Combined treatment with oestradiol benzoate,d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes     

Jeferson Ferreira da Fonseca  Fabiana Nunes Zambrini  Jos Domingos Guimares  Marcio Roberto Silva  Maria Emilia Franco Oliveira  Felipe Zandonadi Brando  Pawel Mieczyslaw Bartlewski  Joanna Maria Gonalves Souza‐Fabjan 《Reproduction in domestic animals》2019,54(1):118-125

This study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous‐synchronized Santa Inês ewes pretreated with d‐cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.  相似文献   

Cervical transposition test using Hegar dilator at oestrus as a tool to select ewes for transcervical embryo collection     

Juliana Dantas Rodrigues Santos  Eduardo Kenji Nunes Arashiro  Mrio Felipe Alvarez Balaro  Joanna Maria Gonalves Souza‐Fabjan  Pedro Henrique Nicolau Pinto  Clara Vieira de Souza  Ceci Ribeiro Leite  Jeferson Ferreira da Fonseca  Felipe Zandonadi Brando 《Reproduction in domestic animals》2019,54(1):126-128

This study evaluated the cervical transposition method as a tool to select ewes for embryo collection by transcervical route. Adult Santa Inês ewes (n = 50) received Day 0 protocol for superovulation treatments. The cervix transposition test was performed both at oestrus and at the embryo collection time. The latter was preceded by hormonal cervical dilation. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 85.7%, 66.6%, 85.7%, 66.6% and 80.0%, respectively. The kappa index yielded a moderate score (κ = 0.52). In conclusion, the high sensitivity and accuracy indicate that the cervical transposition test is a screening option to select ewes for embryo collection by transcervical route.  相似文献   

Soybean grain is a suitable replacement with soybean meal in multiple supplements for Nellore heifers grazing tropical pastures     

Daniel Mageste de Almeida  Marcos Inácio Marcondes  Luciana Navajas Rennó  Leandro Soares Martins  Faider Alberto Castaño Villadiego  Mário Fonseca Paulino 《Tropical animal health and production》2018,50(8):1843-1849

The research aimed to evaluate the effect of replacing soybean meal with soybean grain on the nutritional parameters and productivity of heifers grazing on Urochloa decumbens in the rainy-dry transition period. Forty crossbred heifers with the initial age and weight of 18 months and 292?±?6.1 kg, respectively, were used. The experimental design was a completely randomized design, with five treatments and eight replications. The evaluated treatments were as follows: SM_0.5—supply of 0.5 kg/animal/day of soybean meal supplement; SG_0.5—supply of 0.5 kg/animal/day of soybean grain supplement; SM_1.0—supply of 1.0 kg/animal/day of soybean meal supplement; SG_1.0—supply of 1.0 kg/animal/day of soybean grain supplement; MM—only mineral mix ad libitum. The supplement was isoprotein with 350 g of crude protein/kg of dry matter. Supplementation improved the performance of the heifers and this fact can be verified by the higher average daily gain (ADG) and final BW (fBW) of the supplemented animals (P?<?0.10). The two supplemented treatments with 1 kg/day demonstrated similar performance (P?>?0.10), the same happens for the two treatments receiving 0.5 kg/day (P?>?0.10). However, animals receiving 1 kg/day of supplementation had an ADG and final BW higher than animals receiving 0.5 kg/day (P?<?0.10). Supplementation (P?<?0.10) affected the intake of dry matter (DM), organic matter, crude protein, ether extract, non-fiber carbohydrates, total digestible nutrients, and neutral detergent fiber corrected for ash and protein (apNDF). Supplementation improved DM digestibility and all constituents of the diet (P?<?0.10), except for apNDF (P?>?0.10). In summary, it is concluded that multiple supplementations improve the performance of grazing heifers in the rainy-dry transition period and the total replacement of soybean meal by soybean grain does not alter the performance of the animals.  相似文献   

Estimation of daily milk yield of Nellore cows grazing tropical pastures     

Daniel Mageste de Almeida  Marcos Inácio Marcondes  Luciana Navajas Rennó  Lívia Vieira de Barros  Carla Heloísa Avelino Cabral  Leandro Soares Martins  David Esteban Contreras Marquez  Felipe Vélez Saldarriaga  Faider Alberto Castaño Villadiego  Manuela Acevedo Cardozo  Roman Maza Ortega  Javier Enrique Garces Cardenas  Virginia Lucia Neves Brandão  Mário Fonseca Paulino 《Tropical animal health and production》2018,50(8):1771-1777

