Changes in fatty acid and sterol composition during oogenesis in the pearl oyster Pinctada margaritifera     

Isabelle Vahirua‐Lechat  Frédéric Laure  Jean René LeCoz  Jean Pierre Bianchini  Mereani Bellais  Gilles Le Moullac 《Aquaculture Research》2008,39(16):1739-1746

The fatty acid and sterol composition of the oyster Pinctada margaritifera during oogenesis and in eggs was analysed. No major differences were observed during oogenesis, but the egg composition was significantly different from that of gonads. The amount of saturated fatty acids was the highest in eggs and the C16:0 predominant (P<5%); by contrast, the amount of 22:6(n‐3) was significantly lower (P<5%) than in gonads. No major differences were observed for the polar lipid (PL) composition during oogenesis. The main free sterols in gonads and eggs were cholesterol and brassicasterol. Among free sterols, the proportion of cholesterol diminished continuously from the beginning to the end of gonad maturation, and this decrease persisted in eggs after spawning. Cholesterol represented 40% to 55% of the sterol ester encountered in gonad and eggs. This study allowed us to investigate the fatty acid and sterol composition during oogenesis of the pearl oyster P. margaritifera, leading to a clearer understanding of the nutritional requirements of pearl oyster during the reproduction process.  相似文献   

Complete Larval Development of the Fresh Water Prawn Macrobrachium vollenhovenii in Cameroon     

Judith G. Makombu  Pius M. Oben  Benedicta O. Oben  Guillaume L. P. Gaudin  Isabelle S. Motto  Ndelle Makoge 《Journal Of Applied Aquaculture》2013,25(4):310-328

The African River prawn Macrobrachium vollenhovenii is the largest of the local Palaemonidae prawns along the Cameroonian coast and an important target species for fisheries and aquaculture. A preliminary study on larviculture of this species was carried out at the IRAD-AQUASOL hatchery in Kribi, Cameroon. Berried females M. vollenhovenii were obtained from the Lobe and Lokoundje Rivers in the Southern Region, typical Lower Guinea rainforest streams. The larval rearing system consisted of a single recirculation system of four 65 L cylindrical plastic containers connected to a submerged biological filter container (65 L). Experiments were conducted three times in the same condition, with two batches of larvae reared separately in duplicate per experiment, at a density of 40/L, temperature 26°C, and salinity 16%. Larvae were fed from 1 day after hatching until metamorphosis to postlarvae (PL) with newly hatched Artemia nauplii, three times daily at the rate of 5 nauplii per mL water. The number of newly hatched larvae per female body weight unit was not affected by the female size and ranged between 531 to 1349 larvae g^?1. The six batches succeeded from hatching to metamorphosis, and the 11 distinct larval stages described for M. rosenbergii were found. Larvae in all the batches developed more or less at the same pace up to stage V. However, a clear difference in the timing of appearance of the developmental stages was observed between batches and individuals of the same batch during the subsequent stages. The transition from stage V to stage VI and from stage IX to stage X was longer than the passage to other stages and seems to be the critical rearing period of M. vollenhovenii. From day 20 onward to sampling, the larval stage index (LSI) showed that larval development of the batch 1 was significantly faster than for all other batches. The time for first appearance of postlarvae was variable between batches (41–74 days), and batch 1 larvae passed through metamorphosis within a shorter span of time (41 days). Survival up to postlarvae was variable among batches, ranging between 3%–9%, and was better in batch 1 (9.31 ± 1.09). The general poor performance in terms of survival and metamorphosis rates of larvae seems to be related to the wild condition of broodstock. To improve performance of larvae, domestication of stock may be necessary. This result is the first recorded success in larviculture of M. vollenhovenii in Cameroon.  相似文献   

Consumer perception versus scientific evidence of farmed and wild fish: exploratory insights from Belgium   总被引：2，自引：2，他引：0  

Wim Verbeke  Isabelle Sioen  Karen Brunsø  Stefaan De Henauw  John Van Camp 《Aquaculture International》2007,15(2):121-136

