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排序方式: 共有80条查询结果,搜索用时 15 毫秒
31.
The presentation of approximately 40 dairy cows affected with left-side displacement of the abomasum (LDA) per annum in a cattle practice in East Gippsland, Victoria provided an opportunity to conduct a survey and case-control study of the disease in a grazing environment. The study, involving 37 dairy cows at pasture, revealed significant differences from the pattern of the disease occurring in the northern hemisphere where cows in older age groups, of larger frame size, higher production and fed high grain rations are at increased risk. Affected cows were diagnosed over a 10-month period and represented approximately 0.06% of the dairy cow population. Most cases were diagnosed in the early lactation period. Evidence for a genetic predisposition was suggested by the discovery that one sire generated a disproportionately large number (9) of the cows with LDA. Although affected cows were average producers in their herds, being a member of a high-producing herd was a significant risk factor. 相似文献
32.
Prostate vascular flow: The effect of the ejaculation on the power doppler ultrasonographic examination
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S Alonge M Melandri L Fanciullo GM Lacalandra G Aiudi 《Reproduction in domestic animals》2018,53(1):110-115
Power Doppler sonography (PD) can accurately depict tissue perfusion, recognize slow flows, and is relatively angle independent. The monitoring of local blood flow by Doppler ultrasonography is helpful in differentiating prostatic physio‐pathological conditions, but the recognizing of physiological variables that could affect it is crucial to apply this technique in clinical practice. This study aimed to evaluate if ejaculation affects blood flow to the prostate and to state how long this effect lasts. Serial PD examinations of prostate were performed in 18 dogs (1–5 years, 6–40 kg) immediately before (T0) and after (T1) the ejaculation, and repeated 6 (T2), 18 (T3) and 24 (T4) hours later. For each examination, two representative PD images were chosen and ranked by two independent observers according to the following scoring system: 0 = mild subcapsular (S) vascularization without clear evidence of parenchymal (P) vascularization; 1 = moderate P and S vascularization; 2 = severe S and moderate P vascularization; 3 = severe P and moderate S vascularization; 4 = severe P and S vascularization. Interobserver agreement was assessed using Kappa of Cohen. Ranked data, grouped according to time, were compared by ANOVA and Tukey HSD test (p < .05). Variations in the vascular flow pattern at different times were observed for all dogs. The statistical analysis evidenced a significant difference for T0 vs T1 and vs T2 and vs T3 (p < .01), with no significant difference for T0 vs T4 (p > .05). Interobserver agreement was very good (Kappa of Cohen = 0.86). This study demonstrated a definite increase in vascular flow to the prostate after ejaculation. The present results suggest a minimum of 24 hr sexual rest before the PD examination of the gland. This result should be taken into account whenever Doppler sonography is used to evaluate potential hyperaemia in dogs suspected of having prostate abnormalities. 相似文献
33.
姜大刚 《广西农业生物科学》2010,(1):160-163
水稻丝氨酸蛋白酶S8基因家族在水稻的生长发育过程中起着重要的调控作用。本研究利用公共数据库资源,分析水稻中丝氨酸蛋白酶S8基因家族,在水稻12条染色体上找到46个该类基因。通过其结构分析发现,每个基因的内含子数目从0到10各不相同,但氨基酸序列是非常保守的,都有催化活性位点和底物结合位点。系统进化树分析显示,这46个基因分为3个亚家族,S8-1亚家族最大。该家族基因的进化主要是通过基因重复复制的方式进行,其表达模式发生了变化,并且多个基因在穗部具有表达。 相似文献
34.
