鸡白痢沙门菌等位基因特异性PCR检测方法的建立     

耿士忠潘志明  蒋春红张辉李求春  焦新安 《中国兽医科技》2007,37(2):113-116

根据鸡白痢沙门菌与鸡伤寒沙门菌的rfbS基因在第237和第598位碱基的不同，设计和合成了等位基因特异性PCR引物，建立了快速检测鸡白痢沙门菌的PCR方法，并应用该方法对鸡白痢沙门菌临床分离样品进行了PCR鉴定。结果显示，该PCR方法能够特异性地鉴定鸡白痢沙门菌，检测灵敏度达100PgDNA。对35个经常规方法鉴定的鸡白痢沙门菌分离株应用等位基因特异性PCR方法进行鉴定，鉴定出33株鸡白痢沙门菌，符合率为94．3％。表明，建立的等位基因特异性PCR方法能够准确而快速地鉴定鸡白痢沙门菌。  相似文献   

番鸭呼肠孤病毒S基因组的克隆与序列分析     

耿宏伟  郭东春刘明  胡奇林张云 《中国兽医科技》2007,37(4):305-311

对番鸭呼肠孤病毒S12和S14毒株S基因组（S1-4）中σA、σB、σNS和σC蛋白基因进行了克隆和测序。序列分析表明：鸭呼肠孤病毒（DRV）与禽呼肠孤病毒（ARV）的σA和σNS基因的核苷酸同源性分别为76．0％～77．1％和78．4％～79．6％，氨基酸同源性分别为89．5％～91．2％和91.6％～92．7％；而具有诱导群特异性和型特异性中和抗体的σB和σC基因的核苷酸同源性分别为60．3％～64．4%和2．7%～9．9％，氨基酸同源性分别为61．4％～62．0％和22．6％～26．7％；DRV和ARV抗原性存在差异。而DRVS12／S14与法国89026株σA、σB、σNS和σC基因的核苷酸同源性分别为90．0％、93．6％、87．9％～88．0％和93．1%，氨基酸同源性分别为97．1%、94．3%、95．7%～95．9%和93．7％。进化树分析表明，DRV与ARV形成不同的分支，DRV是正呼肠孤病毒属中不同于ARV的一种新的呼肠孤病毒。  相似文献   

斑点叉尾(鮰)嗜麦芽寡养单胞菌的分离鉴定及系统发育分析   总被引：1，自引：0，他引：1  

耿毅  汪开毓  陈德芳  黄小丽 《中国兽医学报》2007,27(3)

从发生在四川、重庆等省市的斑点叉尾(鮰)急性流行性传染病病鱼的肝脏、肾脏内分离到1株高致病性菌株(CCF00024),人工感染健康鱼表现出与自然病鱼相同的症状,并从中分离获得同种细菌,证实其为斑点叉尾(鮰)急性流行性传染病的病原菌.形态、生理生化检测表明,该菌为非发酵型直杆菌,严格需氧,革兰氏阴性,极生多鞭毛,对除麦芽糖和甘露糖以外的多种糖类不利用产酸,氧化酶阴性,DNA酶、蛋白酶、脲酶、赖氨酸脱羧酶阳性,MR、VP阴性.在以该菌16S rDNA序列(GenBank登录号AY970826)和GenBank及RDP数据库内同源性较高的细菌16S rDNA序列构建的系统发育树中,分离菌CCF00024与嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)聚在一簇,特别是与S.maltophilia M5-1的同源性最高,序列相似性达99.6%,结合形态和生理生化特点将其鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia).药敏试验结果表明,对磺胺甲(口恶)唑、磺胺异(口恶)唑、阿齐霉素、洛美沙星高度敏感,而对新霉素、卡那霉素、氨苄青霉素,头孢唑啉、先锋霉素V和链霉素不敏感.  相似文献   

Effect of Supplementing Alfalfa Powder to Diet on Growth Performance and Meat Quality of Goose     

YAN Chunchi  DONG Biao  ZHAO Minmeng  LIU Long  GENG Tuoyu  GONG Daoqing  WANG Jian 《中国畜牧兽医》2007,47(11):3502-3509

