Sequential bacteriological observations in relation to cell-mediated and humoral antibody responses of cattle infected with Mycobacterium paratuberculosis and maintained on normal or high iron intake   总被引：3，自引：0，他引：3  

AWD LEPPER  CR WILKS†  M. KOTIW†  JT WHITEHEAD†  KS SWART† 《Australian veterinary journal》1989,66(2):50-55

Twenty calves were orally infected with Mycobacterium paratuberculosis before weaning. Ten of these plus 4 non-infected controls were maintained on elevated dietary iron intake from 6 to 33 months of age. During this time, in which the majority of animals were bred, the influence of increased dietary iron upon tests of cellular and humoral immune responsiveness to antigens of the organism were monitored. Results were examined in relation to the organism's capacity to multiply and infect up to 7 portions of the intestinal tract. No significant differences were detected in the degree of intestinal disease or pattern of faecal excretion of M. paratuberculosis in iron supplemented and non-supplemented cattle. Cutaneous delayed-type hypersensitivity (DTH) to johnin PPD developed at 1 month and in-vitro lymphocyte and immunostimulatory activity (LS) to this antigen at 2 months after infection. LS indices were significantly reduced in magnitude in iron-supplemented cattle (p less than 0.01). Most ELISA antibody responses were positive 10 to 17 months after infection and preceded the fewer number of CF responses by several months. Neither of the antibody tests was affected by elevated iron intake. Generally, complete or partial resistance to paratuberculosis was associated with sustained positive monthly LS tests (index greater than or equal to 2.0), whereas antibody levels tended to be sustained only in the more severely affected cattle. Although neither test system was affected by pregnancy the ELISA failed to detect a significant proportion of cattle chronically shedding M. paratuberculosis in faeces.  相似文献   

Effects of triazole inhibitors of sterol biosynthesis on the free and esterified sterol composition of celery cell suspension cultures     

Philip J. Yates  John R. Lenton  L. John Goad 《Pest management science》1993,39(4):257-265

Treatment of celery cell suspension cultures with paclobutrazol and three other triazoles resulted in decreased growth and an accumulation of 14α-methylsterols in both the free sterol and the steryl ester pools, thus indicating that the triazoles were inhibiting the action of the plant obtusifoliol 14α-demethylase system. Obtusifoliol, 14α-methylcampesta-8,24(24¹)-dien-3β-ol and 14α-methylcampest-8-en-3β-ol were the main 14α-methylsterols to increase in the free sterol pool. In the presence of the triazoles the steryl esters became virtually depleted of sitosterol, campesterol, stigmasterol and isofucosterol, which were replaced by 14α-methylsterols with obtusifoliol becoming the dominant esterified sterol. Treatment of the celery culture with a fourth triazole which did not have a noticeable effect on growth and caused negligible accumulation of 14α-methylsterols in the free sterol pool nevertheless produced some enrichment of the steryl esters in obtusifoliol. The results indicate that, following triazole treatment, a rapid esterification of the accumulating 14α-methylsterol intermediates occurs before they start to build up in significant amount in the free sterol pool.  相似文献   

GenBank Status Report   总被引：1，自引：0，他引：1  

Burks C  Fickett J  Goad W 《Science (New York, N.Y.)》1987,235(4786):267-268

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Efficacy of zeta-cypermethrin as pour-on or spray formulations for the control of buffalo fly (Haematobia irritans exigua) in cattle     

JT ROTHWELL  KC HACKET  M. FRIEND  WR FARNSWORTH  LB LOWE 《Australian veterinary journal》1998,76(9):610-612

Objective To determine the efficacy of zeta-cypermethrin in controlling buffalo fly ( Haematobia irritans exigua ).
Design Five field trials in northern and central Queensland.
Procedure Zeta-cypermethrin pour-on at 2.5 mg/kg, spray at 62.5 ppm, deltamethrin pour-on and pour-on vehicle were applied to groups of 20 cattle. Buffalo fly counts were conducted three times before treatment and 3, 7, 14, 21, 28 and 35 days after treatment.
Results In central Queensland where synthetic pyrethroid resistance in buffalo fly populations was rare, 2.5 mg/kg of zeta-cypermethrin pour-on gave good control of buffalo fly for 4 weeks and was better than a deltamethrin product. A zeta-cypermethrin spray used at 62.5 ppm gave 14 days control. In far-north Queensland where resistance to synthetic pyrethroids and heavy rain was common, the maximum period of efficacy of zeta-cypermethrin pour-on was reduced to 2 weeks.
Conclusion In areas where there is low resistance to synthetic pyrethroids among buffalo flies, zeta-cypermethrin pour-on can be expected to give good control for 4 weeks.  相似文献   

