The rotavirus genus     

Gianfranco Donelli  Fabiana Superti 《Comparative immunology, microbiology and infectious diseases》1994,17(3-4):305-320

Human rotaviruses, discovered nearly 20 years ago, have been proven to be major cause of paediatric diarrhoeal disease morbidity and mortality. The clinical significance of these viruses stimulated basic studies on their biology, molecular and antigenic properties and epidemiology. General features, clinical relevance, epidemiologic pattern and laboratory diagnosis of human rotavirus infections are here reviewed.  相似文献   

Prognosis of aboveground woody biomass in a central Brazilian Cerrado monitored for 27 years after the implementation of management systems     

de Azevedo  Gileno Brito  Rezende  Alba Valéria  Azevedo  Glauce Taís de Oliveira Sousa  Miguel  Eder Pereira  Aquino  Fabiana de Gois  Teodoro  Larissa Pereira Ribeiro  Teodoro  Paulo Eduardo 《European Journal of Forest Research》2022,141(1):1-15

Accurate estimation of biomass in natural vegetation sites remains a challenge. Modeling biomass growth and production in Cerrado areas is crucial to understanding the vegetation succession process, especially regarding the changes in biomass accumulation over time. Thus, our objective was to model the growth and production of woody aboveground biomass (living and total) in a cerrado stricto sensu monitored for 27 years after implementing management systems. As expected, the basal area (with a diameter taken at 30 cm from the ground level) is the most important predictor variable and showed a higher correlation with the biomass stocks and allowed accurate and consistent estimates of these accumulated stocks over time. Future estimates of biomass production, generated from growth models that estimate production as a function of parameters observed at previous ages, indicate that maximum stocks of living (25.86?±?0.15 Mg ha^?1 [mean?±?standard deviation]) and total aboveground biomass (26.11?±?0.15 Mg ha^?1) are expected for a period between 28 and 30 years after the implementation of the management systems, with maximum mean annual increment between 23 and 27 years. Furthermore, the systems of equations obtained simulated reductions up to 30% of biomass after the occurrence of a forest fire at 23 years. Thus, our study can be useful for the decision-making process and developing public policies and strategies for managing and conserving natural resources in the Cerrado biome.

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Soil sulfur fractions dynamics and distribution in a tropical grass pasture amended with nitrogen and sulfur fertilizers     

Fabiana Schmidt  Fabiano Daniel De Bona  Cristiane Prezotto Silveira  Francisco Antonio Monteiro 《植物养料与土壤学杂志》2012,175(1):60-67

Soil sulfur (S) partitioning among the various pools and changes in tropical pasture ecosystems remain poorly understood. Our study aimed to investigate the dynamics and distribution of soil S fractions in an 8‐year‐old signal grass (Brachiaria decumbens Stapf.) pasture fertilized with nitrogen (N) and S. A factorial combination of two N rates (0 and 600 kg N ha^–1 y^–1, as NH₄NO₃) and two S rates (0 and 60 kg S ha^–1 y^–1, as gypsum) were applied to signal grass pastures during 2 y. Cattle grazing was controlled during the experimental period. Organic S was the major S pool found in the tropical pasture soil, and represented 97% to 99% of total S content. Among the organic S fractions, residual S was the most abundant (42% to 67% of total S), followed by ester‐bonded S (19% to 42%), and C‐bonded S (11% to 19%). Plant‐available inorganic SO₄‐S concentrations were very low, even for the treatments receiving S fertilizers. Low inorganic SO₄‐S stocks suggest that S losses may play a major role in S dynamics of sandy tropical soils. Nitrogen and S additions affected forage yield, S plant uptake, and organic S fractions in the soil. Among the various soil fractions, residual S showed the greatest changes in response to N and S fertilization. Soil organic S increased in plots fertilized with S following the residual S fraction increment (16.6% to 34.8%). Soils cultivated without N and S fertilization showed a decrease in all soil organic S fractions.  相似文献   

Effect of industrial processing on the distribution of aflatoxins and zearalenone in corn-milling fractions     

Brera C  Catano C  de Santis B  Debegnach F  de Giacomo M  Pannunzi E  Miraglia M 《Journal of agricultural and food chemistry》2006,54(14):5014-5019

