Distribution and temporal prevalence of Theileria orientalis major piroplasm surface protein types in eastern Australian cattle herds     

GJ Eamens  G Bailey  JR Gonsalves  C Jenkins 《Australian veterinary journal》2013,91(8):332-340

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Comparison of two selective media for the recovery, isolation, enumeration and differentiation of Rhodococcus equi     

Muscatello G  Gilkerson JR  Browning GF 《Veterinary microbiology》2007,119(2-4):324-329

The use of selective media to facilitate the isolation of Rhodococcus equi from environmental and clinical samples has aided studies of the ecology of R. equi and the epidemiology of disease caused by R. equi. Here, we compared the efficacy of two selective media (NANAT and modified CAZ-NB) for the recovery of six defined strains of R. equi and for the isolation and enumeration of both avirulent and virulent R. equi from 60 paired soil samples from horse farms using colony blotting and DNA hybridisation. No difference was found between the two media in the recoverability of defined strains of R. equi or the proportion of soil cultures positive for R. equi or virulent R. equi. NANAT medium was significantly less inhibitory of bacterial growth from soil culture compared to mCAZ-NB (P = 0.001), but there was no difference between the media in the number of R. equi colonies recovered. Soil cultured on mCAZ-NB medium yielded a significantly greater number of virulent R. equi colonies than NANAT (P = 0.03). The proportion of R. equi that were virulent in soil cultures on mCAZ-NB (32%) was more than three times that seen in cultures on NANAT (9%). Thus modified CAZ-NB appeared to be a better selective media for studies where the optimal recovery of virulent R. equi is required, such as in studies of the gastrointestinal carriage of virulent R. equi and of subclinically infected foals.  相似文献   

The Use of Colonic Irrigation to Control Fecal Incontinence in Dogs With Colostomies     

Fred A. Williams  JR  DVM  Ronald M. Bright  DVM  MS  Diplomate ACVS    Gregory B. Daniel  DVM  MS  Diplomate ACVR    Kevin A. Hahn  DVM  PhD    Steve A. Patton  DVM  MS  Diplomate ACVP 《Veterinary surgery : VS》1999,28(5):348-354

OBJECTIVE: To determine if once-daily colonic irrigation results in fecal continence for a 24-hour period in dogs with colostomies and if colonic volume increased in response to the irrigation. STUDY DESIGN: A prospective controlled experimental study. ANIMALS: Four intact male and one intact female mixed breed dogs. MATERIALS: All dogs received left end-on paralumbar colostomies. Four dogs received once-daily colonic irrigation for 8 weeks, whereas the control dog did not. Daily fecal weights were recorded for the length of the study in all dogs. Barium enema studies and volumetric studies were used to determine colonic volumes. RESULTS: Daily fecal weights were significantly decreased in treatment dogs compared with the control dog. Colonic volume increased in irrigated dogs in response to daily irrigation over the 8 week period of the study. CONCLUSIONS: Colonic irrigation resulted in significantly decreased fecal production over a 24-hour period. Therefore management of dogs with colostomies would be more practical and cost effective. It did not result in complete fecal continence in this study. Further clinical studies are indicated to determine if longer periods of irrigation would result in complete continence.  相似文献   

Epidemiological studies of equine herpesvirus 1 (EHV-1) in Thoroughbred foals: a review of studies conducted in the Hunter Valley of New South Wales between 1995 and 1997.     

J R Gilkerson  J M Whalley  H E Drummer  M J Studdert  D N Love 《Veterinary microbiology》1999,68(1-2):15-25

Sero-epidemiological studies conducted between 1995 and 1997 on two large Thoroughbred stud farms in the Hunter Valley of NSW showed clear evidence of EHV-1 infection in foals as young as 30 days of age. Similarly, serological evidence suggested that these foals were infected with EHV-1 from their dams or from other lactating mares in the group, with subsequent foal to foal spread of infection prior to weaning. These studies also provided evidence of EHV-1 infection of foals at and subsequent to weaning, with foal to foal spread of EHV-1 amongst the weanlings. These data indicated that the mare and foal population was a reservoir of EHV-1, from which new cases of infection propagated through the foal population both before and after weaning. The results of these studies support the long standing management practices of separating pregnant mares from other groups of horses to reduce the incidence of EHV-1 abortion. Also, these results have important implications for currently recommended vaccination regimens, as the efficacy of vaccination in already latently infected horses is unknown.  相似文献   

