novel plasmid-based QC-PCR method could be a simple and feasible alternative to the real-time PCR method used for the quantification of genetically modified organism contents.  相似文献   

Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility     

Yoshitaka Kanaoka  Daichi Kuniyoshi  Eri Inada  Yohei Koide  Yoshihiro Okamoto  Hideshi Yasui  Yuji Kishima 《Plant methods》2018,14(1):102

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Acknowledgment to the Reviewers     

Eri Matsuura 《Soil Science and Plant Nutrition》2016,62(5-6):574-575

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Preparation of Photocrosslinked Fish Elastin Polypeptide/Microfibrillated Cellulose Composite Gels with Elastic Properties for Biomaterial Applications     

Shinya Yano  Megumi Mori  Naozumi Teramoto  Makoto Iisaka  Natsumi Suzuki  Masanari Noto  Yasuko Kaimoto  Masashi Kakimoto  Michio Yamada  Eri Shiratsuchi  Toshiaki Shimasaki  Mitsuhiro Shibata 《Marine drugs》2015,13(1):338-353

Photocrosslinked hydrogels reinforced by microfibrillated cellulose (MFC) were prepared from a methacrylate-functionalized fish elastin polypeptide and MFC dispersed in dimethylsulfoxide (DMSO). First, a water-soluble elastin peptide with a molecular weight of ca. 500 g/mol from the fish bulbus arteriosus was polymerized by N,N′-dicyclohexylcarbodiimide (DCC), a condensation reagent, and then modified with 2-isocyanatoethyl methacrylate (MOI) to yield a photocrosslinkable fish elastin polypeptide. The product was dissolved in DMSO and irradiated with UV light in the presence of a radical photoinitiator. We obtained hydrogels successfully by substitution of DMSO with water. The composite gel with MFC was prepared by UV irradiation of the photocrosslinkable elastin polypeptide mixed with dispersed MFC in DMSO, followed by substitution of DMSO with water. The tensile test of the composite gels revealed that the addition of MFC improved the tensile properties, and the shape of the stress–strain curve of the composite gel became more similar to the typical shape of an elastic material with an increase of MFC content. The rheology measurement showed that the elastic modulus of the composite gel increased with an increase of MFC content. The cell proliferation test on the composite gel showed no toxicity.  相似文献   

Winter nitrate uptake by the temperate deciduous tree <Emphasis Type="Italic">Quercus serrata</Emphasis>     

Miki U. Ueda  Eri Mizumachi  Naoko Tokuchi 《Journal of Forest Research》2010,15(6):411-414

Winter nitrogen use in deciduous species is largely uncharacterized. We investigated nitrate uptake in the fine roots of a deciduous oak (Quercus serrata Thunb. ex. Murray). We conducted a ¹⁵N-labeling experiment using saplings of Q. serrata in the winter. During three weeks of labeled nitrate application, the concentration of ¹⁵N in the fine roots increased significantly. The amount of nitrogen absorbed, as nitrate, was 1.16 ± 1.02 mg N g DW⁻¹, equivalent to 7.6 ± 5.8% of the total nitrogen content. Our results indicate that Q. serrata saplings have significant potential for nitrate uptake in the fine roots in midwinter (i.e., in the absence of leaves). Although a significant amount of nitrogen applied as nitrate was accumulated, nitrate concentration in the fine roots remained low during the labeling period. Furthermore, significant nitrate reductase activity was detected. These data suggest that Q. serrata saplings can assimilate nitrate in the fine roots in midwinter.  相似文献   

Purulent Necrotic Dislocation of the Hip Joint Associated with Umbilical Infection in a Foal     

Fumie MAGATA  Mitsuo ISHII  Eri OIKAWA  Hidefumi FURUOKA  Kazutaka YAMADA  Naoki SASAKI  Syo SHIMIZU  Hisashi INOKUMA 《Journal of Equine Science》2010,21(2):17-20

A 42-day-old heavy draft horse fell into sudden astasia. Significant swelling and heat sensation of the left femoral region were observed. Because of a friction sound in the left hip, we supposed that the hip joint was dislocated or the hip bone was fractured. Computed Tomography (CT) examination showed that the left hip joint was dislocated and the left femoral head was disjunct. We carried out a pathological autopsy, and made a diagnosis of the foal as fracture of the hip bone and femoral head with suppurative umbilical arteritis. Pathologic changes in the umbilical artery and hind leg were completely unilateral, suggesting that left umbilical arteritis spread to the blood circulation, causing arthritis and dislocation of the hip bone.  相似文献   