The experiment was aimed to investigate the effects of dietary alfalfa powder on the growth performance,body size,slaughter performance,organ index and meat quality of Siji geese.A total of 256 15-day-old geese (half male and half female) with similar body weight were randomly divided into four groups with 4 replicates of 16 geese each.The geese in control group were fed with corn-soybean meal-based diet (control diet),and geese in the experimental groups were fed the basal diet replaced with 10%,20% and 30% alfalfa powder,respectively.The results showed that,compared with the control group,dietary alfalfa powder supplementation had no significant effect on the average daily intake,average daily gain,feed conversion ratio,body oblique length,keel length,shin circumference,semi diving length,chest width and shin length (P>0.05),and also had no effect on the carcass ratio,half-eviscerated carcass ratio and pectoral muscle ratio (P>0.05).However,dietary 20% and 30% alfalfa powder supplementation significantly increased the chest depth (P<0.05),dietary 30% alfalfa powder significantly decreased the full-eviscerated carcass ratio (P<0.05),and dietary 10% alfalfa powder significantly increased the leg muscle ratio (P<0.05) of geese.In addition,dietary alfalfa powder significantly decreased the abdominal fat ratio (P<0.05),raised the growth index of liver,jejunum,ileum,and cecum (P<0.05),and increased the water loss rate of pectoral muscle and leg muscle (P<0.05) compared with the control group.Dietary 10% and 30% alfalfa powder significantly increased duodenal growth index (P<0.05) of geese.In conclusion,dietary alfalfa powder supplementation might improve the slaughter performance (increase chest depth and leg muscle ratio,reduce abdominal fat rate) and organ growth index (increase the growth index of liver,duodenum,jejunum,ileum and cecum).However,dietary alfalfa powder increased water loss rates in pectoral muscle and leg muscle,and 30% alfalfa powder reduced the full-eviscerated carcass ratio of geese.The appropriate supplementation level of alfalfa powder was recommended at 10% in the present study.  相似文献   

斑点叉尾鲴嗜麦芽寡养单胞菌的分离鉴定及系统发育分析     

耿毅汪开毓  陈德芳黄小丽 《中国兽医学报》2007,27(3):330-335

从发生在四川、重庆等省市的斑点叉尾妇急性流行性传染病病鱼的肝脏、肾脏内分离到1株高致病性菌株（CCF00024），人工感染健康鱼表现出与自然痛鱼相同的症状，并从中分离获得同种细菌，证实其为斑点叉尾鲴急性流行性传染病的病原菌。形态、生理生化检测表明，该菌为非发酵型直杆菌，严格需氧，革兰氏阴性，极生多鞭毛，对除麦芽糖和甘露糖以外的多种糖类不利用产酸，氧化酶阴性，DNA酶、蛋白酶、脲酶、赖氨酸脱羧酶阳性，MR、VP阴性。在以该菌16SrDNA序列（GenBank登录号AY970826）和GenBank及RDP数据库内同源性较高的细菌16SrDNA序列构建的系统发育树中，分离菌CCF00024与嗜麦芽寡养单胞菌（Stenotrophomonasmaltophilia）聚在一簇，特剐是与5．maltophiliaM5—1的同源性最高，序列相似性达99．6％，结合形态和生理生化特点将其鉴定为嗜麦芽寡养单胞菌（Stenotrophomonasmaltophilia）。药敏试验结果表明，对磺胺甲口恶唑、磺胺异口恶唑、阿齐霉素、洛美沙星高度敏感，而对新霉素、卡那霉素、氨苄青霉素、头孢唑啉、先锋霉素V和链霉素不敏感。  相似文献   

白灵菇15号杂交菌株选育研究   总被引：1，自引：2，他引：1  

刘宇  耿小丽  王守现  孟莉莉 《食用菌学报》2006,13(3):13-18

以白灵菇4号菌株和10号菌株为亲本，通过单孢杂交育种技术选育出白灵菇15号杂交新菌株。白灵菇15号杂交新菌株的子实体呈手掌形，柄较短，产量较高，平均生物学效率达48．26％。  相似文献   

