Intra-abdominal actinomycetoma in a cat     

Mellora J. Sharman  Clara S. Goh  Richard G. Kuipers von Lande  Jennifer L. Hodgson 《Journal of Feline Medicine and Surgery》2009,11(8):701-705

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Detection and characterization of Leptospira spp. in dogs diagnosed with kidney and/or liver disease in Selangor,Malaysia     

Sabri A. Rahman  Kuan H. Khor  Siti Khairani-Bejo  Seng F. Lau  Mazlina Mazlan  Azri Roslan  Soon H. Goh 《Journal of veterinary diagnostic investigation》2021,33(5):834

Leptospirosis is a serious bacterial disease that affects both humans and animals. A wide range of symptoms have been described in humans; the disease in dogs is commonly associated with kidney and/or liver disease. In Malaysia, information about the common serovars infecting dogs is limited. Therefore, we investigated the occurrences of leptospirosis in 124 pet dogs diagnosed with kidney and/or liver disease. Blood, urine, abdominal effusion, and/or kidney and liver were collected from the dogs. Based on microscopic agglutination testing, 53 of 124 (42.7%) dogs were seropositive for leptospiral exposure. Sera were frequently positive to serovars Bataviae (n = 12), Javanica (n = 10), and Icterohaemorrhagiae (n = 10). Direct detection using PCR showed that 42 of 124 (33.9%) of the whole blood and 36 of 113 (31.9%) urine samples were positive for pathogenic Leptospira spp. By PCR, 2 of 23 (9.1%) kidney and 2 of 23 (9.1%) liver were positive for pathogenic Leptospira spp. Abdominal effusion from 4 dogs were PCR-positive for pathogenic Leptospira spp. The species detected were L. interrogans, L. borgpetersenii, L. kirschneri, and L. kmetyi by partial 16S rRNA sequencing. We further identified and characterized 11 Leptospira spp. isolates from 8 dogs as serovars Bataviae, Javanica, and Australis. The mortality rate of the Leptospira-infected dogs was high (18 of 53; 34%).  相似文献   

Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti     

Hill JE  Gottschalk M  Brousseau R  Harel J  Hemmingsen SM  Goh SH 《Veterinary microbiology》2005,107(1-2):63-69

Streptococcus suis serotypes have traditionally been identified by morphology, biochemical profiling and serotyping. Analysis of the sequences of 16S rRNA and cpn60 genes of the 35 characterized serotypes of S. suis led to the observation that two serotypes 32 and 34, are significantly distinct from other S. suis serotypes and may represent a distinct species. Here we present DNA sequence data and biochemical profiles which indicate that S. suis serotypes 32 and 34, isolated from pigs, are clustered with Streptococcus orisratti, a Voges-Proskauer negative, alpha-haemolytic, aesculin-hydrolytic, Lancefield group A streptococcus isolated from the teeth of rats.  相似文献   

Effect of ammoniation of rapeseed meal on the sinapine content of the meal     

Y. K. Goh  A. Shires  A. R. Robblee  D. R. Glandinin 《British poultry science》1982,23(2):121-128

1. Two experiments were conducted to study the effects of ammoni‐ation of rapeseed meal on its sinapine content.

2. In the first experiment, five samples of a low‐glucosinolate meal (produced from Regent, Candle or Tower cultivars) and one sample of a high‐glucosinolate meal were eluted by one passage of ethanol ammoniated to concentrations of 0.2, 0.5 or 1.0 M NH₃ at the ratio of 2 1 ammoniated ethanol/kg meal. Elution with 1.0M ammoniated ethanol decreased the sinapine content of the meals by as much as 80%.

3. In the second experiment, four low‐glucosinolate meals (from Candle cultivar) from a pilot processing plant were used. Two of the meals were obtained by sparging during desolventising with or without anhydrous ammonia (50 g anhydrous ammonia/kg meal) and two of the meals were produced by sparging during desolventising with or without hydrous ammonia (50 g anhydrous ammonia and 50 g steam/kg meal). Ammonia with steam caused the greatest decrease (65%) in sinapine content.

4. Total glucosinolate and 5‐vinyl‐2‐oxazolidinethione concentrations in the meals were also decreased (17 to 35%) by the ammoniation treatments in experiment 2.  相似文献   

Phytoplankton uptake and growth rate in the Japanese egg cockle Fulvia mutica     

Sou Nagasoe  Tetsuji Hisada  Goh Nishitani  Hiroyuki Takeuchi  Kazutaka Sakiyama 《International Aquatic Research》2019,11(4):359-375

To clarify the relationship between the quantity of food ingested by and the growth rate of the Japanese egg cockle Fulvia mutica (Reeve), we conducted a laboratory breeding experiment for 2 weeks and estimated the chlorophyll a (chl-a) concentrations in water and the increments in shell length and soft-body weight of this species under five chl-a concentrations. Moreover, we compared the relationship between cockle growth (changes in soft-body weight and shell length) and their feeding environment observed in the laboratory experiment with the results of a field investigation conducted at two sites in the Sea of Japan, Kumihama Bay (35°38′5″ N, 134°54′00″ E) and Kunda Bay (35°33′30″ N, 135°15′4″ E). The changes in soft-body weight were similar in both laboratory and field investigations, but those in shell length were not. We, therefore, considered shell length changes as unsuitable for evaluating the relationship between growth and feeding in F. mutica. Based on the changes in soft-body weight, it was possible to classify the feeding environment of this species into the following three types: (1) < 1.52 μg chl-a L−1, negative feeding environment for cockle growth; (2) 1.52–5.71 μg chl-a L−1, neutral feeding environment for cockle growth; (3) > 5.71 μg chl-a L−1, positive feeding environment for cockle growth (growth increased with increasing chl-a concentration up to about 11 μg chl-a L−1). These results indicate that maintaining chl-a concentration in the breeding water within 5.71–11 μg chl-a L−1 is desirable for rearing Japanese egg cockle.  相似文献   