The aim of this study was to investigate the distribution of aflatoxins and zearalenone levels in various corn-milling fractions. Corn kernels and six derived milling fractions (germ, bran, large and small grits, flour, and animal feed flour) were sampled in an industrial plant; both conventional and organic corns were sampled. To evaluate the effect of cooking, samples of polenta were prepared starting from naturally contaminated flour. Conventional and organic lots showed mycotoxin contamination. For both lots, germ, bran, and animal feed flour showed a marked concentration factor from 239 to 911% accounting for both the low yields of the derived products and the distribution of aflatoxins and zearalenone contamination in the outer parts of the kernels. Conversely, a reduction factor of at least four times from raw material to finished products was observed. Polenta samples were unaffected by the cooking process, with levels of contamination similar to those of starting flour.  相似文献   

PAF increases phagocytic capacity and superoxide anion production in equine alveolar macrophages and blood neutrophils     

Muehlmann LA  Michelotto PV  Nunes EA  Grando FC  da Silva FT  Nishiyama A 《Research in veterinary science》2012,93(1):393-397

Phagocytosis exerted by alveolar macrophages and neutrophils is crucial in the clearance of exogenous particles deposited in the airways. Therefore, substances that activate these phagocytes in the airways can exert important effects on the particle clearance rate. PAF, particularly, was proved to be a potent activator of several immune cells and was shown to be present in the equine lower airways in specific conditions, such as after exercise. The present study aimed to investigate if PAF is able to increase the phagocytic capacity and the production of superoxide anion in equine alveolar macrophage and blood neutrophils. The results show that PAF increased these parameters in both phagocytes even in concentrations as low as 0.1 and 1.0 nM. On that ground, the present work suggests that PAF is involved in the process of particle clearance in equine lower airways.  相似文献   

Comparison of cellular location and expression of Plakophilin‐2 in epidermal cells from nonlesional atopic skin and healthy skin in German shepherd dogs          下载免费PDF全文

Brita Ardesjö‐Lundgren  Katarina Tengvall  Kerstin Bergvall  Fabiana H.G. Farias  Liya Wang  Åke Hedhammar  Kerstin Lindblad‐Toh  Göran Andersson 《Veterinary dermatology》2017,28(4):377-e88

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Antiviral efficacy of EICAR against canine distemper virus (CDV) in vitro     

Fabiana Dal Pozzo 《Research in veterinary science》2010,88(2):339-344

Canine distemper virus (CDV) is a highly contagious pathogen of carnivores. In dogs, the disease is characterized by high lethality rates and no specific antiviral therapy is available. The aim of this study was to verify the in vitro antiviral activity of the 5-ethynyl-1-β-d-ribofuranosylimidazole-4-carboxamide (EICAR) and to compare it with the 1-(β-d-ribofuranosyl)-1,2,4-triazole-3-carboxamide (ribavirin, RBV).EICAR was more active than RBV against CDV replication, while both molecules exhibited low selectivity indexes. A reversal of their antiviral activity was observed after addition of guanosine, suggesting their involvement in the inhibition of the inosine monophosphate dehydrogenase enzyme (IMPDH). RBV and EICAR had a time- and concentration-dependent anti-CDV activity, mainly displayed during the first 10 h post-infection. The involvement of the inhibition of the viral RNA-dependent RNA polymerase (vRdRp) is discussed, as well as the role of CDV as a model to study more potent and selective antiviral molecules active against other Paramyxoviridae.  相似文献   

Modelling the distribution of marine fishery resources: Where are we?     

Lucas dos Santos Rodrigues  Maria Grazia Pennino  David Conesa  Eidi Kikuchi  Paul Gerhard Kinas  Fabiana Gonçalves Barbosa  Luís Gustavo Cardoso 《Fish and Fisheries》2023,24(1):159-175