Morphological acclimation to understory environments in Abies amabilis, a shade- and snow-tolerant conifer species of the Cascade Mountains, Washington, USA   总被引：2，自引：0，他引：2  

Mori AS  Mizumachi E  Sprugel DG 《Tree physiology》2008,28(5):815-824

Light-related plasticity in a variety of crown morphology and within-tree characteristics was examined in sun and shade saplings of Abies amabilis Dougl. ex J. Forbes growing in two late-successional forests with different snow regimes in the Cascade Mountains of Washington, USA. Compared with sun saplings, shade saplings typically had broad flat crowns as a result of acclimation at several scales (needle, shoot, branch, crown and whole sapling). Shoots of shade saplings had a smaller needle mass per unit of stem length than shoots of sun saplings, a feature that enhances light-interception efficiency by reducing among-needle shading. The low annual rate of needle production by shade saplings was associated with a longer needle lifespan and slower needle turnover. Reduced needle production within a shoot was reflected at the branch level, with lateral branches of shade saplings having a smaller needle mass than branches of the same length of sun saplings. Reduced allocation to needles permits greater investment in branches and stems, which is necessary to support the horizontally expanding branch system characteristic of shade saplings. Mean branch age of shade saplings was significantly higher than that of sun saplings. Shade saplings had lower needle mass per unit of trunk biomass or total biomass, reflecting greater investment in the trunk as a support organ. Increased investment in support organs in shade was more evident in the snowier habitat. The observed morphological acclimation makes A. amabilis highly shade and snow-tolerant and thus able to dominate in many late-successional forests in snowy coastal mountain regions.  相似文献   

An Elicitor from Botrytis cinerea Induces the Hypersensitive Response in Arabidopsis thaliana and Other Plants and Promotes the Gray Mold Disease     

Govrin EM  Rachmilevitch S  Tiwari BS  Solomon M  Levine A 《Phytopathology》2006,96(3):299-307

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Development of bio-assays and screening for resistance to beet armyworm (Spodoptera Exigua Hübner) in Allium cepa L. and its wild relatives     

Sijun Zheng  Betty Henken  Willem Wietsma  Eri Sofiari  Evert Jacobsen  Frans A. Krens  Chris Kik 《Euphytica》2000,114(1):77-85

The beet armyworm (Spodoptera exigua Hübner)is the most important pest in tropical Alliumcultivations. All shallot (Allium cepa L. group Aggregatum) cultivars are susceptible to this pest. Therefore accessions from three wild Alliumspecies, namely A. galanthum Kar. et Kir., A. fistulosum L. and A. royleiStearn, next to A. cepa L. were used to screen for resistance. First of all, a reliable bio-assay had to be developed. To this end transparent plastic cages with in total 5 plants of one accession per cage were placed on per lite in a heated greenhouse. Five 3-day old larvae were inoculated on each plant. Eight days after inoculation the number of surviving larvae per cage and the mean fresh weightper larva was determined. The lowest larval survival (36%) was found on A. roylei. This was not, however, significantly different from other Allium accessions. Significant differences were found in the fresh weight per larva fed on different Allium accessions. The larvae survived on A. roylei had a very low fresh weight (10.3 mg per larva), while those on an accession of A. fistulosum had the highest fresh weight (45.1 mg per larva). The larval fresh weight on A. roylei was lower than all the other accessions except from the tropical shallot cultivar Bawang Bali. To check whether or not a toxic compound was involved in the resistance present in A. roylei, tenaccessions from four Allium species were screened. Five 3-day old larvae were inoculated on regularly replaced leaf material of each accession ofAllium species. No significant differences were found in mean fresh weight per larva and mean survival of larvae among different accessions. There were also no significant differences in pupal weight and developmental time. All larvae became pupae 10 days after inoculation. The data indicate that there is no toxic compound present in A. roylei. These results are underlined by the observation in the greenhouse bio-assay that A. roylei plants were equally damaged by the beet armyworm compared to otherAllium species. The results obtained so far therefore suggest that introduction of resistance to S. exigua via the exploitation of variation for resistance to the beet armyworm in A. roylei is unclear and that genetic engineering using Cry sequences could provide a way forward. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献