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101.
Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD(4+)CD8(low) and CD(4-)CD8(low) alphabeta T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of gammadelta TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. 相似文献
102.
Totté P Rodrigues V Yaya A Hamadou B Cisse O Diallo M Niang M Thiaucourt F Dedieu L 《Veterinary research》2008,39(1):8
A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+ T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP. 相似文献
103.
Vineyard evaluation of stilbenoid‐rich grape cane extracts against downy mildew: a large‐scale study
104.
Mecoprop was irradiated under various conditions of pH, oxygenation and wavelengths in order to study the reactions involved in the phototransformation. Four main photoproducts were identified: 2‐(4‐hydroxy‐2‐methylphenoxy)propionic acid ( I ), o‐cresol ( II ), 2‐(5‐chloro‐2‐hydroxy‐3‐methylphenyl)propionic acid ( III ) and 4‐chloro‐o‐cresol ( IV ). When the anionic form of mecoprop was irradiated between 254 nm and 310 nm (UV‐C or UV‐B), I was the main photoproduct. At 254 nm its formation initially accounted for more than 80% of the transformation. It has not previously been reported in the literature. The reaction results from a heterolytic photohydrolysis. Product II accounted for only a low percentage of the transformation. The stoichiometry was different with the molecular form: the main photoproduct, III , resulted from a rearrangement after a homolytic scission. Products I, II and IV were also formed as minor photoproducts. Some other minor photoproducts were also identified. In contrast, IV was the main photoproduct under sunlight irradiation or when solutions were irradiated in near‐UV light (UV‐A). This wavelength effect is attributed to the involvement of an induced phototransformation; IV is also the main photoproduct when the phototransformation is induced by Fe( III ) perchlorate or nitrite ions. In usual environmental conditions the excitation of the molecular form is negligible and the phototransformation is mainly due to induced photoreactions. © 2000 Society of Chemical Industry 相似文献
105.
Brice Marolleau Aurélien Petiteau Marie-Noëlle Bellanger Mélanie Sannier Nadège Le Pocreau Laetitia Porcher Sophie Paillard Fabrice Foucher Tatiana Thouroude Laurana Serres-Giardi Gabriela Aguileta Annie Chastellier Caroline Bonneau Bruno Le Cam Vanessa Soufflet-Freslon Laurence Hibrand-Saint Oyant 《Plant pathology》2020,69(6):1093-1107
The haploid ascomycete Diplocarpon rosae is the causal agent of black spot disease on roses, a widespread and devastating disease in the outdoor landscape. In this study, we established a Eurasian collection of 77 monoconidial strains of D. rosae: 50 strains collected on cultivated roses in Europe and Asia, and 27 strains on wild roses in Kazakhstan. To provide tools to describe its biology and to study its genetic diversity, we sequenced two strains of D. rosae using Illumina paired-end technology. The genome sizes of these two strains were estimated at 31.1 and 35.2 Mb, which are two times smaller than the genome size of the unique strain previously published. A BUSCO analysis confirmed a genome duplication of the strain previously sequenced and partial gene duplication of strains analysed in this study. Using the two genome sequences, 27 polymorphic microsatellite markers were identified. Polymorphism analysis of the 77 strains revealed a strong genetic differentiation between strains from cultivated and wild roses, and a lower diversity within the fungal population from cultivated roses compared to the population from wild roses. Pathogenicity of 10 strains was evaluated on 9 rose cultivars inoculated in the greenhouse. Disease scoring allowed the classification of strains into three groups and the characterization of resistance of rose cultivars. Good correlation observed between resistance scoring in greenhouse conditions and in the field indicates that pathogenicity assays in controlled conditions could be very useful in the near future to rapidly characterize the resistance of new rose varieties to black spot disease. 相似文献
106.
107.
Trevor B. Enberg DVM Laurence D. Braun DVM DACVECC Alan B. Kuzma DVM DACVS 《Journal of Veterinary Emergency and Critical Care》2006,16(1):34-43
Objective: Five canine cases of gastrointestinal (GI) perforation and septic peritonitis associated with the routine use of meloxicam are reviewed. Series summary: Selective cyclooxygenase‐2 (COX‐2) non‐steroidal anti‐inflammatory drugs (NSAIDs) are being used more extensively and routinely for acute and chronic pain as well as for perioperative management of pain. These medications are safe and effective but can be associated with known GI and renal side effects. The patients in this case series had no significant concurrent illness, were not on any concurrent medication known to potentiate the ulcerogenic effects of NSAIDs, and in most cases did not display clinical signs that were apparent to the owners until the time of perforation. New or unique information provided: Despite the preferential selectivity for COX‐2, newer NSAIDs still carry the risk of GI performation. The incidence of GI perforation may be increased with inappropriate dosing regimens, with use of non‐veterinary products and in animals that are at high risk for toxicity. Early signs of toxicity may include alteration in appetite, and subtle signs of nausea during treatment. Warning owners to monitor their pet for vomiting, melena, and hematemesis may not be sufficient to avoid the potential disastrous consequences of GI ulceration. 相似文献
108.
109.
Antony F. Machin Heather Rogers Alan J. Cross Michael P. Quick Laurence C. Howell Norman F. Janes 《Pest management science》1975,6(5):461-473
The phosphorothionate insecticide diazinon was incubated with liver microsomes from the sheep, cow, pig, guinea-pig, rat, turkey, chicken and duck. Metabolism by liver slices of most of these species was also examined. Hydroxydiazinon, isohydroxydiazinon, dehydrodiazinon, their oxons and diazoxon were identified and determined quantitatively or semi-quantitatively. An eighth metabolite was tentatively identified as the 6-aldehyde analogue of diazinon. Yields and rates of production of these metabolites varied greatly between species. Production of oxons was not generally correlated with susceptibility to diazinon poisoning, although it was lowest in the least susceptible animal, the sheep. The degradation of oxons by liver slices was too slow to explain the low toxicity of diazinon to the mammals. The relative importance of hepatic and extrahepatic metabolism in determining toxicity to vertebrates is discussed. 相似文献
110.