Vaccination of calves with EspA, a key colonisation factor of Escherichia coli O157:H7, induces antigen-specific humoral responses but does not confer protection against intestinal colonisation     

Dziva F  Vlisidou I  Crepin VF  Wallis TS  Frankel G  Stevens MP 《Veterinary microbiology》2007,123(1-3):254-261

Enterohaemorrhagic Escherichia coli (EHEC) infections in humans are frequently associated with direct or indirect contact with ruminant faeces and may result in haemorrhagic colitis and severe renal and neurological sequelae. Broadly cross-protective vaccines for control of EHEC do not yet exist and the molecular mechanisms that influence bacterial persistence in the intestines of ruminants are incompletely understood. We sought to determine the role in colonisation and protective efficacy of EspA, which forms a filamentous extension of the locus of enterocyte effacement-encoded type III secretion system that injects EHEC proteins into enterocytes. A non-polar deletion of espA severely impaired the ability of E. coli O157:H7 to colonise the intestines of calves. Vaccination of calves with highly purified recombinant EspA induced high-titre antigen-specific IgG1 (also reactive to native EspA) and salivary IgA responses, however these responses did not protect calves against intestinal colonisation by E. coli O157:H7 upon experimental infection.  相似文献   

Splenic immune responses during canine visceral leishmaniasis   总被引：1，自引：0，他引：1  

Strauss-Ayali D  Baneth G  Jaffe CL 《Veterinary research》2007,38(4):547-564

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Mixed Ehrlichia canis, Hepatozoon canis, and presumptive Anaplasma phagocytophilum infection in a dog     

Mylonakis ME  Koutinas AF  Baneth G  Polizopoulou Z  Fytianou A 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(4):249-251

A 5-month-old, female, mongrel dog was admitted to the Clinic of Companion Animal Medicine, Aristotle University of Thessaloniki, Greece, with depression, anorexia, fever, peripheral lymphadenopathy, splenomegaly, oculonasal discharge, nonregenerative anemia, and mild thrombocytopenia. Cytology of Giemsa-stained buffy coat, bone marrow, and lymph node aspiration smears revealed numerous morulae in mononuclear leukocytes and in neutrophils, and Hepatozoon canis gamonts in neutrophils. The dog was seropositive to Ehrlichia canis (immunofluorescence assay [IFA]) and Hepatozoon canis (ELISA) but not to Anaplasma phagocytophilum (IFA). A nested polymerase chain reaction performed on bone marrow aspirates was positive for E canis. This method was not applied for the detection of A phagocytophilum. Treatment with doxycycline and imidocarb dipropionate resulted in both clinical and parasitologic cure. This is the first reported case of a mixed infection with E canis, H canis, and presumptive A phagocytophilum. The findings emphasize the value of cytology in offering a quick and inexpensive diagnosis in mixed tick-borne infections of dogs.  相似文献   

Accidental poisoning of 17 dogs with lasalocid     

Segev G  Baneth G  Levitin B  Shlosberg A  Aroch I 《The Veterinary record》2004,155(6):174-176

Over a period of 10 days, 17 dogs became weak and developed neurological deficits of different degrees of severity. About 12 hours before these clinical signs appeared they had all eaten a particular brand of commercial dog food from a recently opened bag. They were all quadriparetic and hyporeflexic, and some of them also showed additional systemic or neurological signs, including dyspnoea, a high body temperature, tongue laxity, hyperaesthesia and anisochoria. Serum biochemical abnormalities included high activities of creatine kinase, lactate dehydrogenase and aspartate aminotransferase. Analysis of the suspect food revealed high concentrations of the ionophore lasalocid. Fifteen of the dogs were given supportive treatment at home and two were hospitalised. Five of the dogs died, but the others improved gradually and had fully recovered by one to four days after the appearance of the clinical signs.  相似文献   

Canine leishmaniasis: a diagnostic and clinical challenge     

Baneth G  Aroch I 《Veterinary journal (London, England : 1997)》2008,175(1):14-15

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Regression Analysis of Soil Chemical Composition for Two Shrimp Farms in Texas   总被引：1，自引：0，他引：1  

Gad  Ritvo F. Michael  Speed William H.  Neill Joe B.  Dixon Addison L.  Lawrence Tzachi M.  Samocha 《Journal of the World Aquaculture Society》1999,30(1):26-35

Several nutrients, pH, organic matter, and total soluble salts of surface soil from 81 ponds on two Texas shrimp farms were measured. The relationship between shrimp yield and soil characteristics was analyzed statistically. Shrimp yield was negatively correlated with soil concentrations of S, P, NO₃^-, B, Ca, Mg, K, Na, Cu, and total water-soluble salts. Shrimp yield was directly correlated with soil concentrations of Fe, Mn, and organic matter. Stepwise multiple regression analysis indicated that Ca and S concentrations explained 54% of the variation in shrimp yield.  相似文献   

Simultaneous extraction and quantitation of carotenoids, chlorophylls, and tocopherols in Brassica vegetables     

Guzman I  Yousef GG  Brown AF 《Journal of agricultural and food chemistry》2012,60(29):7238-7244

Brassica oleracea vegetables, such as broccoli (B. oleracea L. var. italica) and cauliflower (B. oleracea L. var. botrytis), are known to contain bioactive compounds associated with health, including three classes of photosynthetic lipid-soluble compounds: carotenoids, chlorophylls, and tocopherols. Carotenoids and chlorophylls are photosynthetic pigments. Tocopherols have vitamin E activity. Due to genetic and environmental variables, the amounts present in vegetables are not constant. To aid breeders in the development of Brassica cultivars with higher provitamin A and vitamin E contents and antioxidant activity, a more efficient method was developed to quantitate carotenoids, chlorophylls, and tocopherols in the edible portions of broccoli and cauliflower. The novel UPLC method separated five carotenoids, two chlorophylls, and two tocopherols in a single 30 min run, reducing the run time by half compared to previously published protocols. The objective of the study was to develop a faster, more effective extraction and quantitation methodology to screen large populations of Brassica germplasm, thus aiding breeders in producing superior vegetables with enhanced phytonutrient profiles.  相似文献   

Essential oils and their components as promising approach for gastropod mollusc control: a review     

Radwan  Mohamed A.  Gad  Amira F. 《植物病害和植物保护杂志》2021,128(4):923-949

Journal of Plant Diseases and Protection -  Despite the great benefits of using synthetic molluscicides as mollusc control agents, they can destroy the entire ecosystems,...  相似文献   

BLM heterozygosity and the risk of colorectal cancer     

Gruber SB  Ellis NA  Scott KK  Almog R  Kolachana P  Bonner JD  Kirchhoff T  Tomsho LP  Nafa K  Pierce H  Low M  Satagopan J  Rennert H  Huang H  Greenson JK  Groden J  Rapaport B  Shia J  Johnson S  Gregersen PK  Harris CC  Boyd J  Rennert G  Offit K 《Science (New York, N.Y.)》2002,297(5589):2013

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Vector-borne pathogens in dogs from Costa Rica: First molecular description of Babesia vogeli and Hepatozoon canis infections with a high prevalence of monocytic ehrlichiosis and the manifestations of co-infection     

Alicia Rojas  Diana Rojas  Víctor Montenegro  Ricardo Gutiérrez  Daniel Yasur-Landau  Gad Baneth 《Veterinary parasitology》2014,199(3-4):121-128

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