Water parameters affect anaesthesia induced by eugenol in silver catfish,Rhamdia quelen     

Diego Prestes Gomes  Brunele Weber Chaves  Alexssandro Geferson Becker  Bernardo Baldisserotto 《Aquaculture Research》2011,42(6):878-886

The present study investigated the effects of water pH (5.0, 7.0 and 9.0), hardness (0, 20 and 120 mg CaCO₃ L^?1) and temperature (15, 23 and 30 °C) on the induction of sedation and anaesthesia, and subsequent recovery, of silver catfish exposed to eugenol. Moreover, the blood gas tensions (PvO₂ and PvCO₂) and blood pH in silver catfish acclimated to these temperatures were investigated after exposure to eugenol. Water pH, hardness, temperature and fish size affect the efficacy of eugenol in silver catfish, particularly at the lower concentrations tested (20 and 30 mg L^?1). Sedation of this species can be induced at concentrations as low as 20 mg L^?1, but for anaesthesia, a concentration of at least 40 mg L^?1 of eugenol must be used to compensate for the influence of fish size and water quality. Blood gas tension and pH were affected by eugenol anaesthesia, but only in fish acclimated to 30 °C.  相似文献   

Benefits of using the probiotic Efinol^®L during transportation of cardinal tetra, Paracheirodon axelrodi (Schultz), in the Amazon     

Levy Carvalho Gomes  Richard Philip Brinn  Jaydione Luiz Marcon  Lucelle Araújo Dantas  Franmir Rodrigues Brandão  Janessa Sampaio de Abreu  Paulo Evandro Mendes Lemos  Dawn Michelle McComb  & Bernardo Baldisserotto 《Aquaculture Research》2009,40(2):157-165

The objective of this experiment was to test the probiotic Efinol^®L during transportation of cardinal tetra, Paracheirodon axelrodi (Schultz). For the transportation, fish were distributed in 18 plastic tanks, of which nine contained the Efinol^®L (10 mg L⁻¹; probiotic treatment) and the remaining had no probiotic (control treatment). Transport lasted 24 h and three different boxes of each treatment were sampled at 3, 12 and 24 h. Up to the 12-h sampling period, no significant difference in the survival was observed; on the other hand, survival was higher at the end of the transport (24 h) in the probiotic treatment. No significant difference was found in dissolved oxygen and temperature between treatments. Conductivity, pH and alkalinity increased along the transport, but without a difference between treatments. Ammonia increased in all treatments, although it was significantly lower in the probiotic group at 24 h. Cortisol levels were significantly higher in all transported fish when compared with the basal values. At 24 h, cortisol levels in control fish were significantly higher than those in the probiotic treatment. With the observed results, we are able to conclude that the probiotic Efinol^®L is efficient during cardinal transport, lowering the mortality and helping maintain water quality possibly by lowering metabolic wastes.  相似文献   

Impact of pond management on tambaqui, Colossoma macropomum (Cuvier), production during growth-out phase     

Levy Carvalho Gomes  & Clichenner Rodrigues Silva 《Aquaculture Research》2009,40(7):825-832

This study evaluated the impact of pond management on tambaqui, Colossoma macropomum (Cuvier), rearing during the growout phase. Juvenile tambaqui were stocked in ponds with three different management regimes: (1) natural ponds (Nat); (2) limed ponds (Lim); and (3) limed and bimonthly fertilized ponds (LimFer). The experiment lasted 210 days and the growth parameters were evaluated monthly. Water quality and effluent measurements were performed every 15 days and at the end of the experiment yield parameters were obtained. There was no difference in weight and length among treatments. Stomach contents and zooplankton availability were not influenced by pond management, but the rearing period had an influence on them. Food conversion rate (FCR) was better in fish from the Lim treatment than in fish from the Nat treatment. The pH, hardness and alkalinity values were significantly higher in the Lim and LimFer ponds, where the liming procedure was performed. The effluent analysis showed a more potentially impacting effluent in the LimFer treatment, where phosphorus and orthophosphate concentrations showed values significantly higher than those in Lim and Nat ponds. The results show that the Lim treatment is the best approach, as in this treatment fish achieve market size, better FCR, yield and have a reduced environmental impact.  相似文献   

Anabolism,catabolism and proteomic analysis in the slow‐twitch muscle of pacu (Piaractus mesopotamicus) submitted to prolonged fasting followed by refeeding     

Maria Laura Gabriel Kuniyoshi  Rafaela Nunes da Silva‐Gomes  Bruna Tereza Thomazini Zanella  Lucilene Delazari dos Santos  Maeli Dal‐Pai‐Silva 《Aquaculture Research》2020,51(3):1101-1112

