The Kendall Oration--Veterinarians in society--some future directions     

Murray G 《Australian veterinary journal》2004,82(7):394-397

Every four years a distinguished veterinarian is invited to provide the profession with a contemporary perspective on our craft. This year Australian Chief Veterinary officer Gardner Murray delivered a truly international viewpoint through the Kendall Oration at AVA's National Conference in Canberra.  相似文献   

Equine adenovirus 1 infection of hospitalised and healthy foals and horses     

Bell SA  Leclere M  Gardner IA  Maclachlan NJ 《Equine veterinary journal》2006,38(4):379-381

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Fell Pony syndrome in a pony in North America     

Gardner RB  Hart KA  Stokol T  Divers TJ  Flaminio MJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(1):198-203

A 5-week-old Fell Pony colt was examined for fever, lethargy, and anemia. The colt had been lethargic for 1 week before examination, had continued to nurse, had a temperature of 104°F (40°C), and was treated with ceftiofur (5 mg/kg IM q12h). Approximately 36 hours before examination, the colt developed watery diarrhea. Blood work performed by the referring veterinarian on the day of admission revealed a PCV of 10%.  相似文献   

Utility of Urinary Aldosterone Measurement in Quantitating RAAS Activation     

CE Atkins  AC Lantis  MK Ames  SY Gardner 《Journal of veterinary pharmacology and therapeutics》2012,35(5):512-515

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Validation of real-time polymerase chain reaction tests for diagnosing feline immunodeficiency virus infection in domestic cats using Bayesian latent class models     

Morton JM  McCoy RJ  Kann RK  Gardner IA  Meers J 《Preventive veterinary medicine》2012,104(1-2):136-148

The objectives of the current study were to estimate the sensitivity and specificity of three real-time polymerase chain reaction (PCR) tests for diagnosis of feline immunodeficiency virus (FIV) infection in domestic cats, both individually and when interpreted in series with one of two serological tests, separately in populations of cats at low and high risk of being infected with FIV. One PCR test targeted the pol gene and two targeted the gag gene of FIV. For comparison, sensitivities and specificities of the individual serological tests (IDEXX SNAP(?) test and AGEN Simplify(?) test) were also estimated. The study populations consisted of domestic cats thought to be not vaccinated against FIV. Low-risk (males aged 4 years or less and females; n=128) and high-risk (males over 4 years; n=128) cats were selected from those where blood samples were submitted to a commercial clinical pathology service. Bayesian latent class models were used to obtain posterior probability distributions for sensitivity and specificity for each test, based on prior distributions obtained from three experts. Medians of the posterior sensitivity distributions for the PCR tests based on the pol gene and two regions of the gag gene tests ranged from 0.85 to 0.89, compared to 0.89-0.97 for the two serological tests. The medians of posterior specificity distributions for these PCR tests were 0.94-0.96, and 0.95-0.97 for the serological tests. In contrast, the PCR based on one region of the gag gene had lower median sensitivity. Sensitivities of combinations of these serological and PCR tests interpreted in series were low; medians of posterior sensitivity distributions ranged from 0.75 to 0.83. Relative to the low-risk population, median sensitivities in the high-risk population were lower for all tests other than the AGEN Simplify(?) test; specificities were similar in both populations. We conclude that the sensitivities of the two PCR tests based on the pol gene and two regions of the gag gene, respectively, in non-vaccinated cats are probably lower than the sensitivities of the two serological tests we assessed. We do not recommend screening cats whose FIV vaccination status is uncertain with one of these serological tests and then testing positives with one of these PCR tests because in non-vaccinates, the sensitivities of combinations of these serological and PCR tests interpreted in series are low. Assessment of the validity of these PCR assays in FIV-vaccinated cats is required.  相似文献   

Protocol: High-throughput and quantitative assays of auxin and auxin precursors from minute tissue samples     

X Liu  AD Hegeman  G Gardner  JD Cohen 《Plant methods》2012,8(1):31-17

ABSTRACT: BACKGROUND: The plant hormone auxin, indole-3-acetic acid (IAA), plays important roles in plant growth and development. The signaling response to IAA is largely dependent on the local concentration of IAA, and this concentration is regulated by multiple mechanisms in plants. Therefore, the precise quantification of local IAA concentration provides insights into the regulation of IAA and its biological roles. Meanwhile, pathways and genes involved in IAA biosynthesis are not fully understood, so it is necessary to analyze the production of IAA at the metabolite level for unbiased studies of IAA biosynthesis. RESULTS: We have developed high-throughput methods to quantify plant endogenous IAA and its biosynthetic precursors including indole, tryptophan, indole-3-pyruvic acid (IPyA), and indole-3-butyric acid (IBA). The protocol starts with homogenizing plant tissues with stable-labeled internal standards added, followed by analyte purification using solid phase extraction (SPE) tips and analyte derivatization. The derivatized analytes are finally analyzed by selected reaction monitoring on a gas chromatograph-mass spectrometer (GC-MS/MS) to determine the precise abundance of analytes. The amount of plant tissue required for the assay is small (typically 2--10 mg fresh weight), and the use of SPE tips is simple and convenient, which allows preparation of large sets of samples within reasonable time periods. CONCLUSIONS: The SPE tips and GC-MS/MS based method enables high-throughput and accurate quantification of IAA and its biosynthetic precursors from minute plant tissue samples. The protocol can be used for measurement of these endogenous compounds using isotope dilution, and it can also be applied to analyze IAA biosynthesis and biosynthetic pathways using stable isotope labeling. The method will potentially advance knowledge of the role and regulation of IAA.  相似文献   