The dietary regime of detritivorous fish from the River Jacaré Pepira, Brazil     

M. M. Vaz  M. Petrere JR  L. A. Martinelli  A. A. Mozeto 《Fisheries Management and Ecology》1999,6(2):121-132

The diet of detritivorous fish was analysed at four distinct habitats in a tropical river during the wet and dry seasons. Two methodologies were used to determine the diet of the fish: (1) the direct analysis of the stomach contents; and (2) the use of stable carbon isotopes. Both methods indicated food partitioning among the fish. The stomach contents showed what the fish had ingested when captured and the ¹³C isotope indicated what it had assimilated over a long period of time. The ¹³C isotope suggested the existence of sectional differences in the feeding of the detritivorous fish along the river. Plants employing the C₄ photosynthesis pathways seem to have little influence on the diet of these fish. This method proved to be more appropriate for the detection of the main food sources of the captured fish.  相似文献   

Artificial reef placement: a red snapper,Lutjanus campechanus,ecosystem and fuzzy rule‐based model     

J. B. SHIPLEY  J. H. COWAN JR 《Fisheries Management and Ecology》2011,18(2):154-167

Abstract This study on reef placement involves two aspects: (1) the development of a bioenergetics model for red snapper, Lutjanus campechanus (Poey), in Gulf of Mexico waters off the coast of Alabama, where significant numbers of artificial reefs are thought to exist, and (2) a fuzzy rough set model by which parameters determined from the bioenergetics model can provide a decision tool for optimally spacing artificial reefs during deployment. The bioenergetics and consumption rates of L. campechanus by age class foraging on artificial reefs in the Gulf of Mexico off the coast of Alabama provided input into the fuzzy rule‐based model. After conducting multiple simulations, highest certainty in optimal reef spacing was achieved for reef distances between 0.50 and 0.95 km such that no more than two fit within a 1‐km² area. Results can inform fisheries managers about placement of artificial reefs to affect the health and survival of reef‐associated species.  相似文献   

Effect of practical diets with different protein levels on the performance of Farfantepenaeus paulensis juveniles nursed in a zero exchange suspended microbial flocs intensive system     

E.L.C. BALLESTER  P.C. ABREU  R.O. CAVALLI  M. EMERENCIANO  L. De ABREU  W. WASIELESKY JR 《Aquaculture Nutrition》2010,16(2):163-172

Farfantepenaeus paulensis juveniles (72 ± 24 mg), were reared in a suspended microbial flocs system and fed practical diets containing increasing amounts of crude protein (250, 300, 350, 400 and 450 g kg^?1 CP). Development of microbial flocs was promoted by high aeration rates and fertilization with wheat bran and molasses. Flocs were composed of detritus in the form of flocculated matter colonized by heterotrophic bacteria, cocoid and filamentous cyanobacteria, flagellate and ciliate protozoa and rotifers. Proximate composition analysis of the suspended microbial floc showed CP levels of 304 g kg^?1. After 45 days, mean shrimp survival were above 89%, with no significant differences between treatments. Shrimp fed diets with 350 g kg^?1 or higher CP content achieved significant higher (P < 0.05) final weight (0.66–0.68 g), weight gain (0.58–0.61 g) and instantaneous growth rate (0.049–0.050), with feed conversion rates (2.17–2.30) significantly lower (P < 0.05). Results show that, when rearing is carried out in a suspended microbial flocs system, dietary CP levels can be kept at 350 g kg^?1. Furthermore, results confirm that microbial‐based systems allow shrimp culture without compromising the surrounding environment and shows the possible reduction of production costs and fish meal dependence.  相似文献   

Transduction and Transplantation of Spermatogonia into the Testis of Ram Lambs through the Extra-testicular Rete     

