Periplaneta americana peptide decreases apoptosis of pig-ovary granulosa cells induced by H2O2 through FoxO1     

Caihua Kong  Kena Liu  Qin Wang  Rong Fu  Huaxin Si  Shiyan Sui 《Reproduction in domestic animals》2021,56(11):1413-1424

Oxidative stress can induce apoptosis of granulosa cells and lead to follicular atresia, thereby reducing the number of pigs giving birth. The aim of this study was to investigate the protective effect of Periplaneta americana peptide (PAP) on the apoptosis of the granulosa cells of pig ovaries (PGCs) induced by hydrogen peroxide (H₂O₂) via FoxO1. PGCs were treated with H₂O₂ to establish a cell apoptosis model. Cell viability was measured using the cell counting kit-8 (CCK-8) assay, and cell apoptosis was detected using flow cytometry. The malondialdehyde (MDA) level and nitric oxide (NO) content were detected to reflect the oxidative stress. Western blotting, qRT-PCR and overexpression were undertaken to determine the expression of FoxO1 and caspase-3, and immunofluorescence was used to detect FoxO1 in the nucleus and cytoplasm. PGCs were treated with 100 μM H₂O₂ for 6 hr, which resulted in oxidative damage and apoptosis and an apoptosis rate for PGCs of 32.95%. Next, PGCs were treated with 400 μg/ml PAP for 24 hr to repair the apoptosis induced by H₂O₂. PAP improved cell viability in H₂O₂-stimulated PGCs, the increased MDA level and NO content caused by H₂O₂ stimulation were reversed and the apoptotic rate of PGCs was reduced. The qRT-PCR and Western blotting results indicated that PAP decreased the H₂O₂-induced apoptosis and the expression of FoxO1 and caspase-3 in PGCs. The effect of PAP was the same following FoxO1 overexpression. FoxO1 was expressed in the nucleus when stimulated by H₂O₂ or overexpression; however, it migrated to the cytoplasm following PAP treatment. PAP decreased the apoptosis of PGCs induced by H₂O₂ by regulating FoxO1 expression and nuclear translocation.  相似文献   

基于农作制分区的1985—2015年中国小麦生产时空变化   总被引：4，自引：0，他引：4  

BAI Bing  YANG Yu-Hao  WANG Xiao-Hui  JIA Hao  WU Yao  SHI Lei-Gang  YIN Xiao-Gang  CHEN Fu 《作物学报》2019,45(10):1554-1564

Comparing spatio-temporal variation characteristics of China’s wheat production, yield, sown area and yield-area-contribution in different farming zones in the past 30 years could help improving wheat planting layout and adjusting planting structure. Concentration index, rate of change, moving of gravity and resolution of yield-area-contribution were used to analyze spatio-temporal changes of China’s wheat production and yield-area-contribution based on county wheat production statistics including sown area, production and yield from 1985 to 2015. The wheat sown area decreased obviously in Northeast farming region, Northwest farming region and South farming region and increased rapidly in Huang-Huai-Hai farming region and Yangtze Plain farming region. In Huang-Huai-Hai farming region, the concentration indexes of Haihe Plain farming region, Huang-huai Plain farming region and Fenwei Basin farming region reached to 20.64%, 25.77%, and 21.65% respectively in 2015. Wheat production increased significantly by more than 48 milliontons in Huang-Huai-Hai farming region, and nearly 8 million tons Yangtze Plain farming region but decreased by more than 2.6 million tons in Northeast farming region. In Huang-Huai-Hai farming region, wheat production was concentrated in Haihe Plain farming region, Huang-huai Plain farming region and Yuxi Hill farming region. The average wheat yield continuously improved during the study period, Huang-Huai-Hai farming region and Northwest farming region had the yield increase up to 103.5 kg hm ^-2 and 92.9 kg hm ^-2 each year. Wheat yield in Yuxi Hill farming region, Fenwei Basin farming region and Haihe Plain farming region was relatively high in Huang-Huai-Hai farming region. The yield-reduced area was mainly caused by the decreasing of sown area, while the yield-area-contribution rate was different in yield-increased area. Yield-dominant counties were reduced, area-dominant counties were increased and yield-area-dominant counties were relatively steady. In production increased area, yield-dominant and yield-area-dominant counties were the main types in Huang-Huai-Hai Farming region, area-dominant and yield-area-dominant counties were the main types in Yangtze Plain farming region. Chinese wheat production was increasingly concentrated in Huang-Huai-Hai farming region which has high and rapid increase of wheat yield over the past three decades. Haihe Plain farming region, Huang-huai Plain farming region and Fenwei Basin farming region were the most concentrated areas in Huang-Huai-Hai farming region for wheat production during this period. Wheat yield and sown area jointly promoted the increase of wheat production in Huang-Huai-Hai farming region, wheat sown area was the crucial factor to increase wheat production in Yangtze Plain farming region, especially in the north of Jiangsu, Anhui province and greater part of Xinjiang.  相似文献   

