Vaccination of gnotobiotic primary specific pathogen-free pigs against Haemophilus parasuis.   总被引：6，自引：2，他引：4       下载免费PDF全文

O P Miniats  N L Smart    E Ewert 《Canadian journal of veterinary research》1991,55(1):33-36

Three trials were conducted to establish if young primary specific pathogen free (SPF) pigs could be protected from Glasser's disease by vaccination. Three age groups of cesarean-derived isolator-reared gnotobiotic pigs were vaccinated twice at 4 and 6, 3 and 5, and 2 and 4 wk of age respectively with a formalin killed aluminum hydroxide adsorbed bacterin prepared from three strains of Haemophilus parasuis isolated from Ontario pigs affected with Glasser's disease. When challenged two weeks later with the homologous strains of virulent bacteria, all the vaccinated pigs remained healthy, while 17/18 nonvaccinated pigs became severely sick or died between three and seven days postchallenge. The one surviving nonimmunized pig was retarded in growth. All of the nonimmunized pigs had visible lesions of polyserositis, the most common being polyarthritis (14/18). Other lesions were fibrinous meningitis, pericarditis, pleurisy and/or peritonitis. Two of the pigs died with a septicemia. Haemophilus parasuis was isolated from 15/18 nonimmunized pigs, usually from several of the affected sites. The organisms were not isolated from the immunized pigs, nor from the surviving nonimmunized pig. Attempts to detect the presence of specific antibodies against the H. parasuis strains in the sera of the immunized or exposed pigs by the passive hemagglutination test or by enzyme linked immunoassay were unsuccessful. The results of this work indicate that primary SPF pigs can be protected from Glasser's disease by vaccination as early as 2 and 4 wk of age. The nature of this protective mechanism was not established in this study.  相似文献   

Classical swine fever in Victorian domestic pigs: evidence of disease freedom     

JC Hunnam  KM Moore  P Daniel  MA Stevenson  SE Salmon 《Australian veterinary journal》2019,97(11):447-451

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Dachshund bitch with severe uterine adhesions and intramural uterine foreign material as an incidental ovariohysterectomy finding          下载免费PDF全文

KM Watson  NU Horadagoda  SA Piripi 《Australian veterinary journal》2016,94(1-2):24-26

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Acute pasteurellosis in fallow deer, cattle and pigs in a region of Eastern Germany     

Soike D  Schulze C  Kutzer P  Ewert B  van der Grinten E  Schliephake A  Ewers C  Bethe A  Rau J 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(3-4):122-128

Hemorrhagic septicaemia, an acute disease caused by P multocida capsular type B which is rarely detected in Europe, caused considerable losses in fallow deer, cattle and pigs within a region along the border of the federal states Brandenburg and Saxony-Anhalt in the summer of 2010. Clinical appearances and diagnostic findings are presented and possible triggering influences discussed. Pasteurella multocida capsular type B has not been cultivated from clinically healthy cattle and pigs of the region. Examination of fallow deer and roe deer in the region revealed the presence of singular carriers, which may act as a source of clinical infections.  相似文献   

Changes in body weight and condition of gestating mares     

L.M. Lawrence MS  PhD  J. DiPietro DVM  MS  K. Ewert DVM  MS  D. Parrett MS  PhD  L. Moser MS  D. Powell MS 《Journal of Equine Veterinary Science》1992,12(6):355-358

Changes in body weight, condition scores, and rump fat thickness were observed in 12 mares during an entire gestation period. Mares had ad libitum access to forage (alfalfa hay or haylage) during the study. Total weight gain during gestation averaged 16% of the mares' initial body weight. The majority of weight gain occurred in the second, rather than third trimester of gestation. Only 30% of the total weight gain occurred in the third trimester of gestation. Estimates of body stores (condition score and rump fat thickness) increased during the second trimester of gestation as well. These observations suggest that the effects of pregnancy on the nutritional requirements of mares may not be confined to the last trimester of gestation.  相似文献   

Effect of aflatoxin on the humoral and cell-mediated immune systems of the chicken.   总被引：6，自引：0，他引：6  

