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41.
Galina P. Simeonova Svetozar Z. Krastev Radostin S. Simeonov 《Veterinary research communications》2016,40(3-4):107-115
Background
The pathogenic mechanism of equine recurrent uveitis (ERU) is still poorly defined and many variations between experimental animal models and spontaneous disease exist.Objectives
The aim of our study was to investigate if Th17 cell-mediated response plays role in the pathogenesis of the used experimental model in horses and to reveal its pathological findings.Methods
Experimental uveitis was induced in 6 healthy horses. The concentrations of retinal autoantigen CRALBP and IL-17 were measured using ELISA in aqueous humor and vitreous body of the 12 inflamed eyes as well as in 12 control non-inflamed eyes taken from 6 horses in slaughter house. After centrifugation of the two eye media, smears were prepared and cytological investigation was performed. Tissue specimens were taken from all eye globes and were submitted to histopathological investigation.Results
CRALBP and IL-17 concentrations were significantly elevated in eye media of horses with experimental uveitis in comparison with controls. Cytological and histopathological findings corresponded to the changes characteristic of chronic immune-mediated inflammation with mononuclear cell infiltration of uvea, choroid, retina, and eye media as well as severe retinal destruction.Conclusions
Our study demonstrated the involvement of the retinal autoantigen CRALBP as well as IL-17 in the pathogenesis of experimental uveitis in horses. These findings suggests that this experimental uveitis in horses may serve as a suitable animal model for investigation of IL-17- mediated immune response during spontaneous autoimmune uveitis in horses as well as in humans.42.
Hayes AM Gregory SP Murphy S McConnell JF Patterson-Kane JC 《The Journal of small animal practice》2007,48(5):288-291
An 11-year-old, female, spayed cocker spaniel was presented with dysphonia caused by a solitary laryngeal mass. Excisional biopsy was performed, and a diagnosis of plasmacytoma was made on the basis of histological examination. Further investigations showed no signs of systemic involvement. Coarse fractionated radiation therapy failed to control the tumour. Therapy was successfully instituted with a conventional combination chemotherapy protocol over a period of 14 months. The dog remains disease free 30 months after diagnosis. Most solitary, extramedullary plasmacytomas in dogs arise in the gastrointestinal tract, with fewer reports in other sites. The larynx is an uncommon sight of involvement in any species, and to the authors' knowledge, this is the first report of this tumour type in the canine larynx. In contrast to the therapeutic benefits reported in humans, the combination of surgery and radiation therapy was unsuccessful in this case, although sustained remission was gained following chemotherapy. 相似文献
43.
44.
Jilli Crosby BVetMed DVM Karen Humm MA VetMB MSc DACVECC DECVECC Simon D. Cook BSc BVSc MVetMed DACVECC DECVECC 《Journal of Veterinary Emergency and Critical Care》2023,33(1):91-97
Objective
To describe the use of small-bore wire-guided catheters in the management of peritoneal effusion in cats and dogs and to detail any associated adverse events.Design
Retrospective study.Setting
University teaching hospitalAnimals
Forty-five client-owned animals that had peritoneal catheters placed for management of peritoneal effusion between July 2010 and June 2021.Interventions
None.Measurements and Main Results
Forty-five cases were included (25 dogs and 20 cats). Twenty-eight animals had the catheter placed to aid management of a uroabdomen, 8 of which recovered without surgical management, 11 had the catheter placed to allow autotransfusion of hemoabdomen, 3 had peritonitis, and 3 had ascites secondary to cardiac disease. Twenty-seven cases (15 dogs and 12 cats) received sedation (n = 24) or local anesthesia alone (n = 3) to facilitate catheter placement, and 6 cases had the catheter placed while under general anesthesia. Median length of catheter persistence was 24 hours (range: 2–144 h). The most common adverse events reported were impaired drainage (n = 7) and leakage at the insertion site (n = 4).Conclusions
Peritoneal catheters can be inserted percutaneously for management of peritoneal effusion. Indications include stabilization and conservative management of uroabdomen, and autotransfusion. They can often be placed with minimal or no sedation and adverse events appear infrequent in occurrence. 相似文献45.
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48.
