Transplacental Transfer of Iron in the Water Buffalo (Bubalus bubalis): Uteroferrin and Erythrophagocytosis     

FTV Pereira  FC Braga  KC Burioli  JR Kfoury Jr  LJ Oliveira  PC Papa  AF Carvalho  CE Ambrósio  FW Bazer  MA Miglino 《Reproduction in domestic animals》2010,45(5):907-914

The objectives of this investigation were to understand transplacental transport of iron by secreted uteroferrin (UF) and haemophagous areas of water buffalo placenta and clarify the role(s) of blood extravasation at the placental‐maternal interface. Placentomes and interplacentomal region of 51 placentae at various stages of gestation were fixed, processed for light and transmission electron microscopy, histochemistry and immunohistochemistry. Haemophagous areas were present in placentomes collected between 4 and 10 months of pregnancy. Perl’s reaction for ferric iron was negative in placentomes, but positive in endometrial glands. Positive staining for UF indicated areas in which it was being taken up by phagocytosis and/or fluid phase pinocytosis in areolae of the interplacentomal mesenchyme, with little staining in endometrial stroma. Imunohistochemistry detected UF in trophectoderm of haemophagous regions of placentomes and in other parts of the foetal villous tree, but the strongest immunostaining was in the epithelial cells and lumen of uterine glands. Ultrastructural analyses indicated that erythrophagocytosis was occurring and that erythrocytes were present inside cells of the chorion that also contained endocytic vesicles and caveolae. Results of this study indicate that both the haemophagous areas of placentomes and the areolae at the interface between chorion and endometrial glands are important sites for iron transfer from mother to foetal‐placental tissues in buffalo throughout pregnancy.  相似文献   

Shipboard geophysical indications of asymmetry and melt production beneath the east pacific rise near the MELT experiment     

DS Scheirer  DW Forsyth  MH Cormier  KC Macdonald 《Science (New York, N.Y.)》1998,280(5367):1221-1224

Near the Mantle Electromagnetic and Tomography (MELT) Experiment, seamounts form and off-axis lava flows occur in a zone that extends farther to the west of the East Pacific Rise than to the east, indicating a broad, asymmetric region of melt production. More seamounts, slower subsidence, and less dense mantle on the western flank suggest transport of hotter mantle toward the axis from the west. Variations in axial ridge shape, axial magma chamber continuity, off-axis volcanism, and apparent mantle density indicate that upwelling is probably faster and more melt is produced beneath 17 degrees15'S than beneath 15 degrees55'S. Recent volcanism occurs above mantle with the lowest seismic velocities.  相似文献   

Therapeutic efficacy of zeta-cypermethrin pour-on for the treatment of biting and sucking lice in cattle under field conditions     

JT ROTHWELL  KC HACKET  I RIDLEY  L MITCHELL  C DONALDSON  LB LOWE 《Australian veterinary journal》1999,77(4):255-258

OBJECTIVE: To assess the efficacy of zeta-cypermethrin pour-on to control cattle lice. DESIGN: Five field trials in south-eastern Australia. PROCEDURE: Zeta-cypermethrin pour-on, deltamethrin pour-on and pour-on vehicle were applied to groups of 10 cattle. Lice were counted before treatment and 14, 28, 42 and 56 days after treatment. RESULTS: Zeta-cypermethrin pour-on given at 2.5 mg/kg was equivalent to, or marginally more effective than a deltamethrin pour-on at 0.75 mg/kg. It eliminated B bovis and H eurysternus and gave good control of L vituli and S capillatus. Zeta-cypermethrin at 1 mg/kg gave good control of B bovis and H eurysternus but was not satisfactory against L vituli and S capillatus. CONCLUSION: Zeta-cypermethrin pour-on, given at 2.5 mg/kg, is an effective treatment for cattle lice control. Zeta-cypermethrin, and other synthetic pyrethroid pour-ons, are the treatment of choice to control B bovis.  相似文献   

Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues   总被引：2，自引：0，他引：2  

Anna Lytovchenko  Romina Beleggia  Nicolas Schauer  Tal Isaacson  Jan E Leuendorf  Hanjo Hellmann  Jocelyn KC Rose  Alisdair R Fernie 《Plant methods》2009,5(1):4-11

Background  

The concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and experiments carried out. However, studies are generally confined to polar compounds alone. Here we describe a simple method for lipophilic compounds analysis in various plant tissues.  相似文献   

Adverse reaction to procaine penicillin G in horses     

IL NIELSEN  KA JACOBS  PJ HUNTINGTON†  CB CHAPMAN‡  KC LLOYD§ 《Australian veterinary journal》1988,65(6):181-185

Adverse reactions to intramuscular injections of procaine penicillin G are reported in 11 horses, five of which died. The clinical findings are presented and suggest central nervous involvement in most cases. Post mortem findings in one horse were consistent with anaphylaxis whereas in other cases the clinical findings, duration of treatment, speed of onset and subsequent completion of treatment supports diagnosis of an acute procaine toxicity syndrome.  相似文献   

Where are the jobs?     

