Linking spatio-temporal dynamics of earthworm populations to nutrient cycling in temperate agricultural and forest ecosystems: The 7th international symposium on earthworm ecology · Cardiff · Wales · 2002     

Joann K. Whalen  Carlos Costa 《Pedobiologia》2003,47(5-6):801-806

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Haemonchus contortus resistance to ivermectin and netobimin in Brazilian sheep.   总被引：1，自引：0，他引：1  

L S Vieira  M E Berne  A C Cavalcante  C A Costa 《Veterinary parasitology》1992,45(1-2):111-116

Suffolk, Texel, Hampshire Down and Ile de France sheep from the municipalities of Porto Amazonas, Piraquara and Araucaria in the State of Paraná, and Bagé in the State of Rio Grande do Sul were brought to Sobral, State of Ceará, to be used in a cross-breeding project. On arrival they had clinical signs of nematode parasitosis, and one Suffolk female died. The animals were treated orally with ivermectin (0.2 mg kg-1) and fifteen days later with netobimin (20.0 mg kg-1). Neither drug reduced the egg counts (measured in eggs per gram, EPG) significantly, and this suggested that the nematodes in the sheep were resistant to the anthelmintics used. Haemonchus contortus was the species involved. The egg counts were reduced after oral treatment with trichlorfon (100.0 mg kg-1). Haemonchus contortus larvae obtained from these animals before trichlorfon treatment and passaged through two nematode-free sheep were used in a further experiment. Twenty 6- to 9-month-old nematode-free lambs were infected with the H. contortus larvae (10,000 per animal) and after the infection was confirmed, were randomly divided into four groups of five animals. Group I was orally treated with ivermectin at 0.2 mg kg-1, Group II with oral netobimin at 20.0 mg kg-1, Group III with oral trichlorfon at 100.0 mg kg-1 and Group IV was a non-treated control. Egg counts and faecal cultures were taken before dosing on the day of treatment and seven days later when all animals were necropsied and the nematodes were collected from the abomasa and counted.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Occurrence of caprine arthritis-encephalitis in Mozambique     

C. Lopes Pereira  C. Baule  R. Costa  A. Langa 《Tropical animal health and production》1989,21(4):237-238

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Flow cytometric assay in peripheral blood of dogs--reference values for leukocytes from Brazilian beagles     

Fujiwara RT  Bueno LL  Vale AM  Franca-Silva JC  da Costa RT  Quetz Jda S  Machado-Coelho GL  Reis AB  Martins Filho OA  Genaro O  Nascimento E  Mayrink W 《Polish journal of veterinary sciences》2005,8(1):17-22

Use of domestic reference values in the flow cytometry analysis is known to improve its accuracy by integrating local variations as gender, race and age. Up to date application of flow cytometry in veterinary medicine has been limited to describe the percentual values just for peripheral lymphocytes subsets of blood. We now report establishment of reference values for a wide range of proportional and absolute numbers of peripheral blood leukocytes, including T cells subsets, B cells, monocytes and eosinophils, applicable to the healthy population of Beagles in Brazil and other regions with similar demographic characteristics. Normal reference values were also established to estimate the gender-related differences. This information will provide clinical aid in the evaluation of immunologic status as well as standard values for experimental animals of dogs from Brazil and other similar regions.  相似文献   

Characterization of antibiotic resistance genes and virulence factors in faecal enterococci of wild animals in Portugal     

Poeta P  Costa D  Sáenz Y  Klibi N  Ruiz-Larrea F  Rodrigues J  Torres C 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2005,52(9):396-402

Antibiotic susceptibility was tested in 140 non-selected enterococci (73 Enterococcus faecalis, 45 E. faecium and 22 of other species) recovered from faecal samples of 77 wild animals in Portugal. Susceptibility testing for 11 antibiotics (vancomycin, teicoplanin, ampicillin, streptomycin, gentamicin, kanamycin, chloramphenicol, tetracycline, erythromycin, quinupristin-dalfopristin and ciprofloxacin) was determined by disk diffusion and agar dilution methods. Forty-four isolates (31.4%) showed susceptibility to all the antibiotics tested (5.5% of E. faecalis; 62.2% of E. faecium; and 78.6% of E. hirae). Neither ampicillin-resistance nor acquired-vancomycin-resistance was detected and 1.4% of the isolates showed high-level-resistance for gentamicin or streptomycin. Tetracycline and erythromycin resistances were shown in 28.6% and 20.1% of the isolates, respectively. Antibiotic resistance genes were studied by polymerase chain reaction (PCR) and sequencing and tet(M) + tet(L), erm(B) or aac(6')-aph(2') genes were detected in most of tetracycline-, erythromycin- or gentamicin-resistant enterococci respectively. Genes encoding virulence factors were studied by PCR and a wide variety of virulence genes were detected in most of E. faecalis isolates but were rarely found in E. faecium and not detected in the other species. The prevalence of genes encoding virulence factors in E. faecalis was as follows: cpd (98.6%), gelE (75.3%), agg (30.1%), fsr (17.8%), ace (9.6%) and esp (4.1%). Low percentages of antibiotic resistance was found in the faecal enterococci of wild animals but a wide variety of virulence genes were detected among E. faecalis isolates although were rare in the other species.  相似文献   

Luteoforol,a flavan 4-ol,is induced in pome fruits by prohexadione-calciumand shows phytoalexin-like properties against <Emphasis Type="Italic">Erwinia amylovora</Emphasis>and other plant pathogens     

Francesco?SpinelliEmail author  John-Bryan?Speakman  Wilhelm?Rademacher  Heidi?Halbwirth  Karl?Stich  Guglielmo?Costa 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(2):133-142

Treatments with prohexadione-calcium led to lowered incidence of fire blight, scab and other diseases in pome fruit trees and other crop plants. In addition to acting as a growth regulator, prohexadione-calcium interferes with flavonoid metabolism and induces the accumulation of the 3-deoxycatechin luteoliflavan in shoots of pome fruit trees. Luteoliflavan does not possess any remarkable antimicrobial activity. Therefore luteoforol, its unstable and highly reactive precursor, has been tested in vitro for its bactericidal and fungicidal activities. Luteoforol was found to be highly active against different strains of Erwinia amylovora, the causal agent of fire blight, and all other bacterial and fungal organisms tested. Phytotoxic effects were also observed in pear plantlets. The results obtained indicate that prohexadione-calcium induces luteoforol as an active principle with non-specific biocidal properties. It is proposed that luteoforol is released upon pathogen attack from its cellular compartment and inhibits further disease development by destroying pathogen cells as well as by inducing a hypersensitive-like reaction in the host plant tissue. This mechanism would be closely analogous to the one known for structurally related phytoalexins in sorghum.  相似文献   

Daily rhythm of lactate dehydrogenase in rat (Rattus norvegicus) carrying a Per1-luciferase transgene: assessment on serum and liver     

Costa A  Castañón-Cervantes O  Menaker M  Piccione G  Caola G 《Veterinary research communications》2005,29(Z2):183-186

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Clinical disorders observed in anteaters (Myrmecophagidae,Edentata) in captivity     

L. S. M. Diniz  E. O. Costa  P. M. A. Oliveira 《Veterinary research communications》1995,19(5):409-415

The major health problems found in 103 captive lesser anteaters (Tamandua tetradactyla) and giant anteaters (Myrmecophaga tridactyla), family Myrmecophagidae, are presented and correlated with management. The most common of 200 recorded clinical disorders involved the digestive system (26%), nutritional deficiency (20%), injury (15.5%), respiratory system (10%), skin (7%) and circulatory system (4.5%), but 13% of the cases were inconclusive. Parasites were identified in 48.5% of faecal samples, mainly the eggs of nematodes (40%), of which the commonest wereTrichuris spp (28%) andStrongyloides spp (11%); protozoa (16%), of which the commonest wereEimeria spp (10%),Entamoeba spp (5%) andGiardia spp (1%); and cestodes (8%) and acanthocephalids (1%). Bacteria cultured from the various materials includedSalmonella enteritidis, S. cholerasuis, Escherichia coli, Enterobacter aerogenes, Streptococcus spp andStaphylococcus spp. The ectoparasites found wereAmblyomma spp andOtodectis spp (Arthropoda, Acaridae).  相似文献   

Experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings     

de Castro MB  Machado RZ  de Aquino LP  Alessi AC  Costa MT 《Veterinary parasitology》2004,119(1):73-86

Clinical signs, humoral and cellular immune responses, and microscopic and gross tissue alterations resulting from acute experimental Ehrlichia canis infection in dogs were studied. Four dogs were inoculated with E. canis and four were used as uninfected controls. After a 10-14-day incubation period, infected dogs developed pyrexia up to 41 degrees C for 6-8 days. Antibody titers to E. canis antigen were demonstrable in all inoculated dogs at 30 days post-infection. Necropsy of infected animals revealed pale mucous membranes, generalized lymphadenopathy, splenomegaly, edema and ascites. Microcopically, the main lesions were: lymphoreticular hyperplasia in cortical areas of lymph nodes and spleenic white pulp, periportal accumulation of mononuclear cells and centrolobular fatty degeneration of the liver. Kidneys presented with glomerulonephritis characterized by interstitial mononuclear infiltration. Immunophenotyping of lymphocytes from lymph nodes and spleen sections displayed alterations in IgG, IgM, CD3+ and CD8+ cells population in infected dogs.  相似文献   

Cervical syringohydromyelia secondary to a brainstem tumor in a dog     

da Costa RC  Parent JM  Poma R  Duque MC 《Journal of the American Veterinary Medical Association》2004,225(7):1061-4, 1048

An 11-year-old male Pekingese was evaluated because of a history of head tilt. Neurologic examination revealed a right-sided head tilt, ataxia, scoliosis, and proprioceptive deficits. Diagnostic testing included magnetic resonance imaging (MRI) of the head and neck. After IV administration of gadopentetate dimeglumine, an extra-axial, highly contrast-enhanced mass in the brainstem, cerebellar herniation, and syringohydromyelia were detected via MRI. The dog was treated with corticosteroids and radiation therapy of the mass for 4 weeks (total dose, 42.5 Gy). Magnetic resonance imaging was repeated 9 weeks and 6 months after radiation therapy; compared with the initial findings, a reduction in the size of the brainstem mass was observed in both MRI scans. The third MRI scan also revealed a normal cerebellar shape, no evidence of herniation, and resolution of syringohydromyelia in the dog at that time. It is recommended that whenever syringohydromyelia is observed via MRI, a primary cause (cranial or caudal to the affected region) should be sought.  相似文献