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41.
Conclusive evidence is presented for the existence of energetic ( approximately 535,0000 to 150,000 electron volts), heavy (>-12 atomic mass units), singly charged cometary ions within approximately 1.5 x 10(6) kilometers of comet Giacobini-Zinner. The observations were made with the University of Maryland/Max-Planck-Institut ultralow-energy charge analyzer on, the International Cometary Explorer spacecraft. The most direct evidence for establishing the mass of these ions was obtained from an analysis of the energy signals in one of the solid-state detectors; it is significant at the three-sigma level. Maximum fluxes were recorded approximately 1 hour before and approximately 1 hour after closest approach to the cometary nucleus. Transformation of the particle angular distributions observed at approximately 50,000 kilometers radial distance from the comet during the inbound pass into a rest frame in which the distributions are nearly isotropic requires a transformation velocity that is consistent with the local solar wind velocity if one assumes that these particles are primarily singly ionized with a mass of 18 +/- 6 atomic mass units. The existence of a frame of reference in which these water-group ions were isotropic implies that they underwent strong pitch angle scattering after their ionization. Particle energies in the rest frame extend to substantially higher values than would be expected if these ions were locally ionized and then picked up by the solar wind, implying that the ions were accelerated or heated. The derived ion density, approximately 0.1 per cubic centimeter, is consistent with a crude model for the production and transport of pickup ions.  相似文献   
42.
Global Oscillation Network Group data reveal that the internal structure of the sun can be well represented by a calibrated standard model. However, immediately beneath the convection zone and at the edge of the energy-generating core, the sound-speed variation is somewhat smoother in the sun than it is in the model. This could be a consequence of chemical inhomogeneity that is too severe in the model, perhaps owing to inaccurate modeling of gravitational settling or to neglected macroscopic motion that may be present in the sun. Accurate knowledge of the sun's structure enables inferences to be made about the physics that controls the sun; for example, through the opacity, the equation of state, or wave motion. Those inferences can then be used elsewhere in astrophysics.  相似文献   
43.
Contrary to the prediction of the Z-scheme model of photosynthesis, experiments demonstrated that mutants of Chlamydomonas containing photosystem II (PSII) but lacking photosystem I (PSI) can grow photoautotrophically with O2 evolution, using atmospheric CO2 as the sole carbon source. Autotrophic photosynthesis by PSI-deficient mutants was stable both under anaerobic conditions and in air (21 percent O2) at an actinic intensity of 200 microeinsteins per square meter per second. This PSII photosynthesis, which was sufficient to support cell development and mobility, may also occur in wild-type green algae and higher plants. The mutants can survive under 2000 microeinsteins per square meter per second with air, although they have less resistance to photoinhibition.  相似文献   
44.
An Artificial Neural Network (ANN) and an electronic nose, AromaScan, were used to predict the piggery odour concentrations emanating from an effluent pond and to develop a confident, rapid, and cost-effective technique for odour measurement. Odour samples from five different piggery effluent ponds were analysed using the AromaScan and dynamic dilution olfactometry. The resulting sensor data were used to train the artificial neural network to correlate the responses to the odour concentrations measured by olfactometry. Effectiveness was evaluated through simulation with various pre-processing techniques and network architectures. The simulation results have shown that a two-layer back-propagation neural network, which has a tan-sigmoid transfer function in the hidden layer and a linear transfer function in the output layer, could be trained to predict piggery odour concentrations with high value of the correlation coefficient R of 0·984 under the best network performance. The results from the application of scaling and principal component analysis suggest that these techniques are necessary not only to avoid the failure of the network caused by saturation but also to enhance performance. An early stopping technique was shown to provide benefits to the network performance in terms of a decrease in computation time and overfitting. It was found that the optimal number of hidden neurons for the network was 20. Odour concentration of unknown samples were able to be predicted with significant accuracy.  相似文献   
45.
Objective To evaluate the effectiveness of two insulin doses to maintain an acceptable range of blood glucose concentrations (70–200 mg dL?1) in the peri‐operative period in diabetic dogs. Animals Twenty‐four diabetic dogs with a median weight of 20.6 kg and a median age of 8 years old. Methods The dogs were randomly assigned to receive either 25 or 100% of their normal insulin dose subcutaneously on the morning of surgery. The anesthetic and feeding protocols were standardized. On the day before surgery, venous blood was collected for measurement of β‐hydroxybutyrate, cholesterol, glucose, glycosylated hemoglobin, hematocrit, total plasma protein and urea nitrogen. On the day of surgery, blood glucose concentrations were measured prior to anesthesia, prior to the start of surgery, 1 and 2 hours after beginning of surgery, 1 hour after extubation, at 16 : 00 hours and at 20 : 00 hours. β‐hydroxybutyrate concentrations were measured at 20 : 00 hours that day. At 08 : 00 hours the following day, β‐hydroxybutyrate and glucose concentrations were measured. The significance of differences between groups was tested with Wilcoxon's two‐tailed rank‐sum test, Chi‐square test and Fisher's exact test. Results There were no differences in insulin treatments, clinical signs, concurrent diseases and most clinicopathological parameters between the two groups of dogs at entry to the study. The 25% dose group had blood glucose values of 296 (102–601) mg dL?1 at 16 : 00 hours and 429 (97–595) mg dL?1 at 20 : 00 hours on the day of surgery. The 100% insulin dose group had lower corresponding values of 130 (55–375) mg dL?1 (p = 0.04) and 185 (51–440) mg dL?1 (p = 0.004). No other differences (p < 0.05) were detected between the two groups. Conclusions The administration of a full dose of insulin is only marginally advantageous for reducing glucose to normal (70–120 mg dL?1) after anesthesia but neither dose consistently induced glycemic values in an acceptable range (70–200 mg dL?1) or normoketonemia. Clinical relevance Blood glucose should be measured immediately before anesthesia and periodically throughout the peri‐operative period in all diabetic dogs because presurgical subcutaneous administration of 25 or 100% of the normal insulin dose resulted in unpredictable blood glucose concentrations.  相似文献   
46.
