Seroprevalence to bovine immunodeficiency virus and lack of association with leukocyte counts in Italian dairy cattle     

S. Cavirani  G. Donofrio  D. Chiocco  E. Foni  P. Martelli  G. Allegri  C. S. Cabassi  B. De Iaco  C. F. Flammini 《Preventive veterinary medicine》1998,37(1-4):147-157

We report herein on the first serological detection of antibodies to bovine immunodeficiency virus (BIV) in Italy. According to criteria of a stratified-random sampling of dairy cattle reared in the Parma area (a province in the Po Valley, Northern Italy), sera from 3166 cows belonging to 272 herds were collected. In addition, sera of 138 bulls from eight artificial-insemination (AI) centres were sampled. Seventy-eight cows (2.5%) from 16 herds (5.8%) and seven bulls (5.1%) from two AI centres were positive for BIV-R29 antibodies in the IFA-test. IFA-positive sera assayed by Western blot had reaction to different viral proteins: 81 out of 85 sera showed antibody to p26 (considered the BIV major internal core protein); four sera reacted to other viral proteins but not to p26. Peripheral blood leukocytes of 60 seropositive and 60 seronegative animals, belonging to eight BIV-infected herds, were enumerated to assess any effect of BIV infection on white-blood cells. No significant differences were detected between the two groups. These data indicate that BIV infection is present in Italian dairy cattle – but the role of BIV in inducing disease remains unclear.  相似文献   

Extranodal γδ-T-cell lymphoma in a dog with leishmaniasis     

Valentina Foglia Manzillo  Annalisa Pagano  Roberta Guglielmino  Luigi Gradoni  Brunella Restucci  Gaetano Oliva 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(3):298-301

Abstract: An 8-year-old intact male mongrel dog with alopecia and weight loss was referred to the Veterinary Faculty of Naples. The dog had pale mucous membranes, enlarged prescapular lymph nodes, and splenomegaly. Laboratory abnormalities included anemia, thrombocytopenia, and hyperglobulinemia. Bone marrow aspirate smears contained numerous Leishmania amastigotes and an immunofluorescent antibody titer was strongly positive (1:1280) for leishmaniasis. The dog was treated with a combination of meglumine antimoniate and allopurinol for 60 days and showed clinical improvement. Two months after the end of treatment the dog was again referred because of relapse of leishmaniasis and the presence of a firm subcutaneous mass on the medial right thigh. Based on cytologic examination of fine needle aspirates of the mass, a diagnosis of large-cell lymphoma was made. Flow cytometry of tumor cells revealed γδ-T-cell lymphoma with a CD5+, CD3+, TCRγδ+, CD4−, CD8−, CD45RA+ immunophenotype. Using nested PCR, amastigotes were not detected in the neoplastic tissue. An association between leishmaniasis and hematopoietic tumors has been described rarely. γδ-T cells may be involved in the host response to this parasite, and prolonged antigenic stimulation and chronic immunosuppression (typical of leishmaniasis) play a crucial role in the etiopathogenesis of T-cell lymphoma.  相似文献   

Effect of stocking density and protein/fat ratio of the diet on the growth of Dover sole (Solea solea)     

Giovanni Piccolo  Stefania Marono  Fulvia Bovera  Raffaella Tudisco  Gaetano Caricato  Antonino Nizza 《Aquaculture Research》2008,39(16):1697-1704

Two hundred and fifty soles (30 g initial weight) were randomly stocked in 12 tanks (recirculation system) at two different stocking densities (2.3 and 1.3 kg m^?2) and fed on two different diets (50% and 54% of crude protein and 21% and 18% of total lipid, respectively, for diets A and B) with triplicate tanks for treatment. The trial lasted for 300 days. Fish stocked at low density showed a significantly higher weight gain than the fish reared at 2.3 kg m^?2 density (94.1±7.4 vs. 78.9±8.2 g, P<0.01) with a specific growth rate (SGR) of 0.46±0.11 and 0.43±0.16 (P<0.01) respectively. Feed conversion ratio (FCR) was more favourable for lower density groups (2.50 vs. 2.64, P<0.01). Diet B led to a higher final weight (124.1 vs. 110.2 g, P<0.01) and a better feed utilization (FCR: 2.49 vs. 2.65, P<0.01). Except for the lipid content, which was higher in the low‐density group (2.6% vs. 2.3%, P<0.05), proximate composition of sole's muscle was not influenced by treatments. Dover sole reared at low stocking density showed higher monounsaturated fatty acid (35.27% vs. 34.40%, P<0.01) and lower saturated fatty acid (24.36 vs. 26.13%, P<0.01) contents, and lower atherogenic (0.67 vs. 0.75, P<0.01) and thrombogenic (0.28 vs. 0.32, P<0.01) indexes.  相似文献   