The increasing number of marketable fish being supplied from aquaculture is a response to the increasing demand for healthy food and is filling the gap left by depleting natural fish stocks. Little is known about the awareness and perception of the consumer in terms of farmed fish versus fish from capture fisheries. The consumer's subjective point of view is of overriding importance for the production system and product acceptance as well as for future market success. In this paper consumer perception in Belgium is explored and compared against scientific evidence of farmed versus wild fish. Primary data were collected through a consumer survey (April 2003) and focus group discussions (May 2004) with Belgian consumers. The majority of the consumer sample reported no perceived differences between farmed versus wild fish. However, mean perception scores were slightly in favour of wild fish on the attributes taste, health and nutritious value, in particular among consumers aged 55 years and older. The availability of farmed fish was perceived to be better than that of wild fish, while the consumer's perception of safety did not differ between farmed and wild fish. The focus group discussions indicated that consumers’ opinions and beliefs about farmed fish are mainly based on emotion and image transfer from intensive terrestrial livestock production rather than on awareness and factual knowledge of aquaculture.

John Van CampEmail:

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From shallow to deep waters: habitats used by larval lampreys (genus Petromyzon and Lampetra) over a western European basin     

Catherine Taverny  Isabelle Ortusi  Charles Roqueplo  Mario Lepage  Patrick Lambert 《Ecology of Freshwater Fish》2012,21(1):87-99

Abstract –  Habitat requirements of lamprey ammocoetes (Petromyzon marinus and Lampetra genus) were investigated, for the first time, from shallow to deep waters, at different spatial scales across the Gironde‐Dordogne continuum, thanks to a water suction dredge. Fish‐habitat relationships were assessed through two complementary statistical analyses: habitat‐use curves and habitat suitability models using the Boosted Regression Trees (BRT) technique. Analyses were performed on a small‐size data set that was characterised by the low prevalence of lamprey. The sea lamprey larvae occurred in deeper areas than their Lampetra genus counterparts. ‘Pools’ of 2 m’ depth and more were optimal habitats for the former species. Among the environmental variables retained to model lamprey occurrences, the mesohabitat (a categorical variable) was demonstrated to be highly influential, in terms of fine grain‐size substratum and vegetation cover. These preliminary results suggest that monitoring using the water suction dredge method may contribute to sea lamprey conservation.  相似文献   

Monitoring of atrazine treatment on soil bacterial,fungal and atrazine-degrading communities by quantitative competitive PCR     

Martin-Laurent F  Piutti S  Hallet S  Wagschal I  Philippot L  Catroux G  Soulas G 《Pest management science》2003,59(3):259-268

We report the development of quantitative competitive (QC) PCR assays for quantifying the 16S, 18S ribosomal and atzC genes in nucleic acids directly extracted from soil. QC-PCR assays were standardised, calibrated and evaluated with an experimental study aiming to evaluate the impact of atrazine application on soil microflora. Comparison of QC-PCR 16S and 18S results with those of soil microbial biomass showed that, following atrazine application, the microbial biomass was not affected and that the amount of 16S rDNA gene representing 'bacteria' increased transitorily, while the amount of 18S rDNA gene representing fungi decreased in soil. In addition, comparison of atzC QC-PCR results with those of atrazine mineralisation revealed that, in response to atrazine treatment, the amount of atzC gene increased transitorily in soil pre-treated with atrazine, suggesting that accelerated atrazine biodegradation in soil could be due to a transient increase in the size of the atrazine mineralising community.  相似文献   

Healthy People 2010--new opportunities for veterinary medicine in the 21st century     

Hendrix CM  McClelland CL  Kahn KL  Thompson I  Pence PA 《Journal of the American Veterinary Medical Association》2002,221(7):951-957

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Spatial and temporal distribution of geminiviruses in leafhoppers of the genus cicadulina monitored by conventional and quantitative polymerase chain reaction     

Lett JM  Granier M  Hippolyte I  Grondin M  Royer M  Blanc S  Reynaud B  Peterschmitt M 《Phytopathology》2002,92(1):65-74

