Outbreaks of severe myositis in cultured white sturgeon (Acipenser transmontanus L.) associated with Streptococcus iniae     

Felipe Pierezan  Khalid Shahin  Taylor I. Heckman  June Ang  Barbara A. Byrne  Esteban Soto 《Journal of fish diseases》2020,43(4):485-490

Outbreaks of an infectious disease affecting cultured white sturgeon (Acipenser transmontanus) were investigated. Clinical signs included erratic swimming, arching of the back and mortality. Necropsy findings included poorly demarcated yellow to dark-red and friable lesions in the epaxial muscle, ulcerative skin lesions and haemorrhages in the swim bladder and coelomic wall. Histological evaluation revealed areas of necrotizing and heterophilic myositis with aggregates of bacterial cocci. The lumen of blood vessels in the dermis, under ulcerated areas, and in the posterior kidney, was occluded by fibrin thrombi. Aggregates of Gram-positive cocci were observed in the muscle lesions and within the fibrin thrombi in the dermis and kidney. Genetically homogeneous Streptococcus iniae strains were recovered from affected fish from different outbreaks. The isolates shared high degree of similarity at gene locus (gyrB) with previously characterized S. iniae from cultured fish in California, confirming the emergence of this particular strain of S. iniae in US aquaculture.  相似文献   

Aeromonas salmonicida infects Atlantic salmon (Salmo salar) erythrocytes     

Katherinne Valderrama  Manuel Soto‐Dvila  Cristopher Segovia  Ignacio Vsquez  My Dang  Javier Santander 《Journal of fish diseases》2019,42(11):1601-1608

Aeromonas salmonicida subsp. salmonicida (hereafter A. salmonicida) is the aetiological agent of furunculosis in marine and freshwater fish. Once A. salmonicida invade the fish host through skin, gut or gills, it spreads and colonizes the head kidney, liver, spleen and brain. A. salmonicida infects leucocytes and exhibits an extracellular phase in the blood of the host; however, it is unknown whether A. salmonicida have an intraerythrocytic phase. Here, we evaluate whether A. salmonicida infects Atlantic salmon (Salmo salar) erythrocytes in vitro and in vivo. A. salmonicida did not kill primary S. salar erythrocytes, even in the presence of high bacterial loads, but A. salmonicida invaded the S. salar erythrocytes in the absence of evident haemolysis. Naïve Atlantic salmon smolts intraperitoneally infected with A. salmonicida showed bacteraemia 5 days post‐infection and the presence of intraerythrocytic A. salmonicida. Our results reveal a novel intraerythrocytic phase during A. salmonicida infection.  相似文献   

Simultaneous effect of sex and dose on efficacy of clove oil,tricaine methanesulfonate, 2‐phenoxyethanol and propofol as anaesthetics in guppies,Poecilia reticulata (Peters)          下载免费PDF全文

Olga Mitjana  Cristina Bonastre  María Teresa Tejedor  Laura Garza  Jaime Esteban  María Victoria Falceto 《Aquaculture Research》2018,49(6):2140-2146

We studied the simultaneous effect of sex and dose on anaesthesia efficacy to estimate, if possible, the lowest effective dose (LED) for clove oil, tricaine methanesulphonate (MS‐222), 2‐phenoxyethanol (2‐PE) and propofol in mature guppies. LED is the lowest dose needed to reach A5 stage in a mean time of 3 min, with mean recovery (R5) time of 5 min. We used four doses/anaesthetic: 25, 50, 75 and 100 mg/L for clove oil; 120,140,160 and 180 mg/L for MS‐222; 800, 1,000, 1,200 and 1,400 mg/L for 2‐PE, and 7.50, 8.25, 10.00 and 11.25 mg/L for propofol. Each dose was tested on 10 females and 10 males. Morbidity, mortality and behavioural changes were checked on two control groups (10 males and 10 females/group). Sedation (A3), A5 and R5 times were recorded. Significant interactive effect dose*sex on A5 time was found for each anaesthetic agent (p_dose*sex < .05). Except for MS‐222 (p_dose*sex = .284), significant interactive effect dose * sex on R5 time was found (p_dose*sex < .05). A5 time in females tended to be greater than in males, but, in general, R5 times were longer in males. Body size differences between males and females could explain these differences in MS‐222 on A5 time and for clove oil, 2‐PE and propofol on R5 time. No dose simultaneous meet LED′s conditions relating to both A5 and R5 times; therefore the lowest doses inducing A5 in a mean time of 3 min could be a safe guideline for anaesthetic procedure in both male and females.  相似文献   

Disease progression of a lethal decline caused by the 16SrIV‐D phytoplasma in Florida palms          下载免费PDF全文

B. W. Bahder  E. E. Helmick  S. Chakrabarti  S. Osorio  N. Soto  T. Chouvenc  N. A. Harrison 《Plant pathology》2018,67(8):1821-1828

