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71.
In the common bean crop in Brazil, the requirement of the value for cultivation and use trials is that these experiments must be conducted over two years in three locations per region. Information in regard to the necessary number of years to ensure precision in cultivar recommendation and the influence of evaluated years in the estimation of the GE interaction are still scarce. Using grain yield of five check varieties assessed over 11 years in three sowing seasons per year, the aims of this study are as follows: to measure the relative contribution of the GE interaction, evaluating the implication of the number of years in the estimates of the GE interaction, and infer how many years are needed to ensure precision in cultivar recommendation. For instances, analysis of variance was carried out involving all environments and also combinations of years. The results showed that the GE interaction was greater than all other cross‐effects involving lines. The use of at least two years allows good coincidence in cultivar recommendation compared to the whole period. Increasing the evaluation time is a good strategy, especially when it is difficult to grow three different sowing seasons.  相似文献   
72.
Sclerotinia sclerotiorum infections on sunflower capitula produce white rot, one of the most dangerous diseases sunflower bears in all humid areas. Therefore, disease quantification on developed genetic materials is a very important action in sunflower breeding. Given the horizontal type of resistance these evaluations should be made in different environments. Several hybrids obtained after crossing seven female × seven male sunflower inbred lines in a factorial mating design were evaluated for resistance to white rot in two locations in the southeast of the province of Buenos Aires, Argentina. Disease incidence and the symptom appearance index indicated significant genotypic effects and genotype × environment (G × E) interactions. Genotypic effects were a greater source of variance than the G × E interaction effects. The G × E interactions only represented changes in magnitude. These results were used to identify the best genotypes for both resistance factors. Four hybrids showed low disease incidence values under both artificial and natural infections, of which only two had high values of the symptom appearance index at both locations. These hybrids are considered to have adequate resistance attributes for the ascosporic penetration and the mycelium extension in the capitulum tissue and could therefore be sown in the province of Buenos Aires without increasing risks of S. sclerotiorum attacks.  相似文献   
73.
Red clover (Trifolium pratense L.) is one of the main forage species of temperate regions. Cultivars of red clover are heterogeneous which makes their genetic analysis difficult. We applied RAPDs (Random Amplifed Polymorphic DNA) in order to assess the genetic relationship and levels of genetic variability existing among a group of 16 elite red clover parents organised in four subsets of 4 parents each. Out of 55 primers 21 provided reproducible results. A total of 135 reliable and polymorphic RAPD bands were detected which were used to estimate genetic distances among pair-wise combinations of elite parents. Nei and Li's similarity values ranged from 0.60 to 0.77, with a mean of 0.66, which reflects a rather high genetic variability among the genotypes evaluated. Lower levels of genetic variability, as detected by polymorphic loci and mean heterogeneity values, were detected in a subset of parents selected for resistance to the stem nematode. Cluster analyses resolved the different sets of parents in a manner consistent with what is known from their breeding origins. An Analysis of Molecular Variance detected substantial levels of variation within subsets of parents. RAPDs represent a valuable source of genetic information for red clover breeding programmes. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   
74.
The objective was to evaluate the effects of omitting macronutrients in the nutrients solution on growth characteristics and nutritional status of eggplants. The treatments were complete nutrients solution and solutions with nutrient omission: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S). The experiment was carried out under greenhouse conditions with three replicates in a completely random design. Plant height, number of leaves per plant, leaf area, relative chlorophyll index, photosynthesis rate, stomatal conductance, dry matter, concentration levels of macronutrients in plant aerial part and root system, and nutritional disorders were evaluated. Omitting elements interfered in the concentration of elements in the various plant tissues and this had as consequences limited vegetative growth, reduced dry matter and led to the development of the typical deficiency symptoms of each element. Although potassium was the most demanded of all elements, nitrogen and calcium were the most growth limiting ones.  相似文献   
75.
