Tissue distribution of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) genome in experimentally infected juvenile European seabass (Dicentrarchus labrax)     

Lopez-Jimena B  Alonso Mdel C  Thompson KD  Adams A  Infante C  Castro D  Borrego JJ  Garcia-Rosado E 《Veterinary microbiology》2011,154(1-2):86-95

The distribution of viral genome in the tissues of juvenile European seabass (Dicentrarchus labrax) during the course of a Red Spotted Grouper Nervous Necrosis Virus (RGNNV) infection has not yet been described. The present study addresses this and indicates which target organs may be involved in viral replication. This information should enable more accurate detection of virus in asymptomatic carriers, and in turn help to control the spread of the disease. The aim of this study was to examine the pattern of expression of viral genomic segments RNA1 and RNA2, using two absolute real-time PCRs (RT-qPCR), over the course of a RGNNV infection after administering the virus by intramuscular injection. In situ hybridization was also used to locate the RNA2 viral segment in different organs throughout the infection. The experimental challenge provoked an acute form of viral nervous necrosis (VNN), with a resulting cumulative mortality of 37%. The RT-qPCRs designed allowed the detection of both genomic segments in all the organs tested (nervous and non-nervous tissues) at all sampling times examined. The highest viral RNA copy number was found in eyes, although viral replication appeared to begin in the brain. Viral replication was also recorded in pooled internal organs and in caudal fin. However, the increase in the viral RNA copy number in these organs did not result in an increased viral titre, which may indicate that a productive infection does not take place in non-nervous tissues, possibly due to a failure in a viral post-replication step.  相似文献   

Erratum to: A Simple and Accurate Method to Measure Total Gaseous Mercury Concentrations in Unsaturated Soils     

Christopher W. Moore  Mark S. Castro  Steven B. Brooks 《Water, air, and soil pollution》2011,218(1-4):11-11

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Heavy Metal Accumulation in Plant Species Indigenous to a Contaminated Portuguese Site: Prospects for Phytoremediation     

Moreira  Helena  Marques  Ana P. G. C.  Rangel  António O. S. S.  Castro  Paula M. L. 《Water, air, and soil pollution》2011,222(1-4):377-389

Acid rock drainage (ARD) often contains ferrous iron, sulphate and high concentrations of trace elements detrimental to the environment. Future costs will be enormous if the problem is not treated today. Simple and cost-effective methods for remediation of historical mine sites are therefore desired. In this study, three mine waters were mixed with alkaline ash leachates, and the fate of trace elements from both the mine waters (Cd, Cu, Pb, Zn, Ni and Co) and the ash leachates (Cr and Mo) was studied. Addition of ash water precipitates hydrous ferric oxides (HFO) and hydrous aluminium oxides (HAO) induced trace element sorption and coprecipitation. Composition of the formed HFO/HAO mix determines efficiency of the sorption and the relative order of sorption for different trace elements. Sorption occurred much earlier (often one pH unit or more) in a system with high iron concentrations compared to systems with lower iron concentrations. Removal of cadmium and zinc was low, below pH 8, if the amount of precipitates was low. Using ash for generation of alkaline water may be a problem with regard to chromium and molybdenum. This study shows that it is possible to avoid problems with molybdenum by keeping the final pH around 7, and chromium(VI) from the ash water will be reduced into chromium(III) and precipitated as the hydroxide in the presence of iron(II). Results imply that it is possible to also use fly ashes in alkaline leach beds in order to neutralize ARD followed by precipitation and sorption of trace elements.  相似文献   

Increasing consumption of ultra-processed foods and likely impact on human health: evidence from Brazil     

Monteiro CA  Levy RB  Claro RM  de Castro IR  Cannon G 《Public health nutrition》2011,14(1):5-13

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机电设备安装运行的异常现象及对策   总被引：1，自引：0，他引：1  

法书学 《湖南农机》2011,38(1):100-101

目前,由于国产设备的技术水平和加工水平,与国外的技术设备有较大的差距,关键设备大多引进国外技术设备,其整体及配件价格十分昂贵.为了提高企业经济效益,这就要求机电设备维护人员对设备的一些异常现象,做到分析原因提出解决对策,降低设备故障减少设备维修费用.  相似文献   