Here, we aimed to study the slow muscle of the fish Piaractus mesopotamicus submitted to 30 days of fasting (D30) followed by 1 day (D31) or 30 days of refeeding (D60). Histological analysis of fibre diameter was performed in D30 and D60. The gene expression of parvalbumin (pvalb), atrogenes (murf1a, murf1b, mafbx) and anabolic genes (igf‐1, mtor) was analysed using RT‐qPCR in D30, D31 and D60. The proteome was obtained by shotgun proteomics at D30 and D60, and the set of differentially expressed proteins was analysed by bioinformatics. In all experiments, the control was regularly fed fish. The histological analysis showed no changes in muscle fibre diameter. The expression of catabolic and anabolic genes was not changed, except for the downregulation of igf‐1 in D30 and of mafbx in D31. The expression of pvalb was not changed in D30 and D60 but was decreased in D31. The proteomic analysis identified 169 proteins in D30 (24 upregulated and 18 downregulated) and 170 proteins in D60 (17 upregulated and 21 downregulated); many of them were related to energetic metabolism and intracellular Ca²⁺ homoeostasis. Overall, our results indicate that the slow‐twitch muscle presented few changes upon prolonged fasting and refeeding condition.  相似文献   

Trichlorfon acute lethal toxicity to juvenile tambaqui (Colossoma macropomum)     

Hallana Cristina Menezes da Silva  Andr Gentil da Silva  Jhonatan Júnior Silva Idalino  Fabrício Barros de Sousa  Ana Lúcia Silva Gomes  Wallice Paxiúba Duncan  Daniele Aparecida Matoso 《Aquaculture Research》2020,51(2):863-866

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A Study of Lipid Bioaccessibility in Canned Sardine (Sardina pilchardus) and Chub Mackerel (Scomber japonicus)     

T. Garcia  A. Gomes  C. Mesquita  S. Tanni 《Journal Of Aquatic Food Product Technology》2019,28(4):402-412

The lipid composition and bioaccessibility in canned sardine (Sardina pilchardus) and chub mackerel (Scomber japonicus) were studied. Triacylglycerols were absent from the bioaccessible fraction in both products and each FA had a different bioaccessibility level. While total fat bioaccessibility was in the 60–64% range, the bioaccessibility of ω6 PUFA and DHA was <55%. Bioaccessibility depended on canned fish product species: fat, EPA, DHA, and ω3 PUFA bioaccessibility levels seemed to be higher in canned sardine than in canned mackerel. Daily consumption of 278 g of canned mackerel or 47 g of canned sardine would ensure the recommended daily intake of EPA+ DHA.  相似文献   

Dietary organic acids blend alone or in combination with an essential oil on the survival,growth, gut/liver structure and de hemato‐immunological in Nile tilapia Oreochromis niloticus     

Kennya Gomes Silva Addam  Scheila Anelise Pereira  Gabriel Fernandes Alves Jesus  Lucas Cardoso  Nichola Syracuse  Gustavo Ruschel Lopes  Nicollas Breda Lehmann  Bruno Correa da Silva  Lúvia Souza de S  Francisco Clio Maia Chaves  Maurício Latera Martins  Jos Luiz Pedreira Mourio 《Aquaculture Research》2019,50(10):2960-2971

This study evaluated the effects of dietary supplementation of organic acids blend (OAB) alone or in combination with essential oil, Lippia origanoides (OAE) for Nile tilapia fed supplemented diets for 30 days. Fish (1.1 ± 0.04 g) were fed control (Control), or OAB 0.5% or OAB 0.5% + essential oil 0.125% (OAE) respectively. At the end of the experiment, samples were collected for de hemato‐immunological, histological analysis of the intestine and liver, as well as microbiology of the intestine. The pH of the diets supplemented with OAB and OAE reduced 0.92 and 0.19 respectively. The growth and FCR were unaffected by the treatments, but survival was significantly higher in the OAB treatment. Fish fed the OAB diet showed reduced concentration of total heterotrophic bacteria and Pseudomonas sp. in the intestine. Increased glucose in fish fed OAB and high number of circulating monocytes in fish fed OAE diet were observed. The anterior intestine of fish fed OAE diet showed larger number of goblet cells and increased villi height. The diet supplemented with OAB, mainly, improved the intestinal health and survival of tilapia juveniles and can be used in juvenile production.  相似文献   

Leaf gas exchange of green dwarf coconut (Cocos nucifera L. var. nana) in two contrasting environments of the Brazilian north-east region     

