Mutational analysis of the tyrosine kinome in colorectal cancers     

Bardelli A  Parsons DW  Silliman N  Ptak J  Szabo S  Saha S  Markowitz S  Willson JK  Parmigiani G  Kinzler KW  Vogelstein B  Velculescu VE 《Science (New York, N.Y.)》2003,300(5621):949

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Identification and characterization of genes differentially displayed in Rosa hybrida petals during flower senescence     

Hanife Hajizadeh  Khadijeh Razavi  Younes Mostofi  Amir Mousavi  Giovanni Cacco  Zabihollah Zamani  Piergiorgio Stevanato 《Scientia Horticulturae》2011

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Multielement fingerprinting as a tool in origin authentication of PGI food products: Tropea red onion     

Furia E  Naccarato A  Sindona G  Stabile G  Tagarelli A 《Journal of agricultural and food chemistry》2011,59(15):8450-8457

Tropea red onion ( Allium cepa L. var. Tropea) is among the most highly appreciated Italian products. It is cultivated in specific areas of Calabria and, due to its characteristics, was recently awarded with the protected geographical indications (PGI) certification from the European Union. A reliable classification of onion samples in groups corresponding to "Tropea" and "non-Tropea" categories is now available to the producers. This important goal has been achieved through the evaluation of three supervised chemometric approaches. Onion samples with PGI brand (120) and onion samples not cultivated following the production regulations (80) were digested by a closed-vessel microwave oven system. ICP-MS equipped with a dynamic reaction cell was used to determine the concentrations of 25 elements (Al, Ba, Ca, Cd, Ce, Cr, Dy, Eu, Fe, Ga, Gd, Ho, La, Mg, Mn, Na, Nd, Ni, Pr, Rb, Sm, Sr, Tl, Y, and Zn). The multielement fingerprint was processed using linear discriminant analysis (LDA) (standard and stepwise), soft independent modeling of class analogy (SIMCA), and back-propagation artificial neural network (BP-ANN). The cross-validation procedure has shown good results in terms of the prediction ability for all of the chemometric models: standard LDA, 94.0%; stepwise LDA, 94.5%; SIMCA, 95.5%; and BP-ANN, 91.5%.  相似文献   

Oral administration of tepoxalin in the horse: a PK/PD study     

Giorgi M  Cuniberti B  Ye G  Barbero R  Sgorbini M  Vercelli C  Corazza M  Re G 《Veterinary journal (London, England : 1997)》2011,190(1):143-149

Tepoxalin is a non-steroidal anti-inflammatory drug with analgesic, anti-inflammatory, and antipyretic properties and has been recently introduced into veterinary medicine. The aim of this study was to evaluate the pharmacokinetic/pharmacodynamic (PK/PD) profile of tepoxalin to assess whether it would be suitable for clinical use in horses. Six female fasting/fed horses were given 10mg/kg tepoxalin orally in a cross-over study. After administration, tepoxalin underwent rapid and extensive hydrolytic conversion to its carboxylic acid metabolite RWJ-20142. In animals that had been fed, the plasma concentrations of tepoxalin were undetectable, whereas in fasting animals they were close to the limit of quantification of the method. No differences between the fasting/fed groups in RWJ-20142 plasma concentrations were shown. Tepoxalin showed a strong and long-lasting ex vivo inhibitory activity against cyclooxygenase (COX)-1, mainly due to its main metabolite RWJ-20142. Tepoxalin and RWJ-20142 do not seem to possess either COX-2 or 5-lipoxygenase inhibitory activity in the horse. These features suggest that the drug is a selective COX-1 inhibitor in horses, with no significant anti-inflammatory activity. Thus, its long term use in equine practice could be of concern.  相似文献   

Influence of shearing on oxidative stress and some physiological parameters in ewes     

Giuseppe PICCIONE  Francesco FAZIO  Stefania CASELLA  Pietro PENNISI  Giovanni CAOLA 《Animal Science Journal》2011,82(3):481-485