1961—2010年东北农作区春小麦需水量的时空变化特征     

姜雨林  尹小刚  王猛  文新亚  田家瑞  胡惠杰  陈阜 《中国农业大学学报》2015,20(5):24-30

利用东北农作区58个气象站点1961—2010年的逐日气象数据,基于Simulation of Evapotranspiration of Applied Water(SIMETAW)模型分析气候变化背景下东北农作区春小麦生育期内作物需水量(Crop evapotranspiration,ETc)和灌溉需要量(Evapotranspiration of applied water,ETaw),以及典型站点春小麦的灌溉需求指数(Irrigation demand index,IDI)的时空变化特征。结果表明:近50年来,东北农作区日平均温度呈显著上升趋势,平均降水量下降趋势不明显,平均太阳辐射及作物蒸散量呈显著下降趋势。春小麦生育期作物需水量和灌溉需要量呈下降趋势,其分布均表现为西多东少。50年来春小麦作物生育期需水量下降主要集中于松辽及兴安岭南部地区,东部地区变化趋势不明显;生育期灌溉需要量下降集中于松辽及兴安岭地区,三江平原地区略有增加,长白山地区多年保持平稳。  相似文献   

Synergism between urea and urease-positive bacteria in controlling root-knot nematodes     

Fu Wen  Zhixiao Zhang  Yueqiu He  Zhen Chen  Ma Li  Minghe Mo 《European journal of plant pathology / European Foundation for Plant Pathology》2015,141(1):179-191

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日本百脉根LjbHLH34基因克隆及耐旱功能鉴定     

刘福  陈诚  张凯旋  周美亮  张新全 《草业学报》2023,32(1):178-191

干旱是影响植物生长发育的重要环境因素。本研究分析了日本百脉根抗旱相关基因LjbHLH34的耐旱功能，初步解析其响应干旱胁迫的分子机制，以期为百脉根抗旱分子育种提供理论基础。本研究克隆得到的LjbHLH34基因大小为711 bp、编码236个氨基酸，属bHLH转录因子家族成员。系统进化树分析显示，LjbHLH34蛋白与拟南芥bHLHⅣ亚家族中AtbHLH34和AtbHLH104亲缘关系较近。实时荧光定量分析表明LjbHLH34在日本百脉根的根中表达量最高，叶中次之，茎中最少，暗示其在日本百脉根多个组织中发挥作用；同时LjbHLH34基因也受聚乙二醇(PEG)和脱落酸(ABA)诱导表达。在酵母中检测发现LjbHLH34具有转录激活活性；亚细胞定位试验表明LjbHLH34蛋白定位于细胞核中。将LjbHLH34基因转入拟南芥获得过表达株系。在200 mmol·L^-1甘露醇胁迫下，LjbHLH34转基因拟南芥的根长明显长于野生型。干旱处理后，野生型拟南芥比转基因拟南芥萎蔫程度更加明显，而转基因株系的相对含水量和超氧化物歧化酶(SOD)活性显著高于野生型，丙二醛(MDA)积累...  相似文献   

“中国四库学高层论坛”会议综述     

刘艳伟  拓夫 《湖南农业大学学报(自然科学版)》2016,(6):38-40

2016年6月5-6日，由湖南大学岳麓书院主办的“中国四库学高层论坛”在岳麓书院举行，会议共收到论文45篇，与会专家学者围绕四库学研究的历史、《四库全书》的编纂、《四库总目》与学术、《四库总目》的考证与纠谬补缺及四库学的问题作了深入讨论。  相似文献   

四家动物园观赏肉食动物消化道寄生虫感染情况调查     

王宇  邹希明  张丽冰  付雪  宿欣  高远  王春仁 《黑龙江八一农垦大学学报》2017,29(1)

为了解哈尔滨北方森林动物园、佳木斯水源山公园、齐齐哈尔龙沙动植物园和鸡西动物园观赏肉食动物寄生蠕虫的感染情况,于2015年3~6月采用饱和盐水漂浮法和水洗沉淀法对四家动物园的5科94只观赏肉食动物的粪便进行了虫卵检查,结果有18只动物检出虫卵,阳性率为19.2%(18/94),蛔虫卵为常见虫种。本调查为四家动物园肉食动物消化道寄生虫病防治提供了科学依据。  相似文献   

