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Reza Moazzami Hasan Mirzahoseini Leila Nematollahi Farzaneh Barkhordari Mozhgan Raigani Fatemeh Hajari Taheri Fereidoun Mahboudi Fatemeh Davami 《Iranian Biomedical Journal》2021,25(4):275
Background:Bispecific antibodies represent an important class of mAbs, with great therapeutic potentials due to their ability to target simultaneously two distinct epitopes. The generation of functional bispecific antibodies with the highest possible yields is particularly critical for the production of these compounds on industrial scales. Anti- CD3 × CD19 bsAb is a bispecific T-cell engager (BiTE) currently used for treating ALL. Herein, we have tried to optimize the expression level of this antibody in mammalian hosts. Methods:WPRE sequence was incorporated at the 3’ end of the expression cassette. This modification resulted in a notable about two-fold increase in the expression of the bsAb in the Expi293 cell line. Results & Conclusion:Follow-up flow cytometry analysis demonstrated the binding properties of the produced antibody at acceptable levels, and in vitro bioactivity assays showed that this product is potent enough for targeting and destroying CD19-positive cells. Our findings show that WPRE enhances the expression of this type of bispecific mAbs in HEK-293 family cell lines. This approach can be used in biopharma industry for the mass production of anti-CD3 × CD19 bispecific antibody. Key Words: Acute lymphoblastic leukemia, Bispecific antibodies, Monoclonal antibody 相似文献
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BACKGROUND: Linear alkylbenzene sulfonate (LABS) is an anionic surfactant widely used all over the world. They will eventually end-up and accumulate in household or industrial sewage. Due to their high foaming capabilities which can cause numerous problems in sewage treatment facilities as well as direct toxic effects on many different organisms in ecosystem; they are generally considered as serious pollutants. Many reports have indicated that common bacteria can readily degrade LABS. METHODS: In this survey, two different bacteria were isolated from Tehran municipal active sludge that showed the ability to degrade LABS rapidly and actively upon using it as their sole source of carbon. Biochemical tests as well as 16S rRNA gene sequencing performed. RESULTS: Results have indicated the two isolates to be Acinetobacter johnsoni and Pseudomonas beteli. After experiments to optimize the pH and temperature for growth of the two bacterial isolates, the extent of LABS, utilization was evaluated by HPLC method. The Pseudomonas beteli and Acinetobacter johnsoni isolates were able to degrade 96.4% and 97.2% of the original LABS levels after 10 days of growth, respectively. Mixed culture of the two isolates did not significantly increase LABS utilization (97.6%). CONCLUSION: Our study showed the ability of two isolated steains to rapidly biodegrade LABS under aerobic conditions. 相似文献
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Efficacy of conventional and extended intra-mammary treatment of persistent sub-clinical mastitis with cefquinome in lactating dairy cows 总被引：1，自引：1，他引：0
Kasravi R Bolourchi M Farzaneh N Seifi HA Barin A Hovareshti P Gharagozlou F 《Tropical animal health and production》2011,43(6):1203-1210
The objectives of the present study were to evaluate the efficacy of intra-mammary-administered cefquinome for the treatment
of sub-clinical mastitis in lactating dairy cows and to determine if extended therapy would enhance treatment efficacy. Seventy-three
Holstein dairy cows from a single farm with 150 infected quarters were enrolled in the study. Infected cows were allocated
randomly to one of three treatment regimens: (1) conventional (standard) regimen: 75 mg of cefquinome administered three times
at 16-h intervals (25 infected cows, 52 intra-mammary infections (IMI)), (2) extended regimen: 75 mg of cefquinome administered
six times at 16-h intervals (26 infected cows, 58 IMI) and (3) negative untreated control group (22 cows, 40 IMI). Most IMI
were caused by coagulase-negative staphylococci, streptococci other than Streptococcus agalactiae and coliforms. The overall bacteriological cure (BC) rates for sub-clinical IMI were 84.61%, 91.37% and 20% for the conventional,
extended and the control groups, respectively, indicating a higher BC rate for the treated groups than the control group (P < 0.001). Significant differences in somatic cell count (SCC) were detected between the treated versus the control group
(P < 0.001). No differences, concerning the BC rate or SCC, were observed between the extended and the conventional groups.