The clinical and anti-inflammatory effects of a single treatment of 0.4 mg meloxicam/kg bodyweight on pigs that had been challenged with Escherichia coli endotoxin were investigated. Significantly lower total clinical scores were recorded in pigs treated with meloxicam than in pigs treated with a placebo. Significantly higher mean serum concentrations of thromboxane B(2) were also recorded in pigs treated with a placebo for up to 24 hours after the challenge. The serum concentrations of acute phase proteins and specific antibody titres to E coli lipopolysaccharide were unaffected by the meloxicam. The meloxicam treatment was well tolerated. 相似文献
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The contribution of diatoms to bioflocs lipid content and the performance of juvenile Litopenaeus vannamei (Boone, 1931) in a BFT culture system
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Tatiana G Martins Clarisse Odebrecht Luciano V Jensen Marcelo GM D'Oca Wilson Wasielesky Jr 《Aquaculture Research》2016,47(4):1315-1326
This study aimed to evaluate the contribution of three diatom species on the lipid content of bioflocs, their permanence on the bioflocs and influence on the growth performance of juvenile shrimps. Juveniles of Litopenaeus vannamei were reared (30 days; three replicates per treatment) in biofloc systems inoculated with diatoms Amphora coffeaeformis (A), Cylindrotheca closterium (C), Conticribra weissflogii (W), or biofloc only (BF, chlorophycean rich). Water quality parameters were monitored daily and the microbiota on days 1, 10, 20 and 30. The lipid content and fatty acid profiles of bioflocs were analyzed at the end of the experiment. Shrimp survival rate (99%) at treatment A was significantly higher than at BF. The bioflocs in A treatment presented the highest lipid content, differing significantly from BF and W. The content of EPA (20:5) (n‐3) was significantly higher in A and lower in BF, while linoleic acid (18:2) (n‐6) was significantly higher in BF. The results indicate that high cell density of diatoms can be successfully maintained with silicate addition in biofloc systems and that the pennate A. coffeaeformis and the centric C. weissflogii are potentially better suited than the pennate C. closterium as food supplements for shrimp diets in biofloc nurseries system. 相似文献
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39.
In vitro production of porcine zygotes using intracytoplasmic injection of vitrified sperm
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CC Arraztoa C Baca Castex GM Alvarez PD Cetica DM Neild 《Reproduction in domestic animals》2017,52(5):775-780
The objectives of this study were to evaluate if vitrified porcine spermatozoa are able to maintain their capacity to produce zygotes in vitro using intracytoplasmic sperm injection (ICSI) and to evaluate the zygote development in two in vitro atmospheric conditions: 5% CO2 and tri‐gas. A group of porcine oocytes maturated in vitro were injected with vitrified‐warmed sperm (treatment group) and another group, with sperm diluted and conserved at 17°C (control group). To evidence parthenogenetic activation, some oocytes were submitted to a Sham test. The injected oocytes were cultured in G1 medium at 38°C, 100% humidity and 5% CO2 or tri‐gas. No significant differences (p > .05) were observed in embryo development between the oocytes injected with vitrified‐warmed sperm (31.8%; 36/113), and those injected with semen diluted and conserved at 17°C (35.5%; 32/90), when cultured in 5% CO2 or under tri‐gas atmosphere (42.9%; 39/91 vs. 34.2%; 26/76, respectively). No significant differences (p > .05) were observed in the percentage of pronuclei (PN) obtained between 5% CO2 and tri‐gas, within each treatment either. Of the 52 oocytes submitted to the Sham test, only two presented a female PN (activation) indicating that the PN observed in the treatment group were a product of fertilization and not parthenogenetic activation. To conclude, porcine sperm vitrified using spheres, at a concentration of 5 × 106 spermatozoa/ml in TALP medium with 1% bovine serum albumin (BSA), conserve condensed and intact chromatin capable of producing early embryo development up to the pronuclear stage. 相似文献
40.
SUMMARY: Psittacine beak and feather disease virus (PBFDV) was administered to adult galahs ( Eolophusroselcapillus ) by mouth or by intramuscular injection. Concentration of PBFDV antibodies in serum and excretion of PBFDV were monitored by haemagglutlnation inhibition (HI) and haemagglutination (HA) respectively. After oral administration, 17 of 18 galahs remained clinically normal and a small rise in antibody titre was detected in 3 of 18 birds. After intramuscular administration, antibody was detected in all birds. PBFDV was not detected in the feather dander of birds in either group. One bird developed diarrhoea and high faecal HA titres within 4 days of oral administration and then died. Adult and nestling cockatoos were vaccinated with an experimental inactivated double-oil emulsion vaccine. PBFDV antibody responses are comparable to those induced by a primary-oil emulsion vaccination regimen using Freund's adjuvants. Both vaccines protected nestlings. Three sibling wild-caught sulphur-crested cockatoos were vaccinated but died of PBFD before experimental challenge despite antibody responses in all birds. Unvaccinated control chicks developed acute PBFD within 4 weeks of challenge, probably from PBFDV-induced hepatitis since high concentrations of PBFDV were detected in their livers. 相似文献