Performance of <Emphasis Type="Italic">Coffea arabica</Emphasis> F1 hybrids in agroforestry and full-sun cropping systems in comparison with American pure line cultivars     

B. Bertrand  E. Alpizar  L. Lara  R. SantaCreo  M. Hidalgo  J. M. Quijano  C. Montagnon  F. Georget  H. Etienne 《Euphytica》2011,181(2):147-158

Coffea arabica F1 hybrids derived from crosses between wild Sudan-Ethiopian and American cultivars and propagated by somatic embryogenesis have been obtained in Central America. These new hybrids considerably enhanced the genetic diversity of coffee in the region. We conducted 15 trials to assess whether using hybrids represents substantial genetic progress in terms of productivity in agroforestry and full-sun cropping systems. The new germplasm was grown in the same conditions as the best American cultivar (homozygous pure lines). The results showed that yields of hybrids were earlier and superior to those of American cultivars. The hybrids were also more stable than the American cultivars in all environments. In the agroforestry system, the mean yield of hybrids was 58% higher than that of the American cultivars, while the mean yield of hybrids in the full-sun system was 34% higher. Coffee-based agroforestry systems (AS) are considered effective in protecting the environment in the volcanic cordilleras of Central America. We found that introducing hybrids in coffee-based AS can considerably increase productivity. This finding could be a convincing argument to encourage coffee growers who have adopted the full-sun cropping system to return to agroforestry cropping systems. Finally, the conditions for large-scale dissemination of those new hybrids—which represent a major innovation for C. arabica cropping—was analysed.  相似文献   

The Selaginella genome identifies genetic changes associated with the evolution of vascular plants     

Banks JA  Nishiyama T  Hasebe M  Bowman JL  Gribskov M  dePamphilis C  Albert VA  Aono N  Aoyama T  Ambrose BA  Ashton NW  Axtell MJ  Barker E  Barker MS  Bennetzen JL  Bonawitz ND  Chapple C  Cheng C  Correa LG  Dacre M  DeBarry J  Dreyer I  Elias M  Engstrom EM  Estelle M  Feng L  Finet C  Floyd SK  Frommer WB  Fujita T  Gramzow L  Gutensohn M  Harholt J  Hattori M  Heyl A  Hirai T  Hiwatashi Y  Ishikawa M  Iwata M  Karol KG  Koehler B  Kolukisaoglu U  Kubo M  Kurata T  Lalonde S  Li K  Li Y  Litt A  Lyons E  Manning G  Maruyama T  Michael TP 《Science (New York, N.Y.)》2011,332(6032):960-963

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Variation in the levels of the different xylanase inhibitors in grain and flour of 20 French wheat cultivars     

Estelle Bonnin  Stphanie Daviet  Kurt Gebruers  Jan A. Delcour  Andrew Goldson  Nathalie Juge  Luc Saulnier 《Journal of Cereal Science》2005,41(3):375-379

A xylanase from Aspergillus aculeatus (glycoside hydrolase family 10), uninhibited by TAXI, and a xylanase from Bacillus subtilis (family 11), uninhibited by XIP-I, were selected to quantify the respective apparent levels of XIP-I and TAXI inhibitors, in flours and grains of 20 wheat varieties. The apparent TAXI amount ranged from 0.05 to 0.19 mg/g in flour (mean: 0.11 mg/g) and from 0.07 to 0.2 mg/g in grain (mean: 0.14 mg/g). The range observed for XIP-I was 0.12–0.6 mg/g in flour (mean 0.32 mg/g), and 0.21–0.56 mg/g in grain (mean: 0.41 mg/g). The inhibition profile of the xylanase from A. aculeatus by a crude inhibitor preparation suggested the presence of an additional component in wheat flour, responsible for an increase in inhibition.  相似文献   

Effects of Lime‐Cooking on Carotenoids Present in Masa and Tortillas Produced from Different Types of Maize     

Janet A. Gutirrez‐Uribe  Carlos Rojas‐García  Silverio García‐Lara  Sergio O. Serna‐Saldivar 《Cereal Chemistry》2014,91(5):508-512

Yellow and white maize kernels, masas, tortillas, and nejayote solids were analyzed in terms of lutein, zeaxanthin, cryptoxanthin, β‐carotene, and lipophilic antioxidant (AOX) capacity. The germplasm analyzed included two normal yellow maize, two high‐carotenoid genotypes, and one white for comparison purposes. In general, the yellow maize required 34% more lime‐cooking time compared with the white counterpart. Lime‐cooking significantly changed the extractability of carotenoids in masa and tortillas. No carotenoids were detected in the steepwater or nejayote. The lipophilic AOX activity increased 280‐fold from kernel to masa, but only 70% was retained in the baked tortillas. When masa was baked into tortillas, less than 10% of the carotenoids were retained because of the high temperatures used during baking. Interestingly, tortillas made with the maize kernels with the highest carotenoid content did not have the highest amount of these phytochemicals. Therefore, maize varieties should be evaluated based on the carotenoid content in finished food products instead of the amounts originally found in raw kernels.  相似文献   