养鹿生产管理信息系统的设计   总被引：1，自引：0，他引：1  

耿忠诚  贾永全  叶建敏 《黑龙江畜牧兽医》2000,(4):38-39

目前我国一般养鹿场和专业户 ,普遍存在信息比较闭塞 ,处理信息的方法比较原始、落后 ,与当今时代的要求差距甚远 ,使养鹿生产的管理工作不能做到决策科学、处理及时、组织严密、检测控制和协调有效。因此 ,有必要开展养鹿生产信息管理方面的研究 ,开发相关的计算机应用软件 ,以帮助生产管理者和决策者进行的科学管理和决策。鉴于此 ,笔者开展了此方面的研究。ＤＰＭＩＳ (养鹿生产管理信息系统 ,ＤｅｅｒＰｒｏｄｕｃｔｉｏｎＭａｕａｇｅｍｅｎｔＩｎｆｏｒｕａｔｏｕｓｙｓｔｅｍ)是信息管理系统的一种 ,是一个适合各种性质、规模养鹿场…  相似文献   

人工草地绵羊系统优化研究Ⅳ人工草地划区轮牧罗姆尼怀孕母羊群体采食量研究     

马宁  耿文诚 《草业科学》2000,17(Z1):43-45

人工草地划区轮牧条件下,牧草生长旺季罗姆尼(Romney)怀孕母羊羊群采食量与怀孕日龄间呈显著正相关,回归方程Y=0.003 2X+0.853 7,r=0.949 5;但霜冻到来后采食量与怀孕日龄间呈显著负相关,回归方程Y=-0.007 3X+1.784 1,r=-0.985 1.  相似文献   

人工草地绵羊系统优化研究Ⅲ几种冬春青绿饲料作物的引种栽培     

耿文诚 《草业科学》2000,17(Z1):40-42

用新西兰引进的波纳、威诺阿、帕斯加、艾品、巴堪特和当地长期种植的芜菁6种冬春青绿饲料作物在云南省种羊场2个气候带种植,对各品种生物量及干鲜比进行比较分析.结果为芜菁生物量及含水量均较引进品种高;在引进品种中,高海拔山区威诺阿表现最佳,中海拔地区艾品较好.  相似文献   

Study on Expression of Bcl-2 and PCNA Genes in Testicular Tissue of Chickens,Quails and Chicken-quail Hybrids     

ZHANG Mei  ZHOU Ling-ling  ZHANG Miao-miao  ZHONG Xin-xian  SHAO Jun  FAN Li-na  GENG Peng-rui  LI Yi-dan  LI Yan  LIAO He-rong 《中国畜牧兽医》2017,44(7):2050-2056

In order to investigate the molecular mechanism of stunted growth testis of chicken (♂) and quail (♀) hybrids. 84 testicular tissue samples of New Roman cocks, male Korean quails and chicken-quail hybrids at different developmental stages were collected,the mRNA expression of Bcl-2 and PCNA genes in testicular tissue of cocks, quails and chicken-quail hybrids at different growth stages were detected using Real-time PCR. The results showed that the Bcl-2 and PCNA genes mRNA expression patterns in testes of chickens and quails at different growth stages were similar.The Bcl-2 gene was fluctuated in a whole, and decreased to the lowest value, then recovered rapidly and remained at a high level. The PCNA gene had a very significant peak value.Compared with the chicken and quail,the expression of Bcl-2 and PCNA genes mRNA in hybrids had no obvious change, which indicated that the pattern of the Bcl-2 gene regulation apoptosis in testis was different from chicken and quail at the hybrid testicular development process, and there was no obvious cell appreciation process.It was suggested that the molecular influencing factors of testicular dysplasia of chicken and quail hybrids were related to the abnormal expression of Bcl-2 and PCNA genes mRNA in testes.The results provided an important reference for the further study of the mechanism of testicular dysplasia between chicken and quail interspecific hybrids.  相似文献