Comparing dermoscopy and histological examination of normal equine skin          下载免费PDF全文

Lara M. Tomich  Jason B. Pieper  Adam W. Stern 《Veterinary dermatology》2018,29(2):170-e63

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Uptake of milk with and without solid feed during the monogastric phase: Effect on fibrolytic and methanogenic microorganisms in the gastrointestinal tract of calves          下载免费PDF全文

Cesar E. Guzman  Lara T. Bereza‐Malcolm  Bert De Groef  Ashley E. Franks 《Animal Science Journal》2016,87(3):378-388

Microbial communities are affected by diet and play a role in the successful transition from milk to a solid diet. The response of microorganisms in the gastrointestinal tract of Holstein bull calves to the uptake of milk with solid feed (control treatment; CT), or milk without solid feed (milk‐only treatment; MT) during the first 3 weeks of life was investigated. Samples were collected from the rumen (fluid and tissue), abomasum (fluid), cecum (fluid and tissue) and feces at 7, 14 and 20 days of age. Calf weight was higher on days 14 and 20 in the MT than the CT. In the rumen at 14 days, the fibrolytic bacteria Fibrobacter succinogenes and Prevotella ruminicola increased in the CT and Ruminococcus flavefaciens increased in the MT. This suggests that R. flavefaciens is not strictly fibrolytic and that it might use milk as a substrate or other microbial species might supply a substrate. Diet affected methanogens, but this may have been due to an indirect effect via an association with Geobacter spp. or other syntrophic partners. The treatments also affected microorganisms in the abomasum, cecum and feces. Our results contribute to an understanding of diet, microbes in the gastrointestinal tract and weaning.  相似文献   

How do field margins contribute to the functional connectivity of insect-pollinated plants?     

Clémence Guiller  Laurence Affre  Cécile Hélène Albert  Thierry Tatoni  Estelle Dumas 《Landscape Ecology》2016,31(8):1747-1761

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Practical transfusion medicine for the small animal practitioner by Bernard F. Feldman, Carolyn A. Sink     

Dorian Lara  BS    Sean Owens  DVM  Dipl. ACVP 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(1):140-140

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Plasma concentrations of lidocaine in dogs following lidocaine patch application     

Ko J  Weil A  Maxwell L  Kitao T  Haydon T 《Journal of the American Animal Hospital Association》2007,43(5):280-283

Transdermal absorption of lidocaine was determined by measuring plasma lidocaine concentrations following skin application of 5% lidocaine patches. Two lidocaine patches were placed on the ventral abdominal midline of seven dogs for 72 hours. Lidocaine was detectable in plasma 12 hours after patch application, and it reached steady-state concentrations between 24 and 48 hours. Plasma lidocaine levels decreased dramatically at 60 hours post-application. Low plasma lidocaine concentrations remained for 6 hours after patch removal. No clinically significant side effects were noted.  相似文献   

Exploratory study of Mycoplasma suis (Eperythrozoon suis) on four commercial pig farms in southern Brazil     

Guimaraes AM  Biondo AW  Lara AC  Messick JB 《The Veterinary record》2007,160(2):50-53

Mycoplasma suis (Eperythrozoon suis) was detected by PCR and Southern blot in 186 pigs (121 sows, 61 piglets and four boars) on four farms in southern Brazil. DNA was extracted from blood samples and a 16S rRNA gene fragment of M suis was amplified by PCR; Southern blot analysis was then performed on all the samples. Twenty-two of the sows (18.2 per cent) were positive by PCR, and 40 (33.1 per cent) were positive by Southern blot; only one piglet and one boar were positive. The packed cell volume and total plasma protein of the pigs and their PCR and Southern blot results were not significantly different on the four farms, but higher proportions of the pigs were positive by Southern blot than by PCR (P<0.05). The packed cell volume and total plasma protein concentrations of the M suis positive and negative sows were not significantly different.  相似文献   

Spinal cord injury resulting from incorrect microchip placement in a cat     

Platt S  Wieczorek L  Dennis R  De Stefani A 《Journal of Feline Medicine and Surgery》2007,9(2):157-160

