The combined effects of microbial insecticides and sodium chloride on the development and emergence of Chironomus xanthus     

Aline Silvestre Pereira Dornelas  João L.T. Pestana  Althiéris de Souza Saraiva  Rone Silva Barbosa  Grasiele Soares Cavallini  Carlos Gravato  Amadeu Mortágua Velho da Maia Soares  Renato Almeida Sarmento 《Pest management science》2023,79(6):2255-2263

  相似文献   

Systolic cardiac function assessment by feature tracking echocardiography in dogs with myxomatous mitral valve disease          下载免费PDF全文

M. M. Mantovani  R. A. L. Muzzi  G. G. Pereira  R. J. Yamato  A. C. Silva  G. F. Reis  L. A. L. Muzzi  E. C. Guimarães 《The Journal of small animal practice》2015,56(6):383-392

  相似文献   

Two Methods of Vitrification Followed by In Vitro Culture of the Ovine Ovary: Evaluation of the Follicular Development and Ovarian Extracellular Matrix          下载免费PDF全文

FT Bandeira  AA Carvalho  SV Castro  LF Lima  DA Viana  JSAM Evangelista  MJS Pereira  CC Campello  JR Figueiredo  APR Rodrigues 《Reproduction in domestic animals》2015,50(2):177-185

The aim of this study was to evaluate the influence of two vitrification techniques on the extra cellular matrix (ECM) and ovarian follicular development. The ovarian cortex was fragmented (9 mm³) and divided into six groups, viz. fresh control, cultured control, vitrified by the Ovarian Tissue Cryosystem (OTC) method, conventional solid surface vitrification (SSV) method, OTC/cultured and SSV/cultured. Follicles from all the fragments were analysed for morphology, development and viability. The ECM was evaluated based on the condition of collagen and reticular fibres and the immunolocalization of type I collagen and fibronectin. After 7 days of culture, the tissue vitrified by OTC revealed a higher percentage (p < 0.05) of morphologically normal (30.66%) and viable (60.00%) follicles when compared with those vitrified using the SSV technique (21.33% and 23.00%). In all the fragments cultured, regardless of the vitrification method, a significantly higher percentage of developing follicles was observed when compared with the non‐cultured tissue. Analysis of the type I collagen showed increased immunostaining after the in vitro culture in the vitrified fragments. In conclusion, the OTC is better for preserving the follicular viability and morphology and maintaining the integrity of the extracellular matrix components of the ovine ovary.  相似文献   

Productivity,nutrient immobilization and soil chemical properties in anEucalyptus globulus plantation under different irrigation and fertilization regimes     

M. Madeira  J. S. Pereira 《Water, air, and soil pollution》1990,54(4):621-634

To evaluate the influence of irrigation and fertilization on the productivity ofE. globulus a field experiment was started in 1986 in Central Portugal. The nutrients accumulated in the biomass and their allocation to the various biomass components as well as the changes in soil chemical characteristics were also followed. Irrigation and fertilization resulted in a significant increase of biomass production during the first 2 yr. A similar trend occurred with the net primary production and leaf Litterfall. The differences between treatments in the amounts of accumulated nutrients in the aboveground biomass were similar to those of biomass accumulation. However, there was a small but consistent effect related to the average concentration of nutrients in the plant tissues. This effect occurred essentially in the leaves and branches. The concentration of N and P in the leaf Litterfall was higher in the fertilized treatments than in the others. Fertilization alone induced a slight increase in the soil pH values, C content, exchangeable Ca and available P levels. Such increase was more pronounced in the fertilized with dripping irrigation treatment. This was due to the accumulation of nutrients in the wetted soil volume.  相似文献   

Candidate genes for growth traits in beef cattle crosses Bos taurus × Bos indicus     

D. D. Tambasco  C. C. P. Paz  M. Tambasco-Studart  A. P. Pereira  M. M. Alencar  A. R. Freitas  L. L. Coutinho  I. U. Packer  L. C. A. Regitano 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2003,120(1):51-56

  相似文献   

Presence of Grapevine leafroll-associated virus 3 in primary salivary glands of the mealybug vector Planococcus citri suggests a circulative transmission mechanism     

Miguel Cid  Sonia Pereira  Cristina Cabaleiro  Franco Faoro  Antonio Segura 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(1):23-30

Grapevine leafroll-associated virus 3 (GLRaV-3) is a mealybug-transmissible ampelovirus. Though the transmission mechanism has been described as semipersistent on the basis of temporal parameters, definitive proof of this mechanism has never been provided. In the present study, we carried out preliminary assays to establish the location of the virus in its vector, Planococcus citri. After dissecting the insects, GLRaV-3 was detected by means of IC-RT-PCR in the salivary glands, intestine and Malpighian tubes, but not in the sucking apparatus. Immunogold labelling of the capsid protein revealed the presence of the virus in some cells of the primary salivary glands, but not in the alimentary channel of the stylet, or in the accessory salivary glands. The strong labelling of the electron-dense secretion vesicles in some cells of the primary salivary glands, together with the non-detection of the virus in the sucking apparatus suggests that the transmission mechanism may be different from that previously described. We propose a circulative transmission mechanism based on a specific transportation route for the viral particles from the midgut or hindgut to the salivary glands. As the transmission mechanism is generally a common feature of a viral genus, the existence of a circulative transmission mechanism for other mealybug-transmitted ampeloviruses is expected. Organ by organ analysis of GLRaV-1, another ampelovirus not transmissible by P. citri, showed the absence of the virus in the salivary glands, thus providing further, though indirect, evidence in favour of circulative transmission for this virus genus.  相似文献   

