酿酒山葡萄引种的试验研究     

王厚龙  李传键  法焕秀  王霞  郭振贵 《北方园艺》2006,(5):36-37

2001~2005年,引种8个山葡萄种内和种间杂交品种的试验结果表明:筛选出适宜当地气候和土壤条件、抗寒、产量高、可酿造干红葡萄酒左优红、加工果汁饮料的哈桑、用于调色和调酸的双优和双红,为当地主栽和推广品种。  相似文献   

Morphological and molecular characterization of <Emphasis Type="Italic">Diaporthe (</Emphasis>anamorph <Emphasis Type="Italic">Phomopsis)</Emphasis> complex and pathogenicity of <Emphasis Type="Italic">Diaporthe aspalathi</Emphasis> isolates causing stem canker in soybean     

Bruna B. Brumer  Valéria S. Lopes-Caitar  Aline S. Chicowski  Jessica D. Beloti  Fernanda M. Castanho  Danielle C. Gregório da Silva  Sandremir de Carvalho  Ivani O. N. Lopes  Rafael M. Soares  Claudine D. S. Seixas  Ricardo V. Abdelnoor  Francismar C. Marcelino-Guimarães 《European journal of plant pathology / European Foundation for Plant Pathology》2018,151(4):1009-1025

The complex of Diaporthe (asexual morph) species occurring on soybean constitutes an important pathogenic group associated with diseases such as pod and stem blight, seed decay and stem canker. Stem canker, caused by Diaporthe aspalathi, has been reported as the most aggressive form of canker and its occurrence has limited soybean crop productivity in the southern United States. The main form of pathogen control is the use of stem canker resistant soybean varieties. In this study, strains of Diaporthe and Phomopsis were isolated from stem and seeds of soybean in different locations in South America during the years 1989–2014. Genomic DNA from 26 isolates were analyzed by PCR-restriction fragment length polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) techniques, and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA. The molecular analysis of ITS sequences by alignment with those of ex-type strains deposited in GenBank and morphological characteristics allowed the identification of Phomopsis longicolla, D. phaseolorum var. sojae, D. caulivora and D. aspalathi. An analysis of the pathogenicity of 13 isolates of D. aspalathi inoculated in soybean genotypes carrying different resistance genes to stem canker (Rdm1, Rdm2, Rdm3, Rdm4, Rdm5 and Rdm?) enabled us to identify the occurrence of at least three races of D. aspalathi occurring in Brazil. Among the isolates identified as D. aspalathi, both molecular and phenotypic analyses showed clustering depending on the date of collection and pathogenicity, which revealed the existence of variability of the pathogen.  相似文献   

甘南高原独一味间作栽培藏木香对独一味生长的影响     

何淑玲  王改花  董利俊  齐军  汤文江  法小梅  王丽  尤艳娇 《广东农业科学》2012,39(9):20-21,34

采用田间随机区组法研究甘南高原独一味间作栽培藏木香对独一味出苗率、成活率、叶长、叶宽和株高的影响。结果表明,独一味间作栽培藏木香时,当年生独一味的出苗率、成活率比单作极显著提高;两年生独一味的叶长、叶宽和株高的生长变化也比单作显著加快。该研究攻克了独一味大田栽培中成活率低的技术难关,对独一味的人工栽培有一定的指导意义。  相似文献   

Environmental factors influencing the transmission of Haemonchus contortus     

Santos MC  Silva BF  Amarante AF 《Veterinary parasitology》2012,188(3-4):277-284

Infection with the gastrointestinal nematode Haemonchus contortus causes considerable losses in the sheep industry. In this study, we evaluated the effect that climate has on third-stage larvae (L3) of H. contortus in terms of their migration from sheep feces to Brachiaria decumbens grass, as well as their distribution among the forage plants. Fecal samples containing H. contortus L3 was deposited on the soil among the herbage at an initial height of 30 cm. Sample collection began 24h after contamination and was performed on alternate days over 13 days. The L3 were recovered and quantified in three strata (heights) of grass (0-10 cm, 10-20 cm and >20 cm) as well as in the remaining feces and a superficial layer of soil, collected from beneath the feces. In order to obtain results under different environmental conditions, fecal samples containing H. contortus L3 were deposited on pasture in January (summer), in April (autumn), and July (winter). In all of the periods, the L3 were able to migrate from the feces to the herbage. However, rains, accompanied by high relative humidity and high temperatures, apparently favored migration. The highest L3 recovery rate in the pasture was in the summer observation period, which had the highest number of days with measurable precipitation, high relative humidity (>68.2%), and the highest temperatures at the soil level (minimum and maximum means of 19°C and 42°C, respectively). Under those conditions, larvae began to reach the upper stratum of the grass (>20 cm) by 24h after the deposition of fecal matter, the number of larvae having reached that stratum peaking at seven days after deposition. In the autumn observation period, there was no rainfall in the first five days post-contamination. During that period, high numbers of larvae were found in the fecal samples demonstrating that feces can act as a reservoir of larvae in the absence of rain. Except for two days in the summer observation period, when most of the L3 were recovered from the tops of blades of grass, L3 where located predominantly at the base of the herbage. In conclusion, rainfall favors the migration of L3 from feces to herbage. In addition, larval migration up and along blades of grass can occur relatively rapidly when the temperature is high.  相似文献   

