Isolation of Mycoplasma mycoides from outbreaks of caprine mycoplasmosis in Brazil     

E R Nascimento  M da G Nascimento  E A Freundt  H Andersen 《The British veterinary journal》1986,142(3):246-257

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Flow cytometric assay in peripheral blood of dogs--reference values for leukocytes from Brazilian beagles     

Fujiwara RT  Bueno LL  Vale AM  Franca-Silva JC  da Costa RT  Quetz Jda S  Machado-Coelho GL  Reis AB  Martins Filho OA  Genaro O  Nascimento E  Mayrink W 《Polish journal of veterinary sciences》2005,8(1):17-22

Use of domestic reference values in the flow cytometry analysis is known to improve its accuracy by integrating local variations as gender, race and age. Up to date application of flow cytometry in veterinary medicine has been limited to describe the percentual values just for peripheral lymphocytes subsets of blood. We now report establishment of reference values for a wide range of proportional and absolute numbers of peripheral blood leukocytes, including T cells subsets, B cells, monocytes and eosinophils, applicable to the healthy population of Beagles in Brazil and other regions with similar demographic characteristics. Normal reference values were also established to estimate the gender-related differences. This information will provide clinical aid in the evaluation of immunologic status as well as standard values for experimental animals of dogs from Brazil and other similar regions.  相似文献   

Detection of the enterotoxins A, B, and C genes in Staphylococcus aureus from goat and bovine mastitis in Brazilian dairy herds   总被引：2，自引：0，他引：2  

da Silva ER  do Carmo LS  da Silva N 《Veterinary microbiology》2005,106(1-2):103-107

To determine the distribution of genes that encode enterotoxins A, B and C, 36 strains of Staphylococcus aureus isolated from goat mastitis and 64 isolated from bovine mastitis were analyzed by Multiplex PCR. Of the total strains studied, 37 (37%) were detected to have some of the SEs genes. From the bovine mastitis strains, 4 (6.3%) co-amplified the sea and seb genes and 2 (3.1%) were positive for the sec gene. From the goat mastitis strains, 31 (86%) tested positive to the Multiplex, and the sec gene was detected in all of them. The production of SE was detected in all strains harboring the corresponding gene. The results demonstrated that S. aureus isolated from goat mastitis had a higher enterotoxigenic potential than those isolated from bovine mastitis. Additionally, the presence of the sec gene in the majority of goat mastitis strains suggests a possible involvement of SEC in goat mastitis pathogenesis.  相似文献   

Observations on helminth infections of free-living and captive rheas (Rhea americana) in Brazil     

Zettermann CD  Nascimento AA  Tebaldi JA  Szabó MJ 《Veterinary parasitology》2005,129(1-2):169-172

The present work describes helminth infection of eight free-living and 12 captive rheas (Rhea americana) from, respectively, Pantanal of Mato Grosso do Sul State, and Jaboticabal, Sao Paulo State, Brazil. Captive birds were young and had a high mortality rate, while free-living birds were adult and apparently healthy. Infections were evaluated by post-mortem examination of internal organs and recovery of helminths using standard parasitological procedures. Seven species of nematodes (Sicarius uncinipenis, Torquatoides crotophaga, Deletrocephalus dimidiatus, D. cesarpintoi, Paradeletrocephalus minor, Capillaria venteli and Dicheilonema rheae) and two species of cestodes (Houttuynia struthionis and Chapmania tauricolis) were identified. P. minor, which inhabits the large intestine, was the most common helminth in free-living birds (63.9%). In captive rheas, a mean parasitic load of 173 helminths per host was found. The gizzard of these birds was the most parasitized organ and S. uncinipenis was most common (92.5%). Parasitism of free-living and captive birds and associated pathology are discussed.  相似文献   

Cervical diskospondylitis in a calf: clinical, radiographic, and necroscopy findings          下载免费PDF全文

Paiva Della Libera AM  Leal ML  Gregory L  Rodrigues da Silva DY  Unruh SM  Birgel Júnior EH  Benesi FJ 《The Canadian veterinary journal. La revue veterinaire canadienne》2004,45(8):700-701

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Effects of probiotics and maternal vaccination on Salmonella enteritidis infection in broiler chicks     