A 2-year-old, male neutered domestic shorthair cat was presented for investigation of an acute onset of tetraparesis immediately following the implantation of a pet identification microchip. A left-sided C6-T2 spinal segment localisation was suspected from the neurological examination, with spinal cord trauma being the primary differential diagnosis. Myelography demonstrated obliteration of the contrast columns by the microchip at the C5-C6 intervertebral disc space. A dorsal laminectomy was undertaken and the microchip was successfully removed. Eleven months after the surgery, the cat was able to weight bear in all limbs but with mild residual paresis in the left thoracic limb.  相似文献   

In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation     

Mickael Lafond  Bernard Bouza  Sandrine Eyrichine  Friedrich Rouffineau  Luc Saulnier  Thierry Giardina  Estelle Bonnin  Aurélie Preynat 《畜牧与生物技术杂志(英文版)》2015,6(1)

Background

The filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks to its ability to produce a pool of CAZymes and particularly endo-β(1,4)-xylanases. In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.

Results

Polysaccharides digestion was followed during 6 h along the digestive tract using the TNO gastrointestinal model-1, to mimic monogastric metabolism. Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn. For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

Conclusions

The amounts of arabinose and xylose into the dialysates and ileal deliveries increased consequently mainly for Caphorn, suggesting that XynD and XynB supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy.  相似文献   

Flow cytometric characterization of Peyer's patch and cecal tonsil T lymphocytes in laying hens following challenge with Salmonella enterica serovar Enteritidis     

Peter S. Holt  Lara E. Vaughn  Richard K. Gast 《Veterinary immunology and immunopathology》2010,133(2-4):276-281

Two trials were conducted to determine T cell changes in Peyer's patches (PP) and cecal tonsils (CT) of specific-pathogen-free Single-Comb White Leghorn hens challenged with Salmonella enterica serovar Enteritidis (SE). Each week, crop lavage samples were obtained from 4 or 3 hens in Trials 1 and 2, respectively. These birds were then sacrificed and their intestinal tracts excised. The crop sample and contents of one cecum from each hen were cultured for the presence of SE. Cells were purified from proximal and distal PP along with both CT and then aliquots of cells were incubated with antibodies to CD4, CD8, and the three T cell receptors (TCR). The T subsets were identified via flow cytometric analysis. Crop and cecal samples were 100% culture positive for SE at week 1 post challenge and a percentage of samples remained positive throughout the study. Some differences in TCR subsets between or within tissues were observed at various times relative to SE challenge but over-all the subsets remained similar during the study. The predominant TCR was TCR2 (vβ1) followed by TCR3 (vβ2). Low numbers of TCR1 (γδ) cells were observed. CD4/CD8 ratios increased in the PP and CT tissues by week 1 post challenge and the ratio elevation persisted throughout the experiment. These results indicate that T cell populations are comparable between PP and CT and enteric SE infection can affect the cellular dynamics of these lymphoid tissues.  相似文献   

Cutaneous progressive angiomatosis on the muzzle of a dog,treated by laser photocoagulation therapy     

Lara Olivieri  Giordano Nardini  Graziano Pengo  Francesca Abramo 《Veterinary dermatology》2010,21(5):517-521

A 10‐year old, female, cocker spaniel was presented with a bleeding lesion on the muzzle and nasal planum observed initially as a small raised nodule at 1 year of age. This became clinically more pronounced at 5 years of age, progressively enlarged thereafter and was diagnosed initially as a vascular tumour. On clinical examination, multiple confluent nodules of variable diameter (0.5–1.5 cm) deformed the outline of the nose. Histological examination revealed a progressive angiomatosis with multifocal infiltrative growth of increased dermal vascularization with different sized and type of anastomosing vessels lined by a monolayer of reactive endothelium, often arranged as papillary projections into the lumina. Partial nosectomy, the first therapeutic approach, was unsuccessful and the lesion relapsed within 6 months. However, subsequent laser photocoagulation therapy provided a good cosmetic outcome, no relapse 1 year later, and appeared to provide an effective alternative to ablation surgery.  相似文献