Deleterious Effects of Progestagen Treatment in VEGF Expression in Corpora Lutea of Pregnant Ewes     

CA Letelier  MA Sanchez  RA Garcia‐Fernandez  B Sanchez  P Garcia‐Palencia  A Gonzalez‐Bulnes  JM Flores 《Reproduction in domestic animals》2011,46(3):481-488

The aim of the current study was to determine the possible effects of progestagen oestrous synchronization on vascular endothelial growth factor (VEGF) expression during sheep luteogenesis and the peri‐implantation period and the relationship with luteal function. At days 9, 11, 13, 15, 17 and 21 of pregnancy, the ovaries from 30 progestagen treated and 30 ewes cycling after cloprostenol injection were evaluated by ultrasonography and, thereafter, collected and processed for immunohistochemical evaluation of VEGF; blood samples were drawn for evaluating plasma progesterone. The progestagen‐treated group showed smaller corpora lutea than cloprostenol‐treated and lower progesterone secretion. The expression of VEGF in the luteal cells increased with time in the cloprostenol group, but not in the progestagen‐treated group, which even showed a decrease between days 11 and 13. In progestagen‐treated sheep, VEGF expression in granulosa‐derived parenchymal lobule capillaries was correlated with the size of the luteal tissue, larger corpora lutea had higher expression, and tended to have a higher progesterone secretion. In conclusion, the current study indicates the existence of deleterious effects from exogenous progestagen treatments on progesterone secretion from induced corpora lutea, which correlate with alterations in the expression of VEGF in the luteal tissue and, this, presumably in the processes of neoangiogenesis and luteogenesis.  相似文献   

Single Layer Centrifugation of Stallion Spermatozoa through Androcoll™‐E does not Adversely Affect their Capacitation‐Like Status,as Measured by CTC Staining     

A‐S Bergqvist  A Johannisson  L Bäckgren  A‐M Dalin  H Rodriguez‐Martinez  JM Morrell 《Reproduction in domestic animals》2011,46(1):e74-e78

This study was designed to evaluate the effect of single layer centrifugation (SLC) and subsequent cold storage on stallion sperm capacitation‐like status and acrosome reaction. Three stallions were included in the study, with three ejaculates per stallion. The samples were examined 4, 24 and 72 h after collection, extension and SLC, with storage at 6°C. Sperm capacitation‐like status was investigated using the fluorescent dye chlortetracycline (CTC). There was no difference in capacitation‐like status between colloid‐selected and non‐selected spermatozoa. Sperm motility decreased significantly during cold storage, whereas the proportion of apparently capacitated spermatozoa increased. There was no change in the proportion of acrosome‐reacted spermatozoa. In conclusion, SLC through Androcoll?‐E does not adversely affect the capacitation‐like status of stallion spermatozoa, although it did increase with time during cold storage.  相似文献   

Genetic parameters of product quality and hepatic metabolism in fattened mule ducks     

Marie-Etancelin C  Basso B  Davail S  Gontier K  Fernandez X  Vitezica ZG  Bastianelli D  Baéza E  Bernadet MD  Guy G  Brun JM  Legarra A 《Journal of animal science》2011,89(3):669-679

Genetic parameters of traits related to hepatic lipid metabolism, carcass composition, and product quality of overfed mule ducks were estimated on both parental lines of this hybrid: the common duck line for the maternal side and the Muscovy line for the paternal side. The originality of the statistical model was to include simultaneously the additive genetic effect of the common ducks and that of the Muscovy ducks, revealing a greater genetic determinism in common than in Muscovy. Plasma metabolic indicators (glucose, triglyceride, and cholesterol contents) were heritable, in particular at the end of the overfeeding period, and heritabilities increased with the overfeeding stage. Carcass composition traits were highly heritable in the common line, with values ranging from 0.15 for liver weight, 0.21 for carcass weight, and 0.25 for abdominal fat weight to 0.32 for breast muscle weight. Heritabilities of technological outputs were greater for the fatty liver (0.19 and 0.08, respectively, on common and Muscovy sides for liver melting rate) than for the pectoralis major muscle (between 0.02 and 0.05 on both parental sides for cooking losses). Fortunately, the processing industry is mainly facing problems in liver quality, such as too high of a melting rate, than in meat quality. The meat quality appraisal criteria (such as texture and cooking losses), usually dependent on pH and the rate of decline of pH, were also very lowly heritable. This study demonstrated that genetic determinism of meat quality and ability of overfeeding is not similar in the common population and in the Muscovy population; traits related to fattening, muscle development, and BW have heritability values from 2 to 4 times greater on the common line than on the Muscovy line, which is relevant for considering different selection strategies.  相似文献   

Are one early muscle pH and one early temperature measurement sufficient to detect PSE breast meat in turkeys?     