Influence of simulated deep frying on the antioxidant fraction of vegetable oils after enrichment with extracts from olive oil pomace     

Orozco-Solano MI  Priego-Capote F  Luque de Castro MD 《Journal of agricultural and food chemistry》2011,59(18):9806-9814

The stability of the antioxidant fraction in edible vegetable oils has been evaluated during a simulated deep frying process at 180 °C. Four edible oils (i.e., extra-virgin olive oil with a 400 μg/mL overall content in naturally existing phenols; high-oleic sunflower oil without natural content of these compounds but enriched either with hydrophilic antioxidants isolated from olive pomace or with an oxidation inhibitor, dimethylsiloxane; and sunflower oil without enrichment) were subjected to deep heating consisting of 20 cycles at 180 °C for 5 min each. An oil aliquot was sampled after each heating cycle to study the influence of heating on the antioxidant fraction composed of hydrophilic and lipophilic antioxidants such as phenols and tocopherols, respectively. The decomposition curves for each group of compounds caused by the influence of deep heating were studied to compare their resistance to oxidation. Thus, the suitability of olive pomace as raw material to obtain these compounds offers an excellent alternative to the use of olive-tree materials different from leaves. The enrichment of refined edible oils with natural antioxidants from olive pomace is a sustainable strategy to take benefits from this residue.  相似文献   

Delinted cottonseed in diets for finishing sheep     

Castro  W. J. R.  Zanine  A. M.  Ferreira  D. J.  Souza  A. L.  Pinho  R. M. A.  Parente  M. O. M.  Parente  H. N.  Santos  E. M. 《Tropical animal health and production》2020,52(5):2461-2468

Tropical Animal Health and Production - The use of agricultural by-products might provide an important alternative to improve animal agriculture and to reduce feeding costs. The effects of...  相似文献   

Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci     

Janira Prichula  Muriel Primon-Barros  Romeu C. Z. Luz  Ícaro M. S. Castro  Thiago G. S. Paim  Maurício Tavares  Rodrigo Ligabue-Braun  Pedro A. d&#x;Azevedo  Jeverson Frazzon  Ana P. G. Frazzon  Adriana Seixas  Michael S. Gilmore 《Marine drugs》2021,19(6)

New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host health. Here, we identified and characterized biosynthetic gene clusters encoding antimicrobial compounds in host-associated enterococci recovered from fecal samples of wild marine animals remote from human-affected ecosystems. Putative biosynthetic gene clusters in the genomes of 22 Enterococcus strains of marine origin were predicted using antiSMASH5 and Bagel4 bioinformatic software. At least one gene cluster encoding a putative bioactive compound precursor was identified in each genome. Collectively, 73 putative antimicrobial compounds were identified, including 61 bacteriocins (83.56%), 10 terpenes (13.70%), and 2 (2.74%) related to putative nonribosomal peptides (NRPs). Two of the species studied, Enterococcus avium and Enterococcus mundtti, are rare causes of human disease and were found to lack any known pathogenic determinants but yet possessed bacteriocin biosynthetic genes, suggesting possible additional utility as probiotics. Wild marine animal-associated enterococci from human-remote ecosystems provide a potentially rich source for new antimicrobial compounds of therapeutic and industrial value and potential probiotic application.  相似文献   

Inhibition of anthracnose symptoms in common bean by treatment of seeds with essential oils of Ocimum gratissimum and Syzygium aromaticum and eugenol     

Silva  Adriene Aparecida  Pereira  Fernanda Aparecida Castro  de Souza  Elaine Aparecida  de Oliveira  Denilson Ferreira  Nobre  Danúbia Aparecida Costa  Macedo  Willian Rodrigues  Silva  Geraldo Humberto 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):865-874

European Journal of Plant Pathology - Essential oils (EOs) of Ocimum gratissimum and Syzygium aromaticum and eugenol were applied to common bean seeds harvested from plants with anthracnose...  相似文献   

Effect of water hyacinth (Eichhornia crassipes) hay inclusion in the diets of sheep     

Gustavo Araújo de Vasconcelos  Robson Magno Liberal Véras  Janaina de Lima Silva  Daniel Barros Cardoso  Pierre de Castro Soares  Nadja Nara Gomes de Morais  Andresa Cristina Souza 《Tropical animal health and production》2016,48(3):539-544

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