Quantification of individual phosphorus species in sediment: a sequential conversion and extraction method     

J. F. Oxmann  Q. H. Pham  R. J. Lara 《European Journal of Soil Science》2008,59(6):1177-1190

Common sequential phosphorus (P) extraction methods are not specific to particular chemical species and have several limitations. This work presents the first chemical method for quantification of individual mineral and sorbed P species. It was developed by combining a conversion technique with a sequential extraction procedure. Mangrove sediments with different characteristics were incubated in pH‐adjusted 0.01 M CaCl₂ with and without reference material additions of octacalcium phosphate (Ca₈H₂(PO₄)₆·5H₂O; OCP), hydroxyapatite (Ca₅(PO₄)₃OH), strengite (FePO₄·2H₂O) or variscite (AlPO₄·2H₂O). The changes in soluble phosphate concentration were measured in the supernatant solution, while pH‐induced variations in P composition were determined by subsequent sequential extraction of the sediments. Dissolved phosphate concentration was controlled by adsorption below pH 7.8. Above this pH, soluble phosphate concentration was governed by OCP, which was qualitatively determined by plotting the experimental values of pH + pH₂PO₄ and pH – 0.5 pCa on a solubility diagram including the isotherms of known crystalline phosphate compounds. In contrast to the often‐predicted slow dissolution rate of crystalline phosphates in soils or sediments, drastic changes in P composition by dissolution, precipitation and adsorption processes were detected after 7 days. These were mainly not observed indirectly by changes in dissolved phosphate through adsorption effects, but were determined quantitatively by subsequent sequential extraction, thus enabling the quantification of individual species. Evaluation of the method was performed by standard addition experiments. Besides P species quantification, the method provides the means for other applications, such as the determination of P mineral dissolution kinetics in soils and sediments, the prediction of P composition in changing environmental settings and the refinement of theoretical models of phosphate solubility in soil and sedimentary environments.  相似文献   

Long-term storage of Pink Lady apples modifies volatile-involved enzyme activities: consequences on production of volatile esters     

Villatoro C  Echeverría G  Graell J  López ML  Lara I 《Journal of agricultural and food chemistry》2008,56(19):9166-9174

Pink Lady apples were harvested at commercial maturity and stored at 1 degrees C and 92% relative humidity under either air or controlled atmosphere conditions (2 kPa O 2:2 kPa CO 2 and 1 kPa O 2:1 kPa CO 2) for 27 weeks. Data on the emission of volatile compounds and on the activity of some related enzymes in both skin and flesh tissues were obtained during subsequent shelf life at 20 degrees C. Major effects of storage atmosphere and poststorage period were observed on the emission of volatile esters and their precursors. Changes in the production of volatile esters were partly due to alterations in the activity of alcohol o-acyltransferase, but the specific esters emitted by fruit after storage also resulted largely from modifications in the supply of the corresponding substrates. Samples stored under air were characterized by higher availability of acetaldehyde, whereas those stored under CA showed enhanced emission of the alcohol precursors ethanol and 1-hexanol (2 kPa O 2) and 1-butanol (1 kPa O 2), with accordingly higher production of ethyl, hexyl, and butyl esters. Multivariate analysis revealed that a large part of the observed differences in precursor availability arose from modifications in the activity of the enzymes considered. Higher pyruvate decarboxylase activity in air-stored fruit possibly accounted for higher acetaldehyde levels in these samples, while storage under 1 kPa O 2 led to significantly decreased lipoxygenase activity and thus to lessened production of 1-hexanol and hexyl esters. Low acetaldehyde availability together with enhanced hydroperoxide lyase and alcohol dehydrogenase levels in these fruits are suggested to have led to higher emission of 1-butanol and butyl esters.  相似文献   

Regeneration of volatile compounds in Fuji apples following ultra low oxygen atmosphere storage and its effect on sensory acceptability     

Altisent R  Graell J  Lara I  López L  Echeverría G 《Journal of agricultural and food chemistry》2008,56(18):8490-8497

The aim of this work was to assess whether extra time spent under AIR conditions after storage in an ultra low oxygen (ULO) atmosphere could allow the regeneration of volatile compound emission without negatively affecting quality parameters and the consumer acceptability of Fuji apples. Fruits were stored for 19 and 30 weeks at 1 degrees C and 92% RH under ULO atmosphere conditions (1 kPa O 2:1 kPa CO 2) or under ULO conditions followed by different periods (2 and 4 weeks) in cold AIR atmosphere (ULO + 2w or ULO + 4w, respectively). Standard quality and emission of volatile compounds were analyzed after storage plus 1 and 7 days at 20 degrees C. Sensory attributes and acceptability were also determined after 7 days at 20 degrees C. The extra period of 30 weeks in an AIR atmosphere after ULO storage resulted in an increase in the concentration of the compounds that most contribute to the flavor of Fuji apples. These fruits were relatively well accepted by consumers despite a slight decline in firmness and acidity.  相似文献   