Passive transfer and rate of decay of maternal antibody against African horse sickness virus in South African Thoroughbred foals     

J. E. Crafford  C. W. Lourens  I. A. Gardner  N. J. Maclachlan  A. J. Guthrie 《Equine veterinary journal》2013,45(5):604-607

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Haematological and biochemical findings in bovine post-parturient haemoglobinuria and the accompanying Heinz-body anaemia     

D.E. Gardner Ph.D. B.V.Sc. Dip.Microbiol. M.A.C.V.SC.  D. Martinovich B.V.Sc. M.S. M.R.C.V.S. M.A.C.-V.Sc. Dip.Agric.  D.A. Woodhouse A.I.M.L.T. 《New Zealand veterinary journal》2013,61(6):117-122

Sarcocysts morphologically similar to Sarcocystis muris were observed in the skeletal muscles of mice dosed with free isosporan sporocysts recovered from the faeces of naturally infected cats. Kittens fed these mice shed similar sporocysts measuring 8.7 ±0.1 x 10.8 ±0.1 μm in their faeces five to six days later. Feeding these sporocysts to mice and rats resulted in the formation of identical sarcocysts in mice, but not rats. Attempts to induce similar infections in mice using naturally acquired isolates of I. felis were unsuccessful.  相似文献   

Soil and Atmospheric Inputs to PAH Concentrations in Salt Marsh Plants     

Alison W. Watts  Thomas P. Ballestero  Kevin H. Gardner 《Water, air, and soil pollution》2008,189(1-4):253-263

Polycyclic aromatic hydrocarbons (PAHs) were measured in two wetland plant species grown outdoors in pots of sediment contaminated with up to 730,000 μg/kg total PAHs. After approximately 3 months, the plants were harvested, cleaned, and analyzed for an expanded suite of PAHs which included both the 16 priority PAHs and 22 alkyl PAH homologs. Sediment and air samples were also collected and analyzed. PAH compounds were detected in all of the samples, with the highest concentrations in the contaminated sediment. The root sample concentrations were generally about one order of magnitude lower than that of the sediment, and were strongly correlated with the concentration in the sediment in which they were grown. The concentrations in foliage were much lower and did not correlate with sediment concentration. Concentrations of low molecular weight PAH compounds detected in the foliage were not significantly lower in plants grown in control sediments, suggesting that the sediment is not the primary source of these PAHs. Several high molecular weight PAHs were detected only in plants grown in contaminated sediment. Plants of both species grown in control sediment were larger than plants grown in contaminated sediment.  相似文献   

Effect of body condition on consumption of pine needles (Pinus ponderosa) by beef cows     

Pfister JA  Panter KE  Gardner DR  Cook D  Welch KD 《Journal of animal science》2008,86(12):3608-3616

We determined whether cows in low (LBC) or high body condition (HBC) would consume different amounts of green pine needles (Pinus ponderosa). Cows (mature; open Hereford and Hereford x Angus) were fed a maintenance basal diet (alfalfa pellets) for Exp. 1 and 2; during Exp. 3 and 4, cows were fed high-protein and high-energy diets, respectively. Experiment 5 was a grazing study on rangeland during winter in South Dakota; diets were determined by using bite counts. Mean BCS (1 = emaciated, 9 = obese) was 7.5 for HBC cows and <4.0 for LBC cows during the experiments. During Exp. 1, LBC cows consumed more (P = 0.001) pine needles than did HBC cows (5.5 +/- 0.25 vs. 1.0 +/- 0.14 g/kg of BW daily, respectively). During Exp. 2, there was a day x treatment interaction (P = 0.001) as LBC cows consumed variable, but greater, amounts of pine needles than did HBC cows (3.7 +/- 0.19 vs. 1.3 +/- 0.12 g/kg of BW daily, respectively). When fed a high-protein/low-energy diet, LBC cows ate more (P = 0.04) pine needles than did HBC cows. When fed a low-protein/high-energy diet, there was a day x treatment interaction (P = 0.001) because LBC cows consumed more pine needles than did HBC cows for the first 3 d of the study, and then consumption by LBC animals decreased during the last 4 d. These experiments suggest that the protein:energy ratio may be an important factor in the ability of cows to tolerate terpenes, and that cows were not able to sustain an increased quantity of needle consumption on a low-protein diet. During the 25-d grazing study, there was a day x treatment interaction (P = 0.001) as LBC animals selected more pine needles (up to 25% of daily bites) on some days compared with HBC cows. Weather influenced pine needle consumption because pine needle bites by LBC cows were related (r(2) = 0.60; P = 0.001) to days of greater snow depth and lower minimum daily temperatures. Both LBC and HBC cows increased selection of pine needles from trees during cold, snowy weather, but the magnitude of the increase was greater for LBC cows. The LBC cows consumed more pine needles than did HBC cows in all experiments, except when cows were fed a low-protein diet. This study indicates that both body condition and protein intake are important factors in pine needle consumption.  相似文献