J J Giambrone  D L Ewert  R D Wyatt  C S Eidson 《American journal of veterinary research》1978,39(2):305-308

Chickens fed 2.5 microgram of aflatoxin/g of diet from 2 to 4 weeks of age or from hatching to 4 weeks were deficient in cell-mediated immunity, as measured at 4 weeks of age by the graft-versus-host reaction. Delayed-type hypersensitive skin reactions to tuberculin were also reduced in chickens given dietary aflatoxin from hatching to 7 weeks of age. Humoral immunity, as measured by the ability of 4-week-old chicks to produce natural agglutinins to rabbit red blood cells, was not significantly altered by dietary aflatoxin. A significant decrease in concentrations of serum immunoglobulins (Ig) IgG and IgA, but no IgM, however, did occur in chicks given dietary aflatoxin from hatching to 4 weeks or between 2 and 4 weeks of age. Aflatoxin consumption from 0 to 2 weeks of age produced no marked effect on either cell-mediated or humoral immunity in 4-week-old chicks.  相似文献   

Isolation and molecular characterisation of Neospora caninum in cattle in New Zealand     

CM Okeoma  NB Williamson  WE Pomroy  KM Stowell  LM Gillespie 《New Zealand veterinary journal》2013,61(6):364-370

AIM: To isolate Neospora caninum from the brains of naturally infected cattle and use molecular techniques to characterise the isolates.

METHODS: Neospora caninum tachyzoites were isolated in Vero cell culture from the brains of a cow and two calves. The isolates were characterised using polymerase chain reaction (PCR) methods, DNA sequencing, an immunofluorescent anti-body test (IFAT), transmission electron microscopy (TEM), and immunohistochemistry (IHC). The brains of the three cattle were subjected to histopathological examination. A pathogenicity study was conducted in 120 BALB/c mice.

RESULTS: Neospora caninum tachyzoites were isolated from all three cases and first observed in vitro between 14 and 17 days post-inoculation. Parasites were sub-cultured and maintained in Vero cell culture for more than 6 months. PCR products were generated for all three isolates, using two different primers. Sequencing of the PCR products and a subsequent BLAST search identified the isolates as N. caninum. In addition, the isolates tested positive using IFAT and IHC, and ultrastructure revealed by TEM was characteristic of N. caninum. Histopathological examination revealed lesions characteristic of N. caninum in 1/3 brains. In the pathogenicity study using BALB/c mice, the mortality rate was 3–7%.

CONCLUSION: This was the first successful isolation of N. caninum in New Zealand confirmed using molecular characterisation tests.  相似文献   

Independent and combined effects of high temperature and drought stress around anthesis on wheat          下载免费PDF全文

A. Mahrookashani  S. Siebert  H. Hüging  F. Ewert 《Journal of Agronomy and Crop Science》2017,203(6):453-463

High temperature and drought stress are projected to reduce crop yields and threaten food security. While effects of heat and drought on crop growth and yield have been studied separately, little is known about the combined effect of these stressors. We studied detrimental effects of high temperature, drought stress and combined heat and drought stress around anthesis on yield and its components for three wheat cultivars originating from Germany and Iran. We found that effects of combined heat and drought on the studied physiological and yield traits were considerably stronger than those of the individual stress factors alone, but the magnitude of the effects varied for specific growth‐ and yield‐related traits. Single grain weight was reduced under drought stress by 13%–27% and under combined heat and drought stress by 43%–83% but not by heat stress alone. Heat stress significantly decreased grain number by 14%–28%, grain yield by 16%–25% and straw yield by 15%–25%. Cultivar responses were similar for heat but different for drought and combined heat and drought treatments. We conclude that heat stress as imposed in this study is less detrimental than the effects of those other studied stresses on growth and yield traits.  相似文献   

Parasites of sheep dogs in the Charleville district, Queensland     

KM CORNACK  PK O'ROURKE 《Australian veterinary journal》1991,68(4):149-149

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Measurement of creatine kinase MB in canine cardiac patients     

KM WYATT  R. LABUC  GL WYATT 《Australian veterinary journal》1998,76(12):826-826

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