Sulfadiazine pharmacokinetics in grass carp (Ctenopharyngodon idellus) receiving oral and intravenous administrations     

Ning Xu  Yu Fu  Fang Chen  Yongtao Liu  Jing Dong  Yibin Yang  Shun Zhou  Qiuhong Yang  Xiaohui Ai 《Journal of veterinary pharmacology and therapeutics》2021,44(1):86-92

This study aimed to examine the bioavailability (BA) and pharmacokinetic (PK) characteristics of sulfadiazine (SDZ) in grass carp (Ctenopharyngodon idellus) after oral and intravenous administrations. Blood samples were collected at predetermined time points of 0.083, 0.17, 0.5, 1, 2, 4, 8, 16, 24, 48, 72, and 96 hr (n = 6). The samples were extracted and purified by organic reagents and determined by the ultra‐performance liquid chromatography. The software named 3P97 was used to calculate relevant PK parameters. The results demonstrated that the concentration–time profile of SDZ was best described by a one‐compartmental open model with first‐order absorption after a single oral dose. The main PK parameters of the absorption rate constant (K_α), the absorption half‐life (t_{1/2 Kα}), the elimination rate constant (K_e), the elimination half‐life (t_1/2Ke), and the area under concentration–time profile (AUC_0‐∞) were 0.3 1/h, 2.29 hr, 0.039 1/h, 17.64 hr, and 855.78 mg.h/L, respectively. Following intravenous administration, the concentration–time curve fitted to a two‐compartmental open model without absorption. The primary PK parameters of the distribution rate constant (α), the elimination rate constant (β), the distribution half‐life (t_1/2α), the elimination half‐life (t_1/2β), the apparent distribution volume (V_SS), the total clearance (CL), and AUC_0‐∞ were 9.62 1/hr, 0.039 1/hr, 0.072 hr, 17.71 hr, 0.33 L/kg, 0.013 L h^?1 kg^?1, and 386.23 mg.h/L, respectively. Finally, the BA was calculated to be 22.16%. Overall, this study will provide some fundamental information on PK properties in the development of a new formulation SDZ in the future and is partially beneficial for the appropriate usage of SDZ in aquaculture.  相似文献   

Study of microRNA Expression Profile in Different Regions of Ram Epididymis     

Cuiling Wu  Chunxin Wang  Bo Zhai  Yunhui Zhao  Zhuo Zhao  Zhiyu Yuan  Mingxin Zhang  Kechuan Tian  Xuefeng Fu 《Reproduction in domestic animals》2021,56(9):1209-1219

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Effects of dietary Bacillus coagulans and yeast hydrolysate supplementation on growth performance,immune response and intestinal barrier function in weaned piglets     

Runqi Fu  Chan Liang  Daiwen Chen  Hui Yan  Gang Tian  Ping Zheng  Jun He  Jie Yu  Xiangbing Mao  Zhiqing Huang  Yuheng Luo  Junqiu Luo  Bing Yu 《Journal of animal physiology and animal nutrition》2021,105(5):898-907

The present study investigated the effects of Bacillus coagulans and yeast hydrolysate supplementation on growth performance, immune response and intestinal barrier function of weaned piglets. Twenty-four weaned piglets with an average body weight (BW) of 6.89 ± 0.15 kg were divided into four diets for 28 days. The treatments were basal diet (control), basal diet supplemented with antibiotic (20 mg/kg colistin sulphate and 40 mg/kg bacitracin zinc, AT), probiotics (400 mg/kg Bacillus coagulans ≥5 × 109 CFU/g, BC) or yeast hydrolysate (5000 mg/kg yeast hydrolysate, YH). Average daily gain (ADG) and average daily feed intake (ADFI) were improved by AT and YH diets (p < 0.05), while BC diet only increased ADG (p < 0.05). The complement 3 (C3), lysozyme (LZM) and tumour necrosis factor-α (TNF-α) concentrations in serum were increased in BC diet (p < 0.05). Feeding AT and YH caused the increase of jejunal villus height (p < 0.05), and a higher ratio of villus height/crypt depth was observed in AT, BC and YH groups (p < 0.05). The mRNA expression of zonula occludens-1 (ZO-1) in jejunal mucosa was up-regulated by AT, BC and YH diets (p < 0.05). Dietary AT, BC or YH inclusion decreased the interleukin-1β (IL-1β) concentration and TNF-α mRNA expression (p < 0.05), and YH supplementation even down-regulated toll-like receptor 4 (TLR4) and CD14 expressions (p < 0.05). In summary, the dietary administration of BC or YH both improves growth performance through promoting the intestinal barrier function, indicating both of them can serve as potential alternatives to antibiotics growth promoters for the piglet production.  相似文献