Erträge und n‐bilanzen im IOSDV Madrid im laufe von vier rotationen     

Cristina López‐Fando  Maria Teresa Pardo Fernandez  Hans‐Richard Wegener 《Archives of Agronomy and Soil Science》2013,59(6):489-505

Im IOSDV Madrid wird seit 1984 die kombinierte Wirkung von Stallmist, Stroh, Zwischenfrucht und mineralischer N‐Düngung in der Fruchtfolge Sorghum‐Weizen‐Gerste auf einem semiariden Standort in der zentralspanischen Meseta auf einem Calcic Luvisol geprüft.

Die Ergebnisse zeigen, dass die Witterungsbedingungen die Erträge wesentlich stärker beeinflussen als die Behandlungsvarianten. Durch die sehr geringen Niederschläge während der dritten Rotation waren die Gersten‐ und Weizenerträge unabhängig von der Düngung sehr niedrig, während unter optimalen Niederschlagsbedingungen in der ersten und zweiten Rotation die Weizen‐ und Gerstenerträge mit steigender N‐Düngung zunahmen und ihr Optimum bei der Stickstoffdüngungsstufe N₃ aufwiesen; allerdings zeigte nur die Gerste eine Reaktion auf die gleichzeitige Anwendung organischer Dünger. Während der vierten Rotation wurden die Trockenmasse‐Erträge bei Weizen und Gerste durch organische Düngung bis zur Stufe N₂ erhöht, während höhere Mineral‐N‐Gaben die Erträge negativ beeinflussten. Bei Sorghum traten, bedingt durch die extremen Niederschlagsbedingungen, erhebliche Schwierigkeiten auf, die sich in einem sehr niedrigen Korn‐Stroh‐Verhältnis widerspiegelten und keinerlei signifikanten Zusammenhang zu den Behandlungsvarianten erkennen liessen.  相似文献   

What is your diagnosis? Cerebrospinal fluid from a dog     

Nicole Fernandez  Jennifer L Davies  Jose Diaz  Tim Spotswood 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2013,42(2):244-245

  相似文献   

Unique presentation of normolipaemic cutaneous xanthoma in a cat     

PA Ravens  LJ Vogelnest  SA Piripi 《Australian veterinary journal》2013,91(11):460-463

  相似文献   

In vitro susceptibilities of feline and canine Escherichia coli and Pseudomonas spp. isolates to ticarcillin and ticarcillin–clavulanic acid     

AB Bennett  PA Martin  SA Gottlieb  M Govendir 《Australian veterinary journal》2013,91(5):171-178

  相似文献   

Bovine colostrum-induced anaemia in a 2-week-old lamb     

RE Ruby  CC Balcomb  SA Hunter  KE Lawrence 《New Zealand veterinary journal》2013,61(1):82-83

  相似文献   

Urethane anesthesia in adult female rats: preliminary observations     

FJ  Golder  SA Robertson  A Valverde  DC Bolser 《Veterinary anaesthesia and analgesia》2003,30(2):115-115

Urethane is widely used as a rodent anesthetic in the laboratory setting, and is characterized as producing long‐lasting anesthesia. The purpose of this study was to evaluate the quality of anesthesia provided by a single dose of urethane based on the response to a noxious stimulus. If the quality of anesthesia was insufficient to prevent gross purposeful movement (GPM), isoflurane was also administered until no response to noxious stimulation occurred. Five adult Harlan Sprague Dawley rats (6 months of age, 250–300 g) were given urethane (1.4 g kg^?1 IP) and evaluated for 120 minutes post‐injection. If the rats became laterally recumbent by 20 minutes post‐injection, a large hemostat was positioned around the tail and the response to tail clamping was assessed. If no GPM occurred, an additional 20 minutes was allowed to elapse. If the rats were not laterally recumbent or GPM was present, they were placed in a chamber and isoflurane in oxygen was administered. Inspired isoflurane concentrations (ISO) were measured using a S/5 anesthetic gas analyzer (Datex‐Ohmeda Division, Helsinki, Finland) calibrated before each experiment with a standardized calibration gas mixture (DOT‐34 NRC 300/375 m 1014, Datex‐Ohmeda Division, Helsinki, Finland). A period of 20 minutes was allowed for equilibration to inspired ISO. The tail‐clamp stimulus was then re‐applied and the animal's response recorded. If GPM was absent, ISO was lowered by 10–20% and an additional 20 minute interval elapsed. In contrast, if GPM was present, ISO was increased by 10–20%. This procedure was repeated until the ISO required to prevent GPM was determined in duplicate. The position within the estrus cycle influenced pain thresholds in the rats. As such, a vaginal smear was prepared from each rat and the position in the estrus cycle was determined based on vaginal cytology. Rats were euthanatized at the end of the study period. All values were mean ± SD. Four rats became recumbent after urethane injection (time to recumbency: 45 ± 17 seconds). Of these, two rats (one estrus, one metestrus) did not require isoflurane supplementation for the duration of the study. The three remaining rats (two metestrus, one estrus) required isoflurane supplementation. The mean ISO required to prevent GPM was 0.26 ± 0.16%. Position within the estrus cycle did not appear to affect the animal's response to urethane. These results indicate that urethane anesthesia is not long lasting in all rats and provides variable quality of anesthesia. This is of particular concern in the laboratory setting where muscle relaxants are often administered to rats shortly after urethane injection.  相似文献   

