Composition,abundance and phytoplasma infection in the hawthorn psyllid fauna of northwestern Italy     

Rosemarie Tedeschi  Pavel Lauterer  Lorenzo Brusetti  Federica Tota  Alberto Alma 《European journal of plant pathology / European Foundation for Plant Pathology》2009,123(3):301-310

Hawthorn (Crataegus monogyna) is one of the natural hosts of Cacopsylla melanoneura, the acknowledged vector of ‘Candidatus Phytoplasma mali’, the causal agent of Apple Proliferation disease, a serious and growing problem for apple production in Europe, particularly in northern Italy. Wild plants could be important sources of both insects and phytoplasmas, but their role in the epidemiology of phytoplasma diseases and their insect vectors has never been thoroughly examined. Cacopsylla melanoneura’s primary host is hawthorn, a plant closely related to apple which often grows wild near orchards. Other psyllid species feed on hawthorn, but no data are available on their possible role as phytoplasma vectors. We investigated the hawthorn’s psyllid fauna in northwestern Italy using yellow sticky traps, beat trays, and molecular analyses from 2003–2005, to study the relationship between hawthorn, the phytoplasma and the insect vector. Population dynamics were monitored, and insects and hawthorn samples were analysed by polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and DNA sequencing for the presence of phytoplasmas. Cacopsylla melanoneura was the dominant psyllid species, followed by C. peregrina, C. affinis and C. crataegi. PCR and RFLP analyses revealed the presence of different fruit tree phytoplasmas in hawthorn plants, and in all four psyllid species.  相似文献   

Effectiveness of five insecticides for the control of adults and young stages of Cacopsylla melanoneura (Förster) (Hemiptera: Psyllidae) in a semi‐field trial     

Mario Baldessari  Federica Trona  Gino Angeli  Claudio Ioriatti 《Pest management science》2010,66(3):308-312

  相似文献   

Application areas of synchrotron radiation tomographic microscopy for wood research   总被引：2，自引：2，他引：0  

David Mannes  Federica Marone  Eberhard Lehmann  Marco Stampanoni  Peter Niemz 《Wood Science and Technology》2010,44(1):67-84

Possible applications for synchrotron radiation tomographic microscopy in the field of wood research were tested and evaluated at the TOMCAT beamline (TOmographic Microscopy and Coherent rAdiology experimenTs) at the Swiss Light Source (SLS) at the Paul Scherrer Institute (Villigen, Switzerland). For this study, small cylindrical samples (∅ 1 and 3 mm) were examined with different experimental setups resulting in a nominal voxel size of approximately 1.48 × 1.48 × 1.48 and 3.7 × 3.7 × 3.7 μm³, respectively. Suitability of the TOMCAT microscope for 3D investigations of wood anatomy was tested on several softwood and hardwood species revealing microscopic features (e.g. tyloses, wall thickenings or pits) down to the nominal pixel size. The results suggest that even features in the sub-voxel range can be made visible. Tomographic microscopy was also tested for wood technological applications, i.e. penetration behaviour of a wood preservative and also of three wood adhesives (poly-urethane resins) with different viscosities. Although the experiments with the preservative yielded no clear results, the method seems suitable for examining the penetration of the different adhesives. The adhesive penetrates the wood mainly by the vessels where it can be easily discerned from the wood structure.  相似文献   

EVALUATION OF THE FELINE PANCREAS USING COMPUTED TOMOGRAPHY AND RADIOLABELED LEUKOCYTES     

Laurie L.  Head  DVM  Gregory B.  Daniel  DVM  MS  Karen  Tobias  DVM  MS  Federica  Morandi  DVM  MS  Robert C.  Denovo  DVM  MS  Robert  Donnell  DVM  PHD 《Veterinary radiology & ultrasound》2003,44(4):420-428

This study was designed to test the feasibility and utility of computed tomography and radiolabeled granulocytes in evaluating the feline pancreas in six normal cats. Autologous granulocytes were labeled with 99mTc-hexamethylpropyleneamine oxime (HMPAO) and injected into each cat. Whole body scintigraphic images were acquired at 1, 5, 15, and 30 minutes, and 1, 2, and 4 hours following injection. The following day, each cat was anesthetized and computed tomographic images of the abdomen were acquired both pre- and post-contrast. Following CT, a surgical pancreatic biopsy was collected. Feline granulocytes were successfully labeled with 99mTc-HMPAO with a labeling efficiency of 15-42% (average of 27%). An average of 5.42 x 10(7) cells in a 2 mL volume were injected into each cat. Less than 1 minute was required to acquire 500,000 count images. Granulocytes distributed predominantly to the lung, spleen and liver in order of decreasing activity. Only background activity was identified in the region of the pancreas. The pancreas was easily identified on CT images of the abdomen. The pancreas was hypoattenuating relative to both the spleen and liver. The pancreas enhanced with the administration of contrast medium, peaking immediately, then gradually clearing over the 30-minute test period. Following contrast medium administration the pancreas remained hypoattenuating relative to the spleen. All biopsies confirmed the absence of pancreatic inflammation in the study cats and no adverse effects were recognized as a result of pancreatic biopsy. Both computed tomography and radiolabeled granulocytes appear to hold promise as imaging procedures for the detection of feline pancreatitis. We predict that these described normal parameters may be altered in the face of inflammation, facilitating detection of feline pancreatitis. Data from cases of suspect feline pancreatitis are needed to evaluate these methods for clinical utility.  相似文献   