Clinical effects of the use of a bipolar vessel sealing device for soft palate resection and tonsillectomy in dogs,with histological assessment of resected tonsillar tissue          下载免费PDF全文

DA Cook  PA Moses  JT Mackie 《Australian veterinary journal》2015,93(12):445-451

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Influence of Ovulation Status,Seasonality and Embryo Transfer Method on Development of Cloned Porcine Embryos     

OJ Koo  JT Kang  DK Kwon  HJ Park  BC Lee 《Reproduction in domestic animals》2010,45(5):773-778

To improve pig cloning efficiency, the present study evaluated the effect of ovulation status, seasonality and embryo transfer (ET) method on in vivo development of cloned porcine embryos. Cloned embryos were transferred to surrogate mothers on the same day of somatic cell nuclear transfer. In pre‐ovulation stage (PO), pregnancy rate (PR) and delivery rate (DR) were 36.3% and 9.4%, respectively. In post‐ovulation stage, 22.7% PR and 2.1% DR were recorded (both PR and DR are significantly higher in PO). When ET was performed during winter (December–February), spring (March–May), summer (June–August) and autumn (September–November), the PRs were 13.4%, 37.3%, 24.6% and 51.0%, while DRs were 0%, 12.7%, 4.3% and 7.8%, respectively. The highest PRs were recorded in autumn groups. However, DRs were significantly lower in autumn (7.8%) group compared with spring (12.7%) group. The PR was the lowest and no piglets were born in winter group, which might be because of the effect of low temperature during ET. To overcome the low PR in winter group, 0.25 ml straws were used for ET to minimize exposure time of embryos to ambient temperature. The straw ET group showed significantly higher PR in the winter group (23. 9%) compared with the conventional catheter‐loading group (7.7%). We suggest that using PO recipient and ET in spring is the best condition for pig cloning. In addition, alternative method to reduce cold shock during ET in winter is necessary.  相似文献   

Feasibility of infrared spectroscopy with pattern recognition techniques to identify a subpopulation of mares at risk of producing foals diagnosed with failure of transfer of passive immunity     

CB Riley  JT McClure  S Low‐Ying  BK Dolenko  RL Somorjai  RA Shaw 《Australian veterinary journal》2012,90(10):387-391

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Modular hybrid total hip arthroplasty. Experimental study in dogs     

Bruno W Minto  Cláudia Valéria S Brand?o  Gilberto JC Pereira  Daniela Campagnol  Maria Jaqueline Mamprim  Carlos Roberto Padovani  José JT Ranzani 《Acta veterinaria Scandinavica》2011,53(1):46

Background

This prospective experimental study evaluated the surgical procedure and results of modular hybrid total hip arthroplasty in dogs.

Methods

Ten skeletally mature healthy mongrel dogs with weights varying between 19 and 27 kg were used. Cemented modular femoral stems and uncemented porous-coated acetabular cups were employed. Clinical and radiographic evaluations were performed before surgery and at 30, 60, 90, 120, 180 and 360 days post-operation.

Results

Excellent weight bearing was noticed in the operated limb in seven dogs. Dislocation followed by loosening of the prosthesis was noticed in two dogs, which were therefore properly treated with a femoral head osteotomy. Femoral fracture occurred in one dog, which was promptly treated with full implant removal and femoral osteosynthesis.

Conclusions

The canine modular hybrid total hip arthroplasty provided excellent functionality of the operated limb.  相似文献   

Evaluation of a mobile phone plant nitrogen recommendation application in the greenhouse     

Lance Swearengin  Hardeep Singh  Carla Goad 《Journal of plant nutrition》2013,36(20):2615-2625

Abstract

Mobile phone applications have been developed to increase nutrient use efficiency. The objective of this study was to evaluate the efficiency of a mobile phone application known as “Plant Nitrogen Recommendation” for recommending supplemental fertilizer application for Stachys “Helene Von Stein,” Verbena “Homestead Purple,” Hibiscus “Aphrodite,” and Clethra “Hummingbird” using SPAD and atLEAF sensor readings. “Hummingbird” was less responsive to fertilizer correction groups due to the fact the application gave a fertilizer recommendation too late. “Helene Von Stein” and “Aphrodite” provided quick response to the correction group and produced the same as the top performing fertilizer level. No correction group was applied for “Homestead Purple” as SPAD and atLEAF critical value was never reached for the 0?g treatment. Thus, use of a mobile application can be recommended for N recommendations in “Helene Von Stein” and “Aphrodite” during production.  相似文献   

Preliminary study of faecal cortisol and corticosterone as an index of acute cortisol secretion in the koala (Phascolarctos cinereus)     

SD Johnston  RA Booth  M Pyne  T Keeley  JT Mackie  L Hulse  W Ellis 《Australian veterinary journal》2013,91(12):534-537

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