玉米根部萜类合酶基因对干旱与激素处理的表达差异分析     

侯婧怡  傅竞也  赵颖  邱瑶  李聪聪  王强 《玉米科学》2017,25(5):63-67

选用两个具有抗性差异的玉米自交系B73与Mo17,分别对其根部进行干旱、脱落酸(ABA)、茉莉酸甲酯(MeJA)以及茉莉酸甲酯与乙烯利联合(MeJA/EP)处理,定量检测4个与防御相关的萜类合酶基因An2、ZmTPS6、ZmTPS8以及ZmTPS26的表达情况。结果表明,4个基因在B73与Mo17应对干旱和ABA处理时基因表达差异明显。在Mo17中,干旱胁迫以及ABA处理显著诱导4个基因的表达;在B73中,干旱处理导致An2、ZmTPS6和ZmTPS8基因表达下调,在ABA处理下时,只有ZmTPS6被显著上调;MeJA与MeJA/EP处理在B73与Mo17中都能上调4个基因的表达,但在Mo17中的诱导程度更剧烈。B73与Mo17的根部在响应胁迫时存在显著的萜类代谢差异,可能为其抗性差异原因之一。  相似文献   

吉林省玉米推荐施肥指标体系的建立   总被引：3，自引：0，他引：3  

冯国忠  焉莉  王寅  王少杰  李金昊  陈新平  崔振岭  樊小林  高强 《玉米科学》2017,25(6):142-147

通过总结2004~2014年间课题组在吉林省开展的田间试验及2005~2013年吉林省玉米"3414"田间肥效和校验试验资料,按土壤养分(播前耕层土壤硝态氮、速效磷、速效钾及10叶期0~90 cm土层土壤硝态氮)含量对土壤进行分级,进一步结合目标产量,初步建立以根层硝态氮调控为核心的总量控制、分期实时实地精确监控的玉米氮肥推荐施肥指标体系以及基于养分平衡和土壤测试的磷、钾肥恒量监控推荐施肥指标体系,为吉林省玉米合理科学施肥提供一定参考。  相似文献   

玉米新品种恩单105的选育及配套栽培技术     

朱祥芬  冯春福  王静  王黎明 《种子》2017,(3):106-109

恩单105是思施州农业科学院玉米小麦研究所以自育自交系S322作母本,自育自交系CJ 1作父本杂交育成的抗叶斑病的玉米新品种,该品种具有产量高、品质优、稳产、抗逆性强等优点,适宜湖北省春播区、中高海拔地区种植.本文对恩单105的高产配套栽培技术进行总结,为其在生产中的大面积示范推广提供科学依据.  相似文献   

玉米杂交制种测产方法及应用     

冯培煜  宋瑞连 《种子》2017,(3):132-134

测产是玉米杂交制种生产的重要环节,是基地技术员深入熟悉亲本特征特性的必要手段,其数据的精准与可靠,无论对改进生产技术,还是提高制种产量以及提高企业效益均具有现实意义.测产与预产、估产是有区别的;测产是利用仪器或工具来度量,即测绘、测量、测控得出的产量数据,其主要方法步骤有测产前准备、产量层级划分及测产取样点数(住)确定、田间测产取样、室内考种及汇总等.  相似文献   

日光温室蔬菜栽培废弃基质露地还田对土壤理化性质及辣椒生理特性和产量的影响     

张柏杨  张国斌  郁继华  冯致  杜淼鑫  罗石磊  叶洁  王翠丽 《华北农学报》2017,32(3)

