Vascular Endothelial (VEGF) and Epithelial Growth Factor (EGF) as Well as Platelet‐Activating Factor (PAF) and Receptors are Expressed in the Early Pregnant Canine Uterus     

S Schäfer‐Somi  S Sabitzer  D Klein  E Reinbacher  H Kanca  HB Beceriklisoy  OA Aksoy  I Kucukaslan  HC Macun  S Aslan 《Reproduction in domestic animals》2013,48(1):20-26

The aim of this study was to investigate the course of expression of platelet‐activating factor (PAF), PAF‐receptor (PAF‐R), epidermal growth factor (EGF), EGF‐R, vascular endothelial growth factor (VEGF), VEGF‐R1 and VEGF‐R2 in uterine tissue during canine pregnancy. For this purpose, 20 bitches were ovariohysterectomized at days 10–12 (n = 10), 18–25 (n = 5) and 28–45 (n = 5) days after mating, respectively. The pre‐implantation group was proven pregnant by embryo flushing of the uterus after the operation, the others by sonography. Five embryo negative, that is, non‐pregnant, bitches in diestrus (day 10–12) served as controls. Tissue samples from the uterus (placentation sites and horn width, respectively) were excised and snap‐frozen in liquid nitrogen after embedding in Tissue Tec^®. Extraction of mRNA for RT‐PCR was performed with Tri‐Reagent. In the embryos, mRNA from all factors except VEGF was detected. In the course of pregnancy, significantly higher expression of PAF and PAFR as well as VEGF and VEGFR2 during the pre‐implantation stage than in all other stages and a strong upregulation of EGF during implantation were characteristic. The course of EGF was in diametrical opposition to the course of the receptor. These results point towards an increased demand for VEGF, EGF and PAF during the earliest stages of canine pregnancy.  相似文献   

Conformity and controversies in the diagnosis,staging and follow‐up evaluation of canine nodal lymphoma: a systematic review of the last 15 years of published literature          下载免费PDF全文

L. Marconato  G. A. Polton  S. Sabattini  M. Dacasto  O. A. Garden  I. Grant  T. Hendrickx  J. Henriques  G. Lubas  E. Morello  D. Stefanello  S. Comazzi 《Veterinary and comparative oncology》2017,15(3):1029-1040

Diagnostic methods used in the initial and post‐treatment evaluation of canine lymphoma are heterogeneous and can vary within countries and institutions. Accurate reporting of clinical stage and response assessment is crucial in determining the treatment efficacy and predicting prognosis. This study comprises a systematic review of all available canine multicentric lymphoma studies published over 15 years. Data concerning diagnosis, clinical stage evaluation and response assessment procedures were extracted and compared. Sixty‐three studies met the eligibility criteria. Fifty‐five (87.3%) studies were non‐randomized prospective or retrospective studies. The survey results also expose variations in diagnostic criteria and treatment response assessment in canine multicentric lymphoma. Variations in staging procedures performed and recorded led to an unquantifiable heterogeneity among patients in and between studies, making it difficult to compare treatment efficacies. Awareness of this inconsistency of procedure and reporting may help in the design of future clinical trials.  相似文献   

梅花切花圃的建立和优质切花栽培技术初探   总被引：1，自引：1，他引：0  

何定萍  秦琴  陈柯宇  李庆生  李和平 《北京林业大学学报》2010,(Z2)

阐述了我国首个梅花切花生产基地的建立,并重点论述了梅花切花的各项栽培技术措施及注意事项,为梅花切花今后更进一步的发展提供了理论基础。  相似文献   

Comparisons between cats with normal and increased fPLI concentrations in cats diagnosed with inflammatory bowel disease     

Bailey S  Benigni L  Eastwood J  Garden OA  McMahon L  Smith K  Steiner JM  Suchodolski JS  Allenspach K 《The Journal of small animal practice》2010,51(9):484-489

