Effect of three feeding management systems on some reproductive parameters of Scorpion mud turtles (Kinosternon scorpioides) in Brazil     

Jamile da Costa Araújo  Priscila Vieira e Rosa  Maria das Dores Correia Palha  Paulo Borges Rodrigues  Rilke Tadeu Fonseca de Freitas  Alanna do Socorro Lima da Silva 《Tropical animal health and production》2013,45(3):729-735

Chelonians have been exploited since ancient times for their meat, eggs, fat, and offal, among other things. Among these animals, there is Scorpion mud turtle (Kinosternon scorpioides) which is an omnivorous turtle with semiaquatic habits and is widely consumed in Brazil. The aim of this study was to evaluate the influence of different feeding management systems on the reproductive performance of captive-bred Scorpion mud turtles, including egg quality and chemical composition. Study animals included 15 males and 48 females, divided into three groups. Animals were fed with either a commercial diet or a commercial diet supplemented with one of two different mixes (wet diet). All animals were fed at 1 % BW/day divided on Mondays, Wednesdays, and Fridays. Treatments were as follows: TR?=?100 % fish food (22 % CP); TRM1?=?70 % fish food?+?30 % mix 1 (bovine offal and fish); TRM2?=?70 % fish food?+?30 % mix 2 (bovine offal, fish, and shrimp). Samples were collected during 7 months, and eggs were identified, weighed, measured, and frozen for later analysis. Statistical analyses, including ANOVA, were performed using the program SAEG 9.0. Measurements taken from the nests were compared using the Tukey’s test (P?<?0.05). The different diets were associated with differential egg-laying performance and eggshell thickness. Animals fed with wet diets containing protein of animal origin displayed the best performance. Furthermore, nests with fewer eggs contained eggs of better external quality (e.g., greater length, width, and eggshell thickness). Finally, animals fed with only commercial feed produced eggs with lower saturated fatty acid content.  相似文献   

Serum amyloid A concentration in healthy periparturient mares and mares with ascending placentitis     

M. A. Coutinho da Silva  I. F. Canisso  M. L. MacPherson  A. E. M. Johnson  T. J. Divers 《Equine veterinary journal》2013,45(5):619-624

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Evaluation of equations to predict body composition in Nellore bulls     

L.F. Costa e Silva  S.C. Valadares FilhoE. Detmann  M.I. MarcondesP.P. Rotta  L.F. PradosD. Zanetti 《Livestock Science》2013,151(1):46-57

The equations developed by Hankins and Howe (1946, HH), Marcondes et al. (2010, M10), Marcondes et al. (in press, M11) and Valadares Filho et al. (2006, V6) were evaluated to predict the body composition from the 9–10–11th rib cut in Nellore bulls. The evaluated equations estimated the physical and the carcass chemical composition, the empty body chemical composition and the noncarcass chemical composition. Thirty-seven Nellore bulls (14±1 months old initially) with shrunk body weight of 259±24.9 kg were used in this experiment. The bulls were randomly divided into three groups: five bulls to the reference group, four bulls were fed at maintenance level and twenty-eight bulls were fed ad libitum. The bulls fed ad libitum were separated into four groups, one of which was slaughtered every 42 days. The diet was composed of corn silage and concentrate (55:45). After slaughter, the 9–10–11th rib cut was dissected into muscle, fat and bone fractions. The remaining carcass was similarly dissected. The others parameters that were evaluated as partial predictors included the empty body weight, the dressing percentage, the visceral fat percentage, the organ and viscera percentage and the composition of the noncarcass components. The values estimated with prediction equations were compared to the observed values. The equations obtained by M11 predicted correctly the carcass physical composition. However, the muscle and fat tissues were under- and overestimated, respectively, by HH. Some constituents of the noncarcass components can be predicted from equations developed by M10. The equations obtained by M10 predicted correctly the carcass and empty body chemical composition. The carcass water was underestimated by HH. The equations by V6 did not predict the carcass or empty body chemical composition. The carcass physical and chemical composition and empty body chemical composition can be predicted from the composition of 9–10–11th rib cut by equations obtained by Marcondes et al., 2010 and MarcondesPlease complete and update the reference given here (preferably with a DOI if the publication data are not known): Marcondes et al. (in press). For references to articles that are to be included in the same (special) issue, please add the words ‘this issue’ wherever this occurs in the list and, if appropriate, in the text. et al., while the composition of these components cannot be predicted by Hankins and Howe (1946) and Valadares Filho et al. (2006) in Nellore bulls.  相似文献   

Genomic Description of Antibiotic Resistance in Escherichia coli and Enterococci Isolates from Healthy Lusitano Horses     

Inês Moura  Carmen Torres  Nuno Silva  Sergio Somalo  Gilberto Igrejas  Patrícia Poeta 《Journal of Equine Veterinary Science》2013

