Canine influenza virus     

Smith KC  Daly JM  Blunden AS  Laurence CJ 《The Veterinary record》2005,157(19):599

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Therapeutic effects of recombinant feline interferon-omega on feline leukemia virus (FeLV)-infected and FeLV/feline immunodeficiency virus (FIV)-coinfected symptomatic cats   总被引：1，自引：0，他引：1  

de Mari K  Maynard L  Sanquer A  Lebreux B  Eun HM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(4):477-482

The clinical efficacy of a recombinant feline interferon, rFeIFN-omega, was evaluated for the treatment of cats presented with clinical signs associated with feline leukemia virus (FeLV) infection and FeLV/feline immunodeficiency virus (FIV) coinfection in the field. In this multicentric, double-blind, placebo-controlled trial, 81 cats meeting the inclusion criteria were randomly placed into 2 groups and treated subcutaneously with rFelFN-omega (1 million [M]U/kg per day) or placebo once daily for 5 consecutive days in 3 series (day 0, 14, 60). The cats were monitored for up to 1 year for clinical signs and mortality. During the initial 4-month period, interferon (IFN)-treated cats (n = 39) had significantly reduced clinical scores compared with placebo (n = 42), with all cats having received concomitant supportive therapies. Compared with the control, the IFN-treated group showed significantly lower rates of mortality: 39% versus 59% (1.7-fold higher risk of death for controls) at the 9-month time point and 47% versus 59% (1.4-fold higher risk of death for controls) at the 12-month time point. The IFN treatment was associated with minor but consistent improvement in abnormal hematologic parameters (red blood cell count, packed cell volume, and white blood cell count), apparently underlying the positive effects of IFN on clinical parameters. These data demonstrate that rFeIFN-omega initially has statistically significant therapeutic effects on clinical signs and later on survival of cats with clinical signs associated with FeLV infection and FeLV/FIV coinfection.  相似文献   

Isolation of Babesia divergens from carrier cattle blood using in vitro culture     

Malandrin L  L'Hostis M  Chauvin A 《Veterinary research》2004,35(1):131-139

Babesia divergens, the main causative agent of bovine babesiosis in Western Europe, was isolated from naturally infected cattle. Ninety-six blood samples were examined by means of an in vitro culture technique in sheep erythrocytes: 19 of them were collected from animals in the acute phase of the disease with visible parasitemia on blood smears, while the 77 remaining animals showed no microscopically detectable parasites. B. divergens was cultured from the 19 first blood samples as well as from 31 samples collected from asymptomatic animals. The time period before parasites could be detected in the culture varied in the latter samples from 6 to 20 days. The effects of sampling condition (anticoagulant used) and storage length were tested. A good correlation was obtained between immunofluorescent antibody test and culture, with identical results (positive or negative) for 89.6% of the samples collected from asymptomatic animals. The sensitivity of the in vitro culture method was determined and was about 10 parasites/mL of whole blood from three independent experiments performed with three different isolates, confirming its suitability to detect and culture diverse B. divergens isolates from carrier cattle. The parasites could indeed be isolated 9 months after the acute babesiosis phase in the blood of naturally infected animals. The 50 isolates collected in this study were successfully subcultured, cryopreserved and resuscitated using the same culture medium. The in vitro isolation of B. divergens from asymptomatic carrier cattle was achieved and will allow the analysis of parasite diversity within cattle herds.  相似文献   

Development of an indirect enzyme-linked immunosorbent assay for detecting equine serum antibodies to the lipopolysaccharide of Salmonella abortusequi     

Gall D  Nielsen K  Bermudez RM  Muñoz del Real MC  Halbert G  Groulx R  Moreno F  Chow EY  Checkley SL 《Research in veterinary science》2006,81(2):215-217

An indirect enzyme-linked immunosorbent assay (IELISA) was developed for the detection of equine serum antibodies to lipopolysaccharide of Salmonella enterica subsp. enterica serovar Abortusequi (LPS), a causative organism of Equine Paratyphoid. The data presented demonstrates that horses immunized with S. abortusequi LPS developed antibodies detectable by the IELISA. By comparison, the tube agglutination test (TAT) did not detect antibody to S. abortusequi LPS as consistently as the IELISA. The data suggests that the IELISA may be a more suitable test for the detection of serum antibodies to S. abortusequi than the TAT.  相似文献   