W.H. Terjung H-Y. Ji J.T. Hayes P.A. O&#x;Rourke P.E. Todhunter 《Agricultural Water Management》1983,6(1):43-64
A basic parametric crop water use model (WATER) that employes climatic and environmental data to calculate temporal and spatial water consumption for a variety of major corps was applied specifically for grain corn to the region of China and Korea to investigate the evapotranspiration (ET) demand on grain corn and the associated irrigation water applications necessary for optimal crop production. A network of 241 stations provided the seasonal climatic input. The climatic input consisted of data averaged over approximately a 20 year period. Among the results, highest ET under full irrigation (first harvest) occurred in the northwestern inland sections of China, whereas least ET was found for the southeast. Under rainfed conditions, the relationship became nearly inverse. In order to achieve optimum crop yields, about 1000 mm of irrigation water was needed in the northwest, contrasted with none required in the south and east of China. A sensitivity analysis was applied to determine the degree of error introduced by faulty or uncertain environmental input data. 相似文献
49.
Natalia V. Krylova Anna O. Kravchenko Olga V. Iunikhina Anastasia B. Pott Galina N. Likhatskaya Aleksandra V. Volod&#x;ko Tatyana S. Zaporozhets Mikhail Y. Shchelkanov Irina M. Yermak 《Marine drugs》2022,20(1)
The structural diversity and unique physicochemical properties of sulphated polysaccharides of red algae carrageenans (CRGs), to a great extent, determine the wide range of their antiviral properties. This work aimed to compare the antiviral activities of different structural types of CRGs: against herpes simplex virus type 1 (HSV-1) and enterovirus (ECHO-1). We found that CRGs significantly increased the resistance of Vero cells to virus infection (preventive effect), directly affected virus particles (virucidal effect), inhibited the attachment and penetration of virus to cells, and were more effective against HSV-1. CRG1 showed the highest virucidal effect on HSV-1 particles with a selective index (SI) of 100. CRG2 exhibited the highest antiviral activity by inhibiting HSV-1 and ECHO-1 plaque formation, with a SI of 110 and 59, respectively, when it was added before virus infection. CRG2 also significantly reduced the attachment of HSV-1 and ECHO-1 to cells compared to other CRGs. It was shown by molecular docking that tetrasaccharides—CRGs are able to bind with the HSV-1 surface glycoprotein, gD, to prevent virus–cell interactions. The revealed differences in the effect of CRGs on different stages of the lifecycle of the viruses are apparently related to the structural features of the investigated compounds. 相似文献
50.
为了研究甘草总黄酮对高脂饲料造成的罗非鱼(Oreochromis niloticus)脂肪肝损伤是否具有保护作用,随机将180尾健康无伤罗非鱼分成6组,即对照组、高脂饲料模型组和4个不同浓度甘草总黄酮组(0.05、0.10、0.50、1.00 g·kg-1甘草总黄酮),每组30尾。饲喂90 d后,采集罗非鱼各实验组肝,测定肝匀浆中超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)活性、丙二醛(MDA)含量、甘油三酯(TG)含量、总胆固醇(TC)含量、总抗氧化能力(T-AOC)水平,Western blotting法检测肝中核转录因子-κB P65(NF-κB P65)和核转录因子-κB C-Rel (NF-κB C-Rel)蛋白表达水平,RT-PCR法测定肝中微粒体甘油三酸酯转移蛋白(MTTP)和载脂蛋白B100(ApoB100)基因表达情况。结果表明:与对照组相比,高脂饲料模型组罗非鱼肝匀浆中TG、TC、MDA含量极显著(P<0.01)升高,GSH活性、SOD活性、T-AOC水平均极显著(P<0.01)降低;MTTP和ApoB100 mRNA表达水平降低(P<0.01或P<0.05);Western blotting结果显示,NF-κB P 65和C-Rel蛋白表达水平升高。饲料中加入甘草总黄酮后,与高脂饲料模型组相比,罗非鱼肝中GSH活性、SOD活性、T-AOC水平不同程度提高,TC、TG、MDA含量和NF-κB P65、C-Rel蛋白表达水平不同程度降低,MTTP和ApoB100的mRNA表达水平升高,以0.50 g·kg-1甘草总黄酮组效果较好。说明甘草总黄酮对于罗非鱼脂肪肝损伤具有一定的保护作用,可以缓解高脂饲料造成的脂肪肝损伤。 相似文献