不同基因型烤烟叶片光合作用对光和CO2的响应研究     

李炜  符云鹏  翟欣  陈雪  黄化刚  喻奇伟  法鹏飞 《河南农业大学学报》2012,46(6)

为揭示不同基因型烤烟的光合特性,通过大田试验,利用LI-6400XT光合测定系统测定了毕节烟区4种主栽品种上部叶片的光、CO2的响应曲线,分别利用Farquhar模型和直角双曲线模型拟合光、CO2曲线,阐述了4个品种光响应和CO2响应特征及其主要参数.结果表明,4个品种响应曲线规律基本一致,响应特征参数差异明显.云烟97对高光照的适应能力相对最强、碳同化能力也表现较好;K326则表现出对弱光和低摩尔分数CO2的利用效率,对环境适应能力较强;毕纳1号饱和CO2速率明显高于其余品种,具有一定的高产潜力;红花大金元的优势体现在自身碳同化过程中的羧化效率.高海拔区域,选择种植云烟97能发挥其适应高光照的优势;中低海拔区域,K326和毕纳1号适应性较好,且能更好地发挥其生产潜力.  相似文献   

Detection and isolation of coronavirus from feces of three herds of feedlot cattle during outbreaks of winter dysentery-like disease     

Cho KO  Halbur PG  Bruna JD  Sorden SD  Yoon KJ  Janke BH  Chang KO  Saif LJ 《Journal of the American Veterinary Medical Association》2000,217(8):1191-1194

Clinical signs of a winter dysentery-like syndrome in 6- to 9-month-old cattle in 3 feedlots included acute onset of diarrhea with high morbidity and low mortality, respiratory tract problems that included dyspnea, coughing, and nasal discharge, and high rectal temperatures. Bovine coronavirus was detected by use of an ELISA and immune electron microscopy in fecal and nasal swab samples and by immunohistochemical analysis of intestinal sections collected from calves during necropsy. Bovine coronavirus should be considered in the differential diagnoses for diseases that cause acute onset of bloody diarrhea in feedlot cattle.  相似文献   

猪繁殖和呼吸综合征的研究进展     

FA Osorio  唐文渊 《国外畜牧学(猪与禽)》2003,23(2):55-56

从近二年来已经发表或即将发表的文献来看,猪繁殖和呼吸综合征的研究已经取得了一些极为显著的进展。  相似文献   

Pathological and microbiological characterization of mastitis in dairy cows     

Bianchi  Ronaldo M.  Schwertz  Claiton I.  de Cecco  Bianca S.  Panziera  Welden  De Lorenzo  Cíntia  Heck  Lilian C.  Snel  Gustavo G. M.  Lopes  Bruna C.  da Silva  Fernando S.  Pavarini  Saulo P.  Driemeier  David 《Tropical animal health and production》2019,51(7):2057-2066

Mastitis may be caused by a wide range of microorganisms able to induce distinct lesions in mammary tissues. This study aims to characterize the gross and microscopic features of mastitis in dairy cows and to correlate them with the pathogens involved. The udders of slaughtered dairy cows were inspected and milk samples from each mammary quarter or fragments of the parenchyma were sent for microbiological analysis, and tissue collected for histopathological evaluation. A total of 148 cows and 592 mammary quarters were collected. From these, 432 quarters (73%) had mastitis and in 160 (27%), no changes were observed. Mastitis was classified into seven patterns based on the histopathological findings, of which mixed, lymphoplasmacytic, and suppurative mastitides were the most prevalent with 35.9% (155/432), 27.1% (117/432), and 14.3% (62/432) of the cases, respectively. These patterns were associated with the same set of pathogens: Streptococcus spp., coagulase-negative Staphylococcus (CNS), Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, and Corynebacterium bovis. The pyogranulomatous pattern represented 7.2% (31/432) of the cases with distinct distribution based on the agent involved, mostly S. aureus and Nocardia sp. Abscedative mastitis accounted for 6.0% (26/432) of the cases; it was characterized by multiple abscesses in the parenchyma and was mainly caused by Trueperella pyogenes. Necrosuppurative mastitis represented 5.8% (25/432) of the cases which were characterized by severe parenchyma necrosis and were caused by bacteria such as CNS and Escherichia coli. The granulomatous pattern represented 3.7% (16/432) of the cases and was occasionally associated with Mycobacterium sp.