Ecological niche models (ENMs) and species distribution models (SDMs) have been widely applied to various studies relevant to biogeography, conservation biology, and ecology. These modelling techniques seek to develop spatial maps for projecting, among others past, current, and future species distributions. Born in the field of terrestrial ecology, only in recent years have these models been applied to marine environmental issues, especially to improve the forecasting of the distribution of occurrences and capturing of fishery resources. This study aimed to present through bibliometric analysis the characteristics of articles related to the use of ENMs and SDMs in marine fishery resources considering three main points: (1) state of the art: number of articles over the years, journals, countries, collaborations, and focus of research; (2) characteristics linked to fishery resources: marine biogeographic realms, taxonomic groups, life phases, oceanographic zones, and behaviours; (3) characteristics linked to methods: type of method, type of biological and, environmental data. We provide a list of 378 articles (derived from 930 screened ones), the results, and a discussion of our findings, which represent a baseline for the current status (strengths, limits, and gaps) of the interface between ENMs/SDMs and fishery resources.  相似文献   

Practical diets with essential oils of plants activate the complement system and alter the intestinal morphology of Nile tilapia          下载免费PDF全文

Gustavo M. R. Valladão  Sílvia U. Gallani  Gabriela Pala  Raphael B. Jesus  Suzana Kotzent  Jaqueline C. Costa  Thiago F. A. Silva  Fabiana Pilarski 《Aquaculture Research》2017,48(11):5640-5649

The effect of the essential oils (EOs) of peppermint, Mentha piperita L., and tea tree, Melaleuca alternifolia (Maiden & Betche) Cheel, on the haematological, biochemical, and immunological parameters and intestinal morphology of Nile tilapia, Oreochromis niloticus L., was evaluated. Fish (58.09 ± 5.87 g) were fed 100 mg/kg and 250 mg/kg of each EO and sampled on days 7, 14, 30 and 60 after starting supplementation. The haematological and biochemical parameters were not altered by the supplementation of EOs compared to the control (p > .05). With regard to the immunological parameters, the activation of the complement system of fish fed 250 mg/kg peppermint and 100 mg/kg and 250 mg/kg tea tree EOs were significantly higher compared to the control after 60 days of feeding (p < .05). The complement system plays an essential role in innate immunity and contributes significantly to the acquired immune response; thus, its activation through supplementation with EOs is promising for the formulation of nutritional additives in aquaculture. Regarding intestinal morphology, fish fed 250 mg/kg tea tree EO presented higher villus size compared to all other groups (p < .05), which represents a healthier gut. These fish present a larger intestinal surface, which can result in better absorption and utilization of the nutrients. Based on the responses found in this study, both EOs were considered promising for the formulation of feed additives for Nile tilapia.  相似文献   

Genetic and biologic characteristics of Toxoplasma gondii isolates in free-range chickens from Colombia, South America   总被引：3，自引：0，他引：3  

Dubey JP  Gomez-Marin JE  Bedoya A  Lora F  Vianna MC  Hill D  Kwok OC  Shen SK  Marcet PL  Lehmann T 《Veterinary parasitology》2005,134(1-2):67-72

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 77 free-range chickens (Gallus domesticus) from Colombia, South America was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT), and found in 32 (44.4%) of 72 chickens with titers of 1:5 in 4, 1:10 in 3, 1:20 in 1, 1:40 in 1, 1:80 in 8, 1:160 in 8, 1:320 in 3, and 1:640 or higher in 4. Hearts and brains of 31 seropositive chickens were pooled and bioassayed in mice. Tissues from 32 (16+16) seronegative chickens were pooled and fed to two, T. gondii-free cats, and tissues from nine chickens without matching sera were fed to one T. gondii-free cat. Feces of cats were examined for oocysts. T. gondii oocysts were excreted by a cat that was fed tissues of 16 seronegative chickens. T. gondii was isolated by bioassay in mice from 23 chickens with MAT titers of 1:20 or higher. All infected mice from 16 of the 23 isolates died of toxoplasmosis. Overall, 82 (81.1%) of 101 mice that became infected after inoculation with chicken tissues died of toxoplasmosis. Genotyping of these 24 isolates using polymorphisms at the SAG2 locus indicated that seven T. gondii isolates were Type I, 17 were Type III, and none was Type II. Phenotypically, T. gondii isolates from chickens from Colombia were similar to isolates from Brazil but different from the isolates from North America; most isolates from chickens from Brazil and Colombia were lethal for mice whereas isolates from North America did not kill inoculated mice. Genetically, none of the T. gondii isolates from Colombia and Brazil was SAG2 Type II, whereas most isolates from chickens from North America were Type II. This is the first report of genetic characterization of T. gondii isolates from Colombia, South America.  相似文献