All the accessions (35) of white lupin (Lupinus albus L.) landraces collected from northwest Iberian plateau and maintained at the Spanish germplasm collection (CRF of INIA), were studied with the general objective of assessing the biodiversity of these landraces and to ascertain their value as genetic resources for the development of germplasm adapted to the areas where they were collected with long and chilly winters. The characterization study comprised 51 qualitative and 50 quantitative characters. Quantitative parameters were analyzed by Principal Components Analysis (PCA). The 2-dimensional plot (49.3% of cumulative variability) formed a main group of accessions and 4 outliers (#1, #9, #27 and #28) separated in the first PC. The characters with a higher contribution to the first PC were inflorescence length, leaf petiole length and leaf central foliole area. The presence of alkaloids, the percentage of plants killed by soil borne fungal diseases, the phenology, the yield per plant and yield components were also assessed. Twenty six accessions (the main group in the PCA plot) showed high homogeneity, with the following highlights: bitterness, indeterminate growth habit, early ripeness and spring sowing with the exception of #18, in which seeding date is unknown and it had a later phenology. By contrast accessions #9 and #27 were bitter of autumn sowing with determinate growing habit and they had the longest values of primary inflorescence length and a later phenology when they were sown in spring. Average values for yield and yield components showed a broad range of variation between accessions. The main yield component was the number of pods per plant. Accession #22 had the highest mean seed mass, although this had not significant influence in yield. Accession #17 showed the highest yield and #1 the lowest. This last also had the lowest values of inflorescence length, leaf petiole length and leaf central foliole area. No significant differences of resistance to soil-borne fungi were found between accessions. The year had a significant effect in the phenology, yield and yield components except for number of seeds per pod. The studied material might be of interest for the development of spring sowing germplasm adapted to north Iberian peninsula.  相似文献   
76.
Nickel adsorption onto a sludge wasting from a biological aeration tank is described in this work. This procedure is planned as an alternative method for metal removal from industrial effluents containing heavy metals in low concentrations. Experimental results of equilibrium adsorption and laboratory equipment operation are reported. Equilibrium data of nickel adsorption were fitted to the Freundlich equation. On the basis of this equation, the effect of several parameters such us metal concentration, sludge concentration and metal dosing rate were tested. Nickel removal efficiency is about 80% in the most favourable cases. Aeration during metal-sludge contact was also tested but little enhancing in metal uptake was observed. This analysis can be used to define the best operation conditions in practical cases either for metal or organic matter removal.  相似文献   
77.
Three isoforms of the cDNA of the major 8S globulin of mungbean, 8Salpha, 8Salpha', and 8Sbeta, were isolated, cloned, and characterized. The cDNA sequences of 8Salpha, 8Salpha', and 8Sbeta had open reading frames of 1362, 1359 or 1362, and 1359 bp, respectively, which code for 454, 453 or 454, and 453 amino acids corresponding to molecular weights of 51 973, 51 627 or 51 758, and 51 779, respectively. Homology in terms of cDNA and amino acid sequences was 91-92% between 8Salpha and 8Salpha', 87% between 8Salpha and 8Sbeta, and 86-88% between 8Salpha' and 8Sbeta. The signal peptide was found to be 1-25, 1-24 or 25, and 1-23 for 8Salpha, 8Salpha', and 8Sbeta, respectively, using the signalP website (Nielsen, H.; Engelbrecht, J.; Brunak, S.; von Heijne, G. Protein Eng. 1997, 10, 1-6). The propeptide was determined to be IVHREN. A single site for glycosylation (N-X-S/T) was observed about 90 amino acids from the C terminus. Homology between mungbean 8S isoforms and other 7-8S proteins ranged from 45 to 68% within members of the legume family and 29 to 34% for crops of different species. The major isoform 8Salpha was expressed in Escherichia coli and purified by successive ammonium sulfate fractionation, hydrophobic interaction, and Mono Q column chromatography. The recombinant 8Salpha, but not the native form, was successfully crystallized producing rhombohedral crystals.  相似文献   
78.
Empirical, statistically based models were used to describe the growth and development of Eucalyptus nitens plantations for a range of site productivities and the standard biomass and pulp silvicultural regime currently applied in Northern Spain. The results obtained, along with data gathered from a network of 68 plots, 48 trees felled for biomass estimations and 73 trees sampled for foliar area estimation were used to parameterize the 3-PG model for this species in Northern Spain. Most parameters associated with allometric relationships and partitioning (i.e. bark and branch fraction, basic density, age modifier and mortality) were derived from local data, and the remaining parameters were obtained from published studies on E. nitens or default values previously used for E. globulus. The parameterized model was validated with data from three trials measured from age 3 years until age 8-14 years, and performed better than the empirical model in terms of total stand under bark volume, mean diameter at breast height, basal area and foliar biomass. The process-based model was then used to forecast changes in plantations subjected to a clearwood regime, initializing the model at age 3 years, considering 3 prunings, 2 thinnings and lengthening the rotation to 18 years. This integrated regime was able to provide biomass for bioenergy, pulp or fibreboard wood and also solid wood, with thinning operations assisting the financial viability, and was a potentially good alternative for productive sites.  相似文献   
79.

Objective  

Knowledge of mechanisms determining plant persistence in range edges is essential because peripheral populations face increasing risk and they often maintain unique genotypes.  相似文献   
80.
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