蛋白质组学研究进展及其在解决家禽肉质问题中的应用     

H. Remignon  C. Molette  R. Babile  X. Fernandez  束婧婷  包文斌 《国外畜牧学(猪与禽)》2006,(3):43-46

蛋白质是几乎所有生物学过程的功能单位,而蛋白质组是指由一个细胞在特定时间和特定环境条件下所有蛋白质的表达。由于肌肉主要由水和蛋白质组成,因而对蛋白质组进行分析,就能提供海量的信息,向我们展示参与决定肉质的各种生理机制过程中的蛋白质的结构和功能。本文的目的是介绍最近应用于蛋白质组分析的最经典的技术,如样品的准备、蛋白增溶、2-DE法或SDS-PAGE法蛋白质分离、蛋白检测和定量、电脑分析2-DE结果以及最终鉴定。与肉质相关的蛋白质组研究,尤其是家禽肉质的研究,还很少;但一些研究结果显示出蛋白质组方法可以从全新的角度来分析新出现的或已知的肉质问题。蛋白质组分析不仅已经给予了哺乳动物肉的嫩度研究以新的亮点,也为解释火鸡PSE肉综合征提出了新的假设。一些研究还报告了有关鸡肌肉生长发育的有意义的结果。肉质是一个复杂的综合问题,遗传、饲养管理、运输、屠宰以及肉品加工过程中对肉的处理方法等诸多因素都会影响肉质。利用蛋白组学方法可以从不同的角度重新认识这些问题,并且有助于生物学家们理解并最终解决这些问题。  相似文献   

基于高温诱发树木多倍体的模糊PID温度控制器     

章玮玮  撒潮  李慧  李丽 《林业机械与木工设备》2006,34(4):24-27

针对树木枝芽的室外高温诱发多倍体处理,介绍了一种模糊PID温度控制器,该控制器具有显示温度、设定温度、自动控制温度等功能。控制器采用PID参数模糊自整定方法,充分发挥了其动态响应好、上升时间快、超调小的特点,具有较高的动态跟踪品质和稳态精度,并在实际应用中取得了较好的控制效果。  相似文献   

Fruit Quality and Composition of Two Advanced North Carolina Strawberry Selections     

Penelope Perkins-Veazie  Jeremy Pattison  Gina Fernandez  Guoying Ma 《International Journal of Fruit Science》2016,16(4):220-227

ABSTRACT

The advanced selections, NCS 10-038 and NCS 10-156, from the North Carolina breeding program were compared to the traditionally used cultivars, Camarosa and Chandler, for storage life and fruit composition in 2014 and 2015. Postharvest quality of NCS 10-038 was similar to that of ‘Camarosa’ and ‘Chandler’ after 8 days storage at 4 °C. NCS 10-156 was significantly worse in overall appearance and had more fruit shrivel, calyx browning, and mold than the other genotypes. However, NCS 10-156 was highest in soluble solids content and was similar in total anthocyanin content and total phenolic content to ‘Camarosa’ and ‘Chandler’. Further analysis of flavonoids by high performance liquid chromatography showed that NCS 10-156 was much lower in total flavonols than the other genotypes and comparable in anthocyanin pigments to ‘Chandler’. NCS 10-038, while similar in pigment profile to ‘Camarosa’, had less total anthocyanin than the other genotypes. NCS 10-038 had a lighter red color than the other genotypes and was similar in postharvest quality to ‘Chandler’ and ‘Camarosa’ and may be suitable for long-distance markets. NCS 10-156 is slightly softer than the other genotypes and more prone to mold while the higher soluble solids content may make it suitable for direct market sales. Both selections show postharvest promise for strawberry production in the humid mid-South region.  相似文献   

Demonstration of non-inferiority of a novel combination intramammary antimicrobial in the treatment of clinical mastitis     

MA Bryan  SY Hea  SA Mannering  R Booker 《New Zealand veterinary journal》2016,64(6):337-342

AIM: To test the non-inferiority of a novel combination intramammary product containing penicillin and cloxacillin to a reference intramammary product containing oxytetracycline, oleandomycin, neomycin and prednisolone with regard to bacteriological cure and clinical cure.

METHODS: Clinical cases of mastitis were sourced from 30 spring-calving dairy farms in the Southland region of New Zealand. Affected quarters were infused three times at 24 hourly intervals with either the novel combination product containing 1?g penicillin and 200?mg cloxacillin, or a reference product containing 200?mg oxytetracycline, 100?mg oleandomycin, 100?mg neomycin and 5?mg prednisolone. Cows were enrolled when a farmer detected a case of clinical mastitis. Milk samples were collected for microbiological culture immediately before treatment (Day 0) and on Days 9, 16 and 23. Bacteriological cure was compared for 187 and 178 quarters treated with the reference and novel product, respectively, and clinical cure was compared for 235 and 223 quarters, respectively. Non-inferiority was assessed by calculating the difference in cure rates between the two products and constructing a 95% CI around the difference, using the variance inflation factor to account for herd level clustering. The non-inferiority margin was 20% for both bacteriological and clinical cure. Generalising estimating equation models were used to determine predictor variables.

RESULTS: The bacteriological cure percentage, adjusted to account for herd-level clustering, was 8.5 (95% CI=?1.7–21.8)% higher for quarters treated with the novel than the reference product. The adjusted clinical cure percentage was 0.3 (95% CI=?11.2–12.0)% higher for clinical quarters treated with the novel than the reference product. Bacterial species was the only covariate for bacteriological cure (p=0.003), and quarter score at enrolment (indicating udder inflammation) was the only covariate for clinical cure (p=0.032) in the multivariable models.

CONCLUSION: The novel combination product was demonstrated to be non-inferior to the reference product with regards to both bacteriological cure and clinical cure.

CLINICAL RELEVANCE: Clinicians treating mastitis now have access to this novel combination intramammary product, and demonstration of its non-inferiority compared to the existing reference product will provide options for treatment approaches. The novel product contains fewer antimicrobials; which are of a narrower spectrum of activity.  相似文献