Eadmusik S  Molette C  Fernandez X  Rémignon H 《British poultry science》2011,52(2):177-188

1. Within a large flock of turkey toms (2000 BUT9 conventionally reared and slaughtered), early muscle pH measurements were randomly done to distinguish two groups of birds presenting low (fast glycolysing, GR) or normal (normal glycolysing, GN) values. 2. Subsequently, ultimate pH values and meat quality parameters were also recorded. Meat quality parameters from GR or GN samples differ more or less indicating more or less severe PSE conditions. Proteins extracted from the samples at 20 min post mortem were similar while they differed greatly at 24 h post mortem. Moreover, among the GR birds, a subgroup of animals (called AB) presented SDS-PAGE profiles largely different from other GR or GN birds. 3. All the subsequent analysis developed on meat quality parameters as well as for protein extractabilities also differed between AB and other animals indicating that they must be considered differently in term of PSE syndrome development. 4. Western blots against Myosin Heavy Chain and actin at 24 h post mortem indicate that myofibrillar protein alterations are different in AB and GR or GN samples. 5. At 20 min post mortem, glycogen content was lowest in AB samples while the glycolytic potential was similar in all samples at the time of death. Measurements of PFK enzyme specific activity did not indicate a different regulation of post mortem glycolysis in AB samples. 6. Our results suggest that a unique pH measurement at 20 min post is insufficient to detect animals more prone to developing a severe PSE syndrome in turkeys. In consequence, it is suggested that a more precise evaluation of the kinetics of pH and temperature decrease has to be conducted to understand the aetiology of meat quality parameter alterations in poultry.  相似文献   

Polycyclic Aromatic Hydrocarbons in Soils from European High Mountain Areas   总被引：1，自引：0，他引：1  

Roberto Quiroz  Joan O. Grimalt  Pilar Fernandez  Lluis Camarero  Jordi Catalan  Evzen Stuchlik  Hansjoerg Thies  Ulrike Nickus 《Water, air, and soil pollution》2011,215(1-4):655-666

Polycyclic aromatic hydrocarbons (PAHs) were analyzed in 70 soils distributed in mountain areas such as Montseny (300?C1,700 m), Pyrenees (1,500?C2,900 m), Alps (1,100?C2,500 m), and Tatras (1,400?C1,960 m). Average total PAH concentrations, excluding retene and perylene, were about 400 ng/g in the Pyrenees and 1,300?C1,600 ng/g in the other mountain ranges. No correlations between PAH concentrations and total organic carbon were observed. Retene was the major PAH in the Pyrenean soils of lower altitude. No altitudinal dependence was found between soil PAH concentrations and elevation for the whole dataset. However, in the Tatra soils a statistically significant correlation with altitude was observed involving higher concentrations at higher altitude. This correlation was due to the statistically significant altitudinal dependence of the more volatile PAHs. Another observed altitudinal trend concerned the benz[a]anthracene/(benz[a]anthracene + chrysene + triphenylene) and the benzo[a]pyrene/(benzo[a]pyrene + benzo[e]pyrene) ratios that exhibited a decrease in the more chemically labile compounds, benz[a]anthracene and benzo[a]pyrene, respectively, in the soils located at higher altitude. This observation is consistent with the expected higher photooxidation at higher mountain altitude.  相似文献   

Invasive histiocytoma in the ear canal of a dog     

Monica Fernandez  Bonnie B. Boudreaux  Leslie Wilson  Peter F. Moore  Nathalie Rademacher  Cherie Pucheu‐Haston 《Veterinary dermatology》2020,31(4):317-e80

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Chlamydia psittaci: a suspected cause of reproductive loss in three Victorian horses     

R Akter  AW Stent  FM Sansom  JR Gilkerson  C Burden  JM Devlin  AR Legione  CM El-Hage 《Australian veterinary journal》2020,98(11):570-573

Chlamydia psittaci was detected by PCR in the lung and equine foetal membranes of two aborted equine foetuses and one weak foal from two different studs in Victoria, Australia. The abortions occurred in September 2019 in two mares sharing a paddock northeast of Melbourne. The weak foal was born in October 2019 in a similar geographical region and died soon after birth despite receiving veterinary care. The detection of C. psittaci DNA in the lung and equine foetal membranes of the aborted or weak foals and the absence of any other factors that are commonly associated with abortion or neonatal death suggest that this pathogen may be the cause of the reproductive loss. The detection of C. psittaci in these cases is consistent with the recent detection of C. psittaci in association with equine abortion in New South Wales. These cases in Victoria show that C. psittaci, and the zoonotic risk it poses, should be considered in association with equine reproductive loss in other areas of Australia.  相似文献   

Temporal changes in clinical and radiographic variables in dogs with preclinical myxomatous mitral valve disease: The EPIC study     

