Isolation and characterization of a virus associated with arthritis of chickens   总被引：6，自引：0，他引：6  

S E Glass  S A Naqi  C F Hall  K M Kerr 《Avian diseases》1973,17(2):415-424

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Asbestiform amphibole minerals: detection and measurement of high concentrations in municipal water supplies   总被引：2，自引：0，他引：2  

P M Cook  G E Glass  J H Tucker 《Science (New York, N.Y.)》1974,185(4154):853-855

Ashestiform amphibole minerals, which have been demonstrated to be associated with human health problems, have been detected in substantial quantities in municipal water supplies taken from western Lake Superior Water. The total concentrationl of amphibole minerals in the Duluth, Minnesota, water supply, as measured by x-ray diffraction for daily samples of suspended solids averages 0.19 milligram per liter with large fluctuations due to seasonal and climatological effects on lake circulation. Electron microscopic examination of these water samples confirms the presence of asbestiform amphibole fibers. A conservatiue estimate of the fiber count for 1973 Duluth water supply samples is (1 to 30) x 10(6) amphibole fibers identifiable by electron diffraction per liter of water with a mass concentration of 1 to 30 micrograms per liter.  相似文献   

Hydrocephalus secondary to obstruction of the lateral apertures in two dogs          下载免费PDF全文

M Kent  EN Glass  AC Haley  LS Shaikh  M Sequel  U Blas‐Machado  TM Bishop  SP Holmes  SR Platt 《Australian veterinary journal》2016,94(11):415-422

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家蚕对8种除草剂农药的毒性反应   总被引：5，自引：0，他引：5  

陈小平  严欲民  黄世群  贾利琼  唐聘芳  毛业炀  唐华伦  何炳林  陈健 《西南农业学报》2000,13(3):117-120

近年来农资部门和植保部门出售的除草剂品种繁多,新药不断出现,有许多除草剂在大田作物和桑园中使用,这些除草剂农药对家蚕的毒性如何?笔者等近年来做了大田常用除草剂农药对家蚕的毒性试验,本文报道8种除草剂对家蚕的触杀毒力、安全间隔期及中毒症状,以便为蚕农科学用药提供依据。 1 材料与方法 1.1 供试农药 市售41%农达水剂(美国孟山都公司),72%2,4－D丁酯水剂(河北万全农药厂),盖草能(中国农业生产资料集团公司分装),2%使它隆乳油(美国陶氏益农公司),20%克无踪水溶液(英国卜内门化学工业有限公司),50%高特克悬浮剂(德国艾格福公司),50%扑草劲可湿性粉剂(昆明农药厂)和10%新代力可湿性粉剂(浙江省乐清市乐吉化工厂)共8种。 1.2 供试蚕品种 春季781×782*734,秋季苏3*秋3×苏4*苏12,由省农科院蚕研所春用组提供。 1.3 试验地点及桑品种 在本所生理病理室和桑树研究室进行,6年生油桑品种,低干养成,7500株／ha  相似文献   

Systematic localization of common disease-associated variation in regulatory DNA     

MT Maurano  R Humbert  E Rynes  RE Thurman  E Haugen  H Wang  AP Reynolds  R Sandstrom  H Qu  J Brody  A Shafer  F Neri  K Lee  T Kutyavin  S Stehling-Sun  AK Johnson  TK Canfield  E Giste  M Diegel  D Bates  RS Hansen  S Neph  PJ Sabo  S Heimfeld  A Raubitschek  S Ziegler  C Cotsapas  N Sotoodehnia  I Glass  SR Sunyaev  R Kaul  JA Stamatoyannopoulos 《Science (New York, N.Y.)》2012,337(6099):1190-1195

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水浴法提取怀菊花总黄酮工艺研究     

斯琴格日乐  恩德  陈阳 《广东农业科学》2012,39(7):113-115

以芦丁为对照品,正交设计优化怀菊花中总黄酮水浴提取条件,于505 nm处采用紫外分光光度法测定总黄酮的含量。结果表明,总黄酮在0～50μg/mL范围内,吸光度与浓度呈良好的线性关系,水浴法提取怀菊花总黄酮的最佳提取条件为A1B1C3,即温度60℃、乙醇浓度60%、料液比1∶10、提取2次、提取1 h,提取率为109.74 mg/g。回归方程为:y=0.01058x-0.01233,R2=0.9973,平均加标回收率为98.16%,RSD为0.210%。该方法成本低,无污染,操作简便,结果准确,可作为怀菊花的质量控制方法。  相似文献   

Percutaneous absorption between frog species: Variability in skin may influence delivery of therapeutics     

Victoria K. Llewelyn  Lee Berger  Beverley D. Glass 《Journal of veterinary pharmacology and therapeutics》2020,43(1):91-95

Frogs have permeable skin, so transdermal delivery provides a practical alternative to traditional dosing routes. However, little is known about how frog skin permeability differs interspecifically, and there are different reported clinical outcomes following topical application of the same chemical in different frog species. This study collated in vitro absorption kinetic data previously reported for two frog species: the green tree frog (Litoria caerulea) and the cane toad (Rhinella marina), and used linear mixed-effects modelling to produce a model of absorption. Histology of skin samples from each species was performed to observe morphological differences that may affect absorption. Absorption kinetics differed significantly between species, with the logP of the applied chemical a better predictor of permeability than molecular weight. Application site also influenced permeability, with dorsal permeability consistently higher in cane toads. Ventral permeability was more consistent between species. Skin thickness differed between species and skin regions, and this may explain the differences in absorption kinetics. Guidelines for selecting chemicals and dosing site when treating frogs are presented. The permeability differences identified may explain the poor reproducibility reported in the treatment of disease across frog species, and reinforces the importance of considering interspecies differences when designing therapeutic treatments for frogs.  相似文献   

Tektites and the age paradox in mid-pleistocene china     

Koeberl C  Glass BP  Keates SG  Potts R  Weiwen H  Yamei H  Deino A  Baoyin Y  Zhengtang G  Clark J 《Science (New York, N.Y.)》2000,289(5479):507a

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Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome   总被引：2，自引：0，他引：2  

Gibson DG  Benders GA  Andrews-Pfannkoch C  Denisova EA  Baden-Tillson H  Zaveri J  Stockwell TB  Brownley A  Thomas DW  Algire MA  Merryman C  Young L  Noskov VN  Glass JI  Venter JC  Hutchison CA  Smith HO 《Science (New York, N.Y.)》2008,319(5867):1215-1220

We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments.  相似文献   

The Academic Scientist, 1940-1960     

Glass B 《Science (New York, N.Y.)》1960,132(3427):598-603

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