Studies on the constituents of a Brazilian folk infusion. Isolation and structure elucidation of new triterpene saponins from Ilex amara leaves.     

Fabio Donisete Pezzuto de Andrade  Sonia Piacente  Cosimo Pizza  Wagner Vilegas 《Journal of agricultural and food chemistry》2002,50(2):255-261

The isolation of three new triterpene saponins 3beta-O-beta-D-glucopyranosyl-(1-->3)-alpha-L-2-O-acetylarabinopyranosylolean-12-en-28-oic acid 28-O-beta-D-glucopyranosyl ester (2), 3beta-O-beta-D-glucopyranosyl-(1-->2)-alpha-L-O-arabinopyranosylurs-12-en-28-oic acid (3), and 3beta-O-beta-D-glucopyranosyl-(1-->2)-beta-D-O-galactopyranosylurs-12-en-28-oic acid (4) together with five known saponins and one flavonoid glycoside from the aqueous infusion of Ilex amara (Vellozo) Loes. leaves is reported. All structures were elucidated by spectroscopic methods, including the concerted application of one-dimensional (1H, TOCSY, 13C, and 13C DEPT NMR) and two-dimensional NMR techniques (DQF-COSY, HSQC, and HMBC).  相似文献   

Quantification of the biocontrol agent Pseudomonas fluorescens Pf153 in soil using a quantitative competitive PCR assay unaffected by variability in cell lysis- and DNA-extraction efficiency     

Davide Gobbin  Fabio Rezzonico  Cesare Gessler 《Soil biology & biochemistry》2007,39(7):1609-1619

Although often neglected, variability in cell lysis efficiency and DNA extraction yield represents the major hurdles of any polymerase chain reaction (PCR)-based quantification protocol in soil and other natural environments. In this study we developed a technique that minimizes the effects of these constraints, providing at the same time a reliable internal control to distinguish between PCR-inhibition and negative results. We used Pseudomonas fluorescens Pf153, a root-colonizing bacterium that shows biocontrol activity against tobacco and cucumber black root rot, as the target organism for PCR quantification. Prior to DNA extraction, the genetically engineered, cognate reference strain P. fluorescens CHA0/c2 was inoculated in a reference soil. CHA0/c2 in the reference soil and Pf153 in the soil sample were lysed in parallel and afterward the lysates were mixed in known proportions. CHA0/c2 carries the plasmid pME6031-cmp2 that contains an allelic variant (competitor) of the Pf153 specific sequence Pf153_2. In a quantitative competitive PCR (QC-PCR) assay the competitor allows the quantification of the target strain down to 0.66 Pf153 CFU/mg soil. Processing the reference strain in the same way as Pf153 enables the exact quantification of the target strain in biocontrol assays performed in natural soil, overcoming differences in DNA extraction efficiency and PCR amplification from different soil environments. This technique is easily adaptable to other Pseudomonas strains simply by replacing the competitor used here with one derived from a SCAR-marker which is specific for the strain of choice.  相似文献   

SOIL STRUCTURE IN VERTISOLS OF THE BLUE NILE CLAY PLAINS, SUDAN     

J. H. DE  VOS t. N. C. K. J. VIRGO 《European Journal of Soil Science》1969,20(1):189-206

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A map of the interactome network of the metazoan C. elegans   总被引：1，自引：0，他引：1  

Li S  Armstrong CM  Bertin N  Ge H  Milstein S  Boxem M  Vidalain PO  Han JD  Chesneau A  Hao T  Goldberg DS  Li N  Martinez M  Rual JF  Lamesch P  Xu L  Tewari M  Wong SL  Zhang LV  Berriz GF  Jacotot L  Vaglio P  Reboul J  Hirozane-Kishikawa T  Li Q  Gabel HW  Elewa A  Baumgartner B  Rose DJ  Yu H  Bosak S  Sequerra R  Fraser A  Mango SE  Saxton WM  Strome S  Van Den Heuvel S  Piano F  Vandenhaute J  Sardet C  Gerstein M  Doucette-Stamm L  Gunsalus KC  Harper JW  Cusick ME  Roth FP  Hill DE  Vidal M 《Science (New York, N.Y.)》2004,303(5657):540-543

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Comparative Earth History and Late Permian Mass Extinction   总被引：6，自引：0，他引：6  

AH Knoll  RK Bambach  DE Canfield  JP Grotzinger 《Science (New York, N.Y.)》1996,273(5274):452-457

The repeated association during the late Neoproterozoic Era of large carbon-isotopic excursions, continental glaciation, and stratigraphically anomalous carbonate precipitation provides a framework for interpreting the reprise of these conditions on the Late Permian Earth. A paleoceanographic model that was developed to explain these stratigraphically linked phenomena suggests that the overturn of anoxic deep oceans during the Late Permian introduced high concentrations of carbon dioxide into surficial environments. The predicted physiological and climatic consequences for marine and terrestrial organisms are in good accord with the observed timing and selectivity of Late Permian mass extinction.  相似文献   

Magnetotactic bacteria at the geomagnetic equator     

Frankel RB  Blakemore RP  DE Araujo FF  Esquivel DM  Danon J 《Science (New York, N.Y.)》1981,212(4500):1269-1270

Magnetotactic bacteria are present in fresh water and marine sediments of Fortaleza, Brazil, situated close to the geomagnetic equator. Both South-seeking and North-seeking bacteria are present in roughly equal numbers in the same samples. This observation is consistent with the hypothesis that the vertical component of the geomagnetic field selects the predominant polarity type among magnetotactic bacteria in natural environments.  相似文献   

NUTRITIVE VALUE OF THE MEXICAN TORTILLA     

Cravioto RO  Anderson RK  Lockhart EE  DE P Miranda F  Harris RS 《Science (New York, N.Y.)》1945,102(2639):91-93

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The Name of America     

DE St Bris T 《Science (New York, N.Y.)》1888,12(283):12

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STUDIES ON THE FATE OF PLASMA PROTHROMBIN     

DE W Andrus W  Lord JW  Kauer JT 《Science (New York, N.Y.)》1940,91(2350):48-50

The level of the plasma prothrombin in the circulating blood is decreased during its passage through the pulmonary capillaries. In 85 per cent. of samples, plasma prothrombin has been found to be less in the blood of the left ventricle than in that from the right ventricle. This difference averaged 10.6 per cent. and ranged from 4 to 19 per cent. In no instance was the level in the right ventricle lower than that in the left. In samples of blood taken from the arterial and venous supply of the head, liver, spleen, intestine, kidney and hind limbs, no significant difference in plasma prothrombin levels was found. A possible explanation of this role of the lung in the loss of plasma prothrombin is thought to be the production of blood platelets in this organ, as demonstrated by Howell and Donahue. Platelets, as they undergo disintegration, initiate the first stage of the clotting process by releasing thromboplastin, which, in the presence of calcium, changes prothrombin to thrombin.  相似文献   

Racemization of Amino Acids in Dipeptides Shows COOH > NH2 for Non-Sterically Hindered Residues     

Smith GG  DE Sol BS 《Science (New York, N.Y.)》1980,207(4432):765-767

The relative rates of racemization for amino acid residues at the NH(2) and COOH ends of 37 different dipeptides were determined. In nine dipeptides containing alanine, leucine, phenylalanine, aspartic acid, and methionine, the amino acid residue racemized faster at the COOH-terminal position than at the NH(2)-terminal position (COOH > NH(2)). The sterically hindered amino acids isoleucine and valine showed NH(2) > COOH. Six proline dipeptides showed NH(2) > COOH. Intramolecular effects have been invoked to explain these surprising results.  相似文献