Beef cows’ milk yield is typically determined by measuring milk yield once daily and then doubling this value to estimate daily production. However, it is not known whether this is accurate. Thus, we aimed to determine the association between morning and afternoon milk yield in grazing Nellore cows. Eighty Nellore cows were used, with initial weight of 516.0?±?1.0 kg. The experiment was a completely randomized factorial scheme, with 20 replications and four treatments (i.e., +?or ??pre-partum supplementation in combination with +?or ??post-partum supplementation): PRMM—1 kg of supplement/cow/day for 90 days pre-partum; MMPS—1 kg of supplement/cow/day for 90 days post-partum; PRPS—1 kg of supplement/cow/day for 90 days pre-partum and 90 days post-partum; and MM—only mineral mix ad libitum during pre- and post-partum. Milk was sampled on days 45, 135, and 225 post-partum (early, middle, and late lactation, respectively). No effects were observed of pre- and post-partum supplementation on milk yield (P?>?0.05). The afternoon/morning proportion of 0.45 in the early third of lactation was higher than other stages, which had a proportion of 0.41 (P?<?0.05). Post-partum supplementation increased milk protein in the morning and afternoon milking (P?<?0.05). There was also no effect of pre- and post-partum supplementation on afternoon-morning proportion other milk components (P?>?0.05). We conclude that estimating daily milk production of grazing beef cattle by multiplying a once daily milking amount times two is not accurate. Under the conditions of this study, proportion of total daily production represented by the ratio of afternoon/morning milking was 0.45 in early lactation (first third) and 0.41 in mid- and late lactation.  相似文献   

Epidemiological investigation of <Emphasis Type="Italic">Borrelia burgdorferi</Emphasis> in horses in the municipality of Sinop—MT,Brazil     

Suyane Nayara Garcia Socoloski  Bruno Gomes de Castro  Matheus Dias Cordeiro  Adivaldo Henrique da Fonseca  Marcio Barizon Cepeda  Rafael Romero Nicolino  Luciano Bastos Lopes 《Tropical animal health and production》2018,50(4):831-836

Borrelia burgdorferi sensu stricto is the main etiological agent of Lyme disease (LD) in the USA. In Brazil, it is believed that a similar spirochete is the causal agent of the Baggio–Yoshinari syndrome (BYS), a zoonosis also transmitted by ticks, whose clinical manifestations are similar to those of LD. Despite the epidemiological importance, there are no studies reporting the presence and the prevalence of B. burgdorferi among horses in Mato Grosso State. The aim of this study was to detect and measure the frequency of IgG antibodies anti-B. burgdorferi American strain G39/40 in horses in the municipality of Sinop, MT—Brazil, using the indirect enzyme-linked immunosorbent assay (ELISA) for serological diagnosis. Blood samples from 367 horses were collected in 81 farms. An epidemiological questionnaire was applied during the visits to obtain information related to the animals and the farms. From the 367 horses, 214 were positive for B. burgdorferi sensu stricto according to the results of the ELISA test, representing an apparent prevalence of 54.04% [CI?=?0.4548051–0.6237234]. Concomitantly, 89 blood samples were taken for molecular analysis by nested polymerase chain reaction (PCR). According to the PCR test results, none of the samples were reactive, although 53 of these samples were reactive according to ELISA. Seventy five farms (92.59%) had at least one reactive horse for B. burgdorferi. Our results support the hypothesis of the presence of anti-Borrelia spp. antibodies in horses in Mato Grosso, reaching a high animal prevalence. Besides that, leisure/sport purposes proved to be a risk factor, with an odds ratio of 3.16. These findings clearly indicate the need of borreliosis control in Sinop and make a significant contribution to the knowledge of the disease in Mato Grosso.  相似文献   

Pathogenicity of Ostertagia circumcincta,O. ostertagi and H. contortus in weanling stag fawns (Cervus elaphus)     

JT Johnson  AS Familton  R McAnulty  AR Sykes 《New Zealand veterinary journal》2013,61(12)

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Photosynthetic capacity of leaves of Eucalyptus globulus (Labill.) growing in the field with different nutrient and water supplies     