Quantitative assessment of hepatic function by means of 99mTc-mebrofenin in healthy horses     

Morandi F  Frank N  Avenell J  Daniel GB 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2005,19(5):751-755

99mTc-mebrofenin is used in humans and small animals to assess hepatic function. This study was undertaken to measure hepatic clearance of 99mTc-mebrofenin in healthy horses and to determine whether feed deprivation and increased serum total bilirubin (TBIL) concentration alter 99mTc-mebrofenin clearance. Plasma clearance of 99mTc-mebrofenin was determirned in 7 healthy horses at 0, 48, and 96 hours of feed withholding. Serum TBIL and nonesterified fatty acid (NEFA) concentrations were measured every 24 hours. 99mTc-mebrofenin (4.16 +/- 0.62 mCi, mean +/- SD) was injected into a jugular vein, and blood samples were retrieved from the contralateral jugular vein. A plasma time-activity curve of 99mTc-mebrofenin was generated, from which the area under the curve (AUC) and the T1/2 of the fast-phase (T1/2) and slow-phase (T1/2f) were calculated. Mean +/- SD AUC was 17,700 +/- 4,257, 18,616 +/- 8,078, and 16,168 +/- 6,031 counts per minute (cpm) at 0, 48, and 96 hours, respectively; mean +/- SD T1/2f was 2.80 +/- 0.38 minutes, 3.52 +/- 1.46 minutes, and 3.82 +/- 1.29 minutes at 0, 48, and 96 hours, respectively; median T1/2s was 63.9, 49.2, and 45.8 minutes at 0, 48, and 96 hours, respectively. No difference was detected between the values of AUC, T1/2f, and T1/2s at 0, 48, and 96 hours. There was a significant increase in TBIL with fasting, with a mean +/- SD of 6.3 +/- 1.3 mg/dL at 26 hours. NEFAs increased, reaching a plateau at 48 hours (650 +/- 152 micromol/L). Plasma TBIL concentrations did not correlate with AUC or T1/2s but correlated weakly with T1/2f (r = 0.50). Plasma NEFA concentrations did not correlate with AUC, T1/2s, or T1/2f values. This study suggests that 99mTc-mebrofenin plasma clearance is not affected by feed withholding and that hyperbilirubinemia associated with feed withholding does not affect the hepatic extraction efficiency of this radiopharmaceutical.  相似文献   

Correlation of helical and incremental high-resolution thin-section computed tomographic and histomorphometric quantitative evaluation of an acute inflammatory response of lungs in dogs     

Morandi F  Mattoon JS  Lakritz J  Turk JR  Jaeger JQ  Wisner ER 《American journal of veterinary research》2004,65(8):1114-1123

OBJECTIVE: To compare quantitative densitometric computed tomography (CT), morphometric, and histologic data of normal lungs in dogs with similar parameters obtained after induction of an acute inflammatory response and determine whether CT densitometry correlated with histopathologic changes. ANIMALS: 6 healthy adult dogs. PROCEDURE: After initial CT, 1 mL of 0.1 M hydrochloric acid (HCI) and 3 mL of autologous blood were instilled into the right middle (RM) and caudal segment of the left cranial (LCCd) lung lobes, respectively. Immediately and 24 hours after instillation, CT was repeated. At 24 hours, dogs were euthanatized and lungs were fixed and sampled for morphometric and histologic evaluation. The CT data were compared with lung morphology and morphometry by use of unpaired t tests. Comparison with lungs from control dogs was performed using Spearman rank correlation coefficients. RESULT: Mean Hounsfield units (HU) from control and baseline HU from experimental dogs were identical. Immediately after instillation of HCI or blood, there was increased attenuation in both lobes. Autologous blood initially induced severe changes that almost completely resolved at 24 hours; HCI induced severe changes at 24 hours. Significant increases in percentage of parenchymal airspace and alveolar diameter resulted in decreased surface area-to-volume ratio in lobes receiving HCl. Histologic scores were significantly higher in the RM lobe, compared with controls. CONCLUSIONS AND CLINICAL RELEVANCE: Computed tomography attenuation correlated well with histomorphometry and histologic findings in this model. Lung lesions after autologous blood were transient and of limited severity. Lesions induced by HCl were severe; alterations in morphometric and histologic parameters were reflected in CT attenuation measurements.  相似文献   

