Glycohistochemical characterization of histologically normal nasal mucosa and enzootic nasal tumor of sheep     

P Scocco  E Lepri  F Mercati  G Vitellozzi  L Mechelli  P Ceccarelli 《American journal of veterinary research》2012,73(8):1128-1136

Objective-To determine glycohistochemical characteristics of enzootic nasal tumors (ENTs) of sheep, compare results for ENT with those of histologically normal nasal mucosa of sheep, and identify the histologic origin of ENT. Sample-ENT and nasal mucosa samples obtained from cadavers of 5 adult Lacaune sheep with ENT and 5 Lacaune sheep unaffected by ENT, respectively. Procedures-Samples of ENT and nasal mucosa were collected from cadavers of sheep and sectioned. Conventional and lectin histochemical analyses were used to identify glycoconjugates in tissue sections on the basis of their principal chemical groups and principal terminal or internal oligosaccharidic glucidic residues, respectively. Results-ENTs had papillary and tubular portions. Cells in the papillary portion of ENTs had secretion and surface glycoconjugates, which included sulfated glycosaminoglycans and neutral and sialilated glycoproteins. Cells in the tubular portion of ENTs had surface glycoconjugates, which included neutral and sialilated glycoproteins. Both portions of ENTs had C(4)-acetylated sialoderivatives that were not detected in sections of histologically normal nasal mucosa. Conclusions and Clinical Relevance-The papillary portion of ENTs in sheep may originate from respiratory glands and goblet cells. The tubular portion of ENTs in sheep may originate from olfactory glands. Presence of C(4)-acetylated sialoderivatives in cells of ENTs could confer resistance against pathogens to those cells.  相似文献   

Escherichia coli lipopolysaccharides and Staphylococcus aureus enterotoxin B differentially modulate inflammatory microRNAs in bovine monocytes     

Dilda F  Gioia G  Pisani L  Restelli L  Lecchi C  Albonico F  Bronzo V  Mortarino M  Ceciliani F 《Veterinary journal (London, England : 1997)》2012,192(3):514-516

MicroRNAs (miRNAs) are a family of regulatory molecules involved in many physiological processes, including activation of cells of the immune system. This study investigated the effect of Escherichia coli lipopolysaccharide (LPS) and Staphylococcus aureus enterotoxin B (SEB) on the expression of five miRNAs involved in the inflammatory response, including miR-9, miR-125 b, miR-155, miR-146 a and miR-223, in bovine CD14(+) cells (monocytes). Incubation of monocytes with SEB induced down-regulation of miR-155, miR-223 and miR-125 b, but not the anti-inflammatory miRNA miR-146 a. Conversely, incubation with LPS upregulated both miR-155 and miR-146 a. In vitro incubation of isolated CD14(+) bovine monocytes with LPS and SEB elicited different and opposite expression of miRNAs reportedly involved in inflammatory reactions.  相似文献   

Distribution of acute phase proteins in the bovine forestomachs and abomasum   总被引：1，自引：0，他引：1  

Dilda F  Pisani LF  Rahman MM  Modina S  Tessaro I  Sartorelli P  Ceciliani F  Lecchi C 《Veterinary journal (London, England : 1997)》2012,192(1):101-105

  相似文献   

Correlation Between Catechin Content and NF-κB Inhibition by Infusions of Green and Black Tea     

Chiara Di Lorenzo  Mario Dell’Agli  Enrico Sangiovanni  Ariana Dos Santos  Francesca Uberti  Enzo Moro  Enrica Bosisio  Patrizia Restani 《Plant foods for human nutrition (Dordrecht, Netherlands)》2013,68(2):149-154

  相似文献   

Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues     

Germán Cantón  Paul Bartley  Kathryn Bartley  Helen Todd  Francesca Chianini  Frank Katzer 《Veterinary immunology and immunopathology》2013,151(3-4):342-347

Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues.  相似文献   

In vitro adherence of Staphylococcus pseudintermedius to canine corneocytes is influenced by colonization status of corneocyte donors     

Narayan Chandra Paul  Francesca Latronico  Arshnee Moodley  S?ren Saxmose Nielsen  Peter Damborg  Luca Guardabassi 《Veterinary research》2013,44(1):52