Objectives : The aim of this study was to compare age, serum albumin and cobalamin concentrations, serum alanine amino transferase and alkaline phosphatase activities, feline inflammatory bowel disease clinical disease activity index, pancreatic ultrasound findings, intestinal histopathology scores, outcome, treatment and clinical response between cats diagnosed with inflammatory bowel disease with normal or increased serum feline pancreatic lipase immunoreactivity concentrations. Methods : Medical records for 23 cats diagnosed with inflammatory bowel disease and with serum feline pancreatic lipase immunoreactivity concentrations available were reviewed. Three groups were compared; cats with serum feline pancreatic lipase immunoreactivity concentrations 2·0 to 6·8 µg/l (group A), 6·9 to 11·9 µg/l (group B) and ≥12·0 µg/l (group C). Results : Sixteen of the 23 cats had increased serum feline pancreatic lipase immunoreactivity concentrations; 9 cats in group B and 7 cats in group C. The remaining seven cats were in group A. Cats with serum feline pancreatic lipase immunoreactivity concentrations ≥12·0 µg/l had significantly lower median serum albumin and cobalamin concentrations. No significant differences were identified between the three groups for age, serum alanine amino transferase and alkaline phosphatase activities, feline inflammatory bowel disease clinical disease activity index, pancreatic ultrasound findings, intestinal histopathology scores, clinical outcome, treatment or clinical response. Clinical Significance : Hypoalbuminaemia and hypocobalaminaemia were more frequently observed in cats with serum feline pancreatic lipase immunoreactivity concentrations ≥12·0 µg/l.  相似文献   

Differential growth response of Rytidosperma species (wallaby grass) to phosphorus application and its implications for grassland management          下载免费PDF全文

H. A. Waddell  R. J. Simpson  B. Henderson  M. H. Ryan  H. Lambers  D. L. Garden  A. E. Richardson 《Grass and Forage Science》2016,71(2):245-258

Rytidosperma species (formerly Austrodanthonia) are native grasses common in temperate grasslands of southern Australia. Nine Rytidosperma species, Lolium perenne and Bromus hordeaceus were grown as microswards in pots in a glasshouse, and their growth response to six levels of applied P was measured. Shoot yield differed up to twofold between the highest‐ and lowest‐yielding Rytidosperma species. Some Rytidosperma species were slow growing with minimal ability to respond to increased soil P availability. However, three species, Rytidosperma duttonianum, Rytidosperma racemosum and Rytidosperma richardsonii, had a similar shoot yield to L. perenne. Species that grew well at high P also grew well at low P, except B. hordeaceus, which was the lowest‐yielding species at low P, but had among the highest yields at high P. No species showed evidence of P toxicity. The species exhibited a range in critical external P requirement (i.e. amount of P applied for 90% maximum yield). Among the fast‐growing Rytidosperma species, R. richardsonii was notable because it had a low critical external P (16·3 mg P pot^?1) and high agronomic P‐use efficiency (94·1 g DW g^?1 P applied). In contrast, R. duttonianum had a higher critical external P requirement (22·4 mg P pot^?1) and lower agronomic P‐use efficiency (85 g DW g^?1 P applied). It was concluded that it is important to know which Rytidosperma species are present in a grassland to understand how it may respond to P fertilization. The results help to explain the diverse opinions expressed about the productivity of pastures containing Rytidosperma species.  相似文献   

ACVIM consensus statement on the diagnosis of immune‐mediated hemolytic anemia in dogs and cats     

Oliver A. Garden  Linda Kidd  Angela M. Mexas  Yu‐Mei Chang  Unity Jeffery  Shauna L. Blois  Jonathan E. Fogle  Amy L. MacNeill  George Lubas  Adam Birkenheuer  Simona Buoncompagni  Julien R. S. Dandrieux  Antonio Di Loria  Claire L. Fellman  Barbara Glanemann  Robert Goggs  Jennifer L. Granick  Dana N. LeVine  Claire R. Sharp  Saralyn Smith‐Carr  James W. Swann  Balazs Szladovits 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):313-334

Immune‐mediated hemolytic anemia (IMHA) is an important cause of morbidity and mortality in dogs. IMHA also occurs in cats, although less commonly. IMHA is considered secondary when it can be attributed to an underlying disease, and as primary (idiopathic) if no cause is found. Eliminating diseases that cause IMHA may attenuate or stop immune‐mediated erythrocyte destruction, and adverse consequences of long‐term immunosuppressive treatment can be avoided. Infections, cancer, drugs, vaccines, and inflammatory processes may be underlying causes of IMHA. Evidence for these comorbidities has not been systematically evaluated, rendering evidence‐based decisions difficult. We identified and extracted data from studies published in the veterinary literature and developed a novel tool for evaluation of evidence quality, using it to assess study design, diagnostic criteria for IMHA, comorbidities, and causality. Succinct evidence summary statements were written, along with screening recommendations. Statements were refined by conducting 3 iterations of Delphi review with panel and task force members. Commentary was solicited from several professional bodies to maximize clinical applicability before the recommendations were submitted. The resulting document is intended to provide clinical guidelines for diagnosis of, and underlying disease screening for, IMHA in dogs and cats. These should be implemented with consideration of animal, owner, and geographical factors.  相似文献   