USE OF ^99MTCO    TRANS-SPLENIC PORTAL SCINTIGRAPHY FOR DIAGNOSIS OF PORTOSYSTEMIC SHUNTS IN 28 DOGS     

Federica Morandi  Robert C. Cole  Karen M. Tobias  Clifford R. Berry  James Avenell  Gregory B. Daniel 《Veterinary radiology & ultrasound》2005,46(2):153-161

Ultrasound-guided percutaneous trans-splenic portal scintigraphy (TSPS) using 99mTcO4(-) has been used to image the portal venous system in normal dogs. Compared with per-rectal portal scintigraphy, it provides higher count density, consistent nuclear venograms of the splenic and portal vein, and significantly decreased radiation exposures. This paper describes the use of TSPS for the diagnosis of portosystemic shunts in 28 dogs. TSPS was performed injecting 70 +/- 28 MBq of 99mTcO4(-) (mean +/- SD) into the splenic parenchyma with ultrasound guidance. A dynamic acquisition at a frame rate of four frames/s for 5 min was initiated after placement of the needle and approximately 2s prior to injection. All dogs had diagnoses confirmed via exploratory laparotomy or ultrasonographic identification of the shunting vessel(s). Three studies (10.7%) were nondiagnostic because of intraperitoneal rather than intrasplenic injection of the radionuclide. Three pathways were recognized on the scintigraphic images: (1) portoazygos shunts--the 99mTcO4(-) bolus traveled dorsally, running parallel to the spine and entering the heart craniodorsally; (2) single portocaval or splenocaval shunts--the 99mTcO4(-) bolus ran from the area of the portal vein/splenic vein junction in a linear fashion toward the caudal vena cava entering the heart caudally; (3) internal thoracic shunt-the 99mTcO4 bolus traveled ventrally along the thorax and abdomen entering the cranial aspect of the heart. Single and multiple shunts were easily distinguished. There were no distinguishing features between single intra and extrahepatic portocaval shunts.  相似文献   

High throughput detection of bluetongue virus by a new real-time fluorogenic reverse transcription-polymerase chain reaction: application on clinical samples from current Mediterranean outbreaks.     

Miguel Angel Jiménez-Clavero  Montserrat Agüero  Elena San Miguel  Tomás Mayoral  Maria Cruz López  María José Ruano  Esther Romero  Federica Monaco  Andrea Polci  Giovanni Savini  Concepción Gómez-Tejedor 《Journal of veterinary diagnostic investigation》2006,18(1):7-17

  相似文献   

Expression of c-kit proto-oncogene in canine mastocytoma: a kinetic study using real-time polymerase chain reaction.     

Lauretta Turin  Fabio Acocella  Damiano Stefanello  Angelo Oseliero  Danilo Fondrini  Stefano Brizzola  Federica Riva 《Journal of veterinary diagnostic investigation》2006,18(4):343-349

  相似文献   

PDGFs and PDGFRs in canine osteosarcoma: New targets for innovative therapeutic strategies in comparative oncology     

Lorella Maniscalco  Selina Iussich  Emanuela Morello  Marina Martano  Bartolomeo Biolatti  Fulvio Riondato  Leonardo Della Salda  Mariarita Romanucci  Daniela Malatesta  Laura Bongiovanni  Federica Tirrito  Francesca Gattino  Paolo Buracco  Raffaella De Maria 《Veterinary journal (London, England : 1997)》2013,195(1):41-47

Platelet derived growth factor receptor (PDGFR)α and PDGFRβ are tyrosine kinase receptors that are overexpressed in 70–80% of human osteosarcomas (OSAs) and may be suitable therapeutic targets for specific kinase inhibitors (TKIs). Canine OSA shows histopathological and clinical features similar to human OSA, and is considered an excellent model in comparative oncology. This study investigated PDGF-A, PDGF-B, PDGFRα and PDGFRβ expression in 33 canine OSA samples by immunohistochemistry and in seven primary canine OSA cell lines by Western blot and quantitative PCR analysis.Immunohistochemical data showed that PDGF-A and PDGF-B are expressed in 42% and 60% of the OSAs analysed, respectively, while PDGFRα and PDGFRβ were expressed in 78% and 81% of cases, respectively. Quantitative PCR data showed that all canine OSA cell lines overexpressed PDGFRα, while 6/7 overexpressed PDGFRβ and PDGF-A relative to a normal osteoblastic cell line. Moreover, in vitro treatment with a specific PDGFR inhibitor, AG1296, caused a dose- and time-dependent decrease in AKT phosphorylation. Collectively, these data show that PDGFRs/PDGFs are co-expressed in canine osteosarcomas, which suggests that an autocrine and/or paracrine loop is involved and that they play an important role in the aetiology of OSA. PDGFRs may be suitable targets for the treatment of canine OSA with a specific TKI.  相似文献