A two generation chronic mixture toxicity study of Clophen A60 and diethyl phthalate after gestational and lactational exposure in female Wistar rats     

Contzen Pereira  C. Vaman Rao 《Pesticide biochemistry and physiology》2007,88(2):156-166

Polychlorinated biphenyls and diethyl phthalate are both lipophilic in nature and are likely to be present in the same environmental compartment or bioaccumalate over a period of time, thus a mixture toxicity study was undertaken to evaluate the type of interaction between polychlorinated biphenyls (Clophen A60) and diethyl phthalate over two generations in female Wistar rats. Healthy male and female albino rats of Wistar strain weighing 75-100 g (6-7 weeks old) were randomly assigned to four groups of six each. Group I male and female rats were fed on normal diet and water ad libitum. Group II male and female rats were maintained on normal diet mixed with corn oil as oil control. GroupS III and IV male and female rats were given Clophen A60 and diethyl phthalate dissolved in corn oil mixed with the diet at 50 mg/kg of the diet individually to each group. Group V male and female rats received a mixture of diethyl phthalate and Clophen A60, each dissolved in corn oil mixed with the diet at 50 mg/kg of the diet. Hundred days after the treatment, females were mated with the males in each group for 10 days. Exposure to diethyl phthalate and Clophen A60 was continued throughout mating, gestation until termination at weaning, which was 150 days of total treatment period of the parental generation female rats. Treatment for F1 generation male and female pups (6 males & 6 females) with Clophen A60 and diethyl phthalate individually and in mixture was continued at doses reduced to 25 mg/kg of the diet after they reached 75-100 g in weight. The treatment was carried out similar to the parental generation for a period of 150 days. Liver and serum aspartate aminotransferase, liver cholesterol and glycogen were significantly increased in the F1 generation Clophen A60 + diethyl phthalate treated group, whereas serum cholesterol, liver glutathione and glutathione reductase showed a significant decrease in the F1 generation Clophen A60 + diethyl phthalate treated group as compared to the parental generation mixture and individually treated groups as well as the individually treated F1 generation groups. A significant increase was observed in the liver and serum aspartate aminotransferase activity of Clophen A60 and serum aspartate aminotransferase levels of diethyl phthalate treated F1 generation rats as compared to the parental generation Clophen A60 and diethyl phthalate individually and mixture treated rats. Liver glutathione levels were significantly decreased in the F1 generation Clophen A60 and diethyl phthalate individually treated rats which was similar to the parental generation individually treated rats as compared to the controls. Liver glutathione reductase level was also significantly declined in the diethyl phthalate treated F1 individual group as compared to diethyl phthalate individually treated parental generation rats. Histology of the liver showed fatty degeneration in the mixture treated F1 generation rats as compared to Clophen A60 and diethyl phthalate individually treated F1 rats and parental generation Clophen A60 and diethyl phthalate individually and mixture treated rats. Thus, in spite of dose reduction and continuous exposure over two generation’s to a mixture of diethyl phthalate and Clophen A60 exposed through gestation, lactation and diet leads to a synergistic toxic effect in the F1 generation.  相似文献   

First report of Sphenospora kevorkianii (Raveneliaceae) on the orchid Catasetum fimbriatum in Brazil     

O. L. Pereira  R. W. Barreto 《Plant pathology》2004,53(2):256-256

  相似文献   

Towards the isolation of resistance genes by transposon targeting in potato     

A. Pereira  J. M. E. Jacobs  W. Te Lintel-Hekkert  E. Rutgers  E. Jacobsen  W. J. Stiekema 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(Z2):215-221

A general strategy for the isolation of disease resistance genes is presented, employing a two-step approach of transposon targeting near genes of interest followed by transposon tagging. A library of transposon (Ac/Ds) transformants in a self fertile potato diploid are being mapped by deriving genomic DNA probes flanking the transposon containing T-DNA insertions with the inverse polymerase chain reaction and using these probes for RFLP analysis. We have produced a large number of transposon (Ac/Ds) transformants in a self fertile potato diploid. Genomic DNA probes, flanking the transposon containing T-DNA insertions, are produced by the inverse polymerase chain reaction (IPCR) and mapped by restriction fragment length polymorphism (RFLP) analysis in a segragating potato location. A transposon mapped close to a resistance gene can be recombined cis to the gene and used for efficient transposon targeting due to preferential transposition to linked sites.  相似文献   

A comparison of potato seed tuber yields in beds,pots and hydroponic systems     

Ricardo Monteiro Corrêa  José Eduardo Brasil Pereira Pinto  César Augusto Brasil Pereira Pinto  Valdemar Faquin  Érika Soares Reis  Aline Beraldo Monteiro  Willian E. Dyer 《Scientia Horticulturae》2008

The objective of this study was to compare potato seed tuber production of cvs. Monalisa and Agata growing in beds, pots or hydroponics, with either single or staggered harvests. All culture systems were established in plastic sheeting-covered greenhouses protected with an anti-aphid network. The beds and 3 L pots were filled with Plantmax^® substrate and placed in suspended beds. The hydroponic system utilized NFT (Nutrient film technique) and 4 m × 15 cm × 7 cm PVC tubes with a 4% slope and the fertilizers were a commercial formula. Each experiment was 4 treatments in factorial Scheme 2 × 2 with 2 cultivars (Monalisa and Agata) and 2 harvest methods (single and staggered). All three experiments were carried out in randomized design with 6 replicates and 7 plants per replicate.  相似文献