Application of Trichoderma harzianum (T22) and Trichoderma atroviride (P1) as plant growth promoters, and their compatibility with copper oxychloride          下载免费PDF全文

Francesco Vinale　 Gaetano D'' Ambrosio　 Khalid Abadi　 Felice Scala　 Roberta Marra　 David Turrà　 Sheridan L Woo　 Matteo Lorito 《浙江大学学报(农业与生命科学版)》2004,30(4):425-425

Trichoderma strains are used in agriculture because they provide to the plants the following benefits: i) are rhizosphere competence and establish stable rhizosphere microbial communities; ii) control plant disease caused by pathogenic and competitive microflora, by using a variety of mechanisms; iii) improve vegetative growth, root development and yield; iv) make nutrients more available to the plant. In this work we have investigated the ability of T. harzianum T22 and T. atroviride P…  相似文献   

A combined protocol with piroxicam,chemotherapy, and whole pelvic irradiation with simultaneous boost volumetric modulated arc radiotherapy for muscle-invasive canine urinary transitional cell carcinoma: First clinical experience     

Luca MALFASSI  Francesca FIDANZIO  Massimo SALA  Silvia MARCARINI  Giovanni MAZZA  Nancy CARRARA  Simone PAVESI  Giacomo GNUDI  Gaetano URSO  Mario DOLERA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):695

The aims of this pilot study were to evaluate the feasibility and efficacy of high-dose hypofractionated volumetric modulated arc radiotherapy (VMAT) applied to whole pelvic region radiotherapy (WPRT) with multilevel simultaneous integrated boost (MLSIB) combined with piroxicam and chemotherapy for the treatment of canine transitional cell carcinoma (TCC) of the lower urinary tract with muscle invasion TCC. Twelve dogs were enrolled, according to stage, in two groups: group 1, TCC confined to the urinary tract; group 2, TCC with metastasis. The planning target volume dose was tailored from 36 to 42 Gy in 6 fractions. All dogs were prescribed piroxicam and radiosensitizing carboplatin, and six received chemotherapy after radiotherapy. Serial follow-ups with computed tomography and magnetic resonance imaging were performed. Disease control and toxicity effects were evaluated according to the Response Evaluation Criteria in Solid Tumors and Veterinary Radiation Therapy Oncology Group criteria. The treatment was well tolerated, and no high-grade side effects were reported. The median overall survival times for groups 1 and 2 were 1,230 and 150 days, respectively. A considerable percentage of patients in group1 (50%) were still alive at the time of writing this paper, and a longer follow-up could enable a more accurate survival analysis. This preliminary analysis shows that VMAT applied to the WPRT with MLSIB is an effective and safe option for dogs with lower urinary TCC, although the presence of metastases worsens the prognosis.  相似文献   

Performance of durum wheat landraces in a Mediterranean environment (eastern Sicily)     

Luciano Pecetti  Gaetano Boggini  John Gorham 《Euphytica》1994,80(3):191-199

Summary The evaluation aimed at identifying landrace genotypes adapted to the rather unfavourable growing conditions of durum wheat in Sicily, to be used as parental material in a breeding programme. The trial was carried out in three seasons experiencing varying climatic conditions, and included 75 landraces, 25 of which were selected under severe drought in Syria. Wide differences were observed for most traits among genotypes and seasons of evaluation. Yields of the best performing entries identified in each season never significantly differed from that of the best check variety. The top-yielding landraces were consistently better than the remaining entries for the three yield components, viz. number of spikes per plant, number of kernels per spike and mean kernel weight. In the driest season they were also significantly earlier in heading, confirming the importance of earliness under drought. An analytical breeding approach relying on an array of morpho-physiological traits as selection criteria did not seem appropriate for the given environment, as the variable stress level enhanced the importance of specific traits under specific situations. The genotypic response was largely season-specific. Nonetheless, five landraces were in the best group in all seasons. The selection made in Syria also appeared somewhat effective in Sicily, particularly in the less favourable seasons.  相似文献   

Lichens Used as Monitors of Atmospheric Pollution Around Agadir (Southwestern Morocco)—A Case Study Predating Lead-Free Gasoline     

Fabrice Monna  Lhoussaine Bouchaou  Claire Rambeau  Rémi Losno  Olivier Bruguier  Gaetano Dongarrà  Stuart Black  Carmela Chateau 《Water, air, and soil pollution》2012,223(3):1263-1274

