Finding functional features in Saccharomyces genomes by phylogenetic footprinting     

Cliften P  Sudarsanam P  Desikan A  Fulton L  Fulton B  Majors J  Waterston R  Cohen BA  Johnston M 《Science (New York, N.Y.)》2003,301(5629):71-76

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Prevalence of bovine viral diarrhea virus (BVDV) in persistently infected cattle and BVDV subtypes in affected cattle in beef herds in south central United States          下载免费PDF全文

Robert W. Fulton  Evan M. Whitley  Bill J. Johnson  Julia F. Ridpath  Sanjay Kapil  Lurinda J. Burge  Billy J. Cook  Anthony W. Confer 《Canadian journal of veterinary research》2009,73(4):283-291

The prevalence of bovine viral diarrhea virus (BVDV) in persistently infected (PI) cattle in beef breeding herds was determined using 30 herds with 4530 calves. The samples were collected by ear notches and tested for BVDV antigens using immunohistochemistry (IHC) and antigen capture enzyme-linked immunosorbent assay (ACE). Animals with initial positives on both IHC and ACE were sampled again using both tests and serums were collected for viral propagation and sequencing of a viral genomic region, 5′-untranslated region (5′-UTR) for viral subtyping. Samples were also collected from the dams of PI calves. There were 25 PI calves from 4530 samples (0.55%) and these PI calves were from 5 of the 30 herds (16.7%). Two herds had multiple PI calves and 3 herds had only 1 PI calf. Only 1 of the 25 dams with a PI calf was also PI (4.0%). The subtype of all the PI isolates was BVDV1b. Histories of the ranches indicated 23 out of 30 had herd additions of untested breeding females. Twenty-four of the 30 herds had adult cowherd vaccinations against BVDV, primarily using killed BVDV vaccines at pregnancy examination.  相似文献   

Gold nanoelectrodes of varied size: transition to molecule-like charging   总被引：1，自引：0，他引：1  

S Chen  RS Ingram  MJ Hostetler  JJ Pietron  RW Murray  TG Schaaff  JT Khoury  MM Alvarez  RL Whetten 《Science (New York, N.Y.)》1998,280(5372):2098-2101

A transition from metal-like double-layer capacitive charging to redox-like charging was observed in electrochemical ensemble Coulomb staircase experiments on solutions of gold nanoparticles of varied core size. The monodisperse gold nanoparticles are stabilized by short-chain alkanethiolate monolayers and have 8 to 38 kilodaltons core mass (1.1 to 1.9 nanometers in diameter). Larger cores display Coulomb staircase responses consistent with double-layer charging of metal-electrolyte interfaces, whereas smaller core nanoparticles exhibit redox chemical character, including a large central gap. The change in behavior is consistent with new near-infrared spectroscopic data showing an emerging gap between the highest occupied and lowest unoccupied orbitals of 0.4 to 0.9 electron volt.  相似文献   

Strong fisheries management and governance positively impact ecosystem status          下载免费PDF全文

Alida Bundy  Ratana Chuenpagdee  Jennifer L Boldt  Maria de Fatima Borges  Mohamed Lamine Camara  Marta Coll  Ibrahima Diallo  Clive Fox  Elizabeth A Fulton  Ayse Gazihan  Astrid Jarre  Didier Jouffre  Kristin M Kleisner  Ben Knight  Jason Link  Patroba P Matiku  Hicham Masski  Dimitrios K Moutopoulos  Chiara Piroddi  Tiit Raid  Ignacio Sobrino  Jorge Tam  Djiga Thiao  Maria Angeles Torres  Konstantinos Tsagarakis  Gro I van der Meeren  Yunne‐Jai Shin 《Fish and Fisheries》2017,18(3):412-439

Fisheries have had major negative impacts on marine ecosystems, and effective fisheries management and governance are needed to achieve sustainable fisheries, biodiversity conservation goals and thus good ecosystem status. To date, the IndiSeas programme (Indicators for the Seas) has focussed on assessing the ecological impacts of fishing at the ecosystem scale using ecological indicators. Here, we explore fisheries ‘Management Effectiveness’ and ‘Governance Quality’ and relate this to ecosystem health and status. We developed a dedicated expert survey, focused at the ecosystem level, with a series of questions addressing aspects of management and governance, from an ecosystem‐based perspective, using objective and evidence‐based criteria. The survey was completed by ecosystem experts (managers and scientists) and results analysed using ranking and multivariate methods. Results were further examined for selected ecosystems, using expert knowledge, to explore the overall findings in greater depth. Higher scores for ‘Management Effectiveness’ and ‘Governance Quality’ were significantly and positively related to ecosystems with better ecological status. Key factors that point to success in delivering fisheries and conservation objectives were as follows: the use of reference points for management, frequent review of stock assessments, whether Illegal, Unreported and Unregulated (IUU) catches were being accounted for and addressed, and the inclusion of stakeholders. Additionally, we found that the implementation of a long‐term management plan, including economic and social dimensions of fisheries in exploited ecosystems, was a key factor in successful, sustainable fisheries management. Our results support the thesis that good ecosystem‐based management and governance, sustainable fisheries and healthy ecosystems go together.  相似文献