Infection of human enterovirus 71 in immunosuppressed rhesus monkeys     

WANG Jing-jing  ZHANG Xue-mei  ZHAO Hong-ling  DONG Cheng-hong  PU Jing  LIAO Yun  WANG Li-chun  NA Rui-xiong  LIU Long-ding  ZHANG Ying  YANG Li-xian  WANG Qing-ling  SHEN Dong  WANG Xi  LI Qi-han 《园艺学报》2011,27(7):1249-1256

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.  相似文献   

磁化水在农作物上应用的研究进展     

赵黎明  李明  郑殿峰  顾春梅  那永光  解保胜 《黑龙江农业科学》2014,(10):158-160

针对磁化水对农业生产的影响,简述了国内外关于磁化水应用的研究动态和发展趋势,介绍了磁化水在促进作物种子萌发、增强抗逆性以及提高产量和改善品质方面的研究进展。  相似文献   

Systematic review of the treatment options for pericardial effusions in dogs     

Logan M. Scheuermann  Wanda J. Gordon‐Evans  Andr J. Nault 《Veterinary surgery : VS》2021,50(1):20-28

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我国和欧盟兽药质量监督抽检工作机制对比研究     

张秀英  吴好庭  张骊  李美花  李宁  李琰  娜琳  王小慈  张存帅 《中国兽药杂志》2021,55(1):64-69

为更好地完善我国兽药质量监督抽检计划,通过对欧盟药品抽检管理工作机制的研究,本文分析了欧盟和我国兽药监督抽检管理方式的不同,从抽检责任部门、抽检目的、抽检计划内容、抽检计划制定流程、抽检品种的遴选和检测参数的确定等6方面进行了对比分析,探讨我国兽药质量监督抽检工作的管理中可以借鉴的方面。  相似文献   

兴安盟地区旱作条件下11个苜蓿品种生产性能和饲用价值比较     

那亚  姜永成  包健  凌红波  初文凯  董艳伟  陶雅 《饲料研究》2021,(2):99-104

为筛选兴安盟地区旱作条件下适宜引进栽培的优良苜蓿品种,在兴安盟乌兰浩特市呼和马场种植11个苜蓿(Medicago sativa)品种,通过连续2年观测参试品种的越冬率、株高、产量、茎叶比及营养成分等指标,对旱作条件下11个苜蓿品种的生产性能和饲用价值进行灰色关联分析。结果表明:图牧2号、敖汉苜蓿和呼伦贝尔杂花苜蓿两年的平均越冬率较高;SK3010、呼伦贝尔杂花苜蓿和敖汉苜蓿在株高、生长速度和干草产量方面表现较好;图牧2号的茎叶比均最大,其次是呼伦贝尔杂花苜蓿、SK3010和敖汉苜蓿;WL168HQ、斯普雷德和呼伦贝尔杂花苜蓿两年的粗蛋白质含量均高于18%;巨能2和斯贝德的粗纤维含量较高;WL168HQ、呼伦贝尔杂花苜蓿、WL343HQ、SK3010和WL319HQ的粗灰分含量均高于10%。对11个苜蓿品种的越冬率、产量特性和饲用品质进行灰色关联分析表明,呼伦贝尔杂花苜蓿的综合表现最优,适合在该地区种植推广。  相似文献   

蒙古口蘑担孢子萌发及初生菌丝生物学特性研究   总被引：1，自引：0，他引：1  

刘晓婷  郭九峰  王淑妍  李亚娇  袁丽丽  那日 《北方园艺》2016,(16):136-141

以蒙古口蘑(Tricholoma mongolicum)为试材,采用光学显微镜观察担孢子及菌丝体形态的方法,研究了不同培养基对蒙古口蘑担孢子萌发及初生菌丝生长情况的影响。结果表明:蒙古口蘑担孢子呈肾形或椭圆形,大小约为(4~6)μm×(6~9)μm;担孢子在培养基上萌发出肉眼可见的微小菌落至少需要1周的时间;MS培养基中添加1.5g·L~(-1)酵母粉和1.5g·L~(-1)酸水解酪蛋白,对担孢子萌发有明显的促进作用;根据初生菌丝菌落生长速度和菌落形态等有关特征,发现有5种明显不同类型的初生菌丝,分别为菌丝绒毛型、菌丝贴生型、菌丝稀薄型、成簇生长型、缓慢生长型。另外,采用rDNA-ITS方法、PCR产物测序及NCBI-Blast比对,证明试验材料为蒙古口蘑。该试验提供了一种食用菌菌丝体直接PCR的方法,为进行有关食用菌DNA分子方面研究提供了便利;该研究结果可为蒙古口蘑担孢子交配型研究提供依据,为珍稀食用菌研究积累资料。  相似文献   