为了研究基质还田对大田土壤和辣椒生长生产的影响,以日光温室连续栽培蔬菜6年的废弃基质还施露地种植辣椒,测定了不同施用量(0,45,90,135 m~3/hm~2)的土壤理化性质及辣椒生理特性和产量。结果表明:基质还田能够减少土壤pH变化,减小土壤容重、略微增加土壤孔隙度、保持土壤气水比,改善了土壤的理化性状,促进辣椒植株的生长且利于其干物质积累,其中株高、茎粗及干物质积累量均以T2最好。提升了辣椒叶片光合荧光的表现,T2的净光合速率、蒸腾速率、气孔导度、Fv/Fm、ΦPSⅡ均为最高,T1的q P最高,NPQ为T3最低。提高了辣椒果实的品质及产量,T2产量达67.04 t/hm~2,可溶性糖含量以T2最高,为2.21%,Vc含量T3最高,为1.97 mg/g,T1的可溶性蛋白含量最高、硝酸盐含量最低,分别为2.07,204.93 mg/kg。而与此同时,较大的还田施用量(135 m~3/hm~2)在辣椒生长的中前期造成了一定的毒害现象,在生育后期此现象消失,最终各项指标全面优于未还田处理。废弃基质露地还田这样的重复利用方式切实有效,(在种植同科作物的情况下)还田施用量宜选择90 m~3/hm~2。  相似文献   

亚硒酸钠浸种对春小麦种子萌发特性的研究     

冯魁  马艳明  田新年  贾永红  张金汕  迪里夏提·尔肯  刘俊  石书兵 《种子》2017,(9):39-43

为研究硒浸种对春小麦种子活力和幼苗生长特性的影响,分别设置0,0.5,1.0,2.5,3.5,5.0,10.0,50.0 mg/L共8个亚硒酸钠溶液浓度,通过发芽试验,比较研究了不同浓度的亚硒酸钠浸种对春小麦种子萌发和幼苗生长的影响.结果表明:随着亚硒酸钠溶液浓度升高,春小麦种子的发芽率、发芽势、发芽指数、活力指数、幼苗和根长以及干鲜重等指标均呈先升高后下降的趋势;1.0 mg/L的亚硒酸钠溶液对小麦种子萌发和幼苗生长有促进作用;随着亚硒酸钠浓度的升高(≥2.5 mg/L),其各项指标显著下降,并对根和苗的生长表现出抑制作用;因此在实际生产中,掌握合适的硒浸种浓度非常关键.  相似文献   

大麦品种驻大麦4号产量构成因素的通径分析     

冯辉  王树杰  郜战宁  薛正刚  杨永乾  潘正茂 《种子》2017,(7)

以驻大麦4号为材料,用2009-2015年度河南省区域试验和大麦品种展示数据汇总资料,进行驻大麦4号产量与产量构成因素相关性和通径分析.结果表明:驻大麦4号平均穗粒数51.39粒/穗,千粒重32.73 g,有效穗数568.79×104穗/hm2.株高、有效穗数和穗粒数变异系数较大.穗粒数和有效穗数与产量正相关,且穗粒数与产量相关系数达到极显著水平,千粒重与产量显著负相关;产量构成因素间相互制约,穗粒数与有效穗数负相关,与千粒重极显著负相关,有效穗数与千粒重负相关.产量与产量构因素间回归方程决定系数达到极显著水平,即驻大麦4号产量形成的89.8％以上是由产量构成因素贡献的.通径分析表明,穗粒数对驻大麦4号产量贡献最大,其次是有效穗数,千粒重相对较小;间接通径系数均为负值,说明驻大麦4号产量构成因素间制约作用较强,在适宜基本苗条件下,通过合理肥水运筹,稳定穗数,增加穗粒数,提高产量.  相似文献   

Correlation between Mnk2 protein expression and prognosis of patients with esophageal squamous cell carcinoma     

ZENG Bo  FU Min-yi  FENG Yan-fen  HAN Xiang-qian  ZOU Da-wei  LUO Hong-he  LEI Yi-yan 《园艺学报》2017,33(10):1864-1868