The current knowledge of in vitro adherence of Staphylococcus pseudintermedius to canine corneocytes is limited to comparative analyses between strains, staphylococcal species or corneocytes collected from different breeds, body sites and hosts. However, the role played by colonization status of corneocyte donors remains unknown. The aim of this study was to evaluate the adherence properties of commensal S. pseudintermedius strains to corneocytes collected from dogs with different colonization status. For this purpose, corneocytes were collected from five dogs that were classified as persistently colonized (D1 and D2), intermittently colonized (D3 and D4) or non-colonized (D5) on the basis of the results of a previous longitudinal study. Adherence to corneocytes originating from each of the five dogs was assessed by an in vitro adhesion assay using four genetically unrelated strains isolated from the colonized dogs (S1 to S4). Irrespective of their host of origin, all strains adhered significantly better to corneocytes from D1 and D2 than to corneocytes from D3, D4 and D5 (P < 0.0001). The mean count of cells adhering to corneocytes from persistently colonized dogs was on average three times higher than the mean count using corneocytes from the other dogs. A significant difference between strains was only observed for one strain-corneocyte combination (S2-D4), indicating that S. pseudintermedius adherence to corneocytes is driven by host factors and only marginally influenced by strain factors. This finding has important implications for understanding and preventing S. pseudintermedius skin colonization and infection.  相似文献   

A case of pili torti in a young adult domestic short‐haired cat     

Elisa Maina  Silvia Colombo  Francesca Abramo  Gianandrea Pasquinelli 《Veterinary dermatology》2013,24(2):289-e68

  相似文献   

Evaluation of the combined effects of enzymatic treatment and aging on lees on the aroma of wine from Bombino bianco grapes     

Masino F  Montevecchi G  Arfelli G  Antonelli A 《Journal of agricultural and food chemistry》2008,56(20):9495-9501

In this study, two different doses of commercial beta-glucanase enzyme preparation were tested to verify their effect on wines aged on lees. These wines were compared with two samples with no enzymatic treatment. The former was aged on lees (control), and the latter was readily filtered off from the yeast cell biomass (standard). Analysis of variance (one-way ANOVA), the Tukey test, and principal component analysis (PCA) were applied to all of the samples, which were analyzed for aroma composition, along with galacturonic acid, total acidity, pH, and color. Results showed a large number of statistically significant differences among samples. In general, wines treated with beta-glucanase were characterized by higher concentration of many volatile compounds. The presence of lees and even more the exogenous enzymatic action enhanced almost all volatile compounds. Besides the high presence of ethyl esters, it is worth mentioning the behavior of hexanol and trans-3-hexenol, which are strongly enhanced by the presence of lees and by enzymatic treatments.  相似文献   

Cross-sectional study on acrylamide hemoglobin adducts in subpopulations from the European Prospective Investigation into Cancer and Nutrition (EPIC) Study     

Vesper HW  Slimani N  Hallmans G  Tjønneland A  Agudo A  Benetou V  Bingham S  Boeing H  Boutron-Ruault MC  Bueno-de-Mesquita HB  Chirlaque D  Clavel-Chapelon F  Crowe F  Drogan D  Ferrari P  Johansson I  Kaaks R  Linseisen J  Lund E  Manjer J  Mattiello A  Palli D  Peeters PH  Rinaldi S  Skeie G  Trichopoulou A  Vineis P  Wirfält E  Overvad K  Strömberg U 《Journal of agricultural and food chemistry》2008,56(15):6046-6053

Acrylamide exposure was investigated in subgroups of the EPIC study population (510 subjects from 9 European countries, randomly selected and stratified by age, gender, and smoking status) using hemoglobin adducts of acrylamide (HbAA) and its primary metabolite glycidamide (HbGA). Blood samples were analyzed for HbAA and HbGA by HPLC/MS/MS. Statistical models for HbAA and HbGA were developed including body mass index (BMI), educational level, and physical activity. A large variability in acrylamide exposure and metabolism between individuals and country groups was observed with HbAA and HbGA values ranging between 15-623 and 8-377 pmol/g of Hb, respectively. Both adducts differed significantly by country, sex, and smoking status. HbGA values were significantly lower in high alcohol consumers than in moderate consumers. With increasing BMI, HbGA in nonsmokers and HbAA in smokers decreased significantly. In the assessment of potential health effects related to acrylamide exposure, country of origin, BMI, alcohol consumption, sex, and smoking status should be considered.  相似文献   

A pilot study evaluating the effect of mannitol and hypertonic saline solution in the treatment of increased intracranial pressure in 2 cats and 1 dog naturally affected by traumatic brain injury     

Isabella Ballocco  Maria Antonietta Evangelisti  Roberta Deiana  Francesca Cubeddu  Maria Luisa Pinna Parpaglia  Giovanna Serra  Giovanni Carta  Maria Lucia Manunta 《Journal of Veterinary Emergency and Critical Care》2019,29(5):578-584

  相似文献