Pereira JS  Chaves MM  Fonseca F  Araújo MC  Torres F 《Tree physiology》1992,10(4):381-389

A technique was tested for obtaining water relations parameters from individual fascicles of loblolly pine needles by the pressure-volume curve method. Comparisons were made between parameters derived from (1) individual fascicles that were rehydrated in a pressure chamber after being removed from the shoot and (2) fascicles that were rehydrated on the shoot. Estimates of tissue osmotic potential for needles rehydrated by the two methods were significantly different for needles from terminal shoots, but not significantly different for needles from lateral shoots. Similarly, a significant difference in the estimated tissue elasticity at zero water potential was noted for needles on terminal shoots, but not for those on lateral shoots. It is suggested that differences due to rehydration method are related to the duration of the rehydration period and not the choice of technique. The use of fascicles of needles, rehydrated after detachment, allows repeated estimation of the tissue water relations of a single conifer shoot.  相似文献   

Variable very-high-energy gamma-ray emission from the microquasar LS I +61 303     

Albert J  Aliu E  Anderhub H  Antoranz P  Armada A  Asensio M  Baixeras C  Barrio JA  Bartelt M  Bartko H  Bastieri D  Bavikadi SR  Bednarek W  Berger K  Bigongiari C  Biland A  Bisesi E  Bock RK  Bordas P  Bosch-Ramon V  Bretz T  Britvitch I  Camara M  Carmona E  Chilingarian A  Ciprini S  Coarasa JA  Commichau S  Contreras JL  Cortina J  Curtef V  Danielyan V  Dazzi F  De Angelis A  de Los Reyes R  De Lotto B  Domingo-Santamaría E  Dorner D  Doro M  Errando M  Fagiolini M  Ferenc D  Fernández E  Firpo R  Flix J  Fonseca MV  Font L 《Science (New York, N.Y.)》2006,312(5781):1771-1773

Microquasars are binary star systems with relativistic radio-emitting jets. They are potential sources of cosmic rays and can be used to elucidate the physics of relativistic jets. We report the detection of variable gamma-ray emission above 100 gigaelectron volts from the microquasar LS I 61 + 303. Six orbital cycles were recorded. Several detections occur at a similar orbital phase, which suggests that the emission is periodic. The strongest gamma-ray emission is not observed when the two stars are closest to one another, implying a strong orbital modulation of the emission or absorption processes.  相似文献   

Impact of the pinewood nematode, Bursaphelenchus xylophilus, on gross calorific value and chemical composition of Pinus pinaster woody biomass     

Valeria Reva  Luís Fonseca  José L. Lousada  Isabel Abrantes  Domingos X. Viegas 《European Journal of Forest Research》2012,131(4):1025-1033

The pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of the pine wilt disease, has been detected in several regions of Portugal affecting Pinus pinaster, a coniferous species of a great economic value. The nematodes, migrating through resin canals and feeding on parenchyma cells, induce rapid metabolic changes in ray parenchyma cells, cavitation areas, and denaturation and necrosis of parenchyma and cambial cells. To understand how anatomic changes and biochemical incidences of tree defense reactions affect the technological parameters of the wood, the gross calorific value (GCV) and chemical composition of PWN-infected and -uninfected P. pinaster wood were evaluated. The GCV was determined using Parr 6300 Automatic Isoperibol Calorimeter, and chemical composition analysis was performed by determining the contents of carbon, hydrogen, nitrogen, sulfur and Ash on complete and instant oxidation of samples by “flash” combustion. The Student’s t test with Welch correction was used for statistical data analysis. The difference between the GCV and chemical composition for PWN-infected and -uninfected P. pinaster wood was statistically significant for the GCV and for hydrogen and nitrogen contents. The carbon, oxygen, sulfur and Ash contents did not differ statistically. The GCV of PWN-infected wood varied between the highest value of hardwood and the lowest value of softwood. This interdisciplinary study stresses the important technological and economic aspects, namely the impact of PWN on wood properties and the suitability of infected P. pinaster wood for use in the wood-processing and energy industries.  相似文献   

Cumulus cell and oocyte mRNA expression of hormone and growth factor receptors in mares     

Aurea Wischral  Manoel Adriao  Keith T. Haag  Gabriela R. Fonseca  Melba O. Gastal  Eduardo L. Gastal 《Journal of Equine Veterinary Science》2012

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