Antibiotic resistance is a global problem, and it is known that commensal bacteria can act as reservoir of antibiotic resistance genes of clinical importance. The aim of the present study was to determine the antibiotic resistance phenotype and mechanisms implicated in resistance of Escherichia coli and Enterococcus spp. isolates collected from fecal samples of 90 Lusitano horses from Portugal. Sixteen of the 71 E. coli isolates (22.5%) recovered showed resistance to at least one of the antibiotics tested. The number of E. coli isolates resistant to streptomycin, tetracycline, chloramphenicol, ampicillin, trimethoprim-sulfamethoxazole, and gentamicin was 9, 7, 6, 3, 2, and 1, respectively. The bla_TEM-1 and bla_OXA-1 genes were detected in ampicillin-resistant isolates and the sul2 and dfrA1 genes in trimethoprim-sulfamethoxazole-resistant, while the aac(3)-I, floR and tet(A) were found in the gentamicin, chloramphenicol and tetracycline-resistant isolates, respectively. Twenty-two of the 71 (31%) recovered enterococci showed antibiotic resistance for at least one of the tested antibiotics, and resistant isolates were identified as Enterococcus faecium (n = 14), E. faecalis (n = 3), E. hirae (n = 2), and Enterococcus spp. (n = 3). The erm(B) and erm(C) genes were identified in erythromycin-resistant enterococci and the tet(M) and/or tet(L) genes in tetracycline-resistant isolates. The slight prevalence of antibiotic resistance among commensal bacteria of healthy Lusitano horses can improve the treatment of upcoming infections in these horses because these microorganisms can be considered as antimicrobial indicator bacteria.  相似文献   

The Correlation between Age,Body Weight and Testicular Parameters in Murrah Buffalo Bulls Raised in Brazil     

Patrícia Aparecida Cardoso da LUZ  Paulo Ramos da Silva SANTOS  Cristiana ANDRIGHETTO  André Mendes JORGE  Ant?nio Chaves de ASSIS NETO 《The Journal of reproduction and development》2013,59(1):14-17

Buffalo are an economically important source for meat and milk production, especially in Brazil. However, important aspects of their biology remain unknown thus far. Herein, we describe the reproductive characteristics of male Murrah buffalo (Bubalus bubalis) raised under extensive management conditions by applying biometrics associated with testicular weight. We analyzed seven males, divided into two groups: G1, which consisted of four 18-month-old animals, and G2, which consisted of three 24-month-old animals. Testicular development occurs slowly in Murrah buffalo, suggesting a delay of sexual maturity. The biometric testicular parameters analyzed were scrotal circumference, testicular weight, testicular length, testicular width, testicular thickness and testicular circumference. Our data indicate strong correlations between SC, age and body weight, and additional significant relationships were identified between body weight, age and other testicular parameters. Thus, these parameters are suitable indicators when selecting bulls for breeding purposes.  相似文献   

Chickens treated with a nitric oxide inhibitor became more resistant to Plasmodium gallinaceum infection due to reduced anemia,thrombocytopenia and inflammation     

Barbarella Matos de Macchi  Farlen José Bebber Miranda  Fernanda Silva de Souza  Eulógio Carlos Queiroz de Carvalho  Ant?nio Peixoto Albernaz  José Luiz Martins do Nascimento  Renato Augusto DaMatta 《Veterinary research》2013,44(1):8

Malaria is a serious infectious disease caused by parasites of the Plasmodium genus that affect different vertebrate hosts. Severe malaria leads to host death and involves different pathophysiological phenomena such as anemia, thrombocytopenia and inflammation. Nitric oxide (NO) is an important effector molecule in this disease, but little is known about its role in avian malaria models. Plasmodium gallinaceum- infected chickens were treated with aminoguanidine (AG), an inhibitor of inducible nitric oxide synthase, to observe the role of NO in the pathogenesis of this avian model. AG increased the survival of chickens, but also induced higher parasitemia. Treated chickens demonstrated reduced anemia and thrombocytopenia. Moreover, erythrocytes at different stages of maturation, heterophils, monocytes and thrombocytes were infected by Plasmodium gallinaceum and animals presented a generalized leucopenia. Activated leukocytes and thrombocytes with elongated double nuclei were observed in chickens with higher parasitemia; however, eosinophils were not involved in the infection. AG reduced levels of hemozoin in the spleen and liver, indicating lower inflammation. Taken together, the results suggest that AG reduced anemia, thrombocytopenia and inflammation, explaining the greater survival rate of the treated chickens.  相似文献   

Comparison of Different Glycerol and Egg Yolk Concentrations Added to Tris‐based Extender for the Collared Peccaries (Tayassu tajacu) Semen Freezing     

HM Alves  IRS Oliveira  TS Castelo  GL Lima  ALP Souza  MAP Moreira  VV de Paula  AR Silva 《Reproduction in domestic animals》2013,48(3):506-511