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Effect of high‐density lipoprotein on oocyte maturation and bovine embryo development in vitro     

Joao Alveiro Alvarado Rincn  Jorgea Pradie  Mariana Hrter Remio  Tiago Veiras Collares  Bruna Mion  Bernardo Garziera Gasperin  Monique Tomazele Rovani  Marcio Nunes Corrêa  Ligia Margareth Cantarelli Pegoraro  Augusto Schneider 《Reproduction in domestic animals》2019,54(3):445-455

High‐density lipoprotein (HDL) is the main lipoprotein in the follicular fluid, and it has anti‐inflammatory, antioxidant and cryoprotectant properties. The anti‐inflammatory potential and antioxidant potential are derived from its lipid composition, especially the apolipoprotein AI (ApoAI) and paraoxonase 1 (PON1). The aim of this study was to evaluate the effect of HDL during in vitro maturation (IVM) on oocyte maturation and early bovine embryo development. For this, cumulus–oocyte complexes (COCs) were obtained from bovine ovaries collected at a local slaughterhouse. COCs (n = 2,250) were allocated into three groups (n = 50 COCs/group) according to the addition of HDL protein (HDL‐P) during IVM for 22 hr: 0 (control), 50 and 150 mg/dl. After IVM, COCs were inseminated (in vitro fertilization) and cultivated for 7 days. Total cholesterol concentration, total protein, triglycerides and ApoAI concentrations on IVM medium increased proportionally to HDL‐P addition. However, PON1 activity was not detected in any treatment. The addition of HDL‐P did not affect nuclear maturation rate, endogenous reactive oxygen species and glutathione levels in COCs (p > 0.05). The highest HDL‐P concentration (150 mg/dl) decreased cleavage and blastocyst rate (p < 0.05). Moreover, the HDL‐P 150 mg/dl group had lower cellular count/blastocyst than the 50 mg/dl group (p < 0.05). However, the addition of HDL‐P did not affect relative gene expression of evaluated genes. In conclusion, the complex HDL/ApoAI obtained from human plasma, in the absence of PON1 activity during in vitro oocyte maturation, decreased initial embryo development.  相似文献   

Endometrial nitric oxide synthase activity in mares susceptible or resistant to persistent breeding‐induced endometritis and the effect of a specific iNOS inhibitor in vitro          下载免费PDF全文

FA Khan  TS Chenier  RA Foster  J Hewson  EL Scholtz 《Reproduction in domestic animals》2018,53(3):718-724

Emerging research suggests that the nitric oxide system may play a role in persistent breeding‐induced endometritis (PBIE) in the mare. Differences in uterine nitric oxide (NO) levels between mares susceptible or resistant to PBIE and a dose‐dependent inhibitory effect of NO on uterine contractility have been demonstrated. The objectives of this study were to investigate the difference in total nitric oxide synthase (NOS) activity of the endometrium between susceptible and resistant mares and the effect of a specific inducible nitric oxide synthase (iNOS) inhibitor on the endometrial NOS activity in vitro. Six susceptible and six resistant mares were selected based on preset criteria and the results of an intrauterine challenge with killed spermatozoa during oestrus. Endometrial biopsy samples were collected 24 hr post‐challenge and cultured at 37°C for 24 hr in L‐arginine supplemented minimum essential medium with or without a specific iNOS inhibitor (1,400 W dihydrochloride, 1 mM). The medium and the cultured endometrial tissue were collected after 24 hr of culture and assayed for NO and total protein, respectively. Total NO content of the medium, normalized to endometrial tissue wet weight or total protein, was used as a measure of endometrial NOS activity. Non‐parametric tests were applied for statistical analysis. Susceptible mares had significantly greater endometrial NOS activity than resistant mares. The iNOS inhibitor treatment significantly reduced NOS activity in endometrial samples derived from susceptible and resistant mares. These findings provide a basis for in vivo testing of specific iNOS inhibitors as preventative or therapeutic options for PBIE in mares.  相似文献