Our purpose was to assess the accuracy and precision of a point of care hemoglobinometer (HemoCue‐B hemoglobin photometer) for measuring hemoglobin concentration in horse blood. Samples of jugular venous blood from 12 healthy adult horses were collected in EDTA. In order to test the device over a wide range of values, each sample was divided into nine aliquots, and autologous plasma was added or removed from the aliquots to produce blood with PCV values that approximated 5, 10, 20, 30, 40, 50, 60, 70, and 80%, respectively. The aliquots were rocked to ensure mixing of plasma and cells. Then hemoglobin by HemoCue‐B (HbHQ) and hemoglobin by the cyanmethemoglobin method (HbCY) were measured on each aliquot. The PCV of each aliquot was also measured and this value was used for subsequent analyses. To test repeatability, hemoglobin was measured twice by the HemoCue‐B on approximately 40% samples. Samples with HbHQ >25.4 g dL?1 required dilution prior to analysis. HbCY ranged from 1.6 to 33.4 g dL?1. After regression, HbCY = ?0.16 + 1.04 HbHQ (n = 101; r2 = 99.6%). By inspection of a modified Bland‐Altman plot, HbHQ values <16 g dL?1 closely approximated HbCY; however, at greater values, HbHQ underestimated HbCY by as much as 3.2 g dL?1. The difference between repeated measurements with the HemoCue‐B was 0.02 ± 0.16 g dL?1 (mean ± SD; n = 10) and nonsignificant. After regression, PCV = ?0.76 + 2.78 HbHQ (n = 101; r2 = 99.4%). We conclude that HemoCue‐B can be used to measure hemoglobin concentration in horse blood, and that it is accurate when hemoglobin is <16 g dL?1. PCV can be estimated by multiplying HbHQ by 2.8 and then subtracting 0.8.  相似文献   
47.
48.
Sensitivity of boreal forest carbon balance to soil thaw   总被引:6,自引:0,他引:6  
We used eddy covariance; gas-exchange chambers; radiocarbon analysis; wood, moss, and soil inventories; and laboratory incubations to measure the carbon balance of a 120-year-old black spruce forest in Manitoba, Canada. The site lost 0.3 +/- 0.5 metric ton of carbon per hectare per year (ton C ha-1 year-1) from 1994 to 1997, with a gain of 0.6 +/- 0.2 ton C ha-1 year-1 in moss and wood offset by a loss of 0.8 +/- 0.5 ton C ha-1 year-1 from the soil. The soil remained frozen most of the year, and the decomposition of organic matter in the soil increased 10-fold upon thawing. The stability of the soil carbon pool ( approximately 150 tons C ha-1) appears sensitive to the depth and duration of thaw, and climatic changes that promote thaw are likely to cause a net efflux of carbon dioxide from the site.  相似文献   
49.
Summary The anaesthetic effects of intravenous tiletamine-zolazepam 6.6 mg/kg-ketamine 6.6 mg/kg (TK) and tiletamine-zolazepam 6.6 mg/kg-ketamine 6.6 mg/kg-xylazine 0.11 mg/kg (TKX) were evaluated in six wethers. Heart rate, respiration rate, arterial blood pressure, and the electrocardiogram were monitored during anaesthesia. Analgesia was tested by electrical stimulation in the left flank. Atropine (0.03 mg/kg) was given intramuscularly before induction, but after recording of baseline heart rate and respiratory rate. The duration of analgesia was 28.7 ± 6.9 min with TK and 82.8 ± 26.6 min with TKX. Heart rate increased significantly within 5 min after TK or TKX administration. Respiratory rate remained unchanged after TK administration, but increased significantly from 5 to 45 min after TKX administration. Arterial blood pressure decreased significantly at 15 min with TK and 30 min with TKX. Sheep remained recumbent for 201 min with TK and 166 min with TKX. All recovered uneventfully. We conclude that either TK or TKX may be used for anaesthetising sheep.  相似文献   
50.
The efficacy of oocyte selection for in vitro embryo production depends on the abundance and diameter of follicles, cumulus layers around the oocytes and subsequent fertilization. Application of `ovum pick-up' technique allows us to utilize partially matured oocytes for embryo production even from juvenile subjects. To compare their developmental competence, oocytes derived from lambs and ewes and cultured in maturation medium for up to 26 h were assessed at 2 h intervals by confocal microscopy after chromatin and microtubulin-specific fluorochrome labelling. Lamb oocytes reached second meiotic metaphase (MII) at lower numbers at 24 h (60.0%) and 26 h (28.6%) whereas 85.7% of adult-derived oocytes attained MII status by 24 h of maturation. Radiolabelling of oocyte proteins revealed higher incorporation of [35S-]-methionine and [35S]-cysteine in adult-derived oocytes compared to lamb oocytes. Although the cleavage rate of lamb oocytes was similar to that of ewe oocytes, the proportion reaching blastocyst stage was significantly lower (p < 0.05) in the lamb-derived oocytes. However, blastocysts from both types of oocytes displayed similar cell lineage allocations to inner cell mass and trophectoderm.  相似文献   
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