AIM: To investigate the protein expression of mitogen-activated protein kinase-interacting kinase-2 (Mnk2) and its prognostic effect in the patients with resected esophageal squamous cell carcinoma (ESCC). METHODS: A total of 86 informative patients with surgically resected ESCC and 54 normal esophageal tissues were enrolled. Western blot and immunohistochemistry (IHC) were utilized to assess the protein expression of Mnk2, and its correlation with prognosis was statistically analyzed by the methods of Kaplan-Meier curve and Cox proportional hazard mode. RESULTS: The protein expression of Mnk2 was elevated in most of tumor tissues compared with the adjacent tissues. Clinicopathologic analysis showed that Mnk2 expression was significantly correlated with the TNM stage (P<0.05). Both disease-free survival (DFS) and overall survival (OS) of Mnk2 over-expression patients were shorter than those in Mnk2 negative expression group. Multivariate analysis confirmed that Mnk2 expression, as an independent and significant factor for both DFS and OS, predicted a poor prognosis of the patients with resected ESCC (P<0.05). CONCLUSION: The expression of Mnk2 was significantly related to the TNM stages, and might be a novel predictor for prognosis in ESCC.  相似文献   

芸薹根肿菌单孢分离技术体系构建及沈阳地区根肿菌的鉴定     

刘一凡  王赛  常虹  高诗琪  赵颖  冀瑞琴  冯辉 《园艺学报》2017,44(12):2383-2390

芸薹根肿菌严重危害大白菜和其他十字花科作物。由于根肿菌通常以多个生理小种混生状态进行侵染,导致抗病品种的抗性不能持久。以沈阳地区根肿菌为材料,进行了单孢分离技术体系构建,结果表明采取冷冻盒接菌法对两日龄大白菜幼苗进行单孢接种,可将侵染率提高到47.86%;采用基质过渡培养可将大白菜植株成活率提高到67%。利用上述根肿菌单孢分离技术体系对沈阳地区根肿菌进行单孢分离和Williams生理小种鉴定,结果表明沈阳地区根肿菌存在4号、8号、9号、11号生理小种,其中4号生理小种为优势小种。  相似文献   

Celecoxib inhibits viability,induces apoptosis and inhibits autophagy in acute myeloid leukemia cell lines HL-60 and HL-60A     

LU Ying  LIU Xiang-fu  LIU Ling-ling  LIN Zhe-sheng  CHEN Yu-chan  FENG Bao-ying  ZHANG Xiang-zhong 《园艺学报》2017,33(1):18-25

AIM: To investigate the effects of celecoxib on viability, apoptosis and autophagy in acute myeloid leukemia (AML) cell lines HL-60 and HL-60A. METHODS: The HL-60 cells and HL-60A cells were cultured with various concentrations (0, 20, 40, 60, 80 and 100 μmol/L) of celecoxib. The inhibitory effect of celecoxib on the cell viability was evaluated by MTT assay. Apoptosis was analyzed by Annexin-V/PI staining. Apoptosis-related and autophagy-related proteins were determined by Western blot. RESULTS: IC₅₀ of celecoxib were 49.4 μmol/L, 32.0 μmol/L and 25.1 μmol/L for HL-60 cells treated with celecoxib for 24 h, 48 h and 72 h, respectively. For HL-60A cells, the corresponding IC₅₀ were 69.1 μmol/L, 42.5 μmol/L and 29.6 μmol/L, respectively. The results of flow cytometry analysis showed the proportions of Annexin-Ⅴ⁺ PI^-, Annexin-Ⅴ⁺ PI⁺ and Annexin-Ⅴ^-PI⁺ cells were increased in the HL-60 cells, and those of Annexin-Ⅴ⁺PI^- and Annexin-Ⅴ⁺ PI⁺ cells were increased in the HL-60A cells treated with celecoxib for 24 h. After treated with celecoxib, the induction of apoptosis was observed, the apoptosis-related proteins cleaved caspase-3 and cleaved PARP were upregulated, the autophagy-related proteins LC3 II and P62 were both increased, and mTOR, p-mTOR, 4-EBP and p-4-EBP were not changed, indicating that celecoxib inhibited autophagy in the AML cells without the mTOR pathway involvement. CONCLUSION: Celecoxib inhibits the viability of HL-60 cells and HL-60A cells in a time-and dose-dependent manner by its effects of inducing apoptosis and necrosis. Celecoxib inhibits mTOR-independent autophagy in AML cells, indicating a possible way of using celecoxib for enhancing the antitumor activity of therapeutic agents to induce cytoprotective autophagy in the AML cells.  相似文献