狐狸巴氏杆菌病病原的分离与鉴定     

李玉梅  姚纪元 《中国兽药杂志》2007,41(10):56-57

巴氏杆菌病又叫出血性败血症，简称出败，主要是由多杀性巴氏杆菌所引起的各种畜禽和野生动物感染的一种传染病。临床特征急性病例主要表现为败血症和炎性出血，慢性特征表现为皮下组织、关节、各脏器的局灶性化脓性炎症。该病常在畜禽中发生，而在狐狸等经济动物方面报道相对较少。2006年秋，吉林左家某狐场的狐狸突然发病并陆续死亡，经流行病学调查、病理剖检和实验室检验，确诊为多杀性巴氏杆菌感染所致。  相似文献   

Anesthesia and acoustic stress-induced intra-uterine growth retardation in mice   总被引：2，自引：0，他引：2  

Haque SF  Izumi S  Aikawa H  Suzuki T  Matsubayashi H  Murano T  Kika G  Ikeda M  Goya K  Makino T 《The Journal of reproduction and development》2004,50(2):185-190

Stress interferes with reproduction, adversely influencing implantation and fetal growth, and sometimes even leading to abortion. Here, we attempted to evaluate the early gestational effects of uncomfortable sound on pregnant mice and their offspring. Ten-week-old pregnant Jcl:ICR mice were exposed to sound (100 dB, random frequency between 9-34 kHz) for 8 hours on the 3(rd), 5(th) and 7(th) gestational days (GD). The effects of general anesthesia were also investigated, with or without acoustic stress. All groups were examined on the 18(th) GD for fetal growth. Fetal weight, number of ossified sacrococcygeal vertebrae and placental weight were all significantly reduced (P<0.0001) when stress was induced on the 7(th) GD, but not on the 3(rd) or 5(th) GD. This intra-uterine growth retardation (IUGR) was significantly inhibited by general anesthesia (P<0.0001), although general anesthesia alone induced significant IUGR (P<0.0001) when compared with control mice. This suggests that acoustic exposure indirectly exerts an effect on fetal growth, possibly via a psycho-maternal pathway. We also found that analysis of the number of ossified sacrococcygeal vertebrae is the most sensitive tool for the study of IUGR.  相似文献   

A pertussis toxin-sensitive G protein in hippocampal long-term potentiation   总被引：7，自引：0，他引：7  

J W Goh  P S Pennefather 《Science (New York, N.Y.)》1989,244(4907):980-983

High-frequency (tetanic) stimulation of presynaptic nerve tracts in the hippocampal region of the brain can lead to long-term synaptic potentiation (LTP). Pertussis toxin prevented the development of tetanus-induced LTP in the stratum radiatum-CA1 synaptic system of rat hippocampal slices, indicating that a guanosine triphosphate-binding protein (G protein) may be required for the initiation of LTP. This G protein may be located at a site distinct from the postsynaptic neuron (that is, in presynaptic terminals or glial cells) since maximal activation of CA1 neuronal G proteins by intracellular injection of guanosine-5'-O-(3-thiotriphosphate), a nonhydrolyzable analog of guanosine 5'-triphosphate, did not occlude LTP.  相似文献   

Associative induction of posttetanic and long-term potentiation in CA1 neurons of rat hippocampus   总被引：6，自引：0，他引：6  

B R Sastry  J W Goh  A Auyeung 《Science (New York, N.Y.)》1986,232(4753):988-990

Electrical stimulation of fibers in the stratum radiatum causes an excitatory postsynaptic potential in CA1 neurons of the hippocampus. Other excitatory inputs to or direct depolarization of these CA1 neurons during stimulation of the stratum radiatum caused a subsequent increase in the excitatory postsynaptic potential. This enhancement was characterized as a brief potentiation (2 to 3 minutes, similar to posttetanic potentiation) and a long-term potentiation (presumed to be involved in learning and memory). These potentiations are probably induced by an interaction of the postsynaptic cell or other presynaptic terminals with the test presynaptic terminals.  相似文献   

Analysis of sulfur-containing components of a soil treated with simulated acid rain     

Gary W. Vanloon  George W. Hay  Raymond H-T. Goh 《Water, air, and soil pollution》1987,34(3):233-240

Samples of the LFH and Bfh horizons of an Orthic Humo-Ferric Podzol were analyzed for S components after irrigation with simulated acid rain solutions of pH 5.7, 3.5, and 2.0 for 720 days. Organic S was preponderant. In the LFH horizon, the mass ratio of ester sulfate: carbon-bonded S was approximately I : 1 for samples treated with solutions of pH 5.7 and 3.5; for the sample treated with the pH-2.0 solution, the ratio was about 2 : 1 and the concentrations of both inorganic sulfate and ester sulfate were markedly higher. In the Bth horizon, carbon-bonded S was the major form of organic S, except in the sample subjected to the high-acid (pH 2.0) simulated rain. The organic S components were further separated into chloroform-soluble, aqueous trifluoracetic acid-soluble, and residual fractions. Significant increases in inorganic sulfate, both water soluble and adsorbed were found after the pH-2.0 treatment.  相似文献