Validation of a Field Based Chromatin Dispersion Assay to Assess Sperm DNA Fragmentation in the Bottlenose Dolphin (Tursiops truncatus)     

M‐J Sánchez‐Calabuig  C López‐Fernández  E Martínez‐Nevado  JF Pérez‐Gutiérrez  J de la Fuente  SD Johnston  D Blyde  K Harrison  J Gosálvez 《Reproduction in domestic animals》2014,49(5):761-768

Over the last two decades, there have been significant advances in the use of assisted reproductive technology for genetic and reproductive management of captive dolphin populations, including evaluation of sperm DNA quality. This study validated a customized sperm chromatin dispersion test (SCDt) for the bottlenose dolphin (Tursiops truncatus) as a means of assessing sperm DNA damage both in the field and in the laboratory. After performing the SCDt, two different sperm morphotypes were identified: (i) sperm with fragmented DNA showed large haloes of dispersed DNA fragments emerging from a compact sperm nucleoid core and (ii) sperm containing non‐fragmented DNA displayed small compact haloes surrounded by a dense core of non‐dispersed DNA and protein complex. Estimates of sperm DNA fragmentation by means of SCDt were directly comparable to results obtained following a two‐tailed comet assay and showed a significant degree of correlation (r = 0.961; p < 0.001). This investigation also revealed that the SCDt, with minor modifications to the standard protocol, can be successfully conducted in the field using a LED florescence microscopy obtaining a high correlation (r = 0.993; p = 0.01) between the data obtained in the laboratory and in the field.  相似文献   

Spatial Response of Mammals to Late Quaternary Environmental Fluctuations     

RW Graham  EL Lundelius  MA Graham  EK Schroeder  RS Toomey  E Anderson  AD Barnosky  JA Burns  CS Churcher  DK Grayson  RD Guthrie  CR Harington  GT Jefferson  LD Martin  HG McDonald  RE Morlan  HA Semken  SD Webb  L Werdelin  MC Wilson 《Science (New York, N.Y.)》1996,272(5268):1601-1606

Analyses of fossil mammal faunas from 2945 localities in the United States demonstrate that the geographic ranges of individual species shifted at different times, in different directions, and at different rates in response to late Quaternary environmental fluctuations. The geographic pattern of faunal provinces was similar for the late Pleistocene and late Holocene, but differing environmental gradients resulted in dissimilar species composition for these biogeographic regions. Modern community patterns emerged only in the last few thousand years, and many late Pleistocene communities do not have modern analogs. Faunal heterogeneity was greater in the late Pleistocene.  相似文献