Although fat and protein percentages increased in the treated groups, there were no significant differences in post-treatment
milk production between the groups. Results of this study indicate that cefquinome therapy was effective in reducing SCC and
eliminating sub-clinical IMI in lactating dairy cows, but extended therapy did not enhance treatment efficacy. 相似文献
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Somayeh Bohlouli Arezou Rabzia Ehsan Sadeghi Farzaneh Chobsaz Mozafar Khazaei 《Iranian Biomedical Journal》2016,20(1):12-17
Background:
Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) proliferation and their expression of adiponectin receptors.Methods:
In this experimental study, endometrial biopsies (n=7) were taken. ESCs isolation was done by enzymatic digestion and cell filtrations. ESCs of each biopsy were divided into four groups: 0 (control), 10, 100, and 200 ng/ml adiponectin concentrations in three different times (24, 48, or 72 h). The effect of adiponectin on ESC viability and expression of mRNA Adipo receptor1 (R1) and Adipo receptor2 (R2) was determined by Trypan blue staining and semi-quantitative RT-PCR, respectively. Data were analyzed by one-way ANOVA and unpaired student’s t-test, and P<0.05 was considered statistically significant.Results:
Adiponectin inhibited human endometriotic stromal cell proliferation in time- and dose-dependent manners significantly (P=0.001). Expression of AdipoR1 and AdipoR2 gene receptors was increased in human ESCs significantly (P<0.05).Conclusions:
Adiponectin can suppress endometriosis by inhibiting ESC proliferation and increased AdipoR1 and AdipoR2 expression.Key Words: Adiponectin, Adiponectin receptors, Endometriosis, Stromal cells 相似文献26.
Background: Neuroinflammation, as a major outcome of microglia activation, is an important factor for progression of neurodegenerative disorders including Alzheimer''s disease and Parkinson''s disease. Microglial cells, as the first-line defense in the central nervous system, act as a source of neurotoxic factors such as nitric oxide (NO), a free radical which is involved in neuronal cell death. The aim of this study was to inhibit production of NO in activated microglial cells in order to decrease neurological damages that threat the central nervous system. Methods: An in vitro model of a newborn rat brain cell culture was used to examine the effect of betaine on the release of NO induced by lipopolysaccharide (LPS). Briefly, primary microglial cells were stimulated by LPS and after 2 minutes, they were treated by different concentrations of betaine. The production of NO was assessed by the Griess assay while cell viability was determined by the MTT assay. Results: Our investigations indicated that LPS-induced NO release was attenuated by betaine, suggesting that this compound might inhibit NO release. The effects of betaine on NO production in activated microglial cells after 24 h were "dose-dependent". It means that microglial cells which were treated with higher concentrations of betaine, released lower amounts of NO. Also our observations showed that betaine compound has no toxic effect on microglial cells. Conclusion: Betaine has an inhibitory effect on NO release in activated microglial cells and may be an effective therapeutic component to control neurological disorders.Key Words: Betaine, Lipopolysaccharides (LPS), Nitric oxide (NO), Microglia 相似文献
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Z Akhlaghi JI Mobarakeh M Mokhtari H Behnam AA Rahimi MS Khajeh Hosseini F Samiee 《Iranian Biomedical Journal》2012,16(2):107-112
Background: Initial studies have shown that low-energy ultrasound stimulates living tissue cells to reduce regeneration or speed up their recovery. The purpose of this study was to examine the effects of various ultrasound parameters on the speed of recovery in injured sciatic nerves NMRI.Methods: NMRI mice (n = 200) with injured left paw, caused by crushing their sciatic nerves, were randomly selected. The animals were exposed to ultrasound radiation with various frequencies, intensities, and exposure time. They were allocated into 20 groups (19 treatment and 1 control groups). Sciatic functional index (SFI) test was used to evaluate the difference between the groups with respect to functional efficiency of the sciatic nerve and its recovery. SFI ، (P=0.000).Results: The results of SFI test obtained from the 14th day showed a significant difference among the groups (P<0.05). On the 14th day after treatment, one of the groups (US11) recovered up to 90%..Conclusion: Altered ultrasound exposure parameters had more favorable outcomes compared with our previous work.Key Words: Sciatic nerve, Ultrasonic therapy, Regeneration 相似文献
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Sayed Zia Mohammadi Daryoush Afzali Farzaneh Arabpour 《Communications in Soil Science and Plant Analysis》2017,48(21):2496-2505
In the present study, a method based on dispersive liquid–liquid microextraction (DLLME) followed by graphite furnace atomic absorption spectrometry (GFAAS) determination was proposed for the determination of selenium by using ammonium pyrrolidine dithiocarbamate (APDC) as the chelating reagent. The main factors influencing the DLLME were investigated systematically. Under the optimal conditions, the limit of detection for Se(IV) was 0.02 ng mL?1. The relative standard deviation was 4.1% (CSe(IV) = 0.2 ng mL?1, n = 8) with an enhancement factor of 135.8-fold from only 5 mL of the water sample. The proposed method was successfully applied to the determination of Se(IV) in anodic slime and electrolyte samples. In order to validate the proposed method, a Certified Reference Material (trace elements in water, 1643e, NIST) was analyzed, and the determined value obtained was in good agreement with the certified value. 相似文献
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Soraya Abbasi Habashi Farzaneh Sabouni Ali Moghimi Saeed Ansari Majd 《Iranian Biomedical Journal》2016,20(1):33-40