This study aimed to evaluate various concentrations of egg yolk (5, 10, or 20%) in combination with different concentrations of glycerol (3% or 6%) added to a Tris‐based extender on the post‐thaw characteristics of sperm obtained from Tayassu tajacu. For this purpose, semen from 10 sexually male mature collared peccaries was collected by electroejaculation and evaluated for sperm motility, vigour, viability, morphology and functional membrane integrity. The ejaculates were initially extended in Tris‐fructose plus egg yolk (5%, 10% or 20%). After cooling, the semen was added to Tris‐egg yolk plus glycerol (6% or 12%), resulting in a final concentration of 3% or 6% glycerol of the extender. Straws were frozen using liquid nitrogen and thawed in a water bath at 37°C for 30 s. The frozen–thawed semen was evaluated as reported for fresh semen. After thawing, a significant decrease was verified for sperm motility and vigour, for all the samples in comparison with fresh semen. However, no differences were evidenced among treatments for any sperm characteristics evaluated (p > 0.05), except for the combination between 10% egg yolk and 6% glycerol, which provided the worst preservation of functional membrane integrity (p < 0.05). The interactions between higher concentrations of egg yolk (20%) and glycerol (6%) and also between lower concentrations of the same substances (5% egg yolk and 3% glycerol) added to the Tris‐based extender negatively affected the preservation of the normal sperm morphology after thawing (p < 0.05). In conclusion, the use of Tris‐based extender added to 10% or 20% egg yolk plus 3% glycerol is recommended for effective sperm cryopreservation in collared peccaries.  相似文献   

Apparent electrical conductivity in dry versus wet soil conditions in a shallow soil     

João M. Serrano  Shakib Shahidian  José R. Marques da Silva 《Precision Agriculture》2013,14(1):99-114

The general objective of this study was to evaluate the stability of patterns of apparent soil electrical conductivity (EC_a) in dry versus wet soil conditions in a shallow soil typically used for pastures in Mediterranean conditions of the southern region of Portugal. A 6 ha experimental field of permanent bio-diverse pasture was divided into 76 squares of 28 × 28 m. The soil electrical conductivity was measured using a Dualem 1S sensor under dry conditions (June 2007) and under wet conditions during the rainy season (March 2010). Soil samples, geo-referenced with GPS, were collected in a depth range of 0–0.30 m. The soil was characterized in terms of bedrock depth, moisture content, texture, pH, organic matter content, and macronutrients (nitrogen, phosphorus, and potassium). Pasture samples, also geo-referenced with GPS, were collected to measure the pasture dry matter yield. The statistical analysis of apparent electrical conductivity between dry and wet soil conditions resulted in a linear significant correlation coefficient (R = 0.88). The results also showed a significant correlation between apparent electrical conductivity and the relative field elevation (R = ?0.64 and R = ?0.66), the pasture dry matter yield (R = 0.42 and R = 0.48), the bedrock depth (R = 0.40 and R = 0.27), the pH (R = 0.50 and R = 0.49), the silt (R = 0.27 and R = 0.38) and soil moisture content (R = 0.48 and R = 0.45), in dry and wet conditions, respectively. A multi-variate regression was carried out using the following soil parameters that showed significant correlation with EC_a and that did not present multi-collinearity: pH, bedrock depth, silt and moisture content. The results showed, in dry and wet conditions, that the analysis was significant (R = 0.75 and R = 0.84, respectively). Overall, these results indicate the temporal stability of EC_a patterns under different soil moisture contents, which is relevant with respect to the time when a field should be surveyed and is important for using the electrical conductivity sensor, as a decision support tool for management zones in precision agriculture.  相似文献   

Isolation and differential expression analysis of self-compatibility-related genes from mature pistils of ‘Shatangju’ mandarin (Citrus reticulata Blanco)     

H. X. Miao  Y. H. Qin  Jaime A. Teixeira Da Silva  Z. X. Ye 《The Journal of Horticultural Science and Biotechnology》2013,88(6):575-582

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Evaluating ecological‐niche factor analysis as a modelling tool for environmental weed management in island systems     

H Costa  V Medeiros  E B Azevedo  L Silva 《Weed Research》2013,53(3):221-230

Management actions are essential for mitigating the potentially harmful changes in biodiversity, ecosystem function and crop/forest productivity caused by invasive species. Species distribution models, if reliable, could be used to design effective management strategies. Although several modelling methods well suited for studying invasive species have been developed for presence‐only data, often the size of available data sets for modelling is small and results are not validated with test samples. Moreover, the impact of such methods in practical applications has been overlooked. Here, we evaluated the reliability of the modelling approach based on ecological‐niche factor analysis (ENFA) implemented in Biomapper software when applied to environmental weed data in the Azores. Presence‐only data sets of two top invasive woody species (Pittosporum undulatum and Acacia melanoxylon) were used. The continuous Boyce curve was used for validation, calculated either in Biomapper (cross‐validation) or based on test samples. The species' most habitable areas that should be regarded as management targets were thus estimated from modelling and validation. By imposing size restrictions on the presence‐only data sets used in modelling and validation, other habitable areas were defined and compared. The ENFA proved to be a suitable method for modelling environmental weed distributions, regardless of the presence‐only dataset size. Moreover, the cross‐validation of Biomapper was reliable, although its results should be interpreted with caution as they could potentially lead to statistically different management target areas.  相似文献