The evolving means of protecting horses against West Nile infection through immunisation     

Traub-Dargatz JL  Cordes T  Evans MB 《Equine veterinary journal》2007,39(6):484-485

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Genetic and non-genetic factors influencing KLH binding natural antibodies and specific antibody response to Newcastle disease in Kenyan chicken populations     

Sophie Miyumo  Chrilukovian B. Wasike  Evans D. Ilatsia  Jörn Bennewitz  Mizeck G. Chagunda 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2023,140(1):106-120

This study aimed at investigating the influence of genetic and non-genetic factors on immune traits to inform on possibilities of genetic improvement of disease resistance traits in local chicken of Kenya. Immune traits such as natural and specific antibodies are considered suitable indicators of an individual's health status and consequently, used as indicator traits of disease resistance. In this study, natural antibodies binding to Keyhole Limpet Hemocyanin (KLH-NAbs) was used to measure general disease resistance. Specific antibodies binding to Newcastle disease virus (NDV-IgG) post vaccination was used to measure specific disease resistance. Titers of KLH-NAbs isotypes (KLH-IgM, KLH-IgG and KLH-IgA) and NDV-IgG were measured in 1,540 chickens of different ages ranging from 12 to 56 weeks. A general linear model was fitted to determine the effect of sex, generation, population type, phylogenetic cluster, line, genotype and age on the antibody traits. A multivariate animal mixed model was fitted to estimate heritability and genetic correlations among the antibody traits. The model constituted of non-genetic factors found to have a significant influence on the antibody traits as fixed effects, and animal and residual effects as random variables. Overall mean (±SE) concentration levels for KLH-IgM, KLH-IgG, KLH-IgA and NDV-IgG were 10.33 ± 0.04, 9.08 ± 0.02, 6.00 ± 0.02 and 10.12 ± 0.03, respectively. Sex, generation and age (linear covariate) significantly (p < 0.05) influenced variation across all the antibody traits. Genotype effects (p < 0.05) were present in all antibody traits, apart from KLH-IgA. Interaction between generation and line was significant (p < 0.05) in KLH-IgM and NDV-IgG while nesting phylogenetic cluster within population significantly (p < 0.05) influenced all antibody traits, apart from KLH-IgA. Heritability estimates for KLH-IgM, KLH-IgG, KLH-IgA and NDV-IgG were 0.28 ± 0.08, 0.14 ± 0.06, 0.07 ± 0.04 and 0.31 ± 0.06, respectively. There were positive genetic correlations (0.40–0.61) among the KLH-NAbs while negative genetic correlations (−0.26 to −0.98) were observed between the KLH-NAbs and NDV-IgG. Results from this study indicate that non-genetic effects due to biological and environmental factors influence natural and specific antibodies and should be accounted for to reduce bias and improve accuracy when evaluating the traits. Subsequently, the moderate heritability estimates in KLH-IgM and NDV-IgG suggest selection possibilities for genetic improvement of general and specific immunity, respectively, and consequently disease resistance. However, the negative correlations between KLH-NAbs and NDV-IgG indicate the need to consider a suitable approach that can optimally combine both traits in a multiple trait selection strategies.  相似文献   

残膜回收工艺和收膜机构   总被引：2，自引：0，他引：2  

张惠友  侯书林  那明君  董欣  李元强  杨晓丽  白圣男 《农机化研究》2004,(6):72-73

介绍了田间残膜的特性及机械化收膜工艺、残膜同收机中常用收膜机构的种类及工作特性，为残膜回收机的研究设计及主要工作部件结构的优化组合提供参考。  相似文献   

仿形雕刻机的设计     

李元强  那明君  杨晓丽  张惠友 《农机化研究》2004,(4):87-88

木雕制品是对木材经过艺术构思后进行雕刻加工的一种工艺制品，可以用来装饰居室、殿堂、建筑，给人带来美及艺术的享受。随着人们生活水平的提高，该制品的需求量越来越大，产业前景非常好。为此，介绍了一种新型仿形雕刻机的结构和工作原理，利用该装备可以实现对木雕制品及木制品的仿形加工。  相似文献