In vitro differentiation of bovine bone marrow‐derived mesenchymal stem cells into male germ cells by exposure to exogenous bioactive factors          下载免费PDF全文

J Cortez  J Bahamonde  M De los Reyes  J Palomino  CG Torres  OA Peralta 《Reproduction in domestic animals》2018,53(3):700-709

Mesenchymal stem cells (MSC) are multipotent progenitor cells defined by their ability to self‐renew and give rise to differentiated progeny. Previous studies have reported that MSC may be induced in vitro to develop into different types of specialized cells including male gametes. In vitro gamete derivation technology has potential applications as an alternative method for dissemination of elite animal genetics, production of transgenic animals and conservation of endangered species. This study aimed at investigating the in vitro effect of BMP4, TGFβ1 and RA on the potential for germ cell (GC) differentiation of bovine foetal MSC (bfMSC) derived from bone marrow (BM). The effect of BMP4, TGFβ1 and RA was analysed on the expression of pluripotent, GC and male GC markers on bfMSC during a 21‐day culture period. bfMSC cultured under in vitro conditions expressed OCT4, NANOG and DAZL, but lacked expression of mRNA of VASA, STELLA, FRAGILIS, STRA8 and PIWIL2. Treatment with exogenous BMP4 and TGFβ1 induced a transient increase (p < .05) in DAZL and NANOG mRNA levels, respectively. However, exposure to RA was more effective in increasing (p < .05) expression of DAZL and regulating expression of OCT4 and mRNA levels of NANOG. These data suggest that bfMSC may possess potential for early GC differentiation, where OCT4, NANOG and specially DAZL may play significant roles in controlling progression along the GC lineage.  相似文献   

南亚热带丘陵3种人工林群落的生物量及净初级生产力   总被引：4，自引：0，他引：4  

曾小平  蔡锡安  赵平  饶兴权  邹碧  周丽霞  林永标  傅声雷 《北京林业大学学报》2008,30(6):148-152

该文采用收获法和标准木法研究了南亚热带3种人工林群落:马占相思林、针叶林(马尾松+杉木)、木荷林(木荷+红荷)的生物量及净初级生产力,为揭示鹤山丘陵人工林的结构功能规律及全球变化生态学的研究提供基础资料。结果表明,林分现存生物量由大到小依次为:马占相思林(176.03 t/hm2)、木荷林(122.91 t/hm2)、针叶林(104.93 t/hm2)。在林分各层次的分配比例中,乔木层所占比例最大,为95.1%～98.7%;灌木层和草本层所占比例较小,尤其是草本层,灌木层为0.9%～4.5%,草本层为0.4%。净初级生产力（NPP）由大到小依次为:马占相思林(25.32 t/(hm2·a))、木荷林(17.93 t/(hm2·a))、针叶林(15.8 t/(hm2·a)),与林分生物量大小呈同样的规律。木荷林乔木层和灌木层的生物量增量所占比例高于另外两种林分,群落结构较复杂,生物量积累呈明显的增长趋势。   相似文献   

构筑无锡城市生态走廊网络——无锡市绿地系统规划研究   总被引：6，自引：3，他引：3  

刘颂  刘滨谊  邬秉左 《中国城市林业》2004,2(5):9-12

本文以生态优先为原则结合无锡市的自然人文特点、城市建设及其发展趋势,构建了无锡市城市生态走廊框架。    相似文献   

热胁迫下羽叶薰衣草植物腺毛发育的超微结构     

黄珊珊  廖景平 《广西农业生物科学》2010,(1):97-102

本研究利用透射电子显微镜观察热胁迫下羽叶薰衣草腺毛超微结构变化,结果表明：40℃热胁48h后,腺毛细胞中参与合成与分泌的主要细胞器受到严重破坏。质体变形,缺少基质和嗜锇物质;线粒体缺少嵴,液泡化严重;小泡不正常地融合在一起,并靠细胞壁分布;内质网呈链条状,片层似球形,核糖体显著地附在内质网片层上;细胞核出现大量的纤维状颗粒物质;分泌时期的质膜和细胞器膜等扭曲变形。说明热胁迫影响腺毛的发育与精油的分泌,从而进一步影响精油的产量和质量。  相似文献