Anaemia, iron status and vitamin A deficiency among adolescent refugees in Kenya and Nepal     

Woodruff BA  Blanck HM  Slutsker L  Cookson ST  Larson MK  Duffield A  Bhatia R 《Public health nutrition》2006,9(1):26-34

OBJECTIVE: To investigate the prevalence of anaemia (haemoglobin<11.0 to 13.0 g dl(-1) depending on age and sex group), iron deficiency (transferrin receptor concentration>8.3 microg ml(-1)) and vitamin A deficiency (serum retinol <0.7 micromol l(-1)) in adolescent refugees. DESIGN: Cross-sectional surveys. SETTING: Kakuma refugee camp in Kenya and seven refugee camps in Nepal. SUBJECTS: Adolescent refugee residents in these camps. RESULTS: Anaemia was present in 46% (95% confidence interval (CI): 42-51) of adolescents in Kenya and in 24% (95% CI: 20-28) of adolescents in Nepal. The sensitivity of palmar pallor in detecting anaemia was 21%. In addition, 43% (95% CI: 36-50) and 53% (95% CI: 46-61) of adolescents in Kenya and Nepal, respectively, had iron deficiency. In both surveys, anaemia occurred more commonly among adolescents with iron deficiency. Vitamin A deficiency was found in 15% (95% CI: 10-20) of adolescents in Kenya and 30% (95% CI: 24-37) of adolescents in Nepal. Night blindness was not more common in adolescents with vitamin A deficiency than in those without vitamin A deficiency. In Kenya, one of the seven adolescents with Bitot's spots had vitamin A deficiency. CONCLUSIONS: Anaemia, iron deficiency and vitamin A deficiency are common among adolescents in refugee populations. Such adolescents need to increase intakes of these nutrients; however, the lack of routine access makes programmes targeting adolescents difficult. Adolescent refugees should be considered for assessment along with other at-risk groups in displaced populations.  相似文献   

Retrospective. Oscar Miller (1925-2012)     

McKnight S  Beyer A  Gall J 《Science (New York, N.Y.)》2012,335(6075):1457

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Ultrasonography of the Collateral Ligaments of the Distal Interphalangeal Joint in Horses: Technique and Reference Images     

Laurence Evrard  DVM  Géraldine Bolen  Nathalie MaquetValeria Busoni  DVM  PhD  Dip. ECVDI 《Journal of Equine Veterinary Science》2012

This study describes ultrasound (US) technique and reference images of the equine distal interphalangeal joint collateral ligaments (CLs), and evaluates the portion of the CLs assessable by US in a series of normal forelimbs. Transverse and longitudinal US images were obtained on five healthy horses and on 25 equine cadaver forelimbs. On six limbs, a needle was placed under US-guidance at the distal limit of visualization of each CL, and the portion of CL visible at US was evaluated on computed tomographic (CT) images. The normal CLs appear as oval structures located abaxial to the fossae of the middle phalanx in the transverse sections, obtained at the level of the coronary band. A centrodorsal hypoechoic image appears with increasing proximodistal probe inclination, demonstrating different fiber orientations within the ligament. Two main fascicles, a deep and a superficial, distally divergent, are visible on longitudinal images obtained in the central part of the ligament. The proportion of CL visible at US examination was more than 50% of the total ligament length in nine of the 12 CLs assessed by CT. Awareness of the estimated portion of distal interphalangeal joint CLs visible at US and detailed knowledge of the US technique and CLs morphology are essential to efficiently use US examination on clinical cases.  相似文献   

Modeling the effect of initial planting density on within tree variation of stiffness in loblolly pine     

Finto Antony  Laurence R. Schimleck  Lewis Jordan  Richard F. Daniels  Alex Clark III 《Annals of Forest Science》2012,69(5):641-650

Context

Modulus of elasticity (MOE) is an important mechanical property determining the end-use and value of loblolly pine (Pinus taeda L.) lumber.

Aim

In this study, a model was developed to predict the within tree variation of MOE, from pith-to-bark and stump-to-tip, using data collected from a 21-year-old unthinned stand where trees were planted under seven initial stand density levels (746?C2,243 trees/hectare).