F. P. Gomes  M. S. Mielke  A. -A. F. de Almeida 《The Journal of Horticultural Science and Biotechnology》2013,88(6):766-772

Summary

Leaf gas exchange characteristics of green dwarf coconut were studied in two contrasting environments highlighting the technical aspects of construction of light-response curves in field conditions and its mathematical approach. Site I is at the south-east region of Bahia State in a hot and humid climate area. The contrasting Site II is located at the Pernambuco State in a semi-arid type of climate. Measurements of leaf gas exchange were accomplished using a portable photosynthesis system equipped with an artificial light source. Significant differences were observed between the two sites for the leaf-to-air water vapour pressure deficit and light-saturated (Qi ≥800.μmol m^–2 s^–1) net photosynthesis and stomatal conductance. Mean values of these variables, in that order, were 1.4 and 2.1 kPa, 12.7 and 8.3.μmol CO₂ m^–2 s^–1 and 0.36 and 0.13 mol H₂Om^–2 s^–1 at Sites I and II, respectively. Eight mathematical models relating both net photosynthesis and stomatal conductance with photosynthetically active radiation, were adjusted from the field data. Good results were obtained with the models, mainly when a linear function of leaf-to-air water vapour pressure deficit was included, indicating that the green dwarf coconut stomata show a strong dependence on water vapour pressure deficit.  相似文献   

Citral and linalool chemotypes of <Emphasis Type="Italic">Lippia alba</Emphasis> essential oil as anesthetics for fish: a detailed physiological analysis of side effects during anesthetic recovery in silver catfish (<Emphasis Type="Italic">Rhamdia quelen</Emphasis>)     

Carine de Freitas Souza  Matheus Dellaméa Baldissera  Adriane Erbice Bianchini  Elisia Gomes da Silva  Rosa Helena Veraz Mourão  Lenise Vargas Flores da Silva  Denise Schmidt  Berta Maria Heinzmann  Bernardo Baldisserotto 《Fish physiology and biochemistry》2018,44(1):21-34

The viability using Lippia alba essential oil as an anesthetic for fish was studied, particularly with respect to physiological effects during recovery. Anesthesia of silver catfish (Rhamdia quelen) using 100 and 300 μL L^?1 of two different chemotypes of L. alba essential oil (citral EO-C and linalool EO-L) prevented the increase of plasma cortisol levels caused by handling, but did not avoid alterations in energetic metabolism. Silver catfish did not have increased the levels of thiobarbituric acid reactive species in the kidney and liver during recovery after anesthesia with either EO, avoiding lipid damage. On the other hand, fish anesthetized with EO-C showed higher protein carbonylation levels, superoxide dismutase, catalase, and glutathione S-transferase activities and non-protein thiol group levels in both tissues compared to controls. Our results suggest that both oils show antioxidant capacity, but anesthesia with EO-L does not cause damage to lipids or proteins, only temporary changes, typical of physiological adjustments during recovery from anesthesia. Therefore, EO-L is an effective anesthetic for silver catfish with fewer side effects than EO-C.  相似文献   

Loss of genetic variability in the captive stocks of tambaqui,Colossoma macropomum (Cuvier, 1818), at breeding centres in Brazil,and their divergence from wild populations          下载免费PDF全文

Jonas da Paz Aguiar  Paola Fabiana Fazzi Gomes  Igor Guerreiro Hamoy  Sidney Emanuel Batista dos Santos  Horacio Schneider  Iracilda Sampaio 《Aquaculture Research》2018,49(5):1914-1925

The loss of variability in farmed populations and the risks of interactions with wild populations support the need for the genetic monitoring of species farmed throughout the world. In Brazil, the tambaqui is the most widely farmed native fish species. Despite this, there are no data on the pedigree of the farmed stocks, and the potential for interactions with wild populations in the Amazon basin has raised concerns with regard to the genetic variability of these stocks. The present study analysed sequences of the mitochondrial Control Region and 12 microsatellites to characterize the genetic variability of seven historically important commercial tambaqui breeding centres located in four different regions of Brazil, and compared these sequences with those obtained from individuals collected from a wild population. High levels of genetic diversity were found in the wild population, whereas genetic diversity was reduced in both markers in most captive populations, except for the broodstock located near the Amazon River. High F_ST and D_EST indices were recorded between the wild population and most of the captive stocks analysed. The drastic reduction in genetic diversity found in most captive stocks and the difference between these stocks and the wild population may have been the result of the small size of the founding populations and the absence of breeding management. The renewal of the broodstocks and the application of breeding management techniques are recommended. In the Amazon region, in addition, the use of broodstocks that are genetically very different from local wild populations should be avoided.  相似文献