JR Rodriguez-Sosa  JD Silvertown  RA Foster  JA Medin  A Hahnel 《Reproduction in domestic animals》2009,44(4):612-620

Spermatogonial transplantation will provide a new way to study spermatogenesis in domestic animals, disseminate male genetics and produce transgenic animals, if efficiency can be improved. We evaluated a 'surgical' method for transplanting donor cells into testes of ram lambs, where the head of the epididymis is reflected, and a catheter introduced into the extra-testicular rete testis. We also tested transduction of ram spermatogonia with a lentiviral (LV) vector as a means to identify permanent colonization, and introduce genes into donor cells. Eight ram lambs, 11- to 13-week olds, were the recipients: in five, spermatogonia were injected into one testis, and the contralateral testis was an un-manipulated control: in two, spermatogonia were injected into one testis and the contralateral was sham-injected: in one, both testes were injected. Six lambs received spermatogonia labelled with a cell-tracking dye and these were collected 1 or 2 weeks after transplantation; three lambs received spermatogonia transduced with a LV vector driving the expression of enhanced Green Fluorescence Protein and these were collected after 2 months. Donor cells were detected by immunohistochemistry in tubules of seven of nine recipient testes. Approximately 22% of tubule cross-sections contained donor cells immediately after transplantation, and 0.2% contained virally transduced cells 2 months after transplantation. The onset of spermatogenesis was delayed, and there were lesions in both injected and sham-injected testes. Despite the effects of the surgery, elongated spermatids were present in one recipient testis 2 months after surgery. The results suggest that, after modifying the surgical and transduction techniques, this approach will be a means to produce good colonization by donor spermatogonia in sheep testes.  相似文献   

Equine amnionitis and fetal loss: The case definition for an unrecognised cause of abortion in mares     

KH Todhunter  NR Perkins  RM Wylie  C Chicken  AJ Blishen  DJ Racklyeft  G Muscatello  MC Wilson  PL Adams  JR Gilkerson  WL Bryden  AP Begg 《Australian veterinary journal》2009,87(1-2):35-38

A series of abortions occurred in mares in New South Wales during 2004 that involved similar and unusual findings on post mortem examination of aborted fetuses and fetal membranes. The term Equine Amnionitis and Fetal Loss (EAFL) was developed to describe the condition. This form of abortion had not been previously recognised in Australia. The pathology alone is not specific for EAFL and diagnosis requires demonstration of a combination of certain pathological and bacteriological features. The purpose of this paper is to describe patterns considered consistent with EAFL cases as a working case definition for use by veterinarians and veterinary pathologists in identifying future cases of EAFL. More detailed papers are in preparation to fully describe the epidemiological, histopathological, and microbiological aspects of EAFL.  相似文献   

Depredation by harbor seal and spiny dogfish in a Georges Bank gillnet fishery     

A. R. RAFFERTY  E. O. BRAZER JR  R. D. REINA 《Fisheries Management and Ecology》2012,19(3):264-272

Abstract Depredation can have economic, social and ecological impacts by decreasing fishery landings and threatening marine predator populations through increased risk of entanglement. This investigation quantified the loss of landings attributed to harbour seal, Phoca vitulina L., and spiny dogfish, Squalus acanthias L., depredation in the Georges Bank, Massachusetts, USA gillnet fishery. Approximately 1361 kg (2.38%) of fish was discarded from a total possible catch weight of nearly 57 300 kg because it was unsalable following depredation. Spiny dogfish accounted for 1134 kg (1.98%), and harbour seal accounted for 227 kg (0.40%) of discards. This represented an estimated US$2250 loss in profits from a total possible catch value of almost US$61 800, signifying a 3.64% market‐value loss during the assessment period. These results suggest that discarding due to harbour seal and spiny dogfish depredation results in a relatively small market loss to fishers, although additional information and research is needed before this can be confirmed. This study is the first on this subject in the fishery and provides baseline information on depredation rates that could be useful if harbour seal and spiny dogfish populations continue to increase.  相似文献