The aim of the present study was to evaluate the effect of shearing on physiological and oxidative stress parameters in ewes. Twenty Comisana ewes were used and divided into two groups. Ten ewes were left unshorn as a control group (Group A) and 10 ewes were shorn (Group B). All measurements were taken before and after shearing, and repeated 8 h after shearing and 1, 2, 3, 4, 5, 10 and 15 days after shearing. Reactive oxygen species (dROMs), antioxidant barrier (oxy‐adsorbent), thiol antioxidant barrier (SHp) and packed cell volume (PCV) were assessed in blood samples collected by means of jugular venipuncture. Rectal temperature (RT), respiratory rate (RR) and heart rate (HR) were also measured. Two‐way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, was used for the assessment of significant effects due to shearing and time. The statistical analysis showed significant increases (P < 0.01) of dROMs, oxy‐adsorbent, SHp, and a significant decrease (P < 0.01) of RT and RR associated with time and shearing. Our results indicate that shearing causes a change in the ewe's homeostatic balance that leads to oxidative stress.  相似文献   

Penicilliosis in an African grey parrot (Psittacus erithacus)     

Lanteri G  Sfacteria A  Macri D  Reale S  Marino F 《Journal of zoo and wildlife medicine》2011,42(2):309-312

Penicillium mycosis, or penicilliosis, is rare in animals. This report describes the clinical signs and pathological, immunohistochemical, and biomolecular findings in a case of avian penicilliosis in a Congo African grey parrot. On gross necropsy, pyogranulomatous changes were identified in the lung, liver, and kidney. Histopathology demonstrated periodic acid Schiff-(PAS) and Grocott-positive hyphae in these tissues. Immunohistochemistry confirmed the genus of the fungus and polymerase chain reaction (PCR) confirmed a high homology with Penicillium chrysogenum.  相似文献   

Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells     

Giuliano Pisoni  Patrizia D’Amelio  Francesca Sassi  Giovanni Manarolla  Licia Scaccabarozzi  Clara Locatelli  Nausicaa Mazzocchi  Mirko Baruscotti  Dario DiFrancesco  Paolo Moroni 《Veterinary journal (London, England : 1997)》2011,189(3):361-363

Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7–14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.  相似文献   

Assay of aroma active components of virgin olive oils from southern Italian regions by SPME-GC/ion trap mass spectrometry     

Benincasa C  De Nino A  Lombardo N  Perri E  Sindona G  Tagarelli A 《Journal of agricultural and food chemistry》2003,51(3):733-741

An SPME-GC/ion trap method was exploited to determine the chromatogram of volatile compounds of organic olive oils of southern Italian regions. The method is based on the assay of the terminal species of the "lipoxygenase pathway", which are present in the volatile fraction of the sampled compounds. Ethyl isobutanoate was used as internal standard in either the EI or CI ionization mode. The absolute concentration values of each analyte were evaluated through good-to-excellent calibration curves. Case studies on oils obtained from different cultivars or harvesting times are presented. The quantitative data for each compound were subjected to principal component analysis to characterize the different cultivars of this work.  相似文献   

Behavior of a fenhexamid photoproduct during the alcoholic fermentation of Saccharomyces cerevisiae     

Cabras P  Farris GA  Pinna MV  Pusino A 《Journal of agricultural and food chemistry》2004,52(26):8053-8056

The fungicide fenhexamid [N-(2,3-dichloro-4-hydroxyphenyl)-1-methylcyclohexanecarboxamide] degraded rapidly by UV or sunlight irradiation, yielding 7-chloro-6-hydroxy-2-(1-methylcyclohexyl)-1,3-benzoxazole (CHB) as a main photoproduct. CHB was isolated, and its effect on alcoholic fermentation of Saccharomyces cerevisiae was studied. The results indicate that the presence of CHB does not affect the extent of alcohol production. After 12 days, the amount of CHB in the fermentation medium decreased by ca. 65%. Only 25% of the missing CHB was recovered unchanged from yeasts, most likely because it was adsorbed on the yeast wall cell. The remaining part degraded during the fermentation process. Glucan and chitin, two potential adsorbents, which constitute yeast cell walls, exhibited affinity for CHB.  相似文献   

Effect of olive ripening degree on the oxidative stability and organoleptic properties of cv. Nostrana di Brisighella extra virgin olive oil     

Rotondi A  Bendini A  Cerretani L  Mari M  Lercker G  Toschi TG 《Journal of agricultural and food chemistry》2004,52(11):3649-3654

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