ABSTRACT Spatial and temporal distribution of Maize streak virus (MSV, family Geminiviridae, genus Mastrevirus) was monitored in the vector species Cicadulina mbila and the nonvector species C. china? using conventional and real-time quantitative polymerase chain reaction. Sustained feeding on MSV-infected plants showed that virus accumulation reaches a maximum in C. china?, but not in C. mbila. After a 3-day acquisition access feeding period (AAP), MSV was detected in the gut, the hemolymph, and the head of C. mbila, but only in the gut of C. china?. Similarly, Digitaria streak virus (genus Mastrevirus), which is not transmitted by either of the two species, was only detected in the gut. MSV was detected in the hemolymph of C. mbila 3 h after the beginning of the AAP. Although viral DNA progressively decreases in the vector and nonvector species after a 3-day AAP, MSV DNA remained stable in the salivary glands of C. mbila.  相似文献   

A Preliminary Study of Silver Sodium Zirconium Phosphate Polyurethane Foam Wound Dressing on Wounds of the Distal Aspect of the Forelimb in Horses          下载免费PDF全文

Maureen E. Kelleher DVM  Diplomate ACVS  Isabelle Kilcoyne MVB  Julie E. Dechant DVM  MS  Diplomate ACVS & ACVECC  Emma Hummer BS  Philip H. Kass DVM  PhD  Diplomate ACVPM  Jack R. Snyder DVM  PhD  Diplomate ACVS 《Veterinary surgery : VS》2015,44(3):359-365

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Transient diabetes mellitus in a domestic ferret (Mustela putorius furo)     

Alexis Duhamelle  Isabelle Langlois  Marion Desmarchelier 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(7):737-740

A 3.5-year-old spayed female ferret, fed a diet high in refined sugar, was referred for lethargy, polyuria, polydipsia, and polyphagia. Diabetic ketoacidosis was diagnosed. Treatment included insulin therapy and a low carbohydrate diet. Diabetes mellitus resolved 54 d later, and insulin therapy was discontinued. There has been no recurrence of the diabetes mellitus.  相似文献   

Green tea polyphenol extract regulates the expression of genes involved in glucose uptake and insulin signaling in rats fed a high fructose diet   总被引：1，自引：0，他引：1  

Cao H  Hininger-Favier I  Kelly MA  Benaraba R  Dawson HD  Coves S  Roussel AM  Anderson RA 《Journal of agricultural and food chemistry》2007,55(15):6372-6378

Green tea has antidiabetic, antiobesity, and anti-inflammatory activities in animal models, but the molecular mechanisms of these effects have not been fully understood. Quantitative real-time polymerase chain reaction (PCR) was used to investigate the relative expression levels and the effects of green tea (1 and 2 g solid extract/kg diet) on the expression of glucose transporter family genes (Glut1/Slc2a1, Glut2/Slc2a2, Glut3/Slc2a3, and Glut4/Slc2a4) and insulin signaling pathway genes (Ins1, Ins2, Insr, Irs1, Irs2, Akt1, Grb2, Igf1, Igf2, Igf1r, Igf2r, Gsk3b, Gys1, Pik3cb, Pik3r1, Shc1, and Sos1) in liver and muscle of rats fed a high-fructose diet known to induce insulin resistance and oxidative stress. Glut2 and Glut4 were the major Glut mRNAs in rat liver and muscle, respectively. Green tea extract (1 g) increased Glut1, Glut4, Gsk3b, and Irs2 mRNA levels by 110, 160, 30, and 60% in the liver, respectively, and increased Irs1 by 80% in the muscle. Green tea extract (2 g) increased Glut4, Gsk3b, and Pik3cb mRNA levels by 90, 30, and 30% but decreased Shc1 by 60% in the liver and increased Glut2, Glut4, Shc1, and Sos1 by 80, 40, 60, and 50% in the muscle. This study shows that green tea extract at 1 or 2 g/kg diet regulates gene expression in the glucose uptake and insulin signaling pathway in rats fed a fructose-rich diet.  相似文献