Recently, a new phytoplasma was discovered in Hillsborough County in the state of Florida, USA. This phytoplasma belongs to the 16SrIV taxonomic group and is classified as subgroup D. It is the causal agent of lethal bronzing disease (LBD) of palm. Since the discovery of LBD in 2006, the disease has spread throughout much of the state. In 2014 and 2015, stands of cabbage palm and queen palms that had been present at the University of Florida's Fort Lauderdale Research and Education Center in Davie, FL began showing symptoms of LBD. After confirming the presence of the LBD phytoplasma in initially infected palms by nested PCR and RFLP analysis, all palms were systematically sampled over the period of 1 year to monitor and quantify disease spread. A total of 30 cabbage palms were tested monthly by qPCR, with five testing positive on the first sample date. By the end of the study period, 16 cabbage palms had died from the infection. A total of 16 queen palms were surveyed, with three palms initially testing positive. By the end of the study, four queen palms had tested positive and died from the infection. To the authors’ knowledge, this study is the first to document and quantify spread of palm‐infecting phytoplasmas. This data provides important insights into the ecology of palm‐infecting phytoplasmas and highlights the impact that the movement of infective insects can pose to established stands of palms.  相似文献   

Adding yeasts with sugar to increase the number of effective insecticide classes to manage Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) in cherry          下载免费PDF全文

Alan L Knight  Esteban Basoalto  Wee Yee  Rick Hilton  Cletus P Kurtzman 《Pest management science》2016,72(8):1482-1490

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Changes in Bacterial Populations During Bioremediation of Soil Contaminated with Petroleum Hydrocarbons     

Sergio Cisneros de la Cueva  César Hernández Rodríguez  Nicolás Oscar Soto Cruz  Juan Antonio Rojas Contreras  Javier López Miranda 《Water, air, and soil pollution》2016,227(3):91

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The fate of arsenic in soils adjacent to an old mine site (Bustarviejo,Spain): mobility and transfer to native flora     

Eduardo Moreno-Jiménez  Rebeca Manzano  Elvira Esteban  Jesús Peñalosa 《Journal of Soils and Sediments》2010,10(2):301-312

Background, aim, and scope  

The mobility of arsenic in soils and its transfer to other environmental components present significant environmental risks. The management of polluted land is determined by the availability, mobility, and transfer of inorganic pollutants to different ecosystem compartments. In this paper, the fate of arsenic at this mining site has been evaluated to determine future management practises to minimise such risk.  相似文献   

In silico and gene expression analysis of the acute inflammatory response of gilthead seabream (Sparus aurata) after subcutaneous administration of carrageenin     

Campos-Sánchez  Jose Carlos  Mayor-Lafuente  Javier  Guardiola  Francisco A.  Esteban  María Ángeles 《Fish physiology and biochemistry》2021,47(5):1623-1643

Fish Physiology and Biochemistry - Inflammation is one of the main causes of loss of homeostasis at both the systemic and molecular levels. The aim of this study was to investigate in silico the...  相似文献   

Sorption and thermodynamic properties of buried juvenile <Emphasis Type="Italic">Pinus sylvestris</Emphasis> L. wood aged 1,170 ± 40 BP     

Luis García Esteban  Paloma de Palacios  Francisco García Fernández  Juan Antonio Martín  Mar Génova  Juan Ignacio Fernández-Golfín 《Wood Science and Technology》2009,43(7-8):679-690

The hygroscopicity and thermodynamic properties of buried juvenile Pinus sylvestris L. wood with an age of 1,170 ± 40 BP were compared with the corresponding values of juvenile wood of the same species from recently cut trees. The 35 and 50°C isotherms were plotted following the saturated salts method and subsequently fitted in accordance with the GAB model. The isotherms were then compared by means of the hysteresis coefficients. X-ray diffractograms were used to analyse the crystal structure of the cellulose. The effect of time on the buried wood caused hemicelluloses degradation and a decrease in the crystallinity index and the crystallite length, resulting in an increase in the proportion of amorphous zones. Because of this, the equilibrium moisture contents of the buried wood are higher than of the recent wood, both in adsorption and desorption. In terms of the thermodynamic properties, the heat involved is greater in the buried wood than in the recent wood.  相似文献   

Efficacy of florfenicol for control of mortality associated with Francisella noatunensis subsp. orientalis in Nile tilapia,Oreochromis niloticus (L.)     

E Soto  S Kidd  P S Gaunt  R Endris 《Journal of fish diseases》2013,36(4):411-418

Francisella noatunensis subsp. orientalis (Fno) (syn. F. asiatica) is an emergent Gram‐negative facultative intracellular bacterium. Although it is considered one of the most pathogenic bacteria in fish, there are no commercially available treatments or vaccines. The objective of this project was to determine the most efficacious concentration of florfenicol (FFC) [10, 15 or 20 mg FFC kg^?1 body weight (bw) per days for 10 days] administered in feed to control experimentally induced infections of Fno in Nile tilapia, Oreochromis niloticus (L.), reared in a recirculating aquaculture system. The cumulative mortality of fish that received 0, 10, 15 or 20 mg FFC kg^?1 bw per day was 60, 37, 14 and 16%, respectively. Francisella noatunensis subsp. orientalis genome equivalents were detected in water from all challenged groups with slight reduction in the concentration in the florfenicol‐treated groups 4 days after treatment. The mean LOG of CFU Fno mg^?1 spleen was 3–5 and was present in all challenged groups at necropsy 11 days after treatment (21 days after challenge). Results show that florfenicol administered at doses of 15 and 20 mg FFC kg^?1 bw per days for 10 days significantly reduced mortality associated with francisellosis in Nile tilapia.  相似文献