Benefits of using the probiotic Efinol^®L during transportation of cardinal tetra, Paracheirodon axelrodi (Schultz), in the Amazon     

Levy Carvalho Gomes  Richard Philip Brinn  Jaydione Luiz Marcon  Lucelle Araújo Dantas  Franmir Rodrigues Brandão  Janessa Sampaio de Abreu  Paulo Evandro Mendes Lemos  Dawn Michelle McComb  & Bernardo Baldisserotto 《Aquaculture Research》2009,40(2):157-165

The objective of this experiment was to test the probiotic Efinol^®L during transportation of cardinal tetra, Paracheirodon axelrodi (Schultz). For the transportation, fish were distributed in 18 plastic tanks, of which nine contained the Efinol^®L (10 mg L⁻¹; probiotic treatment) and the remaining had no probiotic (control treatment). Transport lasted 24 h and three different boxes of each treatment were sampled at 3, 12 and 24 h. Up to the 12-h sampling period, no significant difference in the survival was observed; on the other hand, survival was higher at the end of the transport (24 h) in the probiotic treatment. No significant difference was found in dissolved oxygen and temperature between treatments. Conductivity, pH and alkalinity increased along the transport, but without a difference between treatments. Ammonia increased in all treatments, although it was significantly lower in the probiotic group at 24 h. Cortisol levels were significantly higher in all transported fish when compared with the basal values. At 24 h, cortisol levels in control fish were significantly higher than those in the probiotic treatment. With the observed results, we are able to conclude that the probiotic Efinol^®L is efficient during cardinal transport, lowering the mortality and helping maintain water quality possibly by lowering metabolic wastes.  相似文献   

Impact of pond management on tambaqui, Colossoma macropomum (Cuvier), production during growth-out phase     

Levy Carvalho Gomes  & Clichenner Rodrigues Silva 《Aquaculture Research》2009,40(7):825-832

This study evaluated the impact of pond management on tambaqui, Colossoma macropomum (Cuvier), rearing during the growout phase. Juvenile tambaqui were stocked in ponds with three different management regimes: (1) natural ponds (Nat); (2) limed ponds (Lim); and (3) limed and bimonthly fertilized ponds (LimFer). The experiment lasted 210 days and the growth parameters were evaluated monthly. Water quality and effluent measurements were performed every 15 days and at the end of the experiment yield parameters were obtained. There was no difference in weight and length among treatments. Stomach contents and zooplankton availability were not influenced by pond management, but the rearing period had an influence on them. Food conversion rate (FCR) was better in fish from the Lim treatment than in fish from the Nat treatment. The pH, hardness and alkalinity values were significantly higher in the Lim and LimFer ponds, where the liming procedure was performed. The effluent analysis showed a more potentially impacting effluent in the LimFer treatment, where phosphorus and orthophosphate concentrations showed values significantly higher than those in Lim and Nat ponds. The results show that the Lim treatment is the best approach, as in this treatment fish achieve market size, better FCR, yield and have a reduced environmental impact.  相似文献   

Effects of photoperiod on survival and growth of the marine pejerrey Odontesthes argentinensis larvae     

Luciano Siqueira Freitas  Ricardo Vieira Rodrigues  Marcelo Hideo Okamoto  Luiz Roberto Louzada  & Luís André Sampaio 《Aquaculture Research》2009,40(2):252-255

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Liver histology and histomorphometry in hybrid sorubim (Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans) reared on intensive fish farming          下载免费PDF全文

Robson Andrade Rodrigues  Klaus Casaro Saturnino  Carlos Eurico Fernandes 《Aquaculture Research》2017,48(9):5083-5093

This study aimed to characterize the liver histology and histomorphometry in sorubim hybrid of different categories (nursery, growth and grow‐out) reared on fish farming. The categories were defined considering body weight (BW): nursery category (n = 5): BW = 37.06 ± 6.00 g (31.6–45.3 g); growth category (n = 5): BW = 310.40 ± 53.80 g (242.1–376.4 g) and grow‐out category (n = 5): BW = 874.28 ± 27.59 g (846.2–913.1 g). Liver fragments were processed to paraffin inclusion, and sections were stained by haematoxylin and eosin (H&E), PAS (Periodic Acid Schiff) and Perl's staining to histology, histomorphometry and density volumetric of liver structures; glycogen analysis and to detect ferric irons (Fe³⁺) respectively. The hepatosomatic index decreased between the categories (P < 0.01). The percentage of PAS‐positive hepatocytes in the nursery category was higher (P < 0.05) in relation to the growth and grow‐out categories. The hepatocytes from all fish were positive to Perl's staining. The density volumetric of liver structures did not differ among categories except to blood vessels were higher (P < 0.01) in the nursery and growth. The area (μm²) and perimeter (μm) of hepatocytes, and the area (μm²), perimeter (μm) and volume (μm³) of the nuclei from grow‐out fish were lower (P < 0.01) than those from the nursery and growth categories. Changes in morphometric characteristics of hepatocytes may result from metabolic changes associated with body growth surubins; therefore, these morphometric characteristics of liver tissue can be used as functional biomarkers for the assessment of fish health and nutrition status.  相似文献   

Anti-SARS-CoV-2 Activity of Rhamnan Sulfate from Monostroma nitidum     

Yuefan Song  Peng He  Andre L. Rodrigues  Payel Datta  Ritesh Tandon  John T. Bates  Michael A. Bierdeman  Chen Chen  Jonathan Dordick  Fuming Zhang  Robert J. Linhardt 《Marine drugs》2021,19(12)

The COVID-19 pandemic is a major human health concern. The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein with a host cell receptor, angiotensin-converting enzyme 2 (ACE2). In addition to ACE2, heparan sulfate (HS) on the surface of host cells also plays a significant role as a co-receptor. Our previous studies demonstrated that sulfated glycans, such as heparin and fucoidans, show anti-COVID-19 activities. In the current study, rhamnan sulfate (RS), a polysaccharide with a rhamnose backbone from a green seaweed, Monostroma nitidum, was evaluated for binding to the S-protein from SARS-CoV-2 and inhibition of viral infectivity in vitro. The structural characteristics of RS were investigated by determining its monosaccharide composition and performing two-dimensional nuclear magnetic resonance. RS inhibition of the interaction of heparin, a highly sulfated HS, with the SARS-CoV-2 spike protein (from wild type and different mutant variants) was studied using surface plasmon resonance (SPR). In competitive binding studies, the IC₅₀ of RS against the S-protein receptor binding domain (RBD) binding to immobilized heparin was 1.6 ng/mL, which is much lower than the IC₅₀ for heparin (~750 ng/mL). RS showed stronger inhibition than heparin on the S-protein RBD or pseudoviral particles binding to immobilized heparin. Finally, in an in vitro cell-based assay, RS showed strong antiviral activities against wild type SARS-CoV-2 and the delta variant.  相似文献   

Improving the estimation of soil water evaporation based on days after wetting     

Élvis da Silva Alves  Lineu Neiva Rodrigues  Fernanda Laurinda Valadares Ferreira 《Journal of Agronomy and Crop Science》2023,209(1):176-187

Soil evaporation constitutes a major pathway of water loss in agriculture. Understanding its dynamics in the face of drying and soil cover is fundamental to improve both simulation models and the sustainability of production systems. Thus, the objective of this study was to estimate soil evaporation as a function of drying and percentage of soil cover. Three experiments were carried out in three different periods. In each of the periods, in parallel and in an adjacent area, an experiment was carried out to evaluate the influence of weighing micro-lysimeter (ML) height on the estimation of direct soil evaporation. The experiments were installed with a completely randomized design. For the experiments that measured evaporation as a function of cover, the treatments consisted of six different percentages of cover of the internal area of the ML (0%, 10%, 25%, 50%, 75% and 100%), with four replicates, using artificial plants to cover the soil. The experiments that estimated evaporation as a function of ML height were conducted with eight repetitions and three different ML heights (100, 200 and 300 mm). It was observed that the accumulated evaporation up to 25 days after wetting was 52% and 53% lower in micro-lysimeters with 100 mm height, compared to those with 200 and 300 mm height, respectively, for all experiments. The new model developed to calculate soil evaporation as a function of the days after wetting and percentage of soil cover showed excellent performance (NSE > 0.95).  相似文献   

A data-driven fuzzy approach to simulate the critical shear stress of mixed cohesive/non-cohesive sediments     

Aline Schäfer Rodrigues Silva  Markus Noack  Dirk Schlabing  Silke Wieprecht 《Journal of Soils and Sediments》2018,18(10):3070-3081

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Autochthonous probiotic as growth promoter and immunomodulator for Astyanax bimaculatus cultured in water recirculation system          下载免费PDF全文

Andressa Vieira de Moraes  Marina de Oliveira Pereira  Klayton Natan Moraes  Jorge P Rodrigues‐Soares  Gabriel Fernandes Alves Jesus  Adolfo Jatobá 《Aquaculture Research》2018,49(8):2808-2814

This work aimed to evaluate the probiotic Lactobacillus spp. in the microbiota of Astyanax bimaculatus reared in a water recirculation system. The experiment was randomized, using 800 postlarvae distributed into eight polyethylene containers, separated into two treatments, in quadruplicate: supplementation with Lactobacillus spp. (Probiotic) and no supplementation (Control). After 90 days of culture, 13 fish per container were anaesthetized. Three of these underwent microbiological evaluation, and haematological analyses were carried out on five. Immunological assessment was performed on another five fish. Zootechnical parameters were evaluated for all animals. In the microbiological evaluation, the probiotic group presented higher counts of lactic acid bacteria, but lower counts of Vibrio spp., Pseudomonas spp., and Staphylococcus spp., when compared with control. After haematological analysis, the supplemented group presented low haematimetric indices of Mean Corpuscular Haemoglobin Concentration and Mean Corpuscular Haemoglobin (MCHC and MCH, respectively) and higher amounts of total leucocytes, thrombocytes and circulating monocytes when compared with control. The immunological profile did not differ between treatments. In the zootechnical performance, probiotic group presented higher productivity, survival and apparent food efficiency when compared with control. Therefore, when Lactobacillus spp. was supplemented in the diet of A. bimaculatus, immunocompetence and the zootechnical performance of animals increased.  相似文献   

A new insight into the influence of habitat on the biochemical properties of three commercial sea cucumber species     

González-Wangüemert  Mercedes  Roggatz  Christina C.  Rodrigues  Maria João  Barreira  Luísa  da Silva  Manuela Moreira  Custódio  Luísa 《International Aquatic Research》2018,10(4):361-373

International Aquatic Research - This work makes a comparative evaluation of the biochemical profile of three sea commercial cucumber species (Holothuria mammata, H. polii and H. tubulosa) caught...  相似文献   

Spray particle drift mitigation using field corn (Zea mays L.) as a drift barrier          下载免费PDF全文

Bruno C Vieira  Thomas R Butts  Andre O Rodrigues  Jeffrey A Golus  Kasey Schroeder  Greg R Kruger 《Pest management science》2018,74(9):2038-2046

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