Detection of hemorrhagic septicemia virus of salmonid fishes by use of an enzyme-linked immunosorbent assay containing high sodium chloride concentration and two noncompetitive monoclonal antibodies against early viral nucleoproteins.     

F A Sanz  J M Coll 《American journal of veterinary research》1992,53(6):897-903

Inclusion of high-ionic strength buffers helped us to develop a sandwich ELISA to detect hemorrhagic septicemia virus (HSV) in cell culture and infected trout tissue extracts. For maximal sensitivity of 0.1 to 0.2 ng/well/100 microliters or about 10 to 50 TCID50/well/100 microliters, trout extracts were diluted 1:1 and assayed for the earliest synthesized nucleoprotein N. Simultaneous binding of the N protein from HSV in the sample to the wells coated with monoclonal antibody (2D5 against the N protein) and to the peroxidase-labeled monoclonal antibody (2C9 against the N protein) proceeded during a 2-hour incubation at 20 to 22 C (room temperature). The response was linear between 6 to 60 ng/well of purified virus. Monoclonal antibodies used were noncompetitive with each other and reacted with F1, F2, 23.75, and 5 Spanish isolates of HSV, but not with infectious hematopoietic necrosis or infectious pancreatic necrosis viruses. Tissue specimens with low content of HSV virus may now be assayed directly without use of cell culture, rapidly, and with high precision, during the acute phase of the disease in salmonid fishes.  相似文献   

Effects of predator-induced visual and olfactory cues on 0+ perch (Perca fluviatilis L.) foraging behaviour     

V. N. Mikheev  J. Wanzenböck  A. F. Pasternak 《Ecology of Freshwater Fish》2006,15(2):111-117

Abstract –  Foraging juvenile fish with relatively high food demands are usually vulnerable to various aquatic and avian predators. To compromise between foraging and antipredator activity, they need exact and reliable information about current predation risk. Among direct predator-induced cues, visual and olfactory signals are considered to be most important. Food intake rates and prey-size selectivity of laboratory-reared, naive young-of-the-year (YOY) perch, Perca fluviatilis , were studied in experiments with Daphnia magna of two size classes: 2.8 and 1.3 mm as prey and northern pike, Esox lucius , as predator. Neither total intake rate nor prey-size selectivity was modified by predator kairomones alone (water from an aquarium with a pike was pumped into the test aquaria) under daylight conditions. Visual presentation of pike reduced total food intake by perch. This effect was significantly more pronounced (synergistic) when visual and olfactory cues were presented simultaneously to foraging perch. Moreover, the combination of cues caused a significant shift in prey-size selection, expressed as a reduced proportion of large prey in the diet. Our observations demonstrate that predator-induced olfactory cues alone are less important modifiers of the feeding behaviour of naive YOY perch than visual cues under daylight conditions. However, pike odour acts as a modulatory stimulus enhancing the effects of visual cues, which trigger an innate response in perch.  相似文献   

The use of a Giardia vaccine as an immunotherapeutic agent in dogs.          下载免费PDF全文

M E Olson  C J Hannigan  P F Gaviller    L A Fulton 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(11):865-868

Dogs (n = 13), which had failed to be cured of giardiosis following chemotherapeutic measures, were treated with a Giardia vaccine (2-3 injections). Clinical signs resolved between 16 and 42 days postvaccination and cessation of fecal cyst shedding was between 21 and 70 days. Vaccination is a potential method of treating giardiosis in dogs.  相似文献   

Site index estimation in Scots pine (Pinus sylvestris L.) stands in the High Ebro Basin (northern Spain) using soil attributes     

Bravo  F.; Montero  G. 《Forestry》2001,74(4):395-406

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Canine distal renal tubular acidosis and urolithiasis   总被引：1，自引：0，他引：1  

D J Polzin  C A Osborne  F W Bell 《Veterinary Clinics of North America: Small Animal Practice》1986,16(2):241-250

Distal RTA is characterized by decreased distal renal tubular hydrogen ion secretion, decreased ability to acidify urine, hypercalciuria, hyperphosphaturia, hypocitraturia, and metabolic acidosis. Because of the resulting alterations in urine composition and pH, patients with distal RTA are predisposed to urolithiasis and renal calcification. Diagnosis of distal RTA is important because it is a potentially reversible disorder that, left untreated, may cause nephrocalcinosis, recurrent urolith formation, moderate to severe metabolic acidosis, and renal failure.  相似文献   

Qntgegnung auf die im Abgeordnetenhaufe zu Berlin in der Sisung vom 3. Decbr. 1867 gegen das hannover’fche Forftmefen gefallenen Aeuserungen. Qin Schreiben des Forftdirectors Burdhardt an bie Mitglieder bes Landtags aus der Brobing Hannober. 14 Seiten in 4°. Hannober. Q. Rümpler 1868. Breis 18 fr     

F. Baur 《European Journal of Forest Research》1868,12(1):113-120

Ohne Zusammenfassung  相似文献   

ADDRESS OF THE PRESIDENT     

Lillie FR 《Science (New York, N.Y.)》1938,87(2263):421-423

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Some Ojibwa and Dakota practices     

Babbitt FE 《Science (New York, N.Y.)》1886,7(175):526-528

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ALCOHOLIC FERMENTATION BY FUSARIA JUICE OBTAINED WITH A WET CRUSHING MILL     

Wirth JC  Nord FF 《Science (New York, N.Y.)》1940,92(2375):15-16

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The effects of experimentally induced fever on the estimated blood flow to and oxygen utilization by the liver and the viscera drained by the portal vein in sheep     

D. N. Kisauzi  B. F. Leek 《Veterinary research communications》1991,15(2):95-105

Intrajugular injection of a purified E. coli lipopolysaccharide induced a biphasic fever in sheep after a latent period of 12 to 20 min. The changes in the blood flow from the liver and from the viscera drained by the portal vein were: (a) in the latent period, decreases in total hepatic blood flow (THF) due to decreased portal venous blood flow (PVF); (b) during the first febrile phase, increases in THF due to increased hepatic arterial blood flow and, (c) in the second febrile phase, increases in THF due to decreased PVF. Although there were large variations in the oxygen supply to the viscera drained by the portal vein and to the liver, there were relatively small or no changes in their oxygen consumption.  相似文献