Shelf life modeling of photosensitive food: the case of colored beverages     

Manzocco L  Kravina G  Calligaris S  Nicoli MC 《Journal of agricultural and food chemistry》2008,56(13):5158-5164

A new approach to shelf life modeling of photosensitive foods was developed taking into consideration the example of a saffron-containing yellow beverage highly prone to oxidative photobleaching. The beverage was exposed to different light levels at increasing temperatures. During exposure, samples were analyzed for bleaching rate, pigment content, and pigment degradation products. The results obtained clearly showed that shelf life testing of light-sensitive foods must take into proper account the effect of light. In addition, for these foods, shelf life models based on the sole accelerating effect of temperature may be misleading. By contrast, the concomitant exploitation of the accelerating effects of both light and temperature was used to develop and validate a simple model correctly predicting the shelf life of the beverage under actual storage conditions. The methodology proposed may allow solving of the difficult task of predicting shelf life of photosensitive foods usually marketed in the presence of light.  相似文献   

Can malignant and inflammatory pleural effusions in dogs be distinguished using computed tomography?          下载免费PDF全文

Thom C. Watton  Ana Lara‐Garcia  Christopher R. Lamb 《Veterinary radiology & ultrasound》2017,58(5):535-541

Computed tomography (CT) is the primary imaging modality used to investigate human patients with suspected malignant or inflammatory pleural effusion, but there is a lack of information about the clinical use of this test in dogs. To identify CT signs that could be used to distinguish pleural malignant neoplasia from pleuritis, a retrospective case‐control study was done based on dogs that had pleural effusion, pre‐ and postcontrast thoracic CT images, and cytological or histopathological diagnosis of malignant or inflammatory pleural effusion. There were 20 dogs with malignant pleural effusion (13 mesothelioma, 6 carcinoma; 1 lymphoma), and 32 dogs with pleuritis (18 pyothorax; 14 chylothorax). Compared to dogs with pleuritis, dogs with malignant pleural effusions were significantly older (median 8.5 years vs. 4.9 years, P = 0.001), more frequently had CT signs of pleural thickening (65% vs.34%, P = 0.05), tended to have thickening of the parietal pleura only (45% vs. 3%, P = 0.002) and had more marked pleural thickening (median 3 mm vs. 0 mm, P = 0.03). Computed tomography signs of thoracic wall invasion were observed only in dogs with malignant pleural effusions (P = 0.05). There were no significant differences in pleural fluid volume, distribution or attenuation, degree of pleural contrast accumulation, amount of pannus, or prevalence of mediastinal adenopathy. Although there was considerable overlap in findings in dogs with malignant pleural effusion and pleuritis, marked thickening affecting the parietal pleural alone and signs of thoracic wall invasion on CT support diagnosis of pleural malignant neoplasia, and may help prioritize further diagnostic testing.  相似文献   

Retrospective evaluation of COX‐2 expression,histological and clinical factors as prognostic indicators in dogs with renal cell carcinomas undergoing nephrectomy          下载免费PDF全文

S. Carvalho  A. L. Stoll  S. L. Priestnall  A. Suarez‐Bonnet  K. Rassnick  S. Lynch  I. Schoepper  G. Romanelli  P. Buracco  M. Atherton  E. M. de Merlo  A. Lara‐Garcia 《Veterinary and comparative oncology》2017,15(4):1280-1294

Limited veterinary literature is available regarding prognostic markers for canine renal cell carcinoma (CRCC). We retrospectively evaluated COX‐2 expression, histological and clinical features associated with prognosis of CRCC. Sixty‐four cases post‐nephrectomy were included, 54 had histopathological assessment and 30 had COX‐2 immunostaining performed. Eight dogs (13%) had metastatic disease at initial diagnosis. Twenty‐seven dogs (42%) received adjuvant therapy after nephrectomy. On univariate analysis, COX‐2 expression, mitotic index (MI), histologic type, vascular invasion, neoplastic invasiveness and metastasis at diagnosis were significantly associated with overall median survival time (MST). COX‐2 score (COX‐2 score > 3 MST 420 days versus 1176 days if COX‐2 score <3; P = 0.011) and MI (MI > 30 MST 120 days versus 540 days for MI < 30; P = 0.003) were the only variables associated with CRCC outcome on multivariate analysis. The addition of MI and COX‐2 immunostaining to standard histopathological evaluation would help predicting outcome in CRCC patients.  相似文献