Fanning O 《Science (New York, N.Y.)》1969,166(3912):1463

  相似文献   

A study of dendritic cell and MHC class II expression in dogs with immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis     

Breathnach RM  Fanning S  Mulcahy G  Bassett HF  Jones BR 《Veterinary journal (London, England : 1997)》2008,177(3):352-359

The term immunomodulatory-responsive lymphocytic-plasmacytic pododermatitis (ImR-LPP) has previously been proposed to denote a sub-population of dogs with idiopathic pododermatitis. The objective of this study was to investigate dendritic cell (DC) and MHC class II antigen expression in lesional skin of dogs with ImR-LPP (n = 47). Median epidermal CD1c⁺ cell counts were 37.8 and 12.5 mm⁻¹ in ImR-LPP dogs and healthy controls (n = 27), respectively (P < 0.01), while the corresponding dermal cell counts were 180.9 and 45.0 mm⁻², respectively (P < 0.01).Intra-epidermal clusters of DCs were observed in 18/47 dogs with ImR-LPP. Median epidermal MHC class II⁺ cell counts were 32.5 and 10.5 mm⁻¹ in ImR-LPP dogs and healthy controls, respectively (P < 0.01), while the corresponding dermal cell counts were 216.9 and 46.9 mm⁻², respectively (P < 0.01). Dermal MHC class II⁺ staining was primarily associated with DCs (47/47 dogs), mononuclear inflammatory cells (45/47), fibroblast-like cells (19/47) and vascular endothelium (14/47). The DC hyperplasia and increased MHC class II expression in lesional ImR-LPP skin are consistent with enhanced antigen presentation, and suggest that both parameters may contribute to the pathogenesis of ImR-LPP through the priming and activation of CD4⁺ T cells. Equally, it is possible that the enhanced DC numbers observed in this study may contribute to the immunoregulation of steady-state pathology in lesional ImR-LPP skin through additional expanded, although as yet unresolved, mechanisms.  相似文献   

Detection and characterisation of bovine rotavirus in Ireland from 2006–2008     

PJ Collins  Emily Mulherin  Olivia Cashman  Grainne Lennon  Lynda Gunn  Helen O’Shea  Séamus Fanning 《Irish veterinary journal》2014,67(1):13

Background

Worldwide, Group A bovine rotavirus (RVA boRV) is one of the main causes of neonatal calf diarrhoea. Currently, limited epidemiological and sequence data exists on the RVA disease in bovines in Southern Ireland only. The aim of the study was to generate epidemiological and sequence data of RVA boRV distributed over a wide geographical area in Ireland.

Findings

272 stool samples were obtained from symptomatic calves and analysed to identify the prevalent G and P genotypes. Viral type combinations including G6P[5], G6P[11] and G10P[11] genotype were the most frequently identified. The G6P[5] combination was predominant throughtout the study, accounting for 70% (n = 191). Sequence analysis of the VP7 gene revealed that Irish G6 strains fell within Lineage IV, similiar to previous reports in Ireland.

Conclusion

The detection of unusual G and P combinations may have an impact on rotavirus control programmes and current vaccines may need to incorporate new strains, as the current vaccine available may not offer protection against all of these circulating types.  相似文献   

Detection of numerous verotoxigenic E. coli serotypes, with multiple antibiotic resistance from cattle faeces and soil     

Scott L  McGee P  Walsh C  Fanning S  Sweeney T  Blanco J  Karczmarczyk M  Earley B  Leonard N  Sheridan JJ 《Veterinary microbiology》2009,134(3-4):288-293

Verotoxigenic E. coli (VTEC) belong to a diverse range of serotypes. Serotypes O157 and O26 are predominately identified in VTEC-associated disease in Europe, however due to difficulty in detection little is known about the epidemiology of non-O157 serotypes. This study reports the identification of 7 VTEC serotypes from cattle faeces and soil. Cattle faeces samples (n=128) were taken from animals in 6 different farms, with soil samples (n=20) obtained from 1 farm. After sample incubation in modified tryptone soy broth (mTSB) supplemented with streptomycin sulphate samples were plated onto sorbitol MacConkey (SMAC) also supplemented with streptomycin sulphate. Bacteria detected on the plates were subjected to biochemical testing, antibiotic resistance profiling, and PCR to detect typical virulence genes, beta-lactamase and class 1 integron associated genes. Serotyping was performed on isolates positive for virulence genes. E. coli was identified from 103 samples, with verotoxin genes present in 7 E. coli isolates. Of these 7 isolates, 5 were resistant to 5 or more antibiotics. The isolate resistant to 9 antimicrobials contained a class 1 integron structure. Serotyping identified 7 separate VTEC, O2:H27, O26:H11, O63:H(-), O148:H8, O149:H1, O174:H21 and ONT:H25. Six of these VTEC have been previously associated with human disease, however with the exception of O26:H11, these serotypes have been rarely reported worldwide. Increased surveillance is required to determine the prevalence of these and other non-O157 VTEC. The presence of multi-antibiotic resistance in these isolates is of concern, and the overall implications for public health must be ascertained.  相似文献   

An Irish perspective on Cryptosporidium. Part 1     

Zintl A  Mulcahy G  de Waal T  de Waele V  Byrne C  Clyne M  Holden N  Fanning S 《Irish veterinary journal》2006,59(8):442-447

Cryptosporidiosis, a protozoal disease which causes significant morbidity in humans, is one of the chief causes of diarrhoea in neonatal ruminants. Although the parasite poses a significant threat to public health and animal health in Ireland, its epidemiology on the island is only poorly understood. Environmental studies have shown the waterborne parasite to be widespread in some untreated waterbodies around Ireland. The island's hydrogeological situation, combined with high stocking rates of livestock and the absence of filtration from regular water treatment, render it vulnerable to large-scale outbreaks. This review discusses the parasite in the Irish context and underlines the need for a reference facility to provide active surveillance on the island.  相似文献