Adrian Boswood  Sonya G. Gordon  Jens Häggström  Martin Vanselow  Gerhard Wess  Rebecca L. Stepien  Mark A. Oyama  Bruce W. Keene  John Bonagura  Kristin A. MacDonald  Mark Patteson  Sarah Smith  Philip R. Fox  Karen Sanderson  Richard Woolley  Viktor Szatmári  Pierre Menaut  Whitney M. Church  M. Lynne O'Sullivan  Jean-Philippe Jaudon  Jan-Gerd Kresken  John Rush  Kirstie A. Barrett  Steven L. Rosenthal  Ashley B. Saunders  Ingrid Ljungvall  Michael Deinert  Eric Bomassi  Amara H. Estrada  Maria J. Fernandez Del Palacio  N. Sydney Moise  Jonathan A. Abbott  Yoko Fujii  Alan Spier  Michael W. Luethy  Roberto A. Santilli  Masami Uechi  Anna Tidholm  Christoph Schummer  Philip Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(3):1108-1118

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Mineral composition of iron chlorotic citrus limon L. leaves     

J. A. Fernandez‐Lopez  J. M. Lopez‐Roca  L. Almela 《Journal of plant nutrition》2013,36(8):1395-1407

A field experiment was conducted to evaluate the effect of iron (Fe) chlorosis on the mineral composition of lemon tree leaves cultivated in calcareous soil in the region of Murcia, Spain. A Fe‐polyflavonoid was employed as fertilizer to control Fe deficiency. The evolution of the leaf content in Fe, potassium (K), sodium (Na), magnesium (Mg), calcium (Ca), phosphorus (P), manganese (Mn), and zinc (Zn) was monitored during all the sampling cycle. A high correspondence between total Fe and chlorophyll content could be appreciated. A multivariance analysis was performed with the data obtained, showing that Fe was correlated with P, Mn, and K.  相似文献   

Fatty acid,amino acid,and trace mineral analyses of five weaning foods from Jos,Nigeria     

Fernandez DR  Vanderjagt DJ  Williams M  Huang YS  Chuang LT  Millson M  Andrews R  Pastuszyn A  Glew RH 《Plant foods for human nutrition (Dordrecht, Netherlands)》2002,57(3-4):257-274

Five plant-based weaning foods (WF) (Dietrend, Jot-M, Soy, Ang and Vic-T) locallyprepared in Jos, Nigeria were analyzed by gas-liquid chromatography,reverse-phase high performance liquid chromatography, and atomicemission spectrometry with inductively coupled plasma to determine theirfatty acid (FA), amino acid, and trace mineral contents, respectively.Results of these direct analyses were compared to expected values derivedfrom food composition tables prepared by the United States Department of Agriculture (USDA). Additionally, results were compared against recommendednutrient values, using breast milk as the standard for FA content andrecommended dietary allowances (RDA) for amino acid and mineral contents.The overall nutritional value of the five WF varied considerably and thequantities of particular nutrients determined by direct analysis differedmarkedly from those estimated using USDA food tables. Comparison of WFfatty acid composition relative to the RDA recommendations and a humanmilk standard revealed a much higher proportion of both linoleic (35–55wt%) and -linolenic acids (1%–7 wt%) relative to human milklipids (11%–12% and 0.8%–0.9% wt, respectively); however, the WFwere devoid of arachidonic acid and docosahexaenoic acid. Soy containedthe highest amounts of linoleic acid (59.7 mg/g) and -linolenicacid (7.46 mg/g) compared to the other four WF (10.2–41.0 and 0.35–3.18 mg/g, respectively). The linoleic acid/-linolenic acid ratio was within the recommended range (5:1 to 10:1) in only Jot-M (10:1)and Soy (8:1). Dietrend, Vic-T and Ang, containedlinoleic/-linolenic ratios of 12:1, 29:1, and 82:1, respectively.The Soy weaning food would provide the most protein (24.3 g/day), basedon an estimated daily intake of 65 g of weaning food by a normalsix-month-old infant, compared to Jot-M (11.9 g/day), Dietrend (11.7g/day), Ang (8.07 g/day) and Vic-T (7.26 g/day). The protein RDA forchildren up to 1 year of age is 13–14 g/day. Comparison of the mineralcontents of the WF to the RDAs for various minerals indicated that all fivewould provide suboptimal amounts of calcium (16 to 250 mg/day) andzinc (1.42 to 3.56 mg/day) compared to respective RDAs of400 mg/day and 5 mg/day.These data show that the Soy weaning food is an excellent source of linoleicacid and -linolenic acid, as well as being a good source of highquality protein. Jot-M and Dietrend provide useful amounts of the essentialFA; however, it is advisable to reevaluate the composition of Ang andVic-T to find ways to improve the linoleic/-linolenic ratio of eachand increase their total protein content. These results document theshortcomings of using published food composition tables based on foods inAmerica when devising weaning foods based on ingredients in another partof the world.  相似文献