Methods

The study was laid out in a randomized complete block design, with seven levels of initial planting density, replicated three times. Seven trees were destructively sampled from each plot, and bolts were cut from each tree at heights of 2.4, 7.3, and 12.2?m. Static bending samples (of dimension 2.5?×?2.5?×?40.6?cm) representing pith-to-bark variation were cut from each bolt and MOE measured. A three-parameter logistic function was used to model the pith-to-bark variation in stiffness with distance from pith as an explanatory variable.

Results

Based on the final fitted model, it was found that the asymptotic parameter (maximum outerwood MOE?=?13.48?GPa) was not influenced by sampling height, initial planting density, and stem slenderness. However, the inflection and scale parameters were significantly influenced by these variables.

Conclusions

In summary, we found that initial planting density had a significant influence on the amount of corewood produced with higher initial planting densities producing a lower proportion of corewood as indicated by a linear decrease in inflection point with an increase in planting density.  相似文献   

Evaluation of five serological tests for the diagnosis of porcine brucellosis in French Polynesia     

Anne Praud  Olivier Gimenez  Gina Zanella  Nathalie Pozzi  Valérie Antras  Laurence Meyer  Bruno Garin-Bastuji 《Tropical animal health and production》2013,45(4):931-933

Porcine brucellosis due to Brucella suis biovar 1 raises important issues for pig breeders in French Polynesia. In this region, the disease is enzootic, spreads silently and engenders economic losses in infected farms as well as sporadic human cases. While serological tests are essential in surveillance and control programmes of animal diseases, to date none of the available tests have been shown to be reliable enough to be used as a gold standard in routine individual diagnosis of porcine brucellosis. Few studies about the estimation of the sensitivity and the specificity of porcine brucellosis screening tests have been published, none of them dealing with French Polynesia. The studied population included 1,595 pigs from French Polynesia. Five tests were evaluated: Rose Bengal test, fluorescence polarisation assay, indirect ELISA, and two competitive ELISAs (C-ELISA). The sensitivity and the specificity of each test were estimated. C-ELISA₂ was the most sensitive test (Se C-ELISA₂?=?0.954 [0.889; 0.992] 95 % credibility interval (CrI)) while both C-ELISA and Rose Bengal test (RBT) were the most specific ones (Sp C-ELISA₁?=?0.856 [0.806; 0.915] 95 % CrI; Sp C-ELISA₂?=?0.849 [0.817; 0.879] 95 % CrI; Sp RBT?=?0.853 [0.812; 0.898] 95 % CrI).  相似文献   

A description and evaluation of the physical and financial characteristics of dairy farms operating on soil types classified as poorly drained and associated with high rainfall     

Liam Hanrahan  Pat Tuohy  Noirin McHugh  James O'Loughlin  Brian Moran  Pat Dillon  James Breen  Michael Wallace  Laurence Shalloo 《Grass and Forage Science》2019,74(3):548-558

The physical and financial performance data used for analysis were obtained over a 6‐year period (2010–2015), from two different databases including a nationally representative data set of dairy farmers and a detailed data set of seven individual farms. Initial analysis indicated significant variations across soil type and region, across a wide range of physical and financial parameters. Further detailed analysis was completed using a group of seven farms participating in a Heavy Soils Programme (HSP), which were compared to different cohorts of the nationally representative database (National Farm Survey [NFS]), ranked on net profit per ha. The HSP farms utilized larger quantities of grass DM per ha per year than the median of the NFS farms, at similar grazing season lengths, but were using lower levels of purchased feed. Economic analysis indicated the HSP farmers achieved significantly lower net profits per hectare to the NFS median group but significantly higher net profits per kg of fat and protein. The HSP farms also achieved significantly greater overall net farm income per year (€83,788), when compared to the median nationally (€67,898), over the 6‐year period (excluding owned land and labour). The mean return on assets ascertained by the HSP farmers was also significantly greater, at 5.75% per year compared to 3.49% achieved by the median of NFS farms. In conclusion, this study has indicated that efficient dairy businesses operating on poorly drained soils can be as profitable as those across all ranges of soil types.  相似文献