Probiotic characteristics and aflatoxin B1 binding ability of Debaryomyces hansenii and Kazaschtania exigua from rainbow trout environment          下载免费PDF全文

María P Martínez  María L González Pereyra  María G Fernandez Juri  Valeria Poloni  Lilia Cavaglieri 《Aquaculture Research》2018,49(4):1588-1597

The aim of this study was to evaluate probiotic properties and the aflatoxin B₁ adsorption ability of yeasts isolated from rainbow trout intestine and fish feed to assess their use in the formulation of feed additives. Growth at pH 2, bacterial pathogens inhibition, bacterial pathogens co‐aggregation, autoaggregation, homologous and heterologous inhibition against lactic acid bacteria were evaluated. Moreover, aflatoxin B₁ adsorption was tested. All strains were able to maintain viable (10⁷ cells/ml) at pH 2. All strains isolated from intestine were identified as Kazaschtania exigua, while strains isolated from feed were all identified as Debaryomyces hansenii. Kazaschtania exigua RC035 and RC037 showed the strongest antimicrobial activity while K. exigua RC037 and RC038 were the most efficient co‐aggregating bacterial pathogens. All strains exhibited strong autoaggregation. None of the tested yeast strains showed homologous inhibition towards other yeasts and heterologous inhibition towards lactic acid bacteria strains. Debaryomyces hansenii RC031 demonstrated aflatoxin B₁ adsorption capacity (21%). The results of the present study indicate that select strains of Kazaschtania exigua and D. hansenii showed potential to improve the health of rainbow trout by inhibiting pathogens and binding AFB₁ and their use as probiotics may improve the production of rainbow trout in aquaculture systems.  相似文献   

豆米轮作对春玉米土壤固氮细菌群落结构及多样性的影响     

提俊阳  张玉芹  杨恒山  张瑞富  邰继承  萨如拉  韩镁琪 《中国农学通报》2023,39(2):44-50

为探明大豆-玉米轮作对土壤固氮细菌的影响，以玉米连作为对照(MMM)，以nif-H基因为指示，采用高通量测序技术，分析大豆-玉米2年轮作周期(MSM)和大豆-玉米-玉米3年轮作周期(SMM)下土壤固氮细菌群落结构及多样性。结果表明：大豆-玉米轮作下0～20、20～40 cm土层土壤有机质、全氮、碱解氮、铵态氮和硝态氮均显著增加。固氮细菌的Chao1指数和ACE指数在0～20、20～40 cm土层均为轮作高于对照，轮作下MSM显著高于SMM;Shannon指数和Simpson指数在0～20 cm土层轮作显著高于MMM;20～40 cm土层MSM显著高于MMM。在门水平上，MSM和SMM的0～20、20～40 cm土层变形菌门的相对丰度均高于MMM。在属水平上，2个轮作类型0～20 cm土层的慢生根瘤菌属、弗兰克氏菌属和纤毛菌属相对丰度显著较高；20～40 cm土层慢生根瘤菌属、伪食酸菌属、地杆菌属和固氮弧菌属相对丰度显著较高；MSM 0～20、20～40 cm土层慢生根瘤菌属、甲基孢囊菌属和固氮弧菌属较SMM均提高。方差分析表明，固氮细菌丰度指数（Chao1和ACE指数）与有机质、碱解氮...  相似文献