Avila LA  Nascimento VP  Salle CT  Moraes HL 《Avian diseases》2006,50(4):608-612

The effects of probiotics and maternal vaccination with an inactivated Salmonella Enteritidis (SE) vaccine on day-old chicks challenged with SE were evaluated. A 2 X 3 factorial arrangement was used (with or without probiotics; breeders nonvaccinated, vaccinated intramuscularly, or vaccinated intraperitoneally). Three trials were conducted in isolation cabinets and SE challenge was different between trials. The number of SE organisms per chick and the time interval between housing and introduction of seeder birds (hereafter called challenge) were 1.6 X 10(8) and 1 hr (Trial I), 1.8 X 10(6) and 12 hr (Trial II), and 1.2 X 10(4) and 24 hr (Trial III). SE recovery was assessed in ceca and liver at 3, 5, and 7 days postchallenge, and the number of colony-forming units (CFU) in ceca was evaluated at 5 and 7 days postchallenge. The number of SE (log CFU) in the ceca reduced 0.56 log (from 7.59 to 7.03) and 1.45 log (7.62 to 6.17) because of the treatment with probiotics in Trials II and III, respectively. The greater reduction in Trial III indicates the importance of the early use of probiotics on the prevention of SE infection. Treatment with probiotics resulted in a smaller number of SE-positive livers after 5 days postchallenge on Trial III. Although there was no significant effect of maternal vaccination on the number of SE CFU in the ceca, a significant effect of maternal vaccination on the SE CFU was observed in the liver, but not in the ceca at 5 days after challenge.  相似文献   

Molecular cloning and characterization of sea bass (Dicentrarchus labrax L.) CD8alpha     

Pinto RD  Nascimento DS  Vale Ad  Santos NM 《Veterinary immunology and immunopathology》2006,110(1-2):169-177

In this work, the gene and cDNA of the sea bass CD8alpha have been isolated and characterized. The coding sequence has an ORF of 666 bp. It retains the Ig motif that interacts with MHC and the two cysteines responsible for an intra-chain disulfide bridge. The hinge region contains the two essential cysteines involved in dimerization. The transmembrane region is well conserved in all analysed sequences. Similar to other teleosts, the cytoplasmic region lacks the consensus p56(lck) motif common in higher vertebrates. Analysis of the expression pattern using RT-PCR shows the highest expression in the thymus. Like in the human gene, the sea bass CD8alpha genomic structure is organized into six exons, which roughly correspond to separate functional domains of the protein. Southern blotting shows that CD8alpha exists as a single copy gene. Together, these results support the concept that the basic structure of CD8alpha has been maintained through evolution.  相似文献   

Expression profiling of genes involved in the biotrophic colonisation of Coffea arabica leaves by Hemileia vastatrix     

Ana Vieira  Pedro Talhinhas  Andreia Loureiro  Johannes Thürich  Sébastien Duplessis  Diana Fernandez  Maria do Céu Silva  Octávio S. Paulo  Helena Gil Azinheira 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(1):261-277

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Critical disease components of common bacterial blight to effectively evaluate resistant genotypes of snap bean     

Roberto dos Santos Trindade  Rosana Rodrigues  Ant?nio Teixeira do Amaral Júnior  Leandro Sim?es Azeredo Gon?alves  Rogério Figueiredo Daher  Cláudia Pombo Sudré 《Journal of General Plant Pathology》2012,78(3):201-206

Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli (Xap) is the main bacterial disease of snap bean. The present work aimed to select snap bean genotypes that are resistant to CBB based on three components of resistance: area under the disease progress curve, latent period, and lesion diameter on pods (DL). A completely randomized two-factor factorial design with six replications was used to evaluate leaves and pods of 14 snap bean and three common bean genotypes (PI 207262, BAC-6 and A-794) with high resistance to CBB. Two Xap isolates, 1394-98 and 775-90, were used to inoculate leaves and pods. Data were analyzed using nonparametric statistics. Significant differences were observed among the evaluated genotypes for all of the components of resistance, except for DL. The snap bean UENF 1482 demonstrated good performance in two disease resistance components. For this reason, this genotype can be recommended for use in breeding programs that aim to generate snap bean genotypes resistant to Xanthomonas axonopodis pv. phaseoli.  相似文献   

Downgrading of heavy broiler chicken carcasses due to myodegeneration of the anterior latissimus dorsi: pathologic and epidemiologic studies     

Zimermann FC  Fallavena LC  Salle CT  Moraes HL  Soncini RA  Barreta MH  Nascimento VP 《Avian diseases》2012,56(2):418-421

Recently, in some Brazilian poultry companies, a dorsal cranial muscular lesion has been increasingly detected in broilers, causing heavy economic losses due to carcass downgrading. The observed gross lesions located in the anterior latissimus dorsi (ALD) muscle are characterized by yellowish discoloration of the skin and swelling on the dorsal cranial region of that muscle. When the ALD muscle is cut, subcutaneous edema, muscular superficial hemorrhage, pallor, adherence, and increased thickness and density are observed. Microscopically, findings indicate degenerative and polyphasic features, variation in fiber size and splitting, presence of hyaline, necrotic and regenerative myofibers, extensive fibrosis, and adipose tissue with lymphohistiocytic infiltration in all ALD muscles affected. The etiology of the lesion is unknown, and no detailed report was found in literature. The highest frequency of carcass downgrading due to this lesion was found in the heaviest and the oldest males of high-yield broiler strains (P < 0.01). This study is the first to describe the pathologic and some epidemiologic aspects of this new myopathy.  相似文献