Prevalence of Haemophilus parasuis serovars isolated in Spain from 1993 to 1997   总被引：10，自引：0，他引：10  

Rúbies X  Kielstein P  Costa L  Riera P  Artigas C  Espuña E 《Veterinary microbiology》1999,66(3):245-248

From 1993 to 1997, 327 strains of Haemophilus parasuis were isolated from spanish swine in our Diagnostic Laboratory and 174 strains (53.2%) were serotyped. Four serotypes, sv. 5 (18.4%), sv 4 (16%), sv. 2 (9.2%) and sv. 13 (8%) were the most frequently isolated and 29.3% of the studied strains were classified as non typable. The results obtained indicate that the distribution of the serotypes in Spain is very similar to that found by other researchers in Germany, Australia, Canada and alike to that found in the United States.  相似文献   

Rural veterinary services in Western Australia: Part B. Rural practice   总被引：1，自引：1，他引：0  

Maxwell JA  Costa ND  Layman LL  Robertson ID 《Australian veterinary journal》2008,86(3):74-80

OBJECTIVE: To determine the current status of rural veterinary services in Western Australia. PROCEDURE: A questionnaire was sent to all eligible rural practitioners registered in 2006 and the replies were transferred to Microsoft Excel for analysis. RESULTS: Of the rural practitioners invited to participate in the survey replies were received from 67%. There were equal numbers of females and males. Their mean age was 44 years. Ninety per cent of respondents considered knowledge gained as an undergraduate was sufficient to equip them for practice, but only 60% considered their practical skills adequate. Thirteen per cent of those in rural practices in 2005 had left by 2006. Eighty-nine per cent of respondents were in mixed practice, the balance in specific species practice, such as equine, large animal and production animal consultancy. The majority of rural practitioners relied on servicing companion animals for their viability; 7% earned their income from servicing production animals only. Seventy per cent utilised merchandising and the sale of pet foods to supplement the income received from the traditional veterinary services and 34% found it necessary to earn an independent income. A quarter considered that rural practice did not have a future. CONCLUSION: The majority of rural practitioners in Western Australia depend on companion animals, not production animals, to remain viable, with very few operating production animal services. Poor remuneration is a major reason why veterinarians leave rural practice, and many find it necessary to supplement their income or develop an independent income.  相似文献   

A multicenter retrospective study on cryopreserved amniotic membrane transplantation for the treatment of complicated corneal ulcers in the dog     

Daniel Costa  Marta Leiva  Fernando Sanz  Victoria Espejo  Javier Esteban  Jorge Vergara  Clinio Díaz  Eduardo Huguet  Martí Cair  Jos Ríos  Maria Teresa Pea 《Veterinary ophthalmology》2019,22(5):695-702

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Genetic background of CTX‐M‐15‐producing Enterobacter hormaechei ST114 and Citrobacter freundii ST265 co‐infecting a free‐living green turtle (Chelonia mydas)     

Daphne W. Goldberg  Miriam R. Fernandes  Fbio P. Sellera  Daniela G. C. Costa  Ana Paula Loureiro Bracarense  Nilton Lincopan 《Zoonoses and public health》2019,66(5):540-545

CTX‐M‐type extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacteriaceae have become identified in marine ecosystem constituting a serious ecological issue. In this respect, although contamination of coastal waters and seafood, and even colonization of seabirds and fishes have been increasingly reported, molecular data are lacking to elucidate the clinical impact of ESBL producers in infected marine animals. In this study, using a genomic approach, we have analysed the genetic background of CTX‐M‐15‐producing Enterobacter hormaechei (belonging to the international human clone ST114) and Citrobacter freundii (ST265) co‐infecting a free‐living green turtle (Chelonia mydas) suffering from septic arthritis, which progressed to generalized coelomitis and death. Wide resistome of these pathogens contributed to treatment failure and death of the animal.  相似文献   

Strategies of the control of an outbreak of leptospiral infection in dairy cattle in Northeastern Brazil     

Pimenta  Carla Lauise Rodrigues Menezes  da Costa  Diego Figueiredo  Silva  Maria Luana Cristiny Rodrigues  Pereira  Hélio Domingos  Júnior  João Pessoa Araújo  Malossi  Camila Dantas  Ullmann  Leila Sabrina  Alves  Clebert José  de Azevedo  Sérgio Santos 《Tropical animal health and production》2019,51(1):237-241

The aim of the present study was to describe the strategies of the control of an outbreak of leptospiral infection in dairy cattle in Maranhão State, Northeastern Brazil. In the period from January to July 2015, 18 (17%) out of 106 cows presented abortion, six (5.7%) stillbirth, and 12 (11.3%) repeated estrus, totaling 24 animals with reproductive problems. The diagnosis of leptospirosis was based on serology (microscopic agglutination test—MAT), bacteriological culture, and polymerase chain reaction (PCR). Antibiotic therapy, vaccination protocols, and changes in management practices were suggested as control measures. Of all animals on the farm (n?=?280), 136 (48.6%) were seropositive for at least one serovar of Leptospira sp. No pure leptospiral culture was obtained. Eight of the animals with reproductive problems yielded positive PCR results (vaginal fluid of seven animals and urine and vaginal fluid of one animal). Genetic sequencing of a vaginal fluid/urine PCR-positive sample revealed Leptospira borgpetersenii. One year after the adoption of control measures, no reproductive problems were observed. Thus, leptospirosis probably caused the reproductive failures in the herd, and the control and prevention measures implemented were efficient in controlling the disease.

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Intravenous infusion of magnesium sulfate and its effect on horses with trigeminal‐mediated headshaking     

Shara A. Sheldon  Monica Aleman  Lais Rosa R. Costa  Ana C. Santoyo  Quinn Howey  John E. Madigan 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):923-932

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MicroRNA expression in the cerebrospinal fluid of dogs with and without cervical spondylomyelopathy     

Daniella P. Vansteenkiste  Joelle M. Fenger  Paolo Fadda  Paula Martin‐Vaquero  Ronaldo C. da Costa 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(6):2685-2692

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Supplementation of L‐glycine and L‐glutamate to Japanese quails from 01 to 36 days of age using the ideal protein concept     

Danilo V. G. Vieira  Jos H. V. Silva  Fernando G. P. Costa  Thiago S. Melo  Matheus R. Lima  Danilo T. Cavalcante  Jos G. de Vargas Júnior  Talita P. Bonaparte  Lorena C. Oliveira 《Journal of animal physiology and animal nutrition》2019,103(1):116-124

Experiment I: T_1‐1 = basal diet with 25% crude protein (CP) + limiting amino acids (LA); T_1‐2 = 20% CP + LA; T_1‐3 = 20% CP + LA + L‐glycine; T_1‐4 = 20% CP + LA + L‐glutamate; T_1‐5 = 20% CP + LA + L‐glycine + L‐glutamate. Experiment II: T_2‐1 = basal diet with 22% CP + LA; T_2‐2 = 20% CP + LA; T_2‐3 = 17.6% CP + LA + L‐glycine; T_2‐4 = 17.6% CP + LA + L‐glutamate; T_2‐5 = 17.6% CP + LA + L‐glycine + L‐glutamate. The reduction of dietary protein based on the concept of ideal protein decreases nitrogen excretion in quails when L‐glycine is added to the diets. Quails fed diets supplemented with L‐glutamate as the non‐specific nitrogen source equivalent to the nitrogen level of the control diet had increased nitrogen excretion. However, quails had reduced nitrogen excretion in both experiments when L‐glycine was added to diets with L‐glutamate. Carcass fat was increased by reducing dietary protein, but fat deposition was reduced by adding L‐glutamate and L‐glycine, or both. The dietary addition of L‐glutamate and L‐glycine in quails based on the ideal protein concept is not necessary (Exp. I). Although the total nitrogen, electrolytic balance and glycine level were adjusted in diets, quails had decreased performance. Therefore, other hypotheses besides protein reduction need to be studied (Exp. II). Protein reduction with supplementation of only limiting essential amino acids does not affect quail performance. Dietary addition of L‐glycine reduces nitrogen excretion.  相似文献   

Comparison of demographic data,disease severity and response to treatment,between dogs with atopic dermatitis and atopic‐like dermatitis: a retrospective study     

Larissa S. Botoni  Sheila M. F. Torres  Sandra N. Koch  Marcos B. Heinemann  Adriane P. Costa‐Val 《Veterinary dermatology》2019,30(1):10-e4

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Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos     

Luiz Sergio Almeida Camargo  Fernanda Queiros Costa  Michele Munk  Sabine Wohlres‐Viana  Raquel Varela Serapio  Bruno Campos Carvalho  Paulo Henrique Campos  Alex Cabral Vieira  Luiz Altamiro Garcia Nogueira  Joao Henrique Moreira Viana 《Reproduction in domestic animals》2019,54(10):1357-1365

This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus–oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleavage at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes.  相似文献