More than 30 epiphytic lichens, collected in Agadir (Morroco) and along a 150-km transect from the Atlantic Ocean eastward, were analyzed for their metal content and lead isotopic composition. This dataset was used to evaluate atmospheric metal contamination and the impact of the city on the surrounding area. The concentrations of Cu, Pb, and Zn (average ± 1 SD) were 20.9 ± 15.2 μg g⁻¹, 13.8 ± 9.0 μg g⁻¹, and 56.6 ± 26.6 μg g⁻¹, respectively, with the highest values observed in lichens collected within the urban area. The ²⁰⁶Pb/²⁰⁷Pb and ²⁰⁸Pb/²⁰⁷Pb ratios in the lichens varied from 1.146 to 1.186 and from 2.423 to 2.460, respectively. Alkyllead-gasoline sold in Morocco by the major petrol companies gave isotopic ratios of ²⁰⁶Pb/²⁰⁷Pb = 1.076–1.081 and ²⁰⁸Pb/²⁰⁷Pb = 2.348–2.360. These new, homogeneous values for gasoline-derived lead improve and update the scarce isotopic database of potential lead sources in Morocco, and may be of great value to future environmental surveys on the presence of lead in natural reservoirs, where it persists over time (e.g., soils and sediments). The interest of normalizing metal concentrations in lichens to concentrations of a lithogenic element is demonstrated by the consistency of the results thus obtained with lead isotopic ratios. Leaded gasoline contributed less than 50% of the total amount of lead accumulated in lichens, even in areas subject to high vehicular traffic. This strongly suggests that the recent banishment of leaded gasoline in Morocco will not trigger a drastic improvement in air quality, at least in Agadir.  相似文献   

Impact of new measured Mediterranean mineralization rates on the fate of simulated aquaculture wastes     

Patrizia De Gaetano  Paolo Vassallo  Marco Bartoli  Daniele Nizzoli  Andrea M. Doglioli  Marcello G. Magaldi  Mauro Fabiano 《Aquaculture Research》2011,42(9):1359-1370

In order to provide values of key parameters in aquaculture waste degradation modelling specifically for the Mediterranean, sampling campaigns were carried out in 2006. Accurate measurements of particulate carbon input and benthic respiration rates were performed using sediment traps and intact core incubations. The in situ measurements, compared with data from Atlantic salmon production, showed lower carbon flux and oxygen consumption, while a greater degradation capability was observed. Moreover, a temperature dependence of the benthic parameters was highlighted. Successively, the model Finite Organic Accumulation Module was used for an accurate comparison between different parameterizations. Finite Organic Accumulation Module simulates the organic carbon degradation and the net carbon accumulation on the sediment, yielding a benthic state index. In comparison with previous results, there was a decrease in benthic impacts due to minor inputs of carbon and higher mineralization rates. Moreover, a seasonal variation could be observed in the organic carbon concentration. Nevertheless, the new results remain consistent with the old ones on two points: (a) the negligible benthic impact of faeces with respect to uneaten feed and (b) the dependence of that impact on the different feed release conditions.  相似文献   

Preventing species invasion: A role for integrative taxonomy?          下载免费PDF全文

Maria V. MAZZAMUTO  Andrea GALIMBERTI  Giacomo CREMONESI  Benoît PISANU  Jean‐Louis CHAPUIS  Jan STUYCK  Giovanni AMORI  Haijun SU  Gaetano ALOISE  Damiano G. PREATONI  Lucas A. WAUTERS  Maurizio CASIRAGHI  Adriano MARTINOLI 《Integrative zoology》2016,11(3):214-228

Integrative taxonomy, a multi‐disciplinary approach adding modern techniques to traditional morphology‐based methods (e.g. molecular and morphological criteria), can play an important role in bioinvasion research to identify introduced taxa, discover pathways of introduction and inform authorities to control and prevent future introductions. The present study is the first on introduced populations of Callosciurus, Asiatic tree squirrels, known as potentially invasive species in Europe (Italy, Belgium and France). We combined molecular (mitochondrial DNA markers: CoxI, D‐loop) and morphometric analysis on skulls, comparing them to the widest morphological and molecular datasets ever assembled for Callosciurus. Squirrels collected in Italy and Belgium share the same haplotypes and skull characteristics, but are conspicuously different from the French population in Antibes. Genetic data revealed close similarity between French squirrels and Pallas's squirrels, Callosciurus erythraeus, from Taiwan, China. Italian and Belgian squirrels formed an independent taxonomic lineage in genetic analyses, whose taxonomic rank needs further investigation. The morphological and morphometric characteristics of these 2 populations are, however, similar to known specimens assigned to Callosciurus erythraeus. These results may indicate a common origin for the populations found in Belgium and Italy. In contrast, French specimens suggest an independent introduction event of squirrels originating from Asia.  相似文献   

Association Between Neospora Caninum Antibodies and Blue Tongue Vaccination in Dairy Cows     

Cavirani  S.  Cabassi  C. S.  Taddei  S.  Donofrio  G.  Bottarelli  E. 《Veterinary research